Genomic and antigenic variation among rotavirus strains circulating in a large city of Argentina.

Abstract:

:Knowledge of the antigenic diversity of rotaviruses circulating in a region should be acquired before introducing a rotavirus vaccine. In a collection of 151 rotavirus-positive samples from Mendoza, Argentina, strain diversity was evaluated utilizing G-typing monoclonal antibodies (MAbs), reverse-transcriptase-polymerase chain reaction (RT-PCR) G and P typing, and electropherotyping (PAGE). The G type of 137 (91%) specimens was determined. Typing MAb reactivity with the homologous type ranged from 25-94%. For the seven G1 MAbs utilized, 28 patterns of reactivity among 68 G1 strains occurred. For the 48 G2 strains, six patterns of reactivity occurred utilizing three G2-specific MAbs. Of the 92 samples G- and P-typed by reverse-transcriptase-polymerase chain reaction, 89% had single G/P combinations: eight G1[P4], one G1[P6], twelve G1[P8], 58 G2 [P4], and two G2 [P6]. Nine samples had more than one G type with a single P type, one sample had two P types associated with one G type, and one sample contained multiple G and P types. Twenty-nine PAGE patterns occurred for all G types, but differences of antigenic reaction did not predict differences in migration of gene segments 7, 8, and 9. For three specimens showing discordant results between G type by enzyme-linked immunosorbent assay (EIA) and RT-PCR, we observed unexpected electropherotypes. Complementary evaluation by RT-PCR and MAb-based EIA with multiple typing MAbs revealed genetic and antigenic diversity of circulating rotaviruses, including extensive intratypic variation of the G1 and G2 neutralization antigens, in Mendoza during a single season of rotavirus activity.

journal_name

J Med Virol

authors

Espul C,Cuello H,Martinez N,Centorbi O,O'Ryan M,Jackson L,Campos F,Matson DO

keywords:

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

504-9

issue

4

eissn

0146-6615

issn

1096-9071

pii

10.1002/1096-9071(200008)61:4<504::AID-JMV14>3.0.C

journal_volume

61

pub_type

杂志文章
  • Changing distribution of norovirus genotypes and genetic analysis of recombinant GIIb among infants and children with diarrhea in Japan.

    abstract::A total of 402 fecal specimens collected during July 2003-June 2004 from infants and children with acute gastroenteritis, encompassing five localities (Maizuru, Tokyo, Sapporo, Saga, and Osaka) of Japan, were tested for the presence of norovirus by RT-PCR. It was found that 58 (14.4%) fecal specimens were positive for...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20649

    authors: Phan TG,Kuroiwa T,Kaneshi K,Ueda Y,Nakaya S,Nishimura S,Yamamoto A,Sugita K,Nishimura T,Yagyu F,Okitsu S,Müller WE,Maneekarn N,Ushijima H

    更新日期:2006-07-01 00:00:00

  • Neutralization test for BK virus: plaque reduction detected by immunoperoxidase staining.

    abstract::We developed an immunoperoxidase staining test to detect structural antigens of BK virus (BKV) in Vero cell cultures. This test was used to examine the neutralizing activity of human and immunized animal sera. It was shown that sera positive for BKV antibodies measured by hemagglutination inhibition test and enzyme-li...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890190311

    authors: Flaegstad T,Traavik T,Christie KE,Joergensen J

    更新日期:1986-07-01 00:00:00

  • Non-isotopic detection of hepatitis C virus quasispecies by single strand conformation polymorphism.

    abstract::In patients infected with the hepatitis C virus (HCV), a heterogeneous population of viruses, so-called quasispecies exists in vivo. The hypervariable regions (HVR) within the second envelope gene (HCV-E2) show particularly highly intratypic variability and are considered to be the target of neutralizing antibodies. T...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199711)53:3<245::aid-jmv11

    authors: Lee JH,Stripf T,Roth WK,Zeuzem S

    更新日期:1997-11-01 00:00:00

  • Induction of apoptosis in HT-29 cells infected with SA-11 rotavirus.

    abstract::Rotavirus infection is associated both in vivo and in vitro with a series of subcellular pathological alterations leading to cell lysis. It has been suggested that these modifications can play a key role in the pathogenesis of rotavirus-associated diarrheal disease. We describe the effects of SA-11 rotavirus infection...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1096-9071(199612)50:4<325::AID-JMV8>

    authors: Superti F,Ammendolia MG,Tinari A,Bucci B,Giammarioli AM,Rainaldi G,Rivabene R,Donelli G

    更新日期:1996-12-01 00:00:00

  • Algorithm based on CMV kinetics DNA viral load for preemptive therapy initiation after hematopoietic cell transplantation.

    abstract::Preemptive therapy in hematopoietic cell transplantation is initiated by a diagnostic technique at first detection of cytomegalovirus (CMV). The aim of this study was to use the viral dynamics of CMV DNA viral to start preemptive therapy, and to prospectively compare the CMV viral load kinetics to pp65 antigenemia. Tw...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21996

    authors: Halfon P,Berger P,Khiri H,Martineau A,Pénaranda G,Merlin M,Faucher C

    更新日期:2011-03-01 00:00:00

  • Quantification of infectious duck hepatitis B virus by radioimmunofocus assay.

    abstract::A simple method is described for the precise quantification of infectious duck hepatitis B virus (DHBV) in cell culture, using a radioimmunofocus assay (RIFA). Primary duck hepatocyte cell cultures were infected with serial dilutions of viral samples as for a plaque assay, but then maintained with liquid overlay mediu...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Anderson DA,Grgacic EV,Luscombe CA,Gu X,Dixon R

    更新日期:1997-08-01 00:00:00

  • Identification of a human group C rotavirus in Malaysia.

    abstract::Stool specimens from 334 infants and young children hospitalized with diarrhea in the General Hospital, Kuala Lumpur, Malaysia between August and November, 1987 were analyzed for the presence of rotavirus double-stranded (ds) RNA by polyacrylamide gel electrophoresis. Of the 334 specimens analyzed, 32 (9.6%) were posi...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890430302

    authors: Rasool NB,Hamzah M,Jegathesan M,Wong YH,Qian Y,Green KY

    更新日期:1994-07-01 00:00:00

  • Detection of a human rotavirus strain different from types 1 and 2--a new subgroup? Epidemiology of subgroups in a Swedish and an Ethiopian community.

    abstract::A possible new subgroup of human rotavirus was found by crossed immunoelectrophoresis and enzyme-linked immunosorbent assay. The epidemiology of the established subgroups 1 and 2 and this new variant was studied in two different communities. Of 398 rotavirus isolates from Malmö, Sweden, 26.8% were of type 1, 71.7% of ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890120205

    authors: Tufvesson B

    更新日期:1983-01-01 00:00:00

  • Titration of hepatitis B virus infectivity in the sera of pre-acute and late acute phases of HBV infection: transmission experiments to chimeric mice with human liver repopulated hepatocytes.

    abstract::Studies of hepatitis B virus (HBV) infection in non-human primates such as chimpanzees are no longer possible due to ethical considerations and the endangered status of chimpanzees since April 2007 in Japan. A human hepatocyte transplanted chimeric mouse was used to characterize HBV infectivity in serial stages of acu...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21320

    authors: Tabuchi A,Tanaka J,Katayama K,Mizui M,Matsukura H,Yugi H,Shimada T,Miyakawa Y,Yoshizawa H

    更新日期:2008-12-01 00:00:00

  • HPV E6/E7 mRNA versus HPV DNA biomarker in cervical cancer screening of a group of Macedonian women.

    abstract::High risk types of human papillomaviruses E6/E7 oncogenes and their association with tumor suppressor genes products are the key factors of cervical carcinogenesis. This study proposed them as specific markers for cervical dysplasia screening. The aim of the study is to compare the clinical and prognostic significance...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24199

    authors: Duvlis S,Popovska-Jankovic K,Arsova ZS,Memeti S,Popeska Z,Plaseska-Karanfilska D

    更新日期:2015-09-01 00:00:00

  • MIP-1 alpha and MIP-1 beta induction by dengue virus.

    abstract::Dengue virus (DV) infection can result in either a mild febrile illness known as dengue fever (DF) or a life-threatening disease called dengue hemorrhagic fever (DHF). DHF is more prevalent in patients undergoing secondary DV infection. This observation has led to the hypothesis that DHF may be the result of immune re...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.2037

    authors: Spain-Santana TA,Marglin S,Ennis FA,Rothman AL

    更新日期:2001-10-01 00:00:00

  • Molecular and epidemiological characteristics of blood-borne virus infections among recent immigrants in Spain.

    abstract::The increased immigration from developing regions to Western countries raises public health concerns related to blood-borne viruses. The prevalence of human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), and human T-lymphotropic virus (HTLV) infections among recent immigrants attending...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20744

    authors: Toro C,Jiménez V,Rodríguez C,Del Romero J,Rodés B,Holguín A,Alvarez P,García-Campello M,Gómez-Hernando C,Guelar A,Sheldon J,de Mendoza C,Simón A,Soriano V

    更新日期:2006-12-01 00:00:00

  • Altered expression of CXCR3 and CCR6 and their ligands in HTLV-1 carriers and HAM/TSP patients.

    abstract::Recruitment of leukocytes by chemokines and chemokine receptors to CNS plays a crucial role in the induction of inflammatory response in HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). In the present study, chemokine and chemokine receptors involved in trafficking of lymphocytes to the CNS were me...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24779

    authors: Rafatpanah H,Felegari M,Azarpazhooh MR,Vakili R,Rajaei T,Hampson I,Hassanshahi G,Valizadeh N,Gerayli S,Hamid F,Zamanian S,MollaHosseini F,Rezaee SA

    更新日期:2017-08-01 00:00:00

  • Towards developing HIV-2 lentivirus-based retroviral vectors for gene therapy: dual gene expression in the context of HIV-2 LTR and Tat.

    abstract::Because of the distinct ability of retroviruses to integrate into the target cell genome and thus achieve long-term expression, retrovirus vectors hold great promise for stable gene transfer. Such vectors derived from human immunodeficiency retroviruses (HIVs) and other lentiviruses are envisioned to possess several a...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199802)54:2<118::aid-jmv9>

    authors: Sadaie MR,Zamani M,Whang S,Sistron N,Arya SK

    更新日期:1998-02-01 00:00:00

  • Comparative efficacy, pharmacokinetic, pharmacodynamic activity, and interferon stimulated gene expression of different interferon formulations in HIV/HCV genotype-1 infected patients.

    abstract::The effect of different formulations of interferon on therapeutic response in patients coinfected with HIV and HCV is unclear. In this study, the safety, tolerability, viral kinetics (VK) modeling and host responses among HIV/HCV coinfected patients treated with pegylated-IFN or albinterferon alfa-2b (AlbIFN) with wei...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23773

    authors: Osinusi A,Bon D,Nelson A,Lee YJ,Poonia S,Shivakumar B,Cai SY,Wood B,Haagmans B,Lempicki R,Herrmann E,Sneller M,Polis M,Masur H,Kottilil S

    更新日期:2014-02-01 00:00:00

  • Distribution of BK polyomavirus genotypes in Tunisian renal transplant recipients.

    abstract::BK polyomavirus (BKV) is a ubiquitous virus in humans that remains latent in the urogenital tract after a primary infection during childhood. The virus, which is reactivated frequently and excreted in urine, can cause nephropathy in renal transplant recipients. BKV sequences are classified into four subtypes (I-IV). S...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.22035

    authors: Boukoum H,Nahdi I,Foulongne V,Zallema D,Aloui S,Achour A,Segondy M,Aouni M

    更新日期:2011-04-01 00:00:00

  • Genetic characterization of human metapneumovirus identified through community and facility-based surveillance of infants in Dhaka, Bangladesh.

    abstract:BACKGROUND:Acute respiratory infection (ARI) is a leading cause of morbidity and mortality in children in low and middle-income countries. Human metapneumovirus (hMPV) is one of the most common viral etiological agents for ARIs in children. OBJECTIVES:In this study, we explored the genotypic diversity and the epidemio...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25351

    authors: Rahman MZ,Sumiya M,Sahabuddin M,Pell LG,Gubbay JB,Rahman R,Momtaz F,Azmuda N,Shanta SS,Jahan I,Rahman M,Mahmud AA,Roth DE,Morris SK

    更新日期:2019-04-01 00:00:00

  • Prevalence and risk factors of hepatitis B virus infection in Middle-Limburg Belgium, year 2017: Importance of migration.

    abstract:BACKGROUND AND AIM:The hepatitis B virus (HBV) prevalence study performed in 2003 in Belgium is believed to be underestimating HBV prevalence due to underrepresentation of the non-Belgian population. Therefore, we assessed the prevalence and risk factors of HBV infection in a multi-ethnic region situated in Middle-Limb...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25457

    authors: Koc ÖM,Kremer C,Bielen R,Buscchots D,Hens N,Nevens F,Robaeys G

    更新日期:2019-08-01 00:00:00

  • Enterovirus infection in children attending two outpatient clinics in Zhejiang Province, China.

    abstract::Enteroviruses are responsible for hand, foot, and mouth disease, and have caused many deaths in China during recent years. But the natural history of enterovirus infection in children, especially asymptomatic children, is not yet clear. From April 2011 to May 2012, 505 stool and throat swab samples of children attendi...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23884

    authors: Cai J,Lv H,Lin J,Chen Z,Fang C,Han J

    更新日期:2014-09-01 00:00:00

  • Analysis of the IgM and IgG antibody response against herpes simplex virus type 1 (HSV-1) structural and nonstructural proteins.

    abstract::In the present study the reactivity of IgG and IgM antibodies against HSV-1 structural and nonstructural proteins was analyzed by Western blot analysis (WBA) and radioimmunoprecipitation followed by polyacrylamide gel electrophoresis (RIPA-PAGE). It was demonstrated that IgM and IgG antibodies were directed against vi...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890230206

    authors: Kühn JE,Dunkler G,Munk K,Braun RW

    更新日期:1987-10-01 00:00:00

  • Detection of viruses in weekly stool specimens collected during the first 2 years of life: A pilot study of five healthy Australian infants in the rotavirus vaccine era.

    abstract::Several viruses are associated with gastroenteritis in infants. This pilot study, nested within a larger community-based project of early childhood infections, collected daily symptom data and 511 weekly stool samples from five healthy, fully vaccinated, term infants from birth until their second birthday. Real-time P...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24716

    authors: Ye S,Whiley DM,Ware RS,Sloots TP,Kirkwood CD,Grimwood K,Lambert SB

    更新日期:2017-05-01 00:00:00

  • Prevalence, genetic drift of haemagglutinin, and antiviral resistance of influenza A/H3N2 viruses circulating in Shanghai in children during 2009-2012.

    abstract::Influenza A/H3N2 viruses are associated with severe epidemics. Antiviral resistance and continued antigenic drift are the major concerns regarding prophylaxis and treatment of influenza. The objectives of this study were to investigate the prevalence and frequency of antiviral drug resistance in influenza A/H3N2 virus...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23854

    authors: Cai J,Wang X,Zhao B,Yao W,Wang X,Zhu Q,Zeng M

    更新日期:2014-06-01 00:00:00

  • Hemorrhagic fever with renal syndrome caused by Hantaan virus infection in four pregnant Chinese women.

    abstract::Hantavirus infection during pregnancy can influence both maternal and fetal outcomes. Here, we describe four cases of hemorrhagic fever with renal syndrome (HFRS) in pregnant Chinese women. The HFRS put these women at increased risk for severe illness, despite the patients' symptomologies in the onset phase were simil...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24856

    authors: Ji F,Zhao W,Liu H,Zheng H,Wang S,He C,Wang W,Zhang R,Bai D,Tian C,Zhao W,Deng H

    更新日期:2017-10-01 00:00:00

  • Weak correlation between antibody titers and neutralizing activity in sera from SARS-CoV-2 infected subjects.

    abstract::Plenty of serologic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been developed so far, thus documenting the importance of evaluating the relevant features of the immune response to this viral agent. The performance of these assays is currently under investigation. Amongst them, LIAISON®...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.26605

    authors: Criscuolo E,Diotti RA,Strollo M,Rolla S,Ambrosi A,Locatelli M,Burioni R,Mancini N,Clementi M,Clementi N

    更新日期:2020-10-16 00:00:00

  • Polymerase chain reaction assay for hepatitis C virus RNA using a single tube for reverse transcription and serial rounds of amplification with nested primer pairs.

    abstract::A procedure is described for the detection of hepatitis C virus (HCV) RNA in blood by means of the polymerase chain reaction (PCR) in which the reverse transcription step and two rounds of amplification are carried out in a single tube. This results in fewer manipulations, reduced risk of contamination, and economy of...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890380312

    authors: Lin HJ,Shi N,Mizokami M,Hollinger FB

    更新日期:1992-11-01 00:00:00

  • Prevalence of HBV genotypes in Central and Eastern Europe.

    abstract::The importance of hepatitis B virus (HBV) genotypes for disease progression and response to interferon-alpha-based treatment is well established. While almost all patients in the Mediterranean area are infected with HBV genotype D, HBV genotype A is dominant in Northern Europe. However, the distribution of HBV genotyp...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/jmv.21294

    authors: Deterding K,Constantinescu I,Nedelcu FD,Gervain J,Nemecek V,Srtunecky O,Vince A,Grgurevic I,Bielawski KP,Zalewska M,Bock T,Ambrozaitis A,Stanczak J,Takács M,Chulanov V,Slusarczyk J,Drazd'áková M,Wiegand J,Cornberg M,

    更新日期:2008-10-01 00:00:00

  • Association between HLA-DRB1 polymorphism, high-risk HPV infection and cervical neoplasia in southern Chinese.

    abstract::Multiple determinants are involved in the progression of human papillomavirus (HPV)-infected cervical lesion to invasive cancer. Human leukocyte antigen (HLA) polymorphism seems to play a role. This study examined the association between HLA-DRB1 polymorphism, high-risk HPV infection, and the development of cervical n...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20805

    authors: Chan PK,Cheung TH,Lin CK,Siu SS,Yim SF,Lo KW,Cheung JL,Tam AO,Tang JW

    更新日期:2007-07-01 00:00:00

  • Seroprevalence of markers of viral hepatitis in Yemeni healthcare workers.

    abstract::The seroprevalence of viral hepatitis in healthcare workers has important public health implications. To assess the risk factors for the acquisition of viral hepatitis in an unvaccinated cohort from an hyperendemic region, 567 healthcare workers from a large hospital in the capital of the Republic of Yemen were interv...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20126

    authors: Shidrawi R,Ali Al-Huraibi M,Ahmad Al-Haimi M,Dayton R,Murray-Lyon IM

    更新日期:2004-08-01 00:00:00

  • Infection with GB virus C/hepatitis G virus among blood donors and hemophiliacs in Martinique, a Caribbean island.

    abstract::GB virus C/hepatitis G virus (GBV-C/HGV) RNA was detected by reverse transcription-polymer- ase chain reaction with primers derived from the nonstructural region 3 (NS3) in 9 (4.1%) of 221 blood donors and 2 of 20 (10%) hemophilia patients in Martinique, French West Indies. Anti-E2 antibodies were found in sera from 3...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Césaire R,Martial J,Maier H,Kerob-Bauchet B,Bera O,Duchaud E,Brebion A,Pierre-Louis S

    更新日期:1999-10-01 00:00:00

  • Trends in HIV prevalence among drug-users attending public drug-treatment centres in Italy: 1990-2000.

    abstract::The occurrence of HIV infection among drug-users in Italy between 1990 and 2000 is described; the data concern drug-users attending a nation-wide network consisting of 510 public drug-treatment centres. We included only individuals with written documentation of an HIV-test result. Of the 1,299,972 attendees, 54.5% had...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20054

    authors: Suligoi B,Magliochetti N,Nicoletti G,Pezzotti P,Rezza G

    更新日期:2004-05-01 00:00:00