Abstract:
:In the present study the reactivity of IgG and IgM antibodies against HSV-1 structural and nonstructural proteins was analyzed by Western blot analysis (WBA) and radioimmunoprecipitation followed by polyacrylamide gel electrophoresis (RIPA-PAGE). It was demonstrated that IgM and IgG antibodies were directed against viral immediate-early, early, and late proteins. Following acute primary HSV infection, the early IgM antibody response in general was found to be directed against nonglycosylated structural proteins, viral early and immediate-early polypeptides. IgM antibodies against viral glycoproteins were found inconsistently. IgG antibodies against viral glycoproteins and other structural proteins with an apparent molecular weight of 56 kD, 45 kD, and 39 kD could be detected early in infection. Viral early and immediate-early proteins were poorly recognized by IgG antibodies in acute primary infections. In recurrent HSV infections, IgM antibodies revealed a less complex reaction with viral polypeptides. Thus, such IgM antibodies reacted predominantly with viral nonglycosylated structural proteins. In contrast, IgG antibodies from patients with recurrent infections strongly recognized viral structural, early, and immediate-early proteins. In seropositive individuals without obvious symptoms of acute infection, the most prominent antibody response was directed against gB and gD.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Kühn JE,Dunkler G,Munk K,Braun RWdoi
10.1002/jmv.1890230206subject
Has Abstractpub_date
1987-10-01 00:00:00pages
135-50issue
2eissn
0146-6615issn
1096-9071journal_volume
23pub_type
杂志文章abstract::Total RNA from normal adult woodchucks was analyzed using membrane arrays containing human cDNA clones, and the gene expression patterns were compared to human liver. Various hybridization and wash conditions were examined. In both the woodchuck and human livers, 352 genes were identified as highly expressed (Z-scores...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20186
更新日期:2004-10-01 00:00:00
abstract::Glycoproteins of enveloped viruses replicating in nonprimate mammalian cells carry α-1,3-galactose (α-Gal) glycans, and can bind to anti-Gal antibodies which are abundant in humans. The antibodies have protected humans and their ancestors for millions of years, because they inhibit replication of many kinds of microbe...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26312
更新日期:2020-07-18 00:00:00
abstract::To recognize the period of exaggerated cytokine response in patients with coronavirus disease 2019 (COVID-19) pneumonia, and to describe the clinical outcomes of using tocilizumab as a treatment option. The data of 12 adult COVID-19 pneumonia patients who were followed in the inpatient clinics of Biruni University Med...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26111
更新日期:2020-11-01 00:00:00
abstract::Interleukin 33 (IL-33) and soluble form of ST2 (sST2) are involved in inflammation. This study aimed to assess the role of serum IL-33 and sST2 in neonates with HCMV infection. The concentration of serum IL-33 and sST2 in 20 patients and 16 healthy controls were measure by enzyme-linked immune-sorbent assay (ELISA). T...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25187
更新日期:2018-08-01 00:00:00
abstract::Human papillomavirus (HPV) RNA levels may be a more sensitive early indicator of predisposition to carcinogenesis than DNA levels. We evaluated whether levels of HPV-16 and HPV-18 DNA and messenger RNA (mRNA) in newly detected infections are associated with cervical lesion development. Female university students were ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21450
更新日期:2009-04-01 00:00:00
abstract::Nineteen Chinese patients with chronic hepatitis B virus (HBV) infection, seropositive for HBV e antigen (HBeAg) and HBV DNA on at least three occasions in 6 months, were randomised to receive either recombinant human interferon-gamma (rIFN gamma) 0.1 mg/m2 intramuscularly thrice weekly for 16 weeks (n = 11) or no ant...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/jmv.1890340310
更新日期:1991-07-01 00:00:00
abstract::Knowledge of the antigenic diversity of rotaviruses circulating in a region should be acquired before introducing a rotavirus vaccine. In a collection of 151 rotavirus-positive samples from Mendoza, Argentina, strain diversity was evaluated utilizing G-typing monoclonal antibodies (MAbs), reverse-transcriptase-polymer...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::Herpes simplex virus can be quantitatively recovered from vaginal tampons suspended in tissue culture medium and stored over a wide range of temperatures. Because herpes simplex virus is stable for several days with refrigeration or after freezing, this method of culture of cervicovaginal virus lends itself to epidemi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890030306
更新日期:1979-01-01 00:00:00
abstract::The nature of the immunodominant a region of hepatitis B virus surface antigen (HBsAg) has been examined by mutation of specific amino acid residues. Proline 142 was required for the exhibition of full antigenicity. Replacement of cysteines 124 and 147 by serines drastically reduced or eliminated reactivity with antib...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890290310
更新日期:1989-11-01 00:00:00
abstract::Human hepatitis B virus (HBV) and hepatitis C virus (HCV) are two major etiologic agents of chronic hepatitis, which is closely related to the development of hepatocellular carcinoma (HCC). A possible involvement of HBV co-infection was investigated in ongoing HCV-related liver diseases in HCV-infected patients. A pre...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::The avalanche of genomic data generated from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus requires the development of tools to detect and monitor its mutations across the world. Here, we present a webtool, coronapp, dedicated to easily processing user-provided SARS-CoV-2 genomic sequences and...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26678
更新日期:2020-11-18 00:00:00
abstract::We developed an immunoperoxidase staining test to detect structural antigens of BK virus (BKV) in Vero cell cultures. This test was used to examine the neutralizing activity of human and immunized animal sera. It was shown that sera positive for BKV antibodies measured by hemagglutination inhibition test and enzyme-li...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890190311
更新日期:1986-07-01 00:00:00
abstract::Among 39,656 voluntary blood donors in Okinawa Prefecture, Japan, 115 (0.29%) were repeatedly reactive for antibody to hepatitis C virus (anti-HCV) by second generation (2nd-gen) passive hemagglutination assay (PHA). Positive serum samples were tested for anti-HCV using three different enzyme immunosorbent assays (ELI...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460408
更新日期:1995-08-01 00:00:00
abstract::A modified centrifugation culture technique and a polymerase chain reaction (PCR) is described for detection of early antigen and IE antigen DNA, respectively, for rapid and sensitive monitoring of active cytomegalovirus (HCMV) infection after organ transplantation. In a preliminary study, 541 clinical specimens (bloo...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890380404
更新日期:1992-12-01 00:00:00
abstract::Flow cytometric analysis of CD4(+) and CD8(+) T cells specific to human cytomegalovirus (CMV) was undertaken in seven patients with congenital CMV infection, six healthy infants who had acquired infection, and six CMV-seropositive adults. Intracellular cytokine assays showed that 0.03-2.23% of CD4(+) T cells in the he...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10477
更新日期:2003-10-01 00:00:00
abstract::The objectives of this study were to evaluate the reliability of herpes simplex virus (HSV) PCR testing in cerebrospinal fluid (CSF) for the detection of herpes simplex encephalitis. This was done by examining retrospectively the clinical follow-up of a large group of patients tested routinely by HSV-PCR. In addition,...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1082
更新日期:2001-08-01 00:00:00
abstract::Respiratory tract infections are a principal cause of illness and mortality in children worldwide and mostly caused by viruses. In this study, the epidemiology of 11 respiratory RNA viruses was investigated in a cohort of hospitalized children at a tertiary referral center in Riyadh from February 2008 to March 2009 us...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24435
更新日期:2016-06-01 00:00:00
abstract::Infectivity of certain enteric viruses including rotavirus is profoundly affected by proteolytic enzymes. To test whether cystic fibrosis patients, possessing chronically decreased levels of pancreatic enzymes, show altered susceptibility to gastroenteritis viruses, we examined sera from patients and controls for anti...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890090302
更新日期:1982-01-01 00:00:00
abstract::Noroviruses (NoVs) are a leading cause of acute gastroenteritis outbreaks and sporadic cases of diarrhea in industrialized countries. To study the prevalence and genetic diversity of NoVs in Guatemala, stool specimens were collected from hospitalized and ambulatory patients presenting with diarrhea (≥3 loose or liquid...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23578
更新日期:2013-07-01 00:00:00
abstract::Fifty-three (96%) of 55 patients with coronary artery stenosis were positive for serum anti-HHV-6 IgG, and 50 (91%) of these patients had anti-HHV-7 IgG. The number of cases sero-positive for HHV-6 and -7 in the 54 age matched control volunteers was 52 (96%) and 53 (98%), respectively. No statistical difference in the...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10134
更新日期:2002-08-01 00:00:00
abstract::Human papillomavirus (HPV) detection is used for screening of cervical cancer and genotype-specific persistence has shown to be mandatory for dysplasia development. Aim of this study was to evaluate the clinical performance of HPV DNA Array for cervical intraepithelial neoplasia 2+ (CIN2+) lesion detection. HPV DNA A...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25583
更新日期:2020-01-01 00:00:00
abstract::Primary infection, seroconversion, and transmitted drug resistance (TDR) during pregnancy may influence the risk of mother-to-child-transmission (MTCT) of HIV-1 infection. This study estimated recent seroconversion, TDR rates, HIV-1 subtypes and pregnancy outcomes among 95 recently diagnosed, antiretroviral (ARV)-naïv...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24540
更新日期:2016-11-01 00:00:00
abstract::Hemorrhagic fever with renal syndrome (HFRS) is a severe acute viral disease with pathological changes of impaired capillary and small vessels and thrombocytopenia. In this study, serum thrombospondin (TSP)-1 concentration in patients with HFRS was determined to explore its possible role in the pathogenesis of HFRS. T...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21270
更新日期:2008-10-01 00:00:00
abstract::The relationship of pre-bone-marrow transplant recipient and donor cytomegalovirus (CMV) antibody level to post-transplant recipient CMV excretion was investigated. CMV excretion was found to be related to pre-transplant recipient serological status, but not to level of antibody. This therefore contrasts with herpes s...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890330409
更新日期:1991-04-01 00:00:00
abstract::Mouse mammary tumor virus (MMTV) is a hormonally regulated, oncogenic virus of mice. MMTV-like virus DNA has previously been detected in human breast cancers, liver disease, and liver cancers. It is hypothesized that local hormonal effects might be of primary importance in determining MMTV-like virus detection in huma...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21754
更新日期:2010-05-01 00:00:00
abstract::Human astroviruses (HAstVs) have been recognized as one of the major causes of acute gastroenteritis in children. To provide more insight into the prevalence of HAstV gastroenteritis in China, 664 fecal samples were collected from children affected with acute gastroenteritis in Beijing from March 2005 to November 2007...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21729
更新日期:2010-03-01 00:00:00
abstract::We report a real-life 3D therapy failure in a patient treated with ombitasvir (OMV)/paritaprevir/ritonavir and dasabuvir without ribavirin (3D-R). He had therapy failure at week 12 after the end of treatment. We detected resistance-associated substitutions (RASs) plus polymorphisms on NS3, NS5A, and NS5B target region...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25073
更新日期:2018-07-01 00:00:00
abstract::The infection of enterovirus 71 (EV71) resulted in hand, foot, and mouth disease and may lead to severe nervous system damage and even fatalities. There are no effective drugs to treat the EV71 virus and it is crucial to find novel drugs against it. Polysaccharide isolated from Durvillaea antarctica green algae has an...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26693
更新日期:2020-11-24 00:00:00
abstract::Human parainfluenza virus 3 (HPIV-3) is a leading cause of respiratory disease in children worldwide. Previous sequence analyses of the entire virus genome, among different HPIV-3 strains, demonstrated that HN is the most variable gene. There is a dearth of data on HPIV-3 strains circulating in Saudi Arabia. In this r...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23326
更新日期:2012-08-01 00:00:00
abstract::Cytomegalovirus (CMV) is the most common congenital infection in humans and a leading cause of sensorineural hearing loss. Ganciclovir (6 mg/kg twice daily for 42 days) has been shown to reduce hearing deterioration and is used in clinical practice. Vaccines and passive administration of antibody are being evaluated i...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24654
更新日期:2017-03-01 00:00:00