Abstract：
:The infection of enterovirus 71 (EV71) resulted in hand, foot, and mouth disease and may lead to severe nervous system damage and even fatalities. There are no effective drugs to treat the EV71 virus and it is crucial to find novel drugs against it. Polysaccharide isolated from Durvillaea antarctica green algae has an antiviral effect. In this study, D. antarctica polysaccharide (DAPP) inhibited the infection of EV71 was demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), reverse transcription polymerase chain reaction, flow cytometry, and western blot. MTT assay showed that DAPP had no toxicity on Vero cells at the concentration 250 μg/ml. Furthermore, DAPP significantly reduced the RNA level of EV71 in a dose-dependent manner. Moreover, DAPP inhibited the Vero cells apoptosis induced by EV71 via the P53 signaling pathway. Meanwhile, the expression of signal transducer and activator of transcription 1 and mammalian target of rapamycin were increased and the proinflammatory cytokines were significantly inhibited by DAPP. Taken together, these results suggested that DAPP could be a potential pharmaceutical against the infection of EV71 virus.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Xu T,Li Y,Wu HL,Chen H,Wu H,Guo M,Zhao M,Wang C,Lin T,Lin Z,Chen D,Xiang W,Zhu Bdoi
10.1002/jmv.26693subject
Has Abstractpub_date
2020-11-24 00:00:00eissn
0146-6615issn
1096-9071pub_type
杂志文章abstract：:Immunocompromised individuals were tested for the presence of the human polyomaviruses JC (JCV) and BK (BKV) by the polymerase chain reaction (PCR). The use of appropriate primers in a nested PCR allowed the detection of both viruses simultaneously. Viruses were differentiated by restriction fragment length analysis o...
journal_title：Journal of medical virology
pub_type： 杂志文章
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journal_title：Journal of medical virology
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journal_title：Journal of medical virology
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journal_title：Journal of medical virology
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doi：
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journal_title：Journal of medical virology
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pub_type： 杂志文章
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journal_title：Journal of medical virology
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
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journal_title：Journal of medical virology
pub_type： 杂志文章,评审
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更新日期：2020-11-01 00:00:00
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journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/(sici)1096-9071(199711)53:3<306::aid-jmv21
更新日期：1997-11-01 00:00:00
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journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.1890200103
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pub_type： 杂志文章
doi：10.1002/jmv.1890130112
更新日期：1984-01-01 00:00:00
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journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.24112
更新日期：2015-04-01 00:00:00
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journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.23240
更新日期：2012-07-01 00:00:00
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journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/1096-9071(200012)62:4<505::aid-jmv16>3.0.c
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journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.24755
更新日期：2017-07-01 00:00:00
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journal_title：Journal of medical virology
pub_type： 杂志文章
doi：
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pub_type： 杂志文章
doi：10.1002/jmv.21490
更新日期：2009-06-01 00:00:00
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journal_title：Journal of medical virology
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doi：10.1002/jmv.21996
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pub_type： 杂志文章
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journal_title：Journal of medical virology
pub_type： 杂志文章
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journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.20593
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journal_title：Journal of medical virology
pub_type： 杂志文章
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journal_title：Journal of medical virology
pub_type： 杂志文章
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更新日期：2017-08-01 00:00:00
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journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/(sici)1096-9071(199910)59:2<221::aid-jmv16
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