Diagnosis of Oropouche virus infection by RT-nested-PCR.

Abstract:

:Using the RT-PCR with primers that anneal to the 5' and the 3' extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT-nested-PCR is a useful tool for rapid diagnosis of Oropouche fever infections.

journal_name

J Med Virol

authors

Moreli ML,Aquino VH,Cruz AC,Figueiredo LT

doi

10.1002/jmv.2122

keywords:

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

139-42

issue

1

eissn

0146-6615

issn

1096-9071

pii

10.1002/jmv.2122

journal_volume

66

pub_type

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