Abstract:
:Using the RT-PCR with primers that anneal to the 5' and the 3' extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT-nested-PCR is a useful tool for rapid diagnosis of Oropouche fever infections.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Moreli ML,Aquino VH,Cruz AC,Figueiredo LTdoi
10.1002/jmv.2122keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
139-42issue
1eissn
0146-6615issn
1096-9071pii
10.1002/jmv.2122journal_volume
66pub_type
杂志文章abstract::The avalanche of genomic data generated from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus requires the development of tools to detect and monitor its mutations across the world. Here, we present a webtool, coronapp, dedicated to easily processing user-provided SARS-CoV-2 genomic sequences and...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26678
更新日期:2020-11-18 00:00:00
abstract::Group A rotaviruses are the main cause of acute gastroenteritis in children worldwide. The intermediate capsid protein VP6 encoded by segment 6 of the dsRNA genome is the major structural component of the virus and it is highly antigenic and immunogenic. VP6 is responsible for group and subgroup (SG) specificities, al...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21306
更新日期:2008-11-01 00:00:00
abstract::To investigate the intra-hepatic activation of the IFN system in patients affected by chronic HCV-infection in comparison with that observed in a non-infectious liver disease such as non-alcoholic steatohepatitis, we measured the liver steady state mRNA levels of interferon-alpha, interferon-beta and interferon-gamma ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10433
更新日期:2003-08-01 00:00:00
abstract::A highly sensitive two-step polymerase chain reaction (PCR) method was evaluated for detection of human immunodeficiency virus type 1 (HIV-1) DNA in clinical specimens. The product resulting from the first amplification reaction is used as the template for the second PCR with an internal (nested) primer pair. Even whe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890380304
更新日期:1992-11-01 00:00:00
abstract::To elucidate the role of host immune status in the evolution and complexity of hepatitis C virus (HCV) quasispecies, three chronic HCV-infected patients who underwent bone marrow transplantation (BMT) were studied. The three transplanted patients' sera were sampled at pre-BMT, 3 months after BMT, and 12 months after B...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199906)58:2<132::aid-jmv6>
更新日期:1999-06-01 00:00:00
abstract::The incidence of noroviral gastroenteritis has increased dramatically in recent years, and norovirus (NoV) genogroup II.4 (GII.4) is associated with outbreaks worldwide. The NoV genotypes and their clinical relevance in children hospitalized with acute gastroenteritis between 2006 and 2011 in northern Taiwan were eval...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23728
更新日期:2014-02-01 00:00:00
abstract::Human respiratory syncytial virus group A (RSV-A) was detected in symptomatic hospital attended children in Central Spain for a continuous time period, September 2010 to April 2015. In order to accurately describe the epidemiology of this virus, the genetic diversity of the complete G gene and the clinical manifestati...
journal_title:Journal of medical virology
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doi:10.1002/jmv.24703
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abstract::Human parvovirus B19 commonly infects children, causing erythema infectiosum (fifth disease). However, there is a significant adult population which has not been exposed to the virus and, consequently, does not have protective antibody. Recent reports have associated B19 infection during pregnancy with fetal death, al...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890230409
更新日期:1987-12-01 00:00:00
abstract::Serum samples from 87 patients with hepatocellular carcinoma (HCC) in the United States were tested for evidence of hepatitis C viral (HCV) infection using an immunoblot assay for antibodies to the hepatitis C virus and the polymerase chain reaction to detect HCV RNA. The findings with these assays were compared to th...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890430205
更新日期:1994-06-01 00:00:00
abstract::Interleukin 6 (IL-6) is a pleiotropic cytokine with pivotal functions in the regulation of the biological responses of several target cells, including hepatocytes. Previous studies have shown that serum IL-6 levels are increased in hepatitis B patients. However, the role of IL-6 in modulating the anti-hepatitis B viru...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25382
更新日期:2019-05-01 00:00:00
abstract::Self-limited involvement of the central nervous system (CNS) is a relatively common complication of primary infection with human herpesvirus six (HHV-6) in normal children. We describe an HIV-infected infant who developed fulminant encephalitis as a complication of HHV-6 infection. Immunohistochemical staining of CNS ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890450309
更新日期:1995-03-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 07_BC strain has caused serious outbreaks among injection drug users in Taiwan since 2004. The objective of this study was to conduct a molecular epidemiological study of HCV genotypes in intravenous drug users in Taiwan. Blood samples and ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21658
更新日期:2010-01-01 00:00:00
abstract::Studies have shown that swine HEV (sHEV) and rabbit HEV (rHEV) can experimentally infect rabbits and swine, respectively. However, no published data have documented isolating sHEV strains from rabbits in natural environment so far. To clarify the possibility of natural cross-species transmission of sHEV to rabbits, th...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24374
更新日期:2016-04-01 00:00:00
abstract::Hepatitis B virus (HBV) DNA may persist in the liver in the absence of serum HBV-DNA after a self-limited acute hepatitis B. This may also occur in patients with chronic hepatitis C virus (HCV) infection but its prevalence and its impact on liver histology is unknown. HBV-DNA was tested by polymerase chain reaction (P...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20073
更新日期:2004-06-01 00:00:00
abstract::Several studies have indicated that viral reactivations following allogeneic hematopoietic stem cell transplantation (allo-HSCT) are frequent, but viral reactivations after autologous HSCT (auto-HSCT) have not been investigated in detail. We performed multiplex polymerase chain reaction (PCR) assay to examine multiple...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24621
更新日期:2017-02-01 00:00:00
abstract::Primary infection, seroconversion, and transmitted drug resistance (TDR) during pregnancy may influence the risk of mother-to-child-transmission (MTCT) of HIV-1 infection. This study estimated recent seroconversion, TDR rates, HIV-1 subtypes and pregnancy outcomes among 95 recently diagnosed, antiretroviral (ARV)-naïv...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24540
更新日期:2016-11-01 00:00:00
abstract::It has been generally believed that the Epstein-Barr virus (EBV)-encoded small RNA 1 and 2 (EBER1 and EBER2) genes are conserved as two families that correlated with type 1 (B95-8) and type 2 (AG876 or P3HR-1) EBV strains. EBER polymorphism and its association with EBV-associated disease have not received much attenti...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21714
更新日期:2010-05-01 00:00:00
abstract::Serial serum samples from 16 Italian patients presenting with acute hepatitis C virus (HCV) infections (which progressed to chronic hepatitis in six) were screened for the non-organ-specific autoantibodies most frequently associated with autoimmune hepatitis (AIH), as well as for antibodies against the hepatic asialog...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890420113
更新日期:1994-01-01 00:00:00
abstract::There are contradictory reports regarding the effects of inhalation anesthetics on the immune system. Measurable immune responses have been studied in vitro, but little is known about the in vivo effects in the intact organism. We used an attenuated, non-neuroinvasive, nonlethal strain of the encephalitic West Nile vi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2184
更新日期:2002-04-01 00:00:00
abstract::Hepatitis E is endemic in India. It was recently noted that although all the Indian human hepatitis E virus (HEV) isolates (1976-2001) were placed in genotype I, the swine HEV recovered from western India (2000) belonged to genotype IV. This was in contrast to reports from the United States and Taiwan wherein both hum...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10301
更新日期:2003-03-01 00:00:00
abstract::BKPyV replication is a risk factor for the development of polyomavirus-associated nephropathy in kidney transplant recipients. Here, the case of a 42 years old Caucasian patient is described who developed a kidney allograft failure because of uncontrolled BKPyV replication 7 months after transplant despite a strong re...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25458
更新日期:2019-09-01 00:00:00
abstract::Hepatitis A virus (HAV) is usually transmitted by an oral-fecal route and is prevalent not only in developing countries but also in developed countries. In the present study, the phylogenetic characterization of the VP1/2A junction region (321 nucleotides) of China HAV isolates was examined. Anti-HAV IgM-positive seru...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22086
更新日期:2011-07-01 00:00:00
abstract::GB virus-B (GBV-B) is the virus most closely related to hepatitis C virus (HCV). Thus, we have used GBV-B infection of tamarins, which develop acute hepatitis following experimental infection, as a surrogate model to study protective immunity. As challenge virus, we first produced a GBV-B pool from an infected tamarin...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21013
更新日期:2008-01-01 00:00:00
abstract::Asymptomatic carriers have a major influence on the spreading of norovirus infections. The objective of this study was to examine the characteristics of patients and asymptomatic carriers affected by norovirus-related community gastroenteritis outbreaks. No significant difference between the two groups was observed in...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25079
更新日期:2018-12-01 00:00:00
abstract::To characterize cytotoxic T lymphocytes (CTLs) that appeared in circulation during lamivudine therapy, we analyzed HBV-specific CTLs using HLA-A24 tetramer and HBcAg-specific Th1 cells in patients receiving lamivudine therapy. Six patients (HLA-A24(+)) with chronic hepatitis B, six patients (HLA-A24(-)) with chronic h...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20194
更新日期:2004-11-01 00:00:00
abstract::An outbreak of an acute sporadic viral hepatitis occurring at three villages in the lower Shebli region of southern Somalia was studied. Sera were examined for antibodies to hepatitis E virus (HEV) antigen by a recently developed enzyme-linked immunosorbent assay. The prevalence of anti-HEV ranged from 77.8% to 94.0% ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890400309
更新日期:1993-07-01 00:00:00
abstract::The present study compared the efficacy of standard and extended durations of PEG-IFNα-2a monotherapy for treatment of HBeAg-negative Chinese patients with chronic hepatitis B. Patients were randomized to receive standard therapy (n = 38; 48 weeks treatment) or extended therapy (n = 42; 72 weeks treatment). Extended t...
journal_title:Journal of medical virology
pub_type: 杂志文章,随机对照试验
doi:10.1002/jmv.24013
更新日期:2014-10-01 00:00:00
abstract::Healthcare-associated infections with hepatitis C virus (HCV) hitherto have been observed mainly in hemodialysis settings as well as in hematology and oncology wards. In this communication, molecular and epidemiologic investigations to elucidate an HCV outbreak in an orthopedic ward are reported. One hundred and thirt...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21394
更新日期:2009-02-01 00:00:00
abstract::Ultra violet (UV) light-inactivated measles virus was used for the lymphocyte proliferation assay, where it caused consistently adult human peripheral blood monolymphocytes (PBML) proliferation which was not dependent on the measles virus complement fixation titre in the donor's sera. When the cord blood lymphocytes f...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460209
更新日期:1995-06-01 00:00:00
abstract::A solid-phase radioimmunoassay (RIA) for the detection of hepatitis B e antigen (HBeAg) and antibody (anti-HBe) was developed. The RIA was approximately 1,000-fold more sensitive than rheophoresis for HBeAg, and approximately 6,000-fold more sensitive than rheophoresis for anti-HBe. Generally, less than one-fifth of h...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890020202
更新日期:1978-01-01 00:00:00