Abstract:
:We developed approaches using antisense RNA to inhibit hepatitis C virus (HCV) RNA translation and HCV core protein expression. An HCV genotype 1b cDNA comprising nt 1-1321 or a fusion construct consisting of HCV (nt 1-584) and luciferase cDNAs were inserted downstream of T7 and CMV promoter sequences and used to generate HCV RNA target molecules. Such constructs will produce HCV core or HCV coreluciferase fusion proteins in vitro or within transfected cells. Seven different antisense RNA constructs were designed to target the highly conserved 5' region of HCV RNA at nt positions 1-402. For in vitro experiments, synthesized HCV RNA target sequences and antisense RNAs were mixed at various molar ratios and subsequently translated in a rabbit reticulocyte lysate system. In cell culture studies, the HCV core-luciferase fusion cDNA was co-transfected with antisense RNA-producing constructs into human hepatocellular carcinoma (HCC) cells. Luciferase activity in cell lysates was measured to determine quantitatively antiviral effects within the cell. It was found that translation of HCV RNAs was efficiently inhibited by antisense RNA in vitro. The specificity of this inhibition was confirmed using control target RNA sequences or nonrelevant antisense RNA constructs. Co-transfection studies demonstrated that antisense RNA inhibited HCV core-luciferase fusion protein expression by 41-57% in HuH-7 HCC cells. These studies indicate that antisense RNA will find viral target RNA sequences in HuH-7 cells and inhibit HCV RNA translation. More important, these studies have defined critical viral RNA target sequences susceptible to antisense inhibitory effects within the cell.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Wakita T,Moradpour D,Tokushihge K,Wands JRkeywords:
subject
Has Abstractpub_date
1999-03-01 00:00:00pages
217-22issue
3eissn
0146-6615issn
1096-9071pii
10.1002/(SICI)1096-9071(199903)57:3<217::AID-JMV1>journal_volume
57pub_type
杂志文章abstract::The current study aimed to investigate the diagnostic value of glycated albumin (GA), glycated hemoglobin (HbA1c), and a number of routine biomarkers as noninvasive indicators of liver fibrosis in patients with chronic hepatitis C (CHC). One hundred patients with CHC were subjected to full medical history and examinat...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26204
更新日期:2020-06-19 00:00:00
abstract:BACKGROUND AND AIM:A meta-analysis was performed to identify COVID-19 patients presenting with gastrointestinal (GI) symptoms during the first and second pandemic waves and investigate their association with the disease outcomes. METHODS:A systematic search in PubMed, Scopus, Web of Science, ScienceDirect, and EMBASE ...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26836
更新日期:2021-02-02 00:00:00
abstract::Human herpesvirus 6 (HHV-6), the etiologic agent of roseola in young children, has been reported to be detectable in the brain of many neurologically normal adults, although regional localization to plaques of multiple sclerosis has also been demonstrated. Large amounts of this virus were present in multifocal demyeli...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199707)52:3<301::aid-jmv11
更新日期:1997-07-01 00:00:00
abstract::Rapid PCR-based influenza tests are increasingly used as point-of-care diagnostics in hospitals and clinics. To our knowledge, no prior studies have described clinical outcomes with implementation of rapid PCR-based influenza tests in hospitalized adult inpatients. Electronic medical records were used to assess differ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24279
更新日期:2015-12-01 00:00:00
abstract::Rotavirus is a major cause of gastroenteritis in young children worldwide. There have been several recent reports concerning rotavirus isolation from adults, particularly in the elderly, presenting with gastroenteritis. In this study, the authors report on rotavirus outbreaks in five separate elderly care facilities b...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23416
更新日期:2012-12-01 00:00:00
abstract::An outbreak of dengue type 2 occurred in North Queensland, Australia, between December 1996 and April 1997. Culture of serum in the Aedes albopictus C6/36 cell line with detection using immunofluorescent staining was compared with a culture-amplified detection system using an immunoperoxidase staining method in a micr...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract::The occurrence of HIV infection among drug-users in Italy between 1990 and 2000 is described; the data concern drug-users attending a nation-wide network consisting of 510 public drug-treatment centres. We included only individuals with written documentation of an HIV-test result. Of the 1,299,972 attendees, 54.5% had...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20054
更新日期:2004-05-01 00:00:00
abstract::Human parechoviruses (HPeVs) infection is associated with a wide range of clinical syndromes such as respiratory, gastrointestinal, neurologic diseases, and neonatal sepsis-like illness. The main objective of this study was to investigate the epidemiology of HPeVs infection in hospitalized patients in a period of 2 ye...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23197
更新日期:2012-04-01 00:00:00
abstract::Hepatitis E virus (HEV) infection is a significant public health issue in many developing countries, causing waterborne outbreaks as well as sporadic hepatitis. We report here an outbreak of HEV genotype 1f infection during April-May 2018 among people living at Halisohor, a low land in the southern part of Chottogram ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26739
更新日期:2020-12-17 00:00:00
abstract::Molecular investigation was undertaken of circulating hepatitis A virus (HAV) associated with cases of acute diarrhea among children under 5 years of age in Kumba-Cameroon. Reverse transcription PCR, sequencing, and phylogenetic analysis of a 371 bp segment of the VP1/P2A junction of six isolates obtained from stool s...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23266
更新日期:2012-05-01 00:00:00
abstract::Limited data are available describing human papillomavirus (HPV) genotype distribution among females with cytological abnormalities in Switzerland. Cervical cell specimens obtained from 5,318 women were screened routinely by liquid-based Pap smear. All specimens with cellular abnormalities were analyzed subsequently f...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22126
更新日期:2011-08-01 00:00:00
abstract::ZIKV infection has become a global threat spreading across 31 countries in Central America, South America, and the Caribbean. However, little information is available about the molecular epidemiology of ZIKV. Shared mutation of a threonine residue to alanine at the same position in the C terminal of NS5 sequences was ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24615
更新日期:2016-10-01 00:00:00
abstract::Guinea pig infection with a highly passaged XJ prototype strain of Junín virus by the intramuscular route (IM) was carried out in order to study viral tropism modification in this host. The neurotropism of this strain was demonstrated by viral isolation, meningitis, and by the presence of Junín antigens as shown by im...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890150208
更新日期:1985-02-01 00:00:00
abstract::Sixty-seven rotavirus-positive fecal samples, collected between January and April 1999, from children with diarrhea in the Upper East Region of Ghana were examined for rotavirus VP7 and VP4 types. Sufficient viral RNA could be obtained from 46 (68.7%) of the samples and all the isolates had short electrophoretic patte...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Two hundred and thirty fecal specimens were collected from children (up to 5 years of age) admitted with suspected rotaviral gastroenteritis at four Irish hospitals (Cork University Hospital, Mercy Hospital, Cork, Waterford Regional Hospital, and Kerry General Hospital) in the southern region of Ireland, between 2001 ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20494
更新日期:2005-12-01 00:00:00
abstract::Swabbing the surface of a genital lesion to obtain a sample for HPV DNA testing is less invasive than a biopsy, but may not represent HPV types present in the lesion tissue. The objective of this study was to examine the concordance of HPV types detected in swab and biopsy samples from 165 genital lesions from men age...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23635
更新日期:2013-09-01 00:00:00
abstract::During the period between 1998 and 2008, 48 representative measles viruses (MeVs) circulating in Thailand were subjected to genetic characterization. Three genotypes, G2, D5, and D9 were detected. The results suggested that measles genotype D5, which has been circulating since at least 1998, is the endemic genotype in...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23249
更新日期:2012-05-01 00:00:00
abstract::We read with great interest the recent issue,1 which raised the concern about the co-circulation of dengue during the COVID-19 pandemic. This article is protected by copyright. All rights reserved. ...
journal_title:Journal of medical virology
pub_type: 信件
doi:10.1002/jmv.26650
更新日期:2020-11-03 00:00:00
abstract::The nucleotide sequences of the precore/core and X open reading frames (ORFs) of hepatitis B virus (HBV) were studied in four subjects who were serologically negative for anti-hepatitis B core antibody. These subjects were positive for serum hepatitis B surface antigen and were considered to be asymptomatic HBV carrie...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460306
更新日期:1995-07-01 00:00:00
abstract::Enteric adenoviruses are important etiological agents associated with sporadic infections and outbreaks of acute gastroenteritis in infants and children. Fecal samples were collected from 439 hospitalized patients in the years 2005-2007 from Pune, Aurangabad, and Nagpur cities of western India to identify the most pre...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21331
更新日期:2009-01-01 00:00:00
abstract::This open, randomized study was conducted in healthy Chinese youngsters, aged between 10 and 19 years to compare the reactogenicity and immunogenicity of two vaccines: the combined vaccine against hepatitis A and B was administered in a two-dose schedule with the profile of the corresponding monovalent vaccines, while...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1999-09-01 00:00:00
abstract::The seroprevalence of viral hepatitis in healthcare workers has important public health implications. To assess the risk factors for the acquisition of viral hepatitis in an unvaccinated cohort from an hyperendemic region, 567 healthcare workers from a large hospital in the capital of the Republic of Yemen were interv...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20126
更新日期:2004-08-01 00:00:00
abstract::Sera from patients with sandfly fever caused by Toscana virus (TOSV) infection were tested by immunoblot for specific antibody response to TOSV derived from infected Vero-E6 cells. The 28 kDa TOSV nucleoprotein (N) was identified as the major immunodominant protein recognized by immunoblot. In sera of patients with ac...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9071(199606)49:2<83::AID-JMV2>3
更新日期:1996-06-01 00:00:00
abstract::Puumala hantavirus (PUUV) glycoproteins G1 and G2 and nucleocapsid protein (N) were expressed in BHK-21 cells by transfection of a plasmid producing a recombinant alphavirus replicon. Coexpression of G1 and G2 from separate constructs seemed to be important for the optimal folding of the glycoproteins, as evaluated by...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::Coronavirus disease-2019 (COVID-19) infection and its severity can be explained by the concentration of glycosylated severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) viral particles in the lung epithelium, the concentration of glycosylated angiotensin-converting enzyme receptor 2 (ACE2) in the lung epithel...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.25887
更新日期:2020-07-01 00:00:00
abstract::Human respiratory syncytial virus group A (RSV-A) was detected in symptomatic hospital attended children in Central Spain for a continuous time period, September 2010 to April 2015. In order to accurately describe the epidemiology of this virus, the genetic diversity of the complete G gene and the clinical manifestati...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24703
更新日期:2017-05-01 00:00:00
abstract::Infection with putative non-A to E hepatitis virus, designated GB virus C (GBV-C), was surveyed in 286 patients with chronic liver disease in Japan. RNA of GBV-C was detected, by reverse-transcription polymerase chain reaction with nested primers from the 5'-noncoding region, in 19 patients (6.6%) at a frequency highe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::A new assay was developed for the detection of hepatitis B virus (HBV) in human serum using amplification of a short viral DNA sequence by means of the polymerase chain reaction. As little as 0.4 fg viral DNA, corresponding to about 130 genome equivalents, per ml serum could be detected after the amplification procedu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890270409
更新日期:1989-04-01 00:00:00
abstract::Hepatitis E is endemic in India. It was recently noted that although all the Indian human hepatitis E virus (HEV) isolates (1976-2001) were placed in genotype I, the swine HEV recovered from western India (2000) belonged to genotype IV. This was in contrast to reports from the United States and Taiwan wherein both hum...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10301
更新日期:2003-03-01 00:00:00
abstract::Few studies have examined the etiology of hepatocellular carcinoma (HCC) in patients without hepatitis virus infection. We evaluated the role of occult hepatitis B virus (HBV) infection in the development of HCC in Japanese patients without hepatitis B surface antigen (HBsAg) and antibodies to hepatitis C antigen (ant...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10514
更新日期:2003-12-01 00:00:00