Abstract:
:An outbreak of dengue type 2 occurred in North Queensland, Australia, between December 1996 and April 1997. Culture of serum in the Aedes albopictus C6/36 cell line with detection using immunofluorescent staining was compared with a culture-amplified detection system using an immunoperoxidase staining method in a microtiter plate format. A total of 374 serum specimens from individuals during the outbreak were tested. Ninety-five specimens were positive using immunofluorescence and ninety-two were positive using the immunoperoxidase method (sensitivity 91.6%; specificity 98.2%). The immunoperoxidase method is quicker, easier to perform, and does not require the use of an immunofluorescent microscope. The method is more suited to the processing of large numbers of specimens in an outbreak and could be used in endemic areas with limited virological resources.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Gleeson F,McBride J,Norton Rsubject
Has Abstractpub_date
1999-02-01 00:00:00pages
212-5issue
2eissn
0146-6615issn
1096-9071pii
10.1002/(SICI)1096-9071(199902)57:2<212::AID-JMV21journal_volume
57pub_type
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