Abstract:
:Rabbit antibodies produced by immunization with complete hepatitis A virions (HAV) recognized all the viral structural proteins and neutralized HAV infectivity in cell culture. Rabbit antibodies to chromatographically purified individual viral proteins and to synthetic peptides representing epitopes on the structural viral protein VP1 neither recognized whole virus nor neutralized infectivity, indicating that native epitopes on the virus surface are necessary for virus recognition and neutralization. Human anti-HAV-positive sera of the acute and convalescent phase of disease recognized and neutralized viral particles. Analysis of the immunoreactivity of these human sera in immunoblot showed that the IgM antibody preferentially recognizes the structural viral proteins VP0 and VP3 of HAV, whereas IgA and IgG antibodies reacted more strongly with VP1.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Gauss-Müller V,Deinhardt Fdoi
10.1002/jmv.1890240212subject
Has Abstractpub_date
1988-02-01 00:00:00pages
219-28issue
2eissn
0146-6615issn
1096-9071journal_volume
24pub_type
杂志文章abstract::The ability of the total hepatitis C virus (HCV) core antigen assay was evaluated for monitoring the therapeutic responses of HCV-infected patients treated with interferon. The ability to detect and quantitate an independent structural protein component of HCV, in the presence of circulating antibodies, makes this ass...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10355
更新日期:2003-05-01 00:00:00
abstract::In this study, real-time PCR was used to quantify adenovirus DNA in cell suspensions prepared from 106 right and left tonsils and 10 adenoids obtained from 57 patients who underwent routine tonsillectomies and/or adenoidectomies. Eighty-four (72.4%) tonsils and adenoids samples were positive for HAdV by real-time PCR....
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23678
更新日期:2013-11-01 00:00:00
abstract::Polymorphisms upstream interleukin (IL)-28B gene and serum levels of interferon gamma inducible protein-10 (IP-10) are associated with spontaneous and treatment-induced hepatitis C virus (HCV) clearance. Patients with seronegative occult HCV infection are anti-HCV and serum HCV-RNA negative but have viral RNA in liver...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24322
更新日期:2016-02-01 00:00:00
abstract::The seroprevalence of antibodies to hepatitis E virus (HEV) and hepatitis A virus (HAV) was determined in a community-based sample in innercity London where socioeconomic conditions were expected to result in a high prevalence of antibodies to HAV, and in which the presence of immigrants from the developing world pose...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9071(199607)49:3<230::AID-JMV12
更新日期:1996-07-01 00:00:00
abstract::Human herpes virus 8 (HHV 8) is recognized as the necessary cause of Kaposi sarcoma (KS) and in the recent past the human papillomavirus (HPV) has been linked to the development of cutaneous basal cell and squamous cell carcinomas. In a cross sectional study investigating Beta-HPV infections in skin lesions, an unexpe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23944
更新日期:2014-09-01 00:00:00
abstract::Most dengue virus (DENV) infections remain asymptomatic. This increases the risk of DENV transfusion transmission (TT-DENV) during outbreaks. We evaluated DENV viremia in 8475 blood donations assembled in minipools for the presence of DENV RNA. The tested samples were obtained between February and May, 2016, during a ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26619
更新日期:2020-10-22 00:00:00
abstract::A cohort of 127 nursing home residents aged 60-98 years were vaccinated during the winter of 1985-86 with the A-Chile 1/83 (C), A-Philippines 2/82 (P), and B-USSR (B) commercial influenza vaccines. Before vaccination 40%, 23%, and 69% were susceptible to influenza Ac, Ap, and B, respectively [hemagglutinin inhibition ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890250112
更新日期:1988-05-01 00:00:00
abstract::Sixty-seven rotavirus-positive fecal samples, collected between January and April 1999, from children with diarrhea in the Upper East Region of Ghana were examined for rotavirus VP7 and VP4 types. Sufficient viral RNA could be obtained from 46 (68.7%) of the samples and all the isolates had short electrophoretic patte...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::We have previously reported that concanavalin A-immobilized polystyrene nanospheres (Con A-NS) could efficiently capture HIV-1 particles and that intranasal immunization with inactivated HIV-1-capturing nanospheres (HIV-NS) induced vaginal anti-HIV-1 IgA antibody response in mice. In this study, to evaluate the protec...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20100
更新日期:2004-07-01 00:00:00
abstract::A line of hepatitis C virus (HCV) transgenic mice was established previously that was mediated by Cre/loxP system using HCV cDNA, including core, E1, E2 and NS2 genes. Intravenous infection of a recombinant adenovirus that expresses Cre DNA recombinase (AxCANCre) induced HCV structural protein expression in the liver ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/1096-9071(200011)62:3<308::aid-jmv2>3.0.co
更新日期:2000-11-01 00:00:00
abstract::Systemic cell-mediated responses to respiratory syncytial (RS) virus were detected, using a whole blood transformation assay, in 10 of 28 infants and children with RS virus infections during the period 1-14 days postadmission. Cell-mediated responses were unrelated to the age of the patient or the severity of illness....
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890130112
更新日期:1984-01-01 00:00:00
abstract::Individuals infected with HIV have higher circulating Epstein-Barr virus (EBV) DNA load compared to healthy carriers. This study investigated whether level of spontaneous immunoglobulin secreting cells, one of the major hallmarks of HIV infection, is associated with an increase of EBV DNA load in PBMCs and the spontan...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23762
更新日期:2014-01-01 00:00:00
abstract::This study aimed at developing strategies for screening, predicting, and diagnosing intrauterine HBV infection in infants born to HBsAg positive mothers. A total of 1,360 infants born to 1,355 HBsAg positive mothers were followed for 1 year. All newborn infants received active and passive immunization within 24 hr aft...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23667
更新日期:2013-10-01 00:00:00
abstract::The ability of hepatitis A virus (HAV) to agglutinate human erythrocytes was used to develop IgM and IgG antibody capture haemadherence tests (MACHAT and GACHAT). Haemadherence was dependent on the pH of the red cell suspension and was best in the pH range 5.4 to 5.8. The tests were applied to serum, urine, and saliva...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890390106
更新日期:1993-01-01 00:00:00
abstract::It was reported previously that HCV can be transmitted from persistently infected human bone-marrow-derived B-lymphoblastoid cells (TO.FE(HCV)) to human hepatoma cells by cell-to-cell contact. The present study confirms and characterize further such type of HCV infection in vitro. TO.FE(HCV) cells were co-cultured wit...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20947
更新日期:2007-10-01 00:00:00
abstract::Rotavirus is a major cause of gastroenteritis in young children worldwide. There have been several recent reports concerning rotavirus isolation from adults, particularly in the elderly, presenting with gastroenteritis. In this study, the authors report on rotavirus outbreaks in five separate elderly care facilities b...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23416
更新日期:2012-12-01 00:00:00
abstract::Genital herpes is a common, distressing infection whose incidence has been underestimated. Recent serological surveys, employing type-specific antibody assays, show a rising prevalence of previous herpes simplex virus (HSV) type 2 infections in post-adolescent populations in developed countries; many of these infectio...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.1890410508
更新日期:1993-01-01 00:00:00
abstract::Human metapneumovirus (hMPV) has been described as circulating among the Uruguayan population at least since 1998 based on serologic evidence. However, no isolation attempts, molecular detection, or genetic studies have been carried out so far in the country. In the present study, molecular detection of circulating hM...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21752
更新日期:2010-05-01 00:00:00
abstract::A viable virus could not be recovered from a mutant murine cytomegalovirus (MCMV) BAC in which the M34 ORF had been deleted (BACDeltaM34). In contrast, an M34 mutant virus (RcM34), in which the M34 ORF was interrupted by transposon insertion at nt 44,827 of the Smith MCMV BAC, was attenuated in replication both in tis...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21211
更新日期:2008-07-01 00:00:00
abstract::A quantitative polymerase chain reaction (PCR) assay for hepatitis C viral RNA (HCV-RNA) was used to monitor viraemia levels in six patients at multiple time points before, during, and after interferon therapy for chronic non-A, non-B hepatitis (NANBH). Prior to therapy, serum HCV-RNA was detected in all patients at a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890370311
更新日期:1992-07-01 00:00:00
abstract::Variation at the IL-28B locus was recently reported to be a significant predictive factor of viral response to pegylated-interferon plus ribavirin combination therapy against chronic hepatitis C. Predictive factors for the effect of therapy, including IL-28B polymorphism rs8099917 and viral and clinical factors were i...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22028
更新日期:2011-06-01 00:00:00
abstract::The reported positive rates of hepatitis B virus surface antigen (HBsAg) among Chinese blood donors generally do not include data from pre-donation rapid tests. The aim of this study was to estimate the prevalence of serologic markers for HBsAg and risk factors among blood donors from Chengdu, China. From April 2010 t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24339
更新日期:2016-02-01 00:00:00
abstract::The human parvovirus B19 (B19) usually causes a subclinical infection in immunocompetent individuals. Whereas immunocompromised individuals such as patients infected with HIV are at risk of persistent anemia due to B19 infection. Only few studies have been carried out on distribution and molecular epidemiology of B19 ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24169
更新日期:2015-07-01 00:00:00
abstract::The risk and clearance of GB virus type C (GBV-C)/hepatitis G virus (HGV) infection was investigated in a cohort of homosexual men (n=180; median follow-up time, 7 years). The interaction between GBV-C/HGV RNA and antibodies against the E2 region of the virus, and the clinical impact of chronic GBV-C/HGV infection wer...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199911)59:3<303::aid-jmv7>
更新日期:1999-11-01 00:00:00
abstract::To investigate the intra-hepatic activation of the IFN system in patients affected by chronic HCV-infection in comparison with that observed in a non-infectious liver disease such as non-alcoholic steatohepatitis, we measured the liver steady state mRNA levels of interferon-alpha, interferon-beta and interferon-gamma ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10433
更新日期:2003-08-01 00:00:00
abstract::To characterize the state of dual infection with hepatitis B virus (HBV) and hepatitis C virus (HCV), HBV-DNA and HCV-RNA levels were determined by spot hybridization or polymerase chain reaction in the sera of patients who were positive for both hepatitis B surface antigen and HCV antibody. Among 27 patients who show...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890450222
更新日期:1995-02-01 00:00:00
abstract::Hepatitis E virus (HEV) infection is a significant public health issue in many developing countries, causing waterborne outbreaks as well as sporadic hepatitis. We report here an outbreak of HEV genotype 1f infection during April-May 2018 among people living at Halisohor, a low land in the southern part of Chottogram ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26739
更新日期:2020-12-17 00:00:00
abstract::The ability to detect type-specific high risk HPV (HR-HPV) infections in samples from females and males is important for monitoring the epidemiology of HPV and the impact of vaccination. Type-specific detection concordance between paired urine and genital samples from females (n = 264) undergoing routine colposcopy an...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22167
更新日期:2011-10-01 00:00:00
abstract::The quantitation of serum levels of hepatitis C virus (HCV) RNA in chronic hepatitis C has been regarded as one of the most important indicators for the outcome of interferon (IFN) therapy. The AMPLICOR HCV MONITOR version 1.0 (AMPLICOR v1.0) assay is widely used for the evaluation of the HCV level. A new generation a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10209
更新日期:2002-11-01 00:00:00
abstract::Infectivity of certain enteric viruses including rotavirus is profoundly affected by proteolytic enzymes. To test whether cystic fibrosis patients, possessing chronically decreased levels of pancreatic enzymes, show altered susceptibility to gastroenteritis viruses, we examined sera from patients and controls for anti...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890090302
更新日期:1982-01-01 00:00:00