Abstract:
:Transmission of West Nile virus (WNV) from asymptomatic donors has been reported during blood transfusions and organ transplants in humans. In this work, we aimed to investigate the presence of WNV antibody and WNV RNA in blood donors to evaluate the sero-prevalence of WNV and risk for WNV transmission. One hundred and sixty blood donors were tested for the presence of anti-WNV IgG by ELISA and for WNVs 1 and 2 RNA by RT-PCR. About 55% of blood donors were seropositive for WNV IgG antibodies, with significantly higher percentage of positive donors coming from rural areas and Nile Delta region compared to other donors. Using RT-PCR all donors were negative for viral RNA of both WNV lineages 1 and 2. High sero-prevelance of WNV antibodies in asymptomatic blood donors denotes endemicity of the WNV in Egypt and points to the importance of routine screening of blood donors for WNV RNA. On the other hand the absence of WNV RNA by RT-PCR indicates apparent low risk of the blood products as regards WNV transmission. Further studies into significance of WNV seronegativity among Rh negative donors and into the use of WNV seropositive blood in prophylaxis or treatment of WNV neuroinvasive disease are recommended. J. Med. Virol. 89:1323-1329, 2017. © 2017 Wiley Periodicals, Inc.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Youssef SR,Eissa DG,Abo-Shady RA,Aly Fouad NT,Kattab DK,Fathey H,Abdullaha Elewa AA,Reda DMdoi
10.1002/jmv.24682subject
Has Abstractpub_date
2017-08-01 00:00:00pages
1323-1329issue
8eissn
0146-6615issn
1096-9071journal_volume
89pub_type
杂志文章abstract::In end-stage renal disease patients treated by hemodialysis with HBeAg-negative chronic hepatitis B virus (HBV) infection, the evaluation of the presence of viral replication is essential in the assessment for renal transplantation. Data on HBV viral load, prevalence of precore mutations, as well as the influence of H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20691
更新日期:2006-10-01 00:00:00
abstract::Present diagnostic criteria for occult hepatitis B virus (HBV) infection requires blood or liver samples to test positive for at least two HBV DNA targets in hepatitis B surface antigen (HBsAg) negative individuals. These criteria were derived from studies involving referred or selected patient populations. The object...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23813
更新日期:2014-01-01 00:00:00
abstract::Immunological factors, such as cytokines, have been proposed as a cause of changes in the lipid profile of dengue patients. We studied whether serum lipid levels and serum TNF-α levels are associated in a group of dengue patients from an endemic region in the Northwest of Mexico. We found statistically important diffe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25056
更新日期:2018-06-01 00:00:00
abstract::We designed experiments to assess the effects of binding of the HSV-2 Fc receptor (FcR) to purified rabbit nonimmune IgG and purified Fc. Purified Fc (75 micrograms) or nonimmune IgG (100 micrograms) when bound to HSV-2 did not reduce infectivity but did protect the virions against thermal inactivation at 37 degrees C...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890130307
更新日期:1984-01-01 00:00:00
abstract::GB virus C/hepatitis G virus (GBV-C/HGV) RNA was detected by reverse transcription-polymer- ase chain reaction with primers derived from the nonstructural region 3 (NS3) in 9 (4.1%) of 221 blood donors and 2 of 20 (10%) hemophilia patients in Martinique, French West Indies. Anti-E2 antibodies were found in sera from 3...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::The rapid emergence of coronavirus disease 2019 (COVID-19) has necessitated the implementation of diverse pandemic control strategies throughout the world. To effectively control the spread of this disease, it is essential that it be diagnosed at an early stage so that patients can be reliably quarantined such that di...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26081
更新日期:2020-11-01 00:00:00
abstract::Despite the success of antivirals in preventing clinically overt CMV disease in cardiac allograft recipients, sub-clinical active CMV infection remains a major concern because of its association with allograft rejection and vasculopathy. The measurement of CMV specific T-cell responses is a promising approach to asses...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21229
更新日期:2008-09-01 00:00:00
abstract::Faeces from 746 children less than 5 years old with acute gastroenteritis were screened for the presence of adenovirus particles or antigens by immunoelectron microscopy (IEM) and enzyme immunoassay (EIA). Thirty-five samples were positive by both IEM and EIA, two only by IEM, and two only by EIA, giving a total of 39...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890150213
更新日期:1985-02-01 00:00:00
abstract::The infection of enterovirus 71 (EV71) resulted in hand, foot, and mouth disease and may lead to severe nervous system damage and even fatalities. There are no effective drugs to treat the EV71 virus and it is crucial to find novel drugs against it. Polysaccharide isolated from Durvillaea antarctica green algae has an...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26693
更新日期:2020-11-24 00:00:00
abstract::The aim of the study was to assess whether high-risk pregnant women have a higher prevalence of HEV during the perinatal period. This was a cross-sectional study of 428 patients: Group 1, 127 women with a high-risk pregnancy; Group 2, 97 asymptomatic people with reactivity to HCV or HBV; Group 3, 94 patients with clin...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24878
更新日期:2017-11-01 00:00:00
abstract::Self-limited involvement of the central nervous system (CNS) is a relatively common complication of primary infection with human herpesvirus six (HHV-6) in normal children. We describe an HIV-infected infant who developed fulminant encephalitis as a complication of HHV-6 infection. Immunohistochemical staining of CNS ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890450309
更新日期:1995-03-01 00:00:00
abstract::Subviral particles of hepatitis B virus have been used to present foreign epitopes. We attempted to present the hydrophilic domains of E2 envelope protein of hepatitis C virus (HCV) as a fusion protein with hepatitis B virus surface antigen (HBsAg). The five hydrophilic domains of HCV E2 antigen were inserted into HBs...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9071(199610)50:2<145::AID-JMV7>
更新日期:1996-10-01 00:00:00
abstract::The emergence of genotypic resistance in protease and reverse transcriptase (RT) gene regions was longitudinally evaluated in plasma samples from a group of 12 HIV-1-infected patients treated with different combination of antiretroviral therapies and selected on the basis of their clinical failure. Complex mutational ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199912)59:4<480::aid-jmv10
更新日期:1999-12-01 00:00:00
abstract::The expression levels of many genes change after treatment of human immunodeficiency virus (HIV)-infected subjects by antiretroviral drugs. High-throughput analysis of tremendous datasets led to the discovery of genes that are implicated in the treatment pathways. In this study, we performed a gene-enrichment analysis...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25549
更新日期:2019-12-01 00:00:00
abstract::Rotavirus is a major cause of gastroenteritis in young children worldwide. There have been several recent reports concerning rotavirus isolation from adults, particularly in the elderly, presenting with gastroenteritis. In this study, the authors report on rotavirus outbreaks in five separate elderly care facilities b...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23416
更新日期:2012-12-01 00:00:00
abstract::Chronic papillary conjunctivitis has been described following adenoviral conjunctivitis. It is unknown however, how long adenovirus is able to persist in the tear film and conjunctiva. To determine if adenovirus persists in the ocular surface following adenoviral conjunctivitis, 304 patients with a history of adenovir...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20440
更新日期:2005-10-01 00:00:00
abstract::Human cytomegalovirus (HCMV) is the leading cause of congenital infection and an opportunistic pathogen capable of establishing lifelong latency. In the present study, we aimed to investigate the distribution of glycoprotein B, H, and N in infants of Shanghai and correlate the genotype with active and latent HCMV infe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26210
更新日期:2020-06-21 00:00:00
abstract::Chronic hepatitis B virus (HBV) infection is the result of an inadequate immune response towards the virus. Dendritic cells (DCs), as the most efficient professional antigen-presenting cells (APCs), possess the strongest antigen presenting the effect in the body and can stimulate the initial T cell activation and prol...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.24306
更新日期:2016-01-01 00:00:00
abstract::Chronic hepatitis C (HCV) infection is associated with cognitive impairments which might be mediated through a secondary inflammatory cascade. Egypt has an unusually high prevalence of HCV monoinfections and is an ideal site for the study of the isolated effects of HCV infection. Therefore, in a hospital-based cross-s...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21879
更新日期:2011-02-01 00:00:00
abstract::To evaluate the variation over time of seroreactivity to human papillomavirus type 11 (HPV-11) according to disease outcome, we selected a sample of 42 condyloma acuminatum patients from a group of subjects enrolled in a placebo-controlled trial of three alpha-interferon preparations administered parenterally for the ...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1002/jmv.1890390414
更新日期:1993-04-01 00:00:00
abstract::The occurrence of HIV infection among drug-users in Italy between 1990 and 2000 is described; the data concern drug-users attending a nation-wide network consisting of 510 public drug-treatment centres. We included only individuals with written documentation of an HIV-test result. Of the 1,299,972 attendees, 54.5% had...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20054
更新日期:2004-05-01 00:00:00
abstract::This study analyzed the genotype distribution and frequency of lamivudine (LAM) and tenofovir (TDF) resistance mutations in a group of patients co-infected with HIV and hepatitis B virus (HBV). A cross-sectional study of 847 patients with HIV was conducted. Patients provided blood samples for HBsAg detection. The load...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21845
更新日期:2010-09-01 00:00:00
abstract::There is an obvious concern globally regarding the fact about the emerging coronavirus 2019 novel coronavirus (2019-nCoV) as a worldwide public health threat. As the outbreak of COVID-19 causes by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) progresses within China and beyond, rapidly available epi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25743
更新日期:2020-06-01 00:00:00
abstract::Dried blood spots collected on filter paper are considered potential clinical specimens for measles surveillance because of their ease of collection, storage, and transport. The usefulness of these samples for surveillance of measles was evaluated in a field setting. Blood spots were collected by finger-prick from 316...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20136
更新日期:2004-08-01 00:00:00
abstract::From November 2004 to April 2007, specimens were obtained from 2,281 patients with acute respiratory tract illness in Normandy, France. Eighteen strains of influenza C virus were detected in these samples using a combined tissue culture/RT-PCR diagnostic method. Most patients with influenza C virus infection (13/18) w...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21218
更新日期:2008-08-01 00:00:00
abstract::The general aim was to investigate the burden of respiratory virus illness in a hospital emergency department, during two different epidemic seasons. Consecutive patients attending an emergency department during two study periods (February/March 2009 and 2010) were enrolled using broad inclusion criteria (fever/preced...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22205
更新日期:2011-11-01 00:00:00
abstract::The present study compared the efficacy of standard and extended durations of PEG-IFNα-2a monotherapy for treatment of HBeAg-negative Chinese patients with chronic hepatitis B. Patients were randomized to receive standard therapy (n = 38; 48 weeks treatment) or extended therapy (n = 42; 72 weeks treatment). Extended t...
journal_title:Journal of medical virology
pub_type: 杂志文章,随机对照试验
doi:10.1002/jmv.24013
更新日期:2014-10-01 00:00:00
abstract::Comparison of RT-PCR assays established in house at various places revealed that laboratories could differ in sensitivity by as much as 1,000-fold in terms of the ability to detect measles virus sequences in clinical samples. The study indicates that PCR findings, positive or negative, are questionable if they are not...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10371
更新日期:2003-05-01 00:00:00
abstract::Little information about immunity to respiratory syncytial virus (RSV) and disease pathogenesis in elderly persons exists. Humoral immunity to RSV was assessed in 41 young, 56 healthy elderly, and 49 frail elderly adults by measuring baseline RSV specific IgG by enzyme immunoassay (EIA) and microneutralization assay (...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199910)59:2<221::aid-jmv16
更新日期:1999-10-01 00:00:00
abstract::A simple method is described for the precise quantification of infectious duck hepatitis B virus (DHBV) in cell culture, using a radioimmunofocus assay (RIFA). Primary duck hepatocyte cell cultures were infected with serial dilutions of viral samples as for a plaque assay, but then maintained with liquid overlay mediu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00