Nucleotide sequence of hepatitis B virus isolated from subjects without serum anti-hepatitis B core antibody.

Abstract:

:The nucleotide sequences of the precore/core and X open reading frames (ORFs) of hepatitis B virus (HBV) were studied in four subjects who were serologically negative for anti-hepatitis B core antibody. These subjects were positive for serum hepatitis B surface antigen and were considered to be asymptomatic HBV carriers. Sequencing of the precore/core ORF revealed precore wild type and 3 to 8 nucleotide substitutions (replacing 0 to 2 amino acids) in the core region compared with the sequence of subtype adr. These substitutions were not considered to have changed the epitope of the core antigen, resulting in the absence of anti-HBc as determined by a conventional diagnostic kit. The X ORF showed 1 to 5 nucleotide substitutions (replacing 1 to 3 amino acids) and the structure of the X protein and the core promoter/enhancer II complex appeared to be conserved. These findings strongly suggest that the absence of serum anti-HBc is not due to mutation of the HBV DNA but to an aberrant immune reaction of the host to HBV.

journal_name

J Med Virol

authors

Gotoh K,Mima S,Uchida T,Shikata T,Yoshizawa K,Irie M,Mizui M

doi

10.1002/jmv.1890460306

subject

Has Abstract

pub_date

1995-07-01 00:00:00

pages

201-6

issue

3

eissn

0146-6615

issn

1096-9071

journal_volume

46

pub_type

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