Abstract:
:A method for the absorption of false radioimmunoassay (RIA) IgM titres against herpes simplex virus (HSV) and cytomegalovirus (CMV) is presented. The serum specimens were absorbed by a mixture of protein A-Sepharose and protein A-Sepharose saturated with normal human gamma globulin (PAS/IgG). The detection of rheumatoid factor of IgM class (IgM-RF) as well as antinuclear antibodies (ANA) of both IgM and IgG class by solid-phase RIA is also described, and their role in the false IgM results was studied. It was found that the PAS/IgG absorption removed 50-90% of both IgM-RF and total IgG. The reduction of IgM-ANA clustered at 50-90% or nothing, whereas the reduction of IgG-ANA was approximately 50%. The studies with HSV and CMV antigens indicated that the removal of false IgM titres was more effective than the removal of each of these four factors. It was concluded that the IgM-RF titres alone were not sufficiently high to explain the false IgM results, but the ANA activity probably contributed.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Torfason EG,Diderholm Hdoi
10.1002/jmv.1890100302subject
Has Abstractpub_date
1982-01-01 00:00:00pages
157-70issue
3eissn
0146-6615issn
1096-9071journal_volume
10pub_type
杂志文章abstract::To report the clinical characteristics and potential risk factors of patients with coronavirus disease 2019 (COVID-19) in Wuhan Stadium Cabin Hospital in Hubei Province. A total of 571 patients of COVID-19 treated in the Wuhan Stadium Cabin Hospital were selected for analysis, univariable and multivariable logistic re...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26414
更新日期:2021-02-01 00:00:00
abstract::A seroprevalence study was carried out of six different human pathogenic viruses, namely human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), human T-cell leukemia virus (HTLV), human herpesvirus type 8 (HHV-8), and dengue virus among pregnant women and blood donors from rural (Nouna) ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20593
更新日期:2006-05-01 00:00:00
abstract::An epidemiological and molecular study of hepatitis C virus (HCV) infection was carried out in Brazilian hemodialysis centers. A total of 1,095 patients in all 15 hemodialysis centers in the State of Goiás, Brazil, were studied. All patients were interviewed for possible risk factors to HCV infection and serum samples...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20932
更新日期:2007-09-01 00:00:00
abstract::Rapid PCR-based influenza tests are increasingly used as point-of-care diagnostics in hospitals and clinics. To our knowledge, no prior studies have described clinical outcomes with implementation of rapid PCR-based influenza tests in hospitalized adult inpatients. Electronic medical records were used to assess differ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24279
更新日期:2015-12-01 00:00:00
abstract::During 1999-2000, a sustained mumps outbreak in the highly vaccinated population in Singapore was attributed to vaccine failure associated with the Rubini vaccine strain. To explain this phenomenon, the complete nucleotide and amino acid sequences of the hemagglutinin-neuraminidase (HN) gene of eight mumps virus isola...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10393
更新日期:2003-06-01 00:00:00
abstract:BACKGROUND:Seasonal influenza is an annual occurrence that leads to large community outbreaks and increased hospitalisation. A number of studies have suggested that influenza A (FLUAV) is associated with increased rates of hospitalisation and mortality compared to influenza B (FLUBV). This study compared demographic an...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26600
更新日期:2020-10-15 00:00:00
abstract::Human herpesvirus-8 (HHV-8) causes Kaposi's sarcoma (KS) and lymphoproliferative disorders in both HIV-infected and uninfected patients. HHV-8 has a worldwide occurrence but infection rates vary according to a combination of geographic and behavioral risks. The main transmission route seems to be sexual, nevertheless,...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20029
更新日期:2004-04-01 00:00:00
abstract::A small percentage of renal patients become infected with the BK polyomavirus (BKV), a pathogenic virus that causes BKV-associated nephropathy (BKVN), after kidney transplantation. This study presents a simple, rapid, high-throughput method for BKV genotyping using high-resolution melt analysis (HRMA). Using this nove...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22239
更新日期:2011-12-01 00:00:00
abstract::Enteric adenoviruses are important etiological agents associated with sporadic infections and outbreaks of acute gastroenteritis in infants and children. Fecal samples were collected from 439 hospitalized patients in the years 2005-2007 from Pune, Aurangabad, and Nagpur cities of western India to identify the most pre...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21331
更新日期:2009-01-01 00:00:00
abstract::Rotavirus-specific subclass antibody responses and cytokines, tumour necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-8 (IL-8), and IL-10, were measured in children 7-24 months of age with rotavirus diarrhoea (n = 29); the responses were compared with children with watery diarrhoea from who...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10280
更新日期:2003-02-01 00:00:00
abstract::High risk types of human papillomaviruses E6/E7 oncogenes and their association with tumor suppressor genes products are the key factors of cervical carcinogenesis. This study proposed them as specific markers for cervical dysplasia screening. The aim of the study is to compare the clinical and prognostic significance...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24199
更新日期:2015-09-01 00:00:00
abstract::One hundred twenty strains of adenovirus types 1, 2, 5, and 6 (subgenus C), isolated at various times and places, were tested by neutralization and hemagglutination inhibition with two antisera of each type. Except for one type 5 strain they reacted largely type-specifically in both tests and neither qualitative nor q...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890180310
更新日期:1986-03-01 00:00:00
abstract::The influence of an acute Epstein-Barr virus (EBV) infection on the serological parameters of persistent varicella-zoster virus (VZV) and herpes simplex virus (HSV) was studied. Sera from 161 patients with infectious mononucleosis caused by EBV and 178 age-matched controls were tested for HSV- and VZV-specific IgA. 98...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890440308
更新日期:1994-11-01 00:00:00
abstract::Human herpesvirus 6 (HHV-6) is classified as two distinct species: HHV-6A and B. HHV-6B infection can cause several clinical manifestations in transplant recipients including encephalitis, bone marrow suppression, and pneumonitis. In contrast to HHV-6B, the clinical features of HHV-6A infection remain largely undefine...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24513
更新日期:2016-09-01 00:00:00
abstract::Detection of respiratory viruses using polymerase chain reaction (PCR) is sensitive, specific and cost effective, having huge potential for patient management. In this study, the performance of an in-house developed conventional multiplex RT-PCR (mRT-PCR), real time RT-PCR (rtRT-PCR) and Luminex xTAG(®) RVP fast assay...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24299
更新日期:2016-01-01 00:00:00
abstract::Investigation of varicella-zoster virus (VZV) is important epidemiologically, and determination of its prevalence rate in human trigeminal ganglia is important to provide surveillance data. To date, studies on VZV detection in trigeminal ganglia have used specimens obtained from a relatively limited number of cadavers...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21687
更新日期:2010-02-01 00:00:00
abstract::Our studies of rotavirus challenge in adult volunteers enabled us to evaluate the relationship of pre-existing antirotavirus fecal IgA antibody to infection and illness and to investigate the local response to this infection. No relationship could be found between the pre-existing levels of fecal antirotavirus IgA ant...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890200402
更新日期:1986-12-01 00:00:00
abstract::Type 1 immunity appears to be diminished in healthy Japanese carriers of human T-lymphotropic virus type I (HTLV-I), but type 2 status remains undetermined. To further examine the subclinical effect of HTLV-I on host immunity, we measured serum antibodies to the Epstein-Barr virus (EBV) in 415 healthy Japanese adults ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20633
更新日期:2006-06-01 00:00:00
abstract::Noroviruses (called formerly "Norwalk-like viruses") cause food-borne gastroenteritis outbreaks. These outbreaks were thought to be related to shellfish consumption, although non-shellfish related outbreaks also occurred frequently in Japan. To clarify the epidemiology of Norovirus outbreaks, 435 stool samples were co...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20455
更新日期:2005-10-01 00:00:00
abstract::Primary HIV-1 infections (PHI) with non-B subtypes are increasing in developed countries while transmission of HIV-1 harboring antiretroviral resistance-associated mutations (RAMs) remains a concern. This study assessed non-B HIV-1 subtypes and RAMs prevalence among patients with PHI in university hospitals of Marseil...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25228
更新日期:2018-10-01 00:00:00
abstract::Antigenic drift of the hemagglutinin (HA) and neuraminidase (NA) proteins of the influenza virus cause a decrease in vaccine efficacy. Since the information about the evolution of these viruses in Saudi is deficient so we investigated the genetic diversity of circulating H1N1 viruses. Nasopharyngeal aspirates/swabs co...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25759
更新日期:2020-03-11 00:00:00
abstract::A rapid technique for the detection of hepatitis C virus (HCV) RNA in liver section using nonisotopic in situ hybridization was described. Only 3 of the 10 patients seropositive for antibody to HCV and HCV RNA had detectable HCV RNA in the hepatocytes (1%, 2%, and 15% of cells positive). No specific signal was detecte...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890420313
更新日期:1994-03-01 00:00:00
abstract::Human immunodeficiency virus 2 (HIV-2) infection is characterized by a slower disease progression and lower transmission rates. The molecular features that could be assigned as directly involved in this in vivo phenotype remain essentially unknown, and the importance of HIV-2 as a model to understand pathogenicity of ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21619
更新日期:2009-11-01 00:00:00
abstract::This is an audit of a new technique to improve the colposcopy service. Samples were tested for high risk HPV DNA using Digene Hybrid Capture II. Sixty-four percent of the sampled women under 30 had detectable high risk HPV DNA, decreasing to 44% in 30--39 year olds and to 27% in women over 40. High risk HPV prevalence...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20401
更新日期:2005-08-01 00:00:00
abstract::Recently, it was demonstrated that the Varicella-zoster virus (VZV) infection led to an activation of MAP kinases. The viral protein encoded by ORF61 is a major effector of JNK/SAPK and p38/MAPK phosphorylation. ORF61 shows homology to HSV-1 ICP0, a multifunctional protein that influences the activity of c-Jun in infe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20373
更新日期:2005-07-01 00:00:00
abstract::The performance of French virology laboratories belonging to the ANRS network has been assessed annually for 3 years. The performance of these laboratories was compared between the years 2002 and 2003. Ten and 7 coded samples were sent to 38 virology laboratories in 2002 and 45 virology laboratories in 2003, respectiv...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20522
更新日期:2006-02-01 00:00:00
abstract::Recently developed monoclonal antibodies against the 42,000-dalton major inner capsid protein were used in an enzyme immunoassay to subgroup a total of 156 rotavirus specimens obtained from Japanese infants and young children with acute gastroenteritis during the period between December 1981 and April 1983. Only 2 spe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890170105
更新日期:1985-09-01 00:00:00
abstract::To investigate whether vertical transmission of the human herpesvirus 8 (HHV-8) may occur during pregnancy or at delivery, we enrolled 295 women recruited attending the Division of Obstetrics and Gynecology of a University Teaching of Rome Tor Vergata, S. Eugenio Hospital. The study population was divided in two group...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20012
更新日期:2004-03-01 00:00:00
abstract::Despite prolonged undetectable plasma viral load some HIV-1 infected patients have been reported to develop resistance-associated mutations leading to treatment failure. The mechanisms for this phenomenon and the point of origin for residual viral evolution are still not elucidated. In order to quantify cell-associate...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20993
更新日期:2007-10-01 00:00:00
abstract::Immune-specific IFN (IFN) is produced by the peripheral blood mononuclear leukocytes (PBML) of greater than 95% of HSV-seropositive humans with infrequent recurrences of herpes labialis [Green, Yeh, and Overall, 1981]. However, herpes virus-induced immune-specific IFN was produced by PBML from only 33 of 48 (68.8%) pe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890160311
更新日期:1985-07-01 00:00:00