Abstract：
:Recent epidemiological and immunohistochemical studies have indicated a possible link between measles virus and inflammatory bowel disease (IBD). The aim of this study was to use a sensitive and robust method for the detection of measles virus RNA in IBD and control clinical samples. Peripheral blood mononuclear cells and intestinal resection tissue from IBD and control patients were studied. Two methods were used to determine the presence of measles virus RNA: hybrid capture, using measles virus-specific oligonucleotides linked to paramagnetic solid-phase supports, was carried out on total cellular RNA to enrich for measles virus RNA sequences. Reverse transcription followed by the polymerase chain reaction (RT-PCR) using rTth DNA polymerase was employed for amplification of measles virus N-gene sequences amongst the enriched species. Total RNA was also used for RT-PCR of a housekeeping mRNA species to assess RNA quality. RT-PCR for another region of the measles genome (the haemagglutinin (H) gene) was also undertaken in order to confirm the results obtained using N-gene primers for analysis of these samples. None of the samples were positive for measles N- or H-gene RNA using RT-PCR. Positive control samples confirmed the sensitivity of the methods employed. These results show that either measles virus RNA was not present in the samples, or was present below the sensitivity limits known to have been achieved.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Chadwick N,Bruce IJ,Schepelmann S,Pounder RE,Wakefield AJsubject
Has Abstractpub_date
1998-08-01 00:00:00pages
305-11issue
4eissn
0146-6615issn
1096-9071pii
10.1002/(SICI)1096-9071(199808)55:4<305::AID-JMV9>journal_volume
55pub_type
杂志文章abstract：:Immunoglobulin class-specific determination by ELISA and a virus neutralization test (NT) were compared for the detection of the serum antibody responses after vaccination of infants with the RIT 4237 rotavirus vaccine (of bovine origin). A capture method was applied for specific IgM determination by ELISA because of ...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.1890210306
更新日期：1987-03-01 00:00:00
abstract：:Early estimates of the prevalence of TTV viremia in healthy adults of developed countries were in the order of 1--10 %, while similar estimates in Third World countries were considerably higher. Using three different PCRs, TTV-related DNA was detected in serum from 180 out of the 201 Norwegian blood donors tested, ind...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.1062
更新日期：2001-07-01 00:00:00
abstract：:Knowledge of the precise timing of SARS-CoV-2 infection may be of clinical and epidemiological relevance. The presence of low-avidity IgGs has conventionally been considered an indicator of recent infection. Here, we carried out qualitative assessment of SARS-CoV-2-specific antibody avidity using an urea (6M) dissocia...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.26344
更新日期：2021-02-01 00:00:00
abstract：:Enterovirus 68 (EV-D68) was associated with mild to severe respiratory infections. In the last 4 years, circulation of different EV-D68 strains has been documented worldwide. In this study, the phylogenetic characterization of nine EV-D68 strains identified in patients in the 2010-2012 period and 12 additional EV-D68 ...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.23821
更新日期：2014-09-01 00:00:00
abstract：:Development of hepatitis B virus (HBV)-resistant strains following nucleos(t)ide analog treatment is a major medical concern. This report describes a case of an adult patient with chronic HBV infection, sequentially treated with the nucleos(t)ide analogues, lamivudine, adefovir, and entecavir. During monotherapy with ...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.20982
更新日期：2007-11-01 00:00:00
abstract：:Limited data are available describing human papillomavirus (HPV) genotype distribution among females with cytological abnormalities in Switzerland. Cervical cell specimens obtained from 5,318 women were screened routinely by liquid-based Pap smear. All specimens with cellular abnormalities were analyzed subsequently f...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.22126
更新日期：2011-08-01 00:00:00
abstract：:The natural history of chronic hepatitis B virus (HBV) infection is highly variable, ranging from a benign course to one of shortened life expectancy. Liver histology represents an accurate tool for assessing progressive liver disease, and has been used in five recent Phase III clinical trials of the oral nucleoside a...
journal_title：Journal of medical virology
pub_type： 杂志文章,评审
doi：
更新日期：2000-07-01 00:00:00
abstract：:The JC virus (JCV) is a common human virus persisting in renal tissue. In immunocompromised individuals it may reactivate and cause progressive multifocal leukoencephalopathy (PML). JCV has also been implicated in cancerogenesis, leading to various brain tumors and cancers of gastrointestinal tract. In this study the ...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.21153
更新日期：2008-06-01 00:00:00
abstract：:As the coronavirus disease 2019 pandemic keep tackling global public health systems worldwide. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) genome keeps mutating. In that regard, the recent emergence of the B.1.1.7 lineage in the UK has called the attention of global authorities. One point of concer...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.26823
更新日期：2021-01-28 00:00:00
abstract：:False negativity in a commercial anti-HIV kit (IMx HIV-1/HIV-2 3rd Generation Plus (code 8B32) was investigated, and the kit that superseded it (IMx HIV-1/HIV-2 III Plus, code 8C98) was evaluated. In a comparison on 574 freshly collected anti-HIV-1-positive specimens, 97.2% were more reactive in 8C98 than in 8B32; 35....
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:We have previously reported that concanavalin A-immobilized polystyrene nanospheres (Con A-NS) could efficiently capture HIV-1 particles and that intranasal immunization with inactivated HIV-1-capturing nanospheres (HIV-NS) induced vaginal anti-HIV-1 IgA antibody response in mice. In this study, to evaluate the protec...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.20100
更新日期：2004-07-01 00:00:00
abstract：:Coronavirus disease-2019 (COVID-19) infection and its severity can be explained by the concentration of glycosylated severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) viral particles in the lung epithelium, the concentration of glycosylated angiotensin-converting enzyme receptor 2 (ACE2) in the lung epithel...
journal_title：Journal of medical virology
pub_type： 杂志文章,评审
doi：10.1002/jmv.25887
更新日期：2020-07-01 00:00:00
abstract：:This open, randomized study was conducted in healthy Chinese youngsters, aged between 10 and 19 years to compare the reactogenicity and immunogenicity of two vaccines: the combined vaccine against hepatitis A and B was administered in a two-dose schedule with the profile of the corresponding monovalent vaccines, while...
journal_title：Journal of medical virology
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1999-09-01 00:00:00
abstract：:Stool specimens from 334 infants and young children hospitalized with diarrhea in the General Hospital, Kuala Lumpur, Malaysia between August and November, 1987 were analyzed for the presence of rotavirus double-stranded (ds) RNA by polyacrylamide gel electrophoresis. Of the 334 specimens analyzed, 32 (9.6%) were posi...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.1890430302
更新日期：1994-07-01 00:00:00
abstract：:In order to demonstrate the viral specificity of IgM-containing immune complexes (IgM-CIC) detected by a C1q assay in renal allograft recipients developing a CMV infection, a technique is described allowing: 1) the dissociation of IgM-CIC by action of an acid buffer, and 2) the characterization of the viral specificit...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.1890330312
更新日期：1991-03-01 00:00:00
abstract：:Female renal transplant recipients have an increased incidence of human papillomavirus (HPV) associated lesions, such as cervical dysplasia and neoplasia [Schneider et al., 1983]. In this study we tested the serological responses by enzyme-linked immunoadsorbent assays (ELISA) to 3 different antigenic regions of HPV t...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.1890400304
更新日期：1993-07-01 00:00:00
abstract：:To design a safe and effective synthetic peptide vaccine against rubella virus (RV) infection, it is necessary to identify immunodominant T-cell epitopes of RV structural proteins. To define such epitopes, 49 overlapping synthetic peptides (17-34 residues in length) corresponding to more than 95% of the amino acid seq...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.1890400302
更新日期：1993-07-01 00:00:00
abstract：:The human papillomavirus (HPV) 16 genome has been studied extensively, although no study has focused on the E1 gene that is implicated in viral DNA replication. After analyzing the E1 region of HPV 16 genomes in 429 cervical samples, 11.2% were found to contain a 63 nucleotides duplication in this region. Sequence ana...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.21304
更新日期：2008-12-01 00:00:00
abstract：:To study the association between hepatitis B and schistosomiasis, 1,234 Egyptian males, ages 18 to 24, were interviewed, examined, and tested for Schistosoma mansoni infection and HBsAg. Sera from 91 (7.4%) of the study subjects were positive for HBsAg, and S. mansoni was found in the stools of 26.3%. There was no cor...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.1890230203
更新日期：1987-10-01 00:00:00
abstract：:The role of a novel virus, designated as TT virus (TTV), as a cause of chronic liver disease has not been well defined. We investigated the prevalence of TTV among 69 patients with chronic liver disease of unknown etiology and 50 volunteer blood donors with normal transaminase levels. TTV DNA was amplified by polymera...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/1096-9071(200011)62:3<392::aid-jmv12>3.0.c
更新日期：2000-11-01 00:00:00
abstract：:The epidemiology of HTLV-I infection in female prostitutes was studied in a survey of 395 prostitutes from Callao, Peru (the port city of Lima), 72 prostitutes from Iquitos, Peru (another port city on the Amazon River), and 510 prenatal clinic patients from Lima. Prostitutes reported a mean of 8.8 years (range, 1-39 y...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.1890380110
更新日期：1992-09-01 00:00:00
abstract：:BK polyomavirus (BKV) is a ubiquitous virus in humans that remains latent in the urogenital tract after a primary infection during childhood. The virus, which is reactivated frequently and excreted in urine, can cause nephropathy in renal transplant recipients. BKV sequences are classified into four subtypes (I-IV). S...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.22035
更新日期：2011-04-01 00:00:00
abstract：:Genotype-specific effects of parvovirus B19 (B19V) infections on left ventricular function in patients with dilated cardiomyopathy (DCM) have not been investigated so far. In this prospective clinical study, the prevalences of B19V genotypes in endomyocardial biopsies from patients presenting with inflammatory heart d...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.22187
更新日期：2011-10-01 00:00:00
abstract：:An enzyme immunoassay utilizing a fluorogenic substrate (EIFA) was employed to compare humoral antibody responses to purified virion and dense body antigens in human cytomegalovirus (CMV) infections. Results of these antibody assays were also compared to those obtained by plaque reduction neutralization and complement...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.1890060204
更新日期：1980-01-01 00:00:00
abstract：:Noroviruses are an important cause of acute gastroenteritis. Increasing data showed that the GII-4 strains are predominant worldwide and new GII-4 variants emerge every 1-2 years causing major epidemics. Surveillance of gastroenteritis in hospitalized children under 5 years of age in China is described. Among 1,110 sp...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.21308
更新日期：2008-11-01 00:00:00
abstract：:Immunological factors, such as cytokines, have been proposed as a cause of changes in the lipid profile of dengue patients. We studied whether serum lipid levels and serum TNF-α levels are associated in a group of dengue patients from an endemic region in the Northwest of Mexico. We found statistically important diffe...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.25056
更新日期：2018-06-01 00:00:00
abstract：:During the 1998-1999 influenza season, two distinct influenza B virus Yamagata group strains were isolated from the patients of a private clinic. Each responded differently to monoclonal antibodies (Mabs) 5H4 and 8B3 on staining, and hemagglutination inhibition and neutralizing tests. When the analysis of nucleotide s...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.2099
更新日期：2001-12-01 00:00:00
abstract：:This study was designed to determine the prognostic value of erythrocyte adenosine deaminase (ADA) as a possible indicator of progression to AIDS, and compare this with other known cellular and serological markers. At the end of a 3-year study, a cohort of 114 human immunodeficiency virus-1 (HIV-1) seropositive intrav...
journal_title：Journal of medical virology
pub_type： 临床试验,杂志文章
doi：10.1002/jmv.1890450216
更新日期：1995-02-01 00:00:00
abstract：:N-Acetylethyleneimine (AEI) was used to inactivate the avirulent Togavirus Semliki Forest virus (A774 strain) grown in chick embryo, Vero, and brain cell cultures. The purity of the virus preparation affected the kinetics of inactivation. The rate of inactivation increased with a rise in temperature from 5 to 40 degre...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.1890190409
更新日期：1986-08-01 00:00:00
abstract：:Sera from 31 human immunodeficiency virus (HIV)-infected patients, representing different clinical stages of HIV infection, were assayed for antibodies against measles and mumps viruses by various serological tests and compared to 23 healthy controls. Sera from four patients (two primary, one asymptomatic, and one acq...
journal_title：Journal of medical virology
pub_type： 杂志文章
doi：10.1002/jmv.1890260107
更新日期：1988-09-01 00:00:00
