Abstract:
:N-Acetylethyleneimine (AEI) was used to inactivate the avirulent Togavirus Semliki Forest virus (A774 strain) grown in chick embryo, Vero, and brain cell cultures. The purity of the virus preparation affected the kinetics of inactivation. The rate of inactivation increased with a rise in temperature from 5 to 40 degrees C and in concentration of AEI from 0.025 to 0.1%. The resultant vaccine was inoculated into adult mice to test its antigenicity and into suckling mice to test for the presence of infective virus. Semliki Forest virus-specific IgG was produced equal to that of mice given live virus, and mice were protected against the lethal SFV L10 strain. No suckling mice died, and the brains of the adult mice showed no pathology.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Amor S,Webb HEdoi
10.1002/jmv.1890190409subject
Has Abstractpub_date
1986-08-01 00:00:00pages
367-76issue
4eissn
0146-6615issn
1096-9071journal_volume
19pub_type
杂志文章abstract::To elucidate the mechanism of heterologous antibody responses to herpes simplex virus (HSV) and varicella-zoster virus (VZV) which occur in some patients with HSV or VZV infections, stronger evidence was sought for the existence of cross-reacting antibodies to these viruses, using antibody absorption procedures. Absor...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890090105
更新日期:1982-01-01 00:00:00
abstract::We designed experiments to assess the effects of binding of the HSV-2 Fc receptor (FcR) to purified rabbit nonimmune IgG and purified Fc. Purified Fc (75 micrograms) or nonimmune IgG (100 micrograms) when bound to HSV-2 did not reduce infectivity but did protect the virions against thermal inactivation at 37 degrees C...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890130307
更新日期:1984-01-01 00:00:00
abstract::Herpes simplex virus (HSV) DNA in the cerebrospinal fluid (CSF) of children with herpes simplex encephalitis (HSE) was quantified and typed using the polymerase chain reaction (PCR) assay. During the acute phase, HSV-DNA was detected in the CSF of 13 patients with HSE, including 5 neonates. A restriction profile of th...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890410214
更新日期:1993-10-01 00:00:00
abstract::Responses to influenza vaccination are poor in frail elderly subjects who suffer the greatest morbidity and mortality due to infection. Therefore, a randomized clinical trial was performed to determine the effect of a double dose and booster vaccination on antibody responses after influenza vaccination. A total of 815...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/1096-9071(200104)63:4<293::aid-jmv1004>3.0
更新日期:2001-04-01 00:00:00
abstract::The worldwide infection rate of herpesvirus is high, but the detailed prevalence in China, especially the central area, remains unclear. In the present study, the prevalence of herpes simplex virus (HSV), Epstein-Barr virus (EBV), and human cytomegalovirus (HCMV) was investigated in 303 healthy adults in Wuhan, a repr...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24989
更新日期:2018-03-01 00:00:00
abstract::Physicochemical studies of hepatitis B e antigen (HBeAg) revealed a clear cut difference between e1 and e2 antigen. The e1 antigen was found to have a MW of Ca 150,000 and a pI of 6.4-7.2, whereas both the MW and pI of the e2 antigen were heterogeneous depending upon the source of serum. Sera obtained from asymptomati...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890040105
更新日期:1979-01-01 00:00:00
abstract::Thirty-two cerebrospinal fluid (CSF) samples from eighteen patients with confirmed herpes simplex encephalitis (HSE) were assayed by an indirect enzyme-linked immunosorbent assay (ELISA) for the presence of viral antigens. The results are expressed as an antigen ratio distinguishing between herpes simplex virus (HSV) ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890210209
更新日期:1987-02-01 00:00:00
abstract::HCV strains belonging to genotype 4 may be gaining endemicity across Continental Europe but the extent of their spread in the United Kingdom is unknown. Sera referred from patients attending hospitals in England between 2004 through 2008 were characterised. A total of 243 sera carried HCV genotype 4. The most common s...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24069
更新日期:2015-03-01 00:00:00
abstract::Inoue-Melnick virus (IMV) was stabilized to thermal inactivation at 50 degrees C by 1 M Na2SO4 but not by 1 M MgCl2, 1 M MgSO4, 2 M NaCl, and 2 M KCl. The latter salts enhanced the inactivation over that in distilled water. Na2SO4 stabilized IMV at 1 M concentration but enhanced inactivation at 0.1 M. Three types of I...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890200203
更新日期:1986-10-01 00:00:00
abstract::The effect of different formulations of interferon on therapeutic response in patients coinfected with HIV and HCV is unclear. In this study, the safety, tolerability, viral kinetics (VK) modeling and host responses among HIV/HCV coinfected patients treated with pegylated-IFN or albinterferon alfa-2b (AlbIFN) with wei...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23773
更新日期:2014-02-01 00:00:00
abstract::In order to assess the efficacy of interferon-alpha on hepatitis C viral RNA in patients with chronic hepatitis C, we analyzed the levels of HCV RNA in sera and liver tissues of 16 patients pre- and posttreatment using a semiquantitative polymerase chain reaction method. Fifteen of 16 patients were positive for anti-H...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1002/jmv.1890400114
更新日期:1993-05-01 00:00:00
abstract::High risk types of human papillomaviruses E6/E7 oncogenes and their association with tumor suppressor genes products are the key factors of cervical carcinogenesis. This study proposed them as specific markers for cervical dysplasia screening. The aim of the study is to compare the clinical and prognostic significance...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24199
更新日期:2015-09-01 00:00:00
abstract::Outbreaks of gastroenteritis are a major public health problem in New Zealand. The introduction of molecular detection methods has now shown that the 'Norwalk-like viruses' (NLVs) are the major cause of food and waterborne nonbacterial gastroenteritis. Reverse transcription and polymerase chain reaction (RT-PCR) were ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1018
更新日期:2001-05-01 00:00:00
abstract::It have been suggested that hepatitis B virus (HBV) pre-S deletions may play a role in hepatocarcinogenesis. The aim of the study was to determine the prevalence of pre-S deletions in chronic hepatitis B patients in Hong Kong, the factors associated with the deletions and its relationship with hepatitis B e antigen (H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21901
更新日期:2010-11-01 00:00:00
abstract::A total of 18 rotavirus G9 strains in South Korea were collected during five rotavirus seasons between 2005 and 2010. The relationship between these strains was examined by analyzing the genetic variation of two major structural genes, VP7 and VP4. All the rotavirus isolates were of the G9P[8] genotype. The VP7 phylog...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23436
更新日期:2013-01-01 00:00:00
abstract::Over the past decade, a G4P[6] strain has been found to be circulating in different neonatal wards in the Pretoria area. This endemic strain was associated with both asymptomatic and symptomatic infection, providing the opportunity to undertake a molecular study of some of the putative "virulence" genes. The genes enc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/1096-9071(200010)62:2<208::aid-jmv12>3.0.c
更新日期:2000-10-01 00:00:00
abstract::A multiplex polymerase chain reaction (PCR) assay that is capable of detecting and typing six serotypes of respiratory adenovirus (Ad) was developed, using multiple sets of type-specific primers. The detection of each different serotype depended on distinguishing different numbers and sizes of amplification products o...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2174
更新日期:2002-04-01 00:00:00
abstract::Enteroviruses (EVs) often infect humans, presenting as endemic or epidemic infections. In this study, the diversity of infecting EVs was studied among 146 children and 137 adults in a small community in Cameroon. The participants provided 2,458 fecal samples during 1-year monthly collection; 10 or more samples were ob...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23926
更新日期:2014-12-01 00:00:00
abstract::The human respiratory syncytial virus (HRSV) is the most important cause of admission to hospital for acute lower respiratory tract infections in infants and young children worldwide. Only few studies have investigated the molecular evolution of HRSV, and none has been conduct ed in Italy. The genetic diversity of the...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21012
更新日期:2007-12-01 00:00:00
abstract::One hundred twelve peripheral blood specimens were tested for the presence of cytomegalovirus (CMV) by the tube culture indirect immunoperoxidase (TC-IPA) procedure, the shell vial assay [shell vials were pre- and postinoculation treated with medium containing 2 of 10% fetal bovine serum (FBS) or 100 micrograms% corti...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890380109
更新日期:1992-09-01 00:00:00
abstract::Persisting human papillomavirus (HPV) infection is a critical step in cervical carcinogenesis. This study was conducted to determine the type-specific HPV persistence and risk factors for persistence of high-risk HPV infections in a large cohort of Danish women. The study was based on a population-based prospective co...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21750
更新日期:2010-04-01 00:00:00
abstract::Oncogenic human papillomavirus (HPV), a causative agent of uterine cervical cancer, has also been detected in head and neck squamous cell cancers, especially in squamous cell carcinomas of the tonsils. However, the true HPV prevalence in normal and neoplasic oropharyngeal mucosa remains uncertain. To determine the pre...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20583
更新日期:2006-05-01 00:00:00
abstract::This study aimed to examine the baseline prevalence of genital human papillomavirus (HPV) infection among human immunodeficiency virus (HIV)-seropositive men who have sex with men in Taiwan and to determine the association of age and CD4+ T cell counts with HPV infection. In 2010, 305 men who have sex with men infecte...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23413
更新日期:2012-12-01 00:00:00
abstract::Noroviruses (NoVs) are a leading cause of acute gastroenteritis outbreaks and sporadic cases of diarrhea in industrialized countries. To study the prevalence and genetic diversity of NoVs in Guatemala, stool specimens were collected from hospitalized and ambulatory patients presenting with diarrhea (≥3 loose or liquid...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23578
更新日期:2013-07-01 00:00:00
abstract::The influence of an acute Epstein-Barr virus (EBV) infection on the serological parameters of persistent varicella-zoster virus (VZV) and herpes simplex virus (HSV) was studied. Sera from 161 patients with infectious mononucleosis caused by EBV and 178 age-matched controls were tested for HSV- and VZV-specific IgA. 98...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890440308
更新日期:1994-11-01 00:00:00
abstract::HIV-1 replicative activity and its relation to the clinical and immunological evolution of infection was studied in a group of 150 HIV-1 seropositive Italian i.v. drug abusers over a 1 year period. HIV-1 was isolated from 90 (60%) subjects; two groups of isolates were distinguished, according to replicative activity "...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890320411
更新日期:1990-12-01 00:00:00
abstract::In vitro, beta-herpesviruses can stimulate or inhibit HIV replication under particular circumstances. In order to investigate the effects of beta-herpesvirus infection on HIV replication and vice versa at an organ level, we determined the quantitative relationships between cytomegalovirus (CMV), human herpesviruses (H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199903)57:3<278::aid-jmv11
更新日期:1999-03-01 00:00:00
abstract::The cellular source(s) and the clinical significance of persistent low-level viremia, below 50 HIV RNA copies per ml of plasma, achieved in many patients with high adherence to highly active antiretroviral therapy (HAART) remain unclear. Also, it is not clear if residual plasma HIVs during HAART can become predominant...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21366
更新日期:2009-01-01 00:00:00
abstract::The polymerase chain reaction (RNA-PCR) was used for specific detection of respiratory syncytial virus (RSV) genomes in clinical specimens. A set of primers was selected from conserved regions of the 1B and N genes for detection of both subgroups. The primers were found to be RSV specific, all RSV strains generated a ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890440115
更新日期:1994-09-01 00:00:00
abstract::Nasopharyngeal carcinoma is closely associated with Epstein-Barr virus (EBV) and the EBV encoded latent membrane protein-1 expression (LMP1) is commonly found in the tumour cells. LMP1 has been shown to be involved in modulation of cell growth in B cells but the biological properties of LMP1 expression in nasopharynge...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2112
更新日期:2002-01-01 00:00:00