Abstract:
:The objective of the study was to assess the genotypic and phenotypic properties of 18 viral strains from human immunodeficiency virus-1 (HIV-1) positive patients and to identify subtype C isolates for vaccine design strategies. All the isolates were non-syncytium-inducing (NSI) in both the primary and MT-2 cell cultures. The amino acid charge of the V3 loop correlated with the NSI phenotype of the strains. The V3 competitive peptide enzyme immunoassay and DNA sequencing of the partial gp120 region gave concordant results on the 15 subtype C strains, whereas the three B genotypes gave a positive to B, a nonreactive to B, and a dual reaction to the B-D peptides, respectively. Sixteen of the isolates used only CCR5 as coreceptor whereas two isolates made use of additional coreceptors including CXCR4. In summary, all our subtype C isolates are NSI phenotypically and almost all of them use CCR5 exclusively as their coreceptor.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Treurnicht FK,Smith TL,Engelbrecht S,Claassen M,Robson BA,Zeier M,van Rensburg EJdoi
10.1002/jmv.10199keywords:
subject
Has Abstractpub_date
2002-10-01 00:00:00pages
141-6issue
2eissn
0146-6615issn
1096-9071journal_volume
68pub_type
杂志文章abstract::The epidemiology of HTLV-I infection in female prostitutes was studied in a survey of 395 prostitutes from Callao, Peru (the port city of Lima), 72 prostitutes from Iquitos, Peru (another port city on the Amazon River), and 510 prenatal clinic patients from Lima. Prostitutes reported a mean of 8.8 years (range, 1-39 y...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890380110
更新日期:1992-09-01 00:00:00
abstract::Little information about immunity to respiratory syncytial virus (RSV) and disease pathogenesis in elderly persons exists. Humoral immunity to RSV was assessed in 41 young, 56 healthy elderly, and 49 frail elderly adults by measuring baseline RSV specific IgG by enzyme immunoassay (EIA) and microneutralization assay (...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199910)59:2<221::aid-jmv16
更新日期:1999-10-01 00:00:00
abstract::Chitin in the form of microparticles (chitin microparticles, CMP) has been demonstrated to be a potent stimulator of macrophages, promoting T-helper-1 (Th1) activation and cytokine response. In order to examine the mucosal adjuvant effect of CMP co-administered with influenza hemagglutinin (HA) vaccine against influen...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20247
更新日期:2005-01-01 00:00:00
abstract::Throughout most of the last decade, B/Yamagata/16/88-lineage influenza viruses were predominant among the B isolates circulating worldwide, whereas B/Victoria/2/87-lineage viruses were isolated infrequently and restricted geographically to eastern Asia. During the 2001-02 influenza season, B/Victoria/2/87-lineage viru...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20225
更新日期:2004-12-01 00:00:00
abstract::Hepatitis E virus (HEV), a positive-strand RNA agent, has been associated with enterically transmitted non-A, non-B hepatitis in Asia, Africa, and Mexico. To evaluate the role of HEV in an outbreak of hepatitis in Pakistan, we used immune electron microscopy to detect 1) antibody to HEV, for evidence of infection, and...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890360205
更新日期:1992-02-01 00:00:00
abstract::Human cytomegalovirus (HCMV) is the leading cause of congenital infection and an opportunistic pathogen capable of establishing lifelong latency. In the present study, we aimed to investigate the distribution of glycoprotein B, H, and N in infants of Shanghai and correlate the genotype with active and latent HCMV infe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26210
更新日期:2020-06-21 00:00:00
abstract::Long-term treatment with retrotranscriptase (RT) inhibitors eventually leads to the development of drug resistance. Drug-related mutations occur naturally and these can be found in hepatitis B virus (HBV) carriers who have never received antiviral therapy. HBsAg are overlapped with RT domain, thus nucleot(s)ide analog...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24098
更新日期:2015-06-01 00:00:00
abstract::A previous study revealed a high prevalence of hepatitis B surface antigen (HBsAg) and hepatitis delta virus (HDV) RNA among 249 apparently healthy individuals (mean+/-standard deviation age, 48.4+/-13.9 years; 126 males and 123 females) in Ulaanbaatar, Mongolia. To investigate further the prevalence of HDV infection ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20363
更新日期:2005-07-01 00:00:00
abstract::A nationwide hepatitis B vaccination program was launched in Taiwan in 1984. To study the impact of this ongoing program on hepatitis B virus (HBV) infection, a follow-up seroepidemiologic study was carried out in 1989 in a Taipei district where pre-vaccination seroepidemiology had been studied. HBV markers were studi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890340205
更新日期:1991-06-01 00:00:00
abstract::We report a male caucasian German pediatric patient of no Arab or Mediterranean ancestry with virus associated CNS lesions in Griscelli's syndrome (GS; McKusick No. 214450). The boy presented with recurrent infections, and meningitis with subsequent progressive signs of increased intracranial pressure leading to death...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199711)53:3<306::aid-jmv21
更新日期:1997-11-01 00:00:00
abstract::Crimean-Congo hemorrhagic fever (CCHF) is an infectious disease that is caused by CCHF virus. A family of transmembrane receptors called as Toll-like receptors (TLRs) selectively acts in recognizing a wide range of microbial components and endogenous molecules released by damaged tissue and have been preserved through...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24519
更新日期:2016-10-01 00:00:00
abstract::Fluorescent in situ hybridization (FISH) was used to investigate the chromosomal integration sites of human herpesvirus 6 (HHV-6) in phytohemagglutinin-stimulated leukocytes and B lymphocytes from Epstein-Barr virus transformed lymphoblastoid cell lines (LCLs). Five different chromosomal integration sites were found i...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21299
更新日期:2008-11-01 00:00:00
abstract::An enzyme immunoassay utilizing a fluorogenic substrate (EIFA) was employed to compare humoral antibody responses to purified virion and dense body antigens in human cytomegalovirus (CMV) infections. Results of these antibody assays were also compared to those obtained by plaque reduction neutralization and complement...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890060204
更新日期:1980-01-01 00:00:00
abstract:AIMS:Little data have been published so far on the epidemiological aspects of hepatitis D virus (HDV) infection in immigrant populations and even poorer is the information on the virological, phylogenetic, and clinical aspects of this infection in these populations. This review article, aimed primarily at physicians ca...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.25570
更新日期:2019-12-01 00:00:00
abstract::Subgroup A respiratory syncytial viruses present in respiratory secretions and low passage level cell culture isolates were found to be markedly less susceptible to neutralization with monoclonal antibodies (MAbs) to the F glycoprotein than the cell culture adapted A2 virus strain. Low passage virus isolates collected...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20892
更新日期:2007-06-01 00:00:00
abstract::It have been suggested that hepatitis B virus (HBV) pre-S deletions may play a role in hepatocarcinogenesis. The aim of the study was to determine the prevalence of pre-S deletions in chronic hepatitis B patients in Hong Kong, the factors associated with the deletions and its relationship with hepatitis B e antigen (H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21901
更新日期:2010-11-01 00:00:00
abstract::Mixed cryoglobulinemia is a lymphoproliferative disorder associated with hepatitis C virus (HCV). In patients chronically affected by HCV the prevalence of mixed cryoglobulinemia is variable ranging from 0% to 56%. To verify whether polyomaviruses (PyV) play a role in this disorder a total of 222 blood samples from 63...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23867
更新日期:2014-04-01 00:00:00
abstract::A highly sensitive one-step real-time RT-PCR method using a minor-groove-binding (MGB) probe was developed for detection and quantitation of severe febrile with thrombocytopenia syndrome virus (SFTSV). The assay could discriminate SFTSV infection from other related viral diseases in human with a minimum detection limi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23415
更新日期:2013-02-01 00:00:00
abstract::A common receptor for coxsackie B virus and adenovirus has been described recently in cells of human and murine origin. Since the established cell line A-549 is suitable for adenoviruses, the potential use of A-549 cells for the isolation of coxsackie B viruses from clinical samples was investigated. All throat swabs ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::The presence of antibody to hepatitis A virus (anti-HAV) in 60% of procured owl monkeys (Aotus trivirgatus) held within the United States prompted a study of recently captured A trivirgatus in Panama. Only 2 of 145 newly captured monkeys, but all of 35 A trivirgatus held within a colony for over 100 days, were found t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890100105
更新日期:1982-01-01 00:00:00
abstract::HIV excretion patterns and specific antibody responses were evaluated in blood, semen, female genital secretions, saliva, and crevicular fluid. Samples were examined for infectious virus, viral antigens, viral nucleic acid, HIV specific IgG, IgA, anti-nef, and anti-p24. Viral load in peripheral blood appeared to incre...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310409
更新日期:1990-08-01 00:00:00
abstract::Replication of HIV-1 inside host cells is dependent on both viral and host factors. MicroRNAs are small noncoding RNAs that regulate protein synthesis. MicroRNAs may control viral replication either by directly targeting the viral genome or indirectly through cellular proteins that are required during the viral lifecy...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26755
更新日期:2020-12-24 00:00:00
abstract::Recent epidemiological and immunohistochemical studies have indicated a possible link between measles virus and inflammatory bowel disease (IBD). The aim of this study was to use a sensitive and robust method for the detection of measles virus RNA in IBD and control clinical samples. Peripheral blood mononuclear cells...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract:BACKGROUND AND AIM:The hepatitis B virus (HBV) prevalence study performed in 2003 in Belgium is believed to be underestimating HBV prevalence due to underrepresentation of the non-Belgian population. Therefore, we assessed the prevalence and risk factors of HBV infection in a multi-ethnic region situated in Middle-Limb...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25457
更新日期:2019-08-01 00:00:00
abstract::Three hepatitis B surface antigen (HBsAg) preparations were compared: purified intact 22-nm HBsAg particles; HBsAg-derived, sodium dodecyl sulfate (SDS)-denatured P25 + GP30 polypeptide pool; and nondenatured P25 + GP30 micelles. The micelles had the same polypeptide composition as the P25 + GP30 pool. The immunogenic...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890110205
更新日期:1983-01-01 00:00:00
abstract::A seroprevalence study was carried out of six different human pathogenic viruses, namely human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), human T-cell leukemia virus (HTLV), human herpesvirus type 8 (HHV-8), and dengue virus among pregnant women and blood donors from rural (Nouna) ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20593
更新日期:2006-05-01 00:00:00
abstract::The prevalence of coinfection and superinfection with the delta agent was studied in 2,645 hepatitis B surface antigen (HBsAg)-positive subjects from six countries and nine islands in the Western Pacific region. The study group comprised 262 patients with acute hepatitis B and 2,383 chronic carriers of HBsAg, of whom ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890180406
更新日期:1986-04-01 00:00:00
abstract::DNA samples from recurrent condylomata acuminata biopsies of Greek males and females were examined for the presence of human papillomavirus (HPV) DNA using high-stringency Southern blot hybridization analysis. Of the twenty-six biopsies, 25 were positive for the HPV 6/11-related DNA sequences, and when further analyze...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890420311
更新日期:1994-03-01 00:00:00
abstract::A viral surveillance system in Nashville detected an outbreak of influenza B that occurred between January and March 1986. Paired sera from 32 individuals with culture-documented influenza B illness were tested using three serologic assays. Enzyme-linked immunosorbent assay (ELISA) using purified hemagglutinin-neurami...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章
doi:10.1002/jmv.1890250313
更新日期:1988-07-01 00:00:00
abstract::The present study compared the efficacy of standard and extended durations of PEG-IFNα-2a monotherapy for treatment of HBeAg-negative Chinese patients with chronic hepatitis B. Patients were randomized to receive standard therapy (n = 38; 48 weeks treatment) or extended therapy (n = 42; 72 weeks treatment). Extended t...
journal_title:Journal of medical virology
pub_type: 杂志文章,随机对照试验
doi:10.1002/jmv.24013
更新日期:2014-10-01 00:00:00