Abstract:
:An efficient method for cloning subgenomic fragments of the hepatitis B virus (HBV) was developed, utilizing its abundant single-stranded replicative intermediates. The total genomic DNA obtained from the liver tissue of patients with chronic HBV infection was treated by using the Klenow fragment of E. coli DNA polymerase I without adding any exogenous primers. Single-stranded replicative intermediates were efficiently converted to double-stranded linear DNA, one end of which terminated at (or near to) the direct repeat 1 (DR1) sequence of the HBV genome. By screening less than 1,000 recombinants from a DNA library after this treatment, we obtained a subgenomic HBV fragment of 2.0 kilobases. We then analysed HBV RNA in human liver tissue by S1 mapping. It was possible to map HBV RNA only when a DNA probe from the same tissue was used.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Kajino K,Ohta Y,Miyamura T,Saito Idoi
10.1002/jmv.1890330107subject
Has Abstractpub_date
1991-01-01 00:00:00pages
33-8issue
1eissn
0146-6615issn
1096-9071journal_volume
33pub_type
杂志文章abstract::Variation at the IL-28B locus was recently reported to be a significant predictive factor of viral response to pegylated-interferon plus ribavirin combination therapy against chronic hepatitis C. Predictive factors for the effect of therapy, including IL-28B polymorphism rs8099917 and viral and clinical factors were i...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22028
更新日期:2011-06-01 00:00:00
abstract::Crimean-Congo hemorrhagic fever infection (CCHF) is a viral zoonosis. The pathogenesis of this disease has not been established so far, however, cytokines account for its progression and outcome. The aim of the present study is to investigate the association between chemokine receptor 5 (CCR5) gene Δ32 mutation and pa...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24865
更新日期:2017-10-01 00:00:00
abstract::Rapid diagnosis of human herpesvirus primary infections or reactivations is facilitated by quantitative PCRs. Quantitative PCR assays with a standard thermal cycling profile permitting simultaneous detection of herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21095
更新日期:2008-03-01 00:00:00
abstract::It was reported previously that HCV can be transmitted from persistently infected human bone-marrow-derived B-lymphoblastoid cells (TO.FE(HCV)) to human hepatoma cells by cell-to-cell contact. The present study confirms and characterize further such type of HCV infection in vitro. TO.FE(HCV) cells were co-cultured wit...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20947
更新日期:2007-10-01 00:00:00
abstract::Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative disease (PTLD) arises in up to 10% of organ transplant recipients and is fatal in ∼50% of cases. PTLD can be modeled in SCID mice using EBV+ve human B lymphoblastoid cell lines (BLCLs), and the current study investigated intraperitoneal (ip) inocu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22164
更新日期:2011-09-01 00:00:00
abstract::Knowledge of the current epidemiology of chronic liver disease in Italy is mostly obsolete and fragmentary for the lack of up-to-date consistent data. In 2001, a 6-month prevalence study was undertaken in 79 hospitals to assess the characteristics of chronic liver disease in Italy. Both prevalent and incident cases we...
journal_title:Journal of medical virology
pub_type: 杂志文章,多中心研究
doi:10.1002/jmv.20313
更新日期:2005-04-01 00:00:00
abstract:BACKGROUND:Recent influenza B/Victoria lineage viruses contain amino acid deletions at positions 162 to 164 of the haemagglutinin (HA) protein. These amino acid deletions have affected the detection of B/Victoria lineage viruses by the lineage-specific conventional reverse-transcription polymerase chain reaction (RT-PC...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25607
更新日期:2020-03-01 00:00:00
abstract::Herpes simplex virus (HSV) infection of the corneal stroma is the most prominent cause of scar formation impairing visual acuity and HSV keratitis is the leading cause of corneal opacity throughout the world. Suriname lacked test systems for microbial causes of ocular disease, therefore a polymerase chain reaction-bas...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23414
更新日期:2012-12-01 00:00:00
abstract::Five hundred and forty-seven pregnant women with less than 32 weeks of amenorrhoea, attending an antenatal clinic of St. Camille Medical Centre (SCMC) of Ouagadougou were enrolled for a hepatitis C virus (HCV) and HIV co-infection study. Fifty-eight (10.6%) were HIV positive and 18 (3.3%) were anti-HCV positive. Only ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20258
更新日期:2005-02-01 00:00:00
abstract::Infection with oncogenic human papillomavirus (HPV) genotypes is necessary for the development of cervical cancer. Testing for HPV DNA from liquid based cervical samples can be used as an adjunct to traditional cytological screening. In addition there are ongoing viral load, genotyping, and prevalence studies. Therefo...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23882
更新日期:2014-04-01 00:00:00
abstract::The use of new Direct Acting Antivirals, specific of HCV, has greatly improved the HCV treatment. Most of the DAA are specific of HCV genotypes. Genotyping methods may target different regions of the HCV genome, though only the whole genome sequencing could confirm the correct genotype. The present study describes the...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25035
更新日期:2018-05-01 00:00:00
abstract::Previous studies provided a direct experimental evidence for the contribution of HCV core protein in the development of insulin resistance (IR), but the clinical impact of HCV core region on IR is still not clear. The present study evaluated the impact of Amino acid (aa) substitutions of HCV-1b core region on IR in 12...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21473
更新日期:2009-06-01 00:00:00
abstract::A study was carried out to determine whether hepatitis A virus (HAV) can be detected in the stools of patients hospitalized for HAV infection. Acute phase samples of whole blood and stool, as well as completed questionnaires, were obtained from 31 patients hospitalized at any of 13 hospitals in the Phoenix metropolita...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890090207
更新日期:1982-01-01 00:00:00
abstract::Between 1999 and 2007 1,388 stool specimens from patients with acute flaccid paralysis or aseptic meningitis were submitted to the Austrian reference laboratory for poliomyelitis. Samples (201) yielded non-poliovirus enterovirus in culture. One hundred eighty-one viruses were available for typing and 78 isolates which...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21374
更新日期:2009-02-01 00:00:00
abstract::A sensitive and reproducible real-time PCR assay based on TaqMan technology was developed for the detection and quantitation of hepatitis B virus (HBV) DNA in serum, and compared with an "in-house" qualitative PCR assay. HBV DNA was measured in 125 serum samples from 76 hepatitis B patients, consisting of 22 patients ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2027
更新日期:2001-10-01 00:00:00
abstract::A patient presented with acute retinal necrosis of the left eye. Demonstration of herpes simplex virus (HSV) DNA in the aqueous humour confirmed the diagnosis. Negative results of HSV type-specific antibody tests based on gG antigens suggested a primary HSV infection. However, the patient had a past history of laborat...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20083
更新日期:2004-06-01 00:00:00
abstract::HCV replication in extra-hepatic reservoirs has been suggested to occur in many tissues including PBMCs. A recent study showed evidence for compartmentalization and evolution of HCV in PBMCs. However, the cells that support HCV replication in PBMCs have not been identified. In this study we have fractionated the PBMC ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21906
更新日期:2010-12-01 00:00:00
abstract::Human papillomavirus (HPV) is known to be the cause of almost all cervical cancers. The genotypes have been classified into high and low risk types according to their oncogenic potential. However, data for many of the genotypes are limited and some (HPV-26, 53, and 66) have no agreed status. A study was undertaken to ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20330
更新日期:2005-05-01 00:00:00
abstract::Although hepatitis E virus (HEV) infection is a zoonosis, there is, currently, no standardized assay for quantitatively measuring anti-HEV antibody levels in HEV animal reservoirs. In this study, anti-HEV antibody positive serum from a rabbit (RS26) was calibrated by dose-response parallel line assay using the World H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24651
更新日期:2017-03-01 00:00:00
abstract::The incidence and the outcome of cytomegalovirus (CMV) infections were evaluated in 83 adult recipients of allogenic bone marrow transplantation. Virological and serological surveillance was performed weekly for 3 months posttransplant, and then every other week or every month until 1 year. CMV infection occurred in 4...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890230412
更新日期:1987-12-01 00:00:00
abstract::The current study aimed to investigate the diagnostic value of glycated albumin (GA), glycated hemoglobin (HbA1c), and a number of routine biomarkers as noninvasive indicators of liver fibrosis in patients with chronic hepatitis C (CHC). One hundred patients with CHC were subjected to full medical history and examinat...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26204
更新日期:2020-06-19 00:00:00
abstract::Neurologic sequelae can be devastating complications of respiratory viral infections. We report the presence of virus in neural and capillary endothelial cells in frontal lobe tissue obtained at postmortem examination from a patient infected with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Our observ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25915
更新日期:2020-07-01 00:00:00
abstract::Overall, head and neck sqamous cell carcinoma accounts for more than 550,000 cases annually worldwide. It is well known that human papillomavirus (HPV) is the main risk factor for cervical cancer development. As the incidence and the mortality of cervical cancer are closely related to the HPV prevalence, we hypothesiz...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23750
更新日期:2014-03-01 00:00:00
abstract::Human bocavirus (HBoV) was first recognized in respiratory samples in 2005. The clinical importance of HBoV infection remains unclear. This report describes the clinical features and molecular phylogeny of HBoV isolates in children with acute respiratory infections. Nasopharyngeal aspirates were obtained from 1,528 ch...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23880
更新日期:2014-12-01 00:00:00
abstract::Infection with the human papillomavirus (HPV) is responsible for 99.7% of cervical cancers, the second most prevalent neoplasia in women worldwide and the fifth leading cause of death by cancer in this population. In Chile, the incidence rate is 14.4 cases per 100,000 women per year and it is considered a significant ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22057
更新日期:2011-05-01 00:00:00
abstract::Immune correlates of protection against human cytomegalovirus (HCMV) infection are still debated. This study aimed to investigate which arm of the immune response plays a major role in protection against HCMV infection in kidney transplant recipients (n = 40) and heart transplant recipients (n = 12). Overall, patients...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25225
更新日期:2018-10-01 00:00:00
abstract::Cells from a leiomyosarcoma tumor (LMS-1) from a patient with the acquired immunodeficiency syndrome (AIDS) were explanted, cultured in vitro, and studied by phase-contrast microscopy for morphologic and growth characteristics, immunostaining for cell markers, EBER in situ hybridization and polymerase chain reaction f...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::The presence of therapy-associated hepatitis B virus (HBV) variants is the main drawback of antiviral therapy for HBV infection. Moreover, drug-resistant variants are more insensitive to a second agent and more therapy-associated mutations will be present. To apply better nucleos(t)ide analogues (NA) and reduce the oc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24006
更新日期:2015-09-01 00:00:00
abstract::Lack of recognition of human immunodeficiency virus (HIV) infection especially in children and delayed implementation of effective control programs makes HIV infection as a major cause for concern. Information on HIV epidemiology in Iran as well as other Islamic countries is limited. The aim of our study was to descri...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24294
更新日期:2016-01-01 00:00:00
abstract::The ability of hepatitis A virus (HAV) to agglutinate human erythrocytes was used to develop IgM and IgG antibody capture haemadherence tests (MACHAT and GACHAT). Haemadherence was dependent on the pH of the red cell suspension and was best in the pH range 5.4 to 5.8. The tests were applied to serum, urine, and saliva...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890390106
更新日期:1993-01-01 00:00:00