Abstract:
:Sera of patients from China with hepatocellular carcinoma (HCC) were tested for the presence of HBc/HBe- and HBx antibodies by immunoblotting using recombinant MS2 or beta gal fusion proteins as substrate. Antibodies against HBx were detected in four out of 68 HBsAg positive and in one out of three HBsAg negative sera, antibodies against HBc/HBe in 52 and two serum samples, respectively. Competition experiments in which sera were preincubated with individual viral proteins synthesized in E. coli were carried out to demonstrate the specificity of signals obtained in immunoblot analyses. In the five anti-HBx positive sera, the antibody titer against X fusion protein was higher than against core fusion protein and in one of these sera anti-x activity could be demonstrated even at a serum dilution of 1:50,000. These data indicate that X antibodies occur rarely in Chinese patients and are not serodiagnostic for HCC. The high titer of X antibodies in some patients shows that the X protein can be highly immunogenic in vivo. Induction of antibody formation may be triggered by X protein expressed from integrated viral DNA.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Liang XH,Stemler M,Will H,Braun R,Tang ZY,Schröder CHdoi
10.1002/jmv.1890250310subject
Has Abstractpub_date
1988-07-01 00:00:00pages
329-37issue
3eissn
0146-6615issn
1096-9071journal_volume
25pub_type
杂志文章abstract::Human immunodeficiency virus (HIV) is the primary etiological agent of acquired immunodeficiency syndrome (AIDS). This virus can be transmitted by blood or blood products. Homosexual males (HM) are regarded as one of the high-risk groups for AIDS. Lymphocytes obtained from HM were used for cocultivation with fresh HIV...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890230404
更新日期:1987-12-01 00:00:00
abstract::Epstein-Barr virus (EBV) serology continues to be the first diagnostic test when infectious mononucleosis is suspected. Due to possible mild immunosuppression in competitive athletes, EBV reactivation determined by increases in salivary viral load have been identified as one possible cause in recurrent respiratory inf...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23253
更新日期:2012-09-01 00:00:00
abstract::The kinetics of hepatitis B virus (HBV) and its surface antigen (HBsAg) during acute hepatitis has not yet been studied accurately in a representative number of patients. The influence of coinfecting hepatitis viruses during the acute phase of infection is not known. Three to four serum samples from 21 patients with a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10291
更新日期:2003-03-01 00:00:00
abstract::Hepatitis E virus (HEV), the sole member of the genus Hepevirus in the family of Hepeviridae, is the major cause of several outbreaks of waterborne hepatitis in tropical and subtropical countries and of sporadic cases of viral hepatitis in endemic and industrialized countries. Transmission of HEV occurs predominantly ...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.21116
更新日期:2008-04-01 00:00:00
abstract::An attempt was made to identify factors influencing the cumulative probability of an increased alanine aminotransferase (ALT) level and hepatocarcinogenesis in hepatitis C patients with a normal ALT level initially. A total of 398 consecutive patients with a normal ALT level initially for 6 months or more and follow-u...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21342
更新日期:2009-03-01 00:00:00
abstract::The presence of therapy-associated hepatitis B virus (HBV) variants is the main drawback of antiviral therapy for HBV infection. Moreover, drug-resistant variants are more insensitive to a second agent and more therapy-associated mutations will be present. To apply better nucleos(t)ide analogues (NA) and reduce the oc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24006
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVE:The unprecedented demand for testing for the ongoing coronavirus disease-2019 (COVID-19) pandemic caused by SARS-CoV-2 has led to an acute shortage and limited availability of test reagents for which pooling of samples has been recommended in areas with low prevalence. Considering the possibility of dilution ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26618
更新日期:2020-10-21 00:00:00
abstract::Hepatitis E virus (HEV) infection is a significant public health issue in many developing countries, causing waterborne outbreaks as well as sporadic hepatitis. We report here an outbreak of HEV genotype 1f infection during April-May 2018 among people living at Halisohor, a low land in the southern part of Chottogram ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26739
更新日期:2020-12-17 00:00:00
abstract::Recent evidence suggested that neurological manifestations occur in patients with a severe form of coronavirus disease (COVID-19). On the basis of this issue, neurologists are very concerned about patients with neurological disorders, especially multiple sclerosis (MS), as consumers of immunosuppressive or immune-modu...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26593
更新日期:2020-10-12 00:00:00
abstract::Varicella-zoster virus (VZV)-specific cell-mediated immunity (CMI) is critical for preventing and controlling the onset of herpes zoster (HZ). To assess VZV CMI, an interferon-γ (IFN-γ) enzyme-linked immunosorbent assay (ELISA) was validated by examining the influence of VZV-specific antigen content, incubation time, ...
journal_title:Journal of medical virology
pub_type: 杂志文章,随机对照试验
doi:10.1002/jmv.25391
更新日期:2019-05-01 00:00:00
abstract::Immunoglobulin class-specific determination by ELISA and a virus neutralization test (NT) were compared for the detection of the serum antibody responses after vaccination of infants with the RIT 4237 rotavirus vaccine (of bovine origin). A capture method was applied for specific IgM determination by ELISA because of ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890210306
更新日期:1987-03-01 00:00:00
abstract::The use of new Direct Acting Antivirals, specific of HCV, has greatly improved the HCV treatment. Most of the DAA are specific of HCV genotypes. Genotyping methods may target different regions of the HCV genome, though only the whole genome sequencing could confirm the correct genotype. The present study describes the...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25035
更新日期:2018-05-01 00:00:00
abstract::Genotype 1F was likely localized geographically to China as it has not been reported elsewhere. In this study, whole genome sequences of two rubella 1F virus isolates were completed. Both viruses contained 9,761 nt with a single nucleotide deletion in the intergenic region, compared to the NCBI rubella reference seque...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23936
更新日期:2014-12-01 00:00:00
abstract::Mutations of the precore region of hepatitis B virus (HBV) genome have been associated with fulminant and severe chronic hepatitis. However uncertainty remains about the clinical significance and transmissibility of these mutant strains. A point mutation assay (PMA) was developed to identify qualitatively and quantita...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890440105
更新日期:1994-09-01 00:00:00
abstract::Two hundred and thirty fecal specimens were collected from children (up to 5 years of age) admitted with suspected rotaviral gastroenteritis at four Irish hospitals (Cork University Hospital, Mercy Hospital, Cork, Waterford Regional Hospital, and Kerry General Hospital) in the southern region of Ireland, between 2001 ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20494
更新日期:2005-12-01 00:00:00
abstract::The effects of 6-diazo-5-oxo-L-norleucine (DON), 2-deoxy-D-glucose (DOG), and tunicamycin (TM) on the replication of poliovirus (PV) and vesicular stomatitis virus (VSV) were examined. During a 48-hr replication period, TM, DON, and DOG inhibit VSV plaque formation in HEp-2 cells by 99.9%, 99.8%, and 99.9% respectivel...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890140210
更新日期:1984-01-01 00:00:00
abstract::Hepatitis B virus (HBV) DNA may persist in the liver in the absence of serum HBV-DNA after a self-limited acute hepatitis B. This may also occur in patients with chronic hepatitis C virus (HCV) infection but its prevalence and its impact on liver histology is unknown. HBV-DNA was tested by polymerase chain reaction (P...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20073
更新日期:2004-06-01 00:00:00
abstract::Persistent Epstein-Barr virus (EBV) infection is controlled tightly by virus-specific T cells. EBV infection is reactivated intermittently over time, even in apparently healthy carriers. Changes in frequency and reactivity of memory T cells, particularly of CD8(+) origin, have not been assessed in this context. It is ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22258
更新日期:2012-01-01 00:00:00
abstract::Development of hepatitis B virus (HBV)-resistant strains following nucleos(t)ide analog treatment is a major medical concern. This report describes a case of an adult patient with chronic HBV infection, sequentially treated with the nucleos(t)ide analogues, lamivudine, adefovir, and entecavir. During monotherapy with ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20982
更新日期:2007-11-01 00:00:00
abstract::A new assay was developed for the detection of hepatitis B virus (HBV) in human serum using amplification of a short viral DNA sequence by means of the polymerase chain reaction. As little as 0.4 fg viral DNA, corresponding to about 130 genome equivalents, per ml serum could be detected after the amplification procedu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890270409
更新日期:1989-04-01 00:00:00
abstract::The aim of this study was to compare the data obtained using the new LIAISON® XL chemiluminescence system to search for HBsAg, anti-HCV, and anti-HIV1-2/p24 Ag with those obtained using the VITROS system currently adopted by the Microbiology Unit of the Hospital of Legnano. Routine samples of patients who were referre...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24648
更新日期:2017-03-01 00:00:00
abstract::The evaluation of new therapeutic resources against coronavirus disease 2019 (COVID-19) represents a priority in clinical research considering the minimal options currently available. To evaluate the adjuvant use of systemic oxygen-ozone administration in the early control of disease progression in patients with COVID...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26636
更新日期:2020-10-28 00:00:00
abstract::The effects of tumor necrosis factor-alpha and/or interferon-gamma on the replication of hepatitis B virus were examined using HB611 cells. These cells were derived from human hepatoblastoma cells, Huh6, by integrating hepatitis B virus DNA, and produce hepatitis B virus continuously. Each of the cytokines inhibited h...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890470314
更新日期:1995-11-01 00:00:00
abstract::Clinical, virological, and histological findings in four HBsAg chronic carriers who cleared HBV markers from both serum and liver following HDV superinfection are described. The patients were long-term HBsAg carriers and all were HBV-DNA/HBeAg positive. Liver biopsy, obtained from three of the patients between 5 and 1...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310408
更新日期:1990-08-01 00:00:00
abstract::Many patients with coagulation disorders are infected with hepatitis C virus (HCV) that advances to end stage liver disease, resulting in an increased number of deaths. The efficacy of ribavirin and peginterferon combination therapy for chronic HCV infection in patients with coagulation disorders has not been clarifie...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23444
更新日期:2013-02-01 00:00:00
abstract::The effect on cellular (c) oncogene RNA levels was investigated after infection of permissive cells with cell culture adapted strains (AD-169, C-87, Davis) and unadapted clinical isolates (82-1, 84-2, 85-1) of human cytomegalovirus (HCMV). The results indicate that both adapted and unadapted strains of HCMV induce sub...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890340409
更新日期:1991-08-01 00:00:00
abstract::Physicochemical studies of hepatitis B e antigen (HBeAg) revealed a clear cut difference between e1 and e2 antigen. The e1 antigen was found to have a MW of Ca 150,000 and a pI of 6.4-7.2, whereas both the MW and pI of the e2 antigen were heterogeneous depending upon the source of serum. Sera obtained from asymptomati...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890040105
更新日期:1979-01-01 00:00:00
abstract::Hepatitis C virus (HCV) genotypes help to tailor the treatment response, but their influence on the disease severity and association with hepatic steatosis is not well understood. The prevalence of HCV genotypes and their correlation with the histopathological severity of liver disease and steatosis in Indian patients...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20561
更新日期:2006-04-01 00:00:00
abstract::The effectiveness of a daily continuous infusion of interferon-alpha was evaluated in 12 patients (10 males, 2 females; mean age of 33 years, range 19-62) with biopsy-proven chronic active hepatitis C. Nine million units (MU) of recombinant interferon-alpha 2A (rIFN-alpha 2A) were administered by continuous subcutaneo...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890370312
更新日期:1992-07-01 00:00:00
abstract::Evaluations of HIV-1 RNA viral load assays are lacking in Central Africa. The main objective of our study was to assess the reliability of HIV-1 RNA results obtained with three different assays for samples collected in Gabon. A total of 137 plasma specimens were assessed for HIV-1 RNA using the Abbott RealTime HIV-1® ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23770
更新日期:2014-01-01 00:00:00