Abstract:
:Co-infection with hepatitis delta virus (HDV) and hepatitis B virus (HBV) has been shown to be associated with a more severe form of acute and chronic hepatitis. Cloning and expression of recombinant HDV antigen (rHDAg) in Escherichiacoli are described. Using purified rHDAg, a cost-effective indirect anti-HDV enzyme-linked immunosorbent assay (ELISA) kit was developed. Direct comparison of 15 known HDV-positive sera and 15 HDV-negative sera showed concordance agreement between the new assay kit and the Abbott Murex Anti-Delta (total) kit. In addition, 1,486 hepatitis B surface antigen (HBsAg) positive blood samples collected from various areas of China were tested using this indirect anti-HDV ELISA. It was found that 1.2% (95% CI: 0.7-1.9%) of the samples were anti-HDAg positive. It is suggested that the prevalence of HDV and HBV co-infection in China is relatively low.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Shen L,Gu Y,Sun L,Yang Y,Wang F,Li Y,Bi Sdoi
10.1002/jmv.23212subject
Has Abstractpub_date
2012-03-01 00:00:00pages
445-9issue
3eissn
0146-6615issn
1096-9071journal_volume
84pub_type
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