Developmental expression of the IGF-II/mannose 6-phosphate receptor.

abstract：:In the sea urchin, some other marine invertebrates, and the frog, Xenopus, egg activation at fertilization is accompanied by an increase in intracellular pH (pHi). We measured pHi in germinal vesicle (GV)-intact mouse oocytes, ovulated eggs, and in vivo fertilized zygotes using the pH indicator dye, SNARF-1. The mean ...

journal_title：Molecular reproduction and development

authors： Phillips KP,Baltz JM

更新日期：1996-09-01 00:00:00

abstract：:Many studies have shown a correlation between transferrin (Tf) concentration and sperm yield in several mammalian species. We have used transgenic mice expressing human Tf (hTf) to investigate if overexpression of Tf increases the efficiency of mouse spermatogenesis. We demonstrated that a 36% increase of Tf does not ...

journal_title：Molecular reproduction and development

authors： Lécureuil C,Staub C,Fouchécourt S,Maurel MC,Fontaine I,Martinat N,Gauthier C,Daudignon A,Delaleu B,Sow A,Jégou B,Guillou F

更新日期：2007-02-01 00:00:00

abstract：:The fibrous sheath is a major cytoskeletal structure in the principal piece of the mammalian sperm flagellum. Two peptide sequences obtained from a tryptic digest of mouse fibrous sheath proteins exhibited high homology with mu-class glutathione S-transferases (GSTs). Using a DNA probe amplified from degenerate polyme...

journal_title：Molecular reproduction and development

authors： Fulcher KD,Welch JE,Klapper DG,O'Brien DA,Eddy EM

更新日期：1995-12-01 00:00:00

abstract：:We have tested three parameters in sperm-mediated gene transfer assays with mice and pigs: (i) the epididymal versus ejaculated origin of sperm cells, (ii) the primary structure, and (iii) the amount of the challenging foreign DNA. We have found that the pVLCNhGH construct, of retrotransposon origin, causes a massive ...

journal_title：Molecular reproduction and development

authors： Sciamanna I,Piccoli S,Barberi L,Zaccagnini G,Magnano AR,Giordano R,Campedelli P,Hodgson C,Lorenzini R,Spadafora C

更新日期：2000-06-01 00:00:00

abstract：:Tcp-11 is a candidate for a distorter gene within the t-complex on mouse chromosome 17; although t-complex genes appear to affect sperm function, relatively little is known about mechanisms whereby these genes might play a specific physiological role. We present evidence that the protein TCP-11 is found on the surface...

journal_title：Molecular reproduction and development

authors： Fraser LR,Hosseini R,Hanyalogou A,Talmor A,Dudley RK

更新日期：1997-11-01 00:00:00

abstract：:Using a specific protein binding assay we have shown that a spermatozoon of the ascidian Ciona intestinalis contains 1.58 +/- 0.74 x 10(-19) moles of inositol 1,4,5-tri-phosphate (InsP3), while a human spermatozoon contains 6.4 +/- 0.14 x 10(-19) moles. Induction of the acrosome reaction (AR) in both species, by expos...

journal_title：Molecular reproduction and development

authors： Tosti E,Palumbo A,Dale B

更新日期：1993-05-01 00:00:00

abstract：:In vitro oocyte maturation followed by in vitro fertilization (IVM/IVF) success in the domestic cat remains inferior to commonly studied livestock or laboratory species. The objectives here were (1) to histologically assess atresia status of freshly excised follicle/oocyte complexes, and (2) to evaluate taphonomic cha...

journal_title：Molecular reproduction and development

authors： Wood TC,Montali RJ,Wildt DE

更新日期：1997-02-01 00:00:00

abstract：:Three monoclonal antibodies against human protamines were obtained by immunization with total human basic nuclear proteins or purified protamine HP3. The specificity of antibodies was assessed by enzyme-linked immunosorbent assay (ELISA) and Western blot. They recognized three distinct epitopes: One was specific for t...

journal_title：Molecular reproduction and development

authors： Le Lannic G,Arkhis A,Vendrely E,Chevaillier P,Dadoune JP

更新日期：1993-09-01 00:00:00

abstract：:In general, maternal age affects the quality of oocytes and embryos. The present study aimed to examine the features and age-associated gene expression profiles of bovine oocytes and embryos as well as to discover possible countermeasures against age-associated events. Comprehensive gene expression assays of germinal ...

journal_title：Molecular reproduction and development

authors： Takeo S,Kawahara-Miki R,Goto H,Cao F,Kimura K,Monji Y,Kuwayama T,Iwata H

更新日期：2013-07-01 00:00:00

abstract：:It has been proposed that the viability of early mammalian embryos is associated with a metabolism that is "quiet" rather than "active" (Leese HJ, 2002:BioEssays 24:845-849). The data on which this hypothesis was based were largely drawn from measurements on the depletion and appearance of amino acids from the culture...

journal_title：Molecular reproduction and development

authors： Baumann CG,Morris DG,Sreenan JM,Leese HJ

更新日期：2007-10-01 00:00:00

abstract：:Three sources of hamster periovulatory fluids (+/- heat inactivation at 56 degrees C), with bovine serum albumin (BSA) as control, were tested for effects on penetration of three classes of eggs by hamster sperm precapacitated in BSA. These fluids were a soluble extract of cumulus oophorus fluid (COF) from the ovulate...

journal_title：Molecular reproduction and development

authors： Boatman DE,Magnoni GE,Robbins RS

更新日期：1994-08-01 00:00:00

abstract：:The internally fertilizing primitive frog Ascaphus truei (family Ascaphidae) from the Pacific Northwest is the only frog with an intromittent organ. The more advanced neobatrachian frog Eleutherodactylus coqui (family Leptodactylidae) from Puerto Rico has secondarily acquired internal fertilization but mates by cloaca...

journal_title：Molecular reproduction and development

authors： Kasinsky HE,Frehlick LJ,Su HW,Ausio J

更新日期：2005-03-01 00:00:00

abstract：:Mouse half-morulae were cryopreserved less than or equal to 1, 3, 6, and 12 hr after bisection by the vitrification method using 25% glycerol and 25% 1,2-propanediol as cryoprotectant. The developmental rates of the frozen-thawed half-embryos to blastocysts in vitro were 77.8% (63/81), 82.0% (41/50), 92.1% (117/127), ...

journal_title：Molecular reproduction and development

authors： Nagashima H,Kobayashi K,Yamakawa H,Ogawa S

更新日期：1991-11-01 00:00:00

abstract：:Production of transgenic cattle by microinjection of DNA has been difficult and costly. To explore an alternative method, one- to four-cell bovine embryos were exposed to a replication-defective retrovirus by microinjection of retrovirus producer cells into the perivitelline space. Embryos were cultured in vitro for 3...

journal_title：Molecular reproduction and development

authors： Haskell RE,Bowen RA

更新日期：1995-03-01 00:00:00

abstract：:Recently, mice and embryonic stem (ES) cells with allelic polymorphisms have been used extensively in the field of genetics and developmental biology. In this study, we examined whether intersubspecific hybrid mice and ES cells with these genotypes can be efficiently produced by intracytoplasmic sperm injection (ICSI)...

journal_title：Molecular reproduction and development

authors： Shinmen A,Honda A,Ohkawa M,Hirose M,Ogonuki N,Yuzuriha M,Miki H,Mochida K,Inoue K,Abe K,Ito M,Ogura A

更新日期：2007-09-01 00:00:00

abstract：:The aim of the present study is to investigate the effect of insulin-like growth factor (IGF-I) on the development of cultured rat pre-antral follicles. Pre-antral follicles with a diameter between 140 and 160 microm are mechanically isolated from ovaries of 10-day-old rats and cultured in groups for 6 days in FSH and...

journal_title：Molecular reproduction and development

authors： Zhao J,Taverne MA,Van Der Weijden GC,Bevers MM,Van Den Hurk R

更新日期：2001-03-01 00:00:00

abstract：:An antiserum, designated R4 and raised against denatured hamster acrosomes, was shown to localize specifically to the acrosomal region of hamster, rat, mouse, and human spermatozoa, and to inhibit both hamster and human sperm-oocyte binding in vitro. Following screening of a human testis lambda gt11 cDNA expression li...

journal_title：Molecular reproduction and development

authors： Moore A,Penfold LM,Johnson JL,Latchman DS,Moore HD

更新日期：1993-03-01 00:00:00

abstract：:Centrosome reduction during spermiogenesis has been studied using anti-gamma-tubulin and anti-centrin antibodies and electron microscopy in nonhuman primates. Rhesus spermatids possess apparently normal centrosomes comprising a pair of centrioles associated with gamma-tubulin and centrin. However, they do not nucleate...

journal_title：Molecular reproduction and development

authors： Manandhar G,Schatten G

更新日期：2000-08-01 00:00:00

abstract：:The objective of this study was to examine the effects of different culture media used for maturation of bovine oocytes on in vitro embryo development following in vitro fertilization. Oocytes were aspirated from 2-5 mm follicles of ovaries collected at a local abattoir. The oocyte-cumulus complexes (OCCs) were cultur...

journal_title：Molecular reproduction and development

authors： Rose TA,Bavister BD

更新日期：1992-01-01 00:00:00

abstract：:Prostaglandins could be involved in various aspects of final differentiation of ovarian follicles. Prostaglandins are generated by the cyclooxygenase (cox) pathway. Until now, the expression pattern of isoforms cox-1 and cox-2 of cyclooxygenase in bovine cumulus-oocyte complexes (COCs) was unknown. Using immunodetecti...

journal_title：Molecular reproduction and development

authors： Nuttinck F,Reinaud P,Tricoire H,Vigneron C,Peynot N,Mialot JP,Mermillod P,Charpigny G

更新日期：2002-01-01 00:00:00

abstract：:The Src family kinase (SFK) is important in normal cell cycle control. However, its role in meiotic maturation in mammalian has not been examined. We used confocal microscope immunofluorescence to examine the in vitro dynamics of the subcellular distribution of SFK during the mouse oocyte meiotic maturation and furthe...

journal_title：Molecular reproduction and development

authors： Zheng KG,Meng XQ,Yang Y,Yu YS,Liu DC,Li YL

更新日期：2007-01-01 00:00:00

abstract：:An in vitro superfusion method was used to test sex hormone release from different kinds of ovarian follicle (growing follicles, postovulatory follicles, and atretic follicles) in the lizard Podarcis sicula sicula. Sex hormone output changes with the stage of follicle evolution and sexual cycle. Previtellogenetic foll...

journal_title：Molecular reproduction and development

authors： Ciarcia G,Paolucci M,Di Fiore MM

更新日期：1993-07-01 00:00:00

abstract：:These experiments were designed to test the effects of an electrofusion and an electroporation pulse on bovine sperm-hamster egg development. In experiment 1, single motile sperm were injected into the perivitelline space of each egg. A 4,500 V/cm, 30 microseconds fusion pulse (FP) was applied while sperm-egg membrane...

journal_title：Molecular reproduction and development

authors： Rickords LF,White KL,Wiltbank JN

更新日期：1990-10-01 00:00:00

abstract：:In the cow, cryopreserved semen is inseminated into the uterus, and most of sperm are removed by backflow and phagocytes. Nevertheless, the mechanism responsible for sperm phagocytosis is unclear. Here, we used cultured bovine uterine epithelial cells (BUECs) to investigate the uterine response to sperm and the mechan...

journal_title：Molecular reproduction and development

authors： Elweza AE,Ezz MA,Acosta TJ,Talukder AK,Shimizu T,Hayakawa H,Shimada M,Imakawa K,Zaghloul AH,Miyamoto A

更新日期：2018-03-01 00:00:00

abstract：:Nuclear import of plasmid DNA mediated by a nuclear localization signal (NLS) derived from SV40 T antigen was investigated in a cell-free extract. In vitro assembled sea urchin male pronuclei were incubated in a 100,000g supernatant of a zebrafish fertilized egg lysate, together with fluorescently labeled plasmid DNA ...

journal_title：Molecular reproduction and development

authors： Collas P,Aleström P

更新日期：1996-12-01 00:00:00

abstract：:Proteins in the seminal fluid of animals with internal fertilization effect numerous responses in mated females that impact both male and female fertility. Among these proteins is the highly represented class of proteolysis regulators (proteases and their inhibitors). Though proteolysis regulators have now been identi...

journal_title：Molecular reproduction and development

authors： Laflamme BA,Wolfner MF

更新日期：2013-02-01 00:00:00

abstract：:A full-length cDNA encoding the bovine transforming growth factor beta (TGF-beta) receptor type I (bT beta R-I) was isolated from a placenta cDNA library. The deduced protein sequence of 499 residues contains a single transmembrane domain, a cysteinerich extracellular domain, and an intracellular kinase domain with pr...

journal_title：Molecular reproduction and development

authors： Roelen BA,Van Eijk MJ,Van Rooijen MA,Bevers MM,Larson JH,Lewin HA,Mummery CL

更新日期：1998-01-01 00:00:00

abstract：:cDNA clones encoding stage-and/or space-specific genes in porcine ovarian follicles have been investigated by differential screening of cDNA libraries of granulosa cells and corpus luteum. A clone corresponding to mRNA preferentially expressed in the functional stage of corpus luteum was found to encode a protein simi...

journal_title：Molecular reproduction and development

authors： Tanaka T,Itahana K,Andoh N,Takeya T,Sato E

更新日期：1995-10-01 00:00:00

abstract：:Mouse egg activation, which includes release from meiotic metaphase II arrest, results from fertilization-induced increase in intracellular calcium concentration ([Ca2+]i). However, during egg activation caused by exposure to the protein synthesis inhibitor, cycloheximide, [Ca2+]i did not change. Although eggs fertili...

journal_title：Molecular reproduction and development

authors： Moses RM,Kline D

更新日期：1995-06-01 00:00:00

abstract：:In this study, a combination of RNAi and endoscopic transfer to the oviduct of synchronized heifers has been used to investigate the effect of suppression of Cx43 and E-cadherin on the development, mRNA and protein expression of bovine blastocysts cultured in vitro or in vivo. In vitro matured and fertilized bovine zy...

journal_title：Molecular reproduction and development

authors： Tesfaye D,Lonergan P,Hoelker M,Rings F,Nganvongpanit K,Havlicek V,Besenfelder U,Jennen D,Tholen E,Schellander K

更新日期：2007-08-01 00:00:00