Abstract:
:We have tested three parameters in sperm-mediated gene transfer assays with mice and pigs: (i) the epididymal versus ejaculated origin of sperm cells, (ii) the primary structure, and (iii) the amount of the challenging foreign DNA. We have found that the pVLCNhGH construct, of retrotransposon origin, causes a massive embryo lethality and yet increases the yield of genetic transformation among born animals of both species compared to viral constructs. Arrest of embryonic development is a DNA dose-dependent effect, which is observed with high DNA doses, while lower doses are compatible with development. Finally, the overall efficiency of sperm-mediated gene transfer is higher when ejaculated, versus epididymal, spermatozoa are used. We suggest that this difference is related to the highly efficient apoptotic response in epididymal compared to ejaculated spermatozoa, triggered by the interaction of exogenous DNA molecules with the sperm membrane.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Sciamanna I,Piccoli S,Barberi L,Zaccagnini G,Magnano AR,Giordano R,Campedelli P,Hodgson C,Lorenzini R,Spadafora Cdoi
10.1002/(SICI)1098-2795(200006)56:2+<301::AID-MRD2keywords:
subject
Has Abstractpub_date
2000-06-01 00:00:00pages
301-5issue
2 Suppleissn
1040-452Xissn
1098-2795pii
10.1002/(SICI)1098-2795(200006)56:2+<301::AID-MRD2journal_volume
56pub_type
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