Abstract:
:A high efficient and simple transgenic technology on mice and rabbits to transfect spermatozoa with exogenous DNA/DMSO complex to obtain transgenic offspring, which is namely called DMSO-sperm mediated gene transfer (SMGT). Mouse sperm could be either directly transfected via injection into testis or cultured in vitro with the plasmed DNA containing the enhanced green fluorescent protein (EGFP) that could be expressed in the embryos and offspring. Then, 36 living transgenic rabbits were produced using the same technology, and the transgenic ratio of 56.3% was detected using PCR and Southern blot. As the controls, the transgenic ratios of 39.6% and 47.8% have also been tested using the liposomes mediated technology of Tfx-50 Reagent or Lipefectamin-2000, respectively. The results show that the female transgenic rabbits, as the mammary gland bioreactor models, could express the human tissue plasminogen activator mutant (htPAm) in their mammary cells when they are adult.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Shen W,Li L,Pan Q,Min L,Dong H,Deng Jdoi
10.1002/mrd.20401keywords:
subject
Has Abstractpub_date
2006-05-01 00:00:00pages
589-94issue
5eissn
1040-452Xissn
1098-2795journal_volume
73pub_type
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