Identification of a unique mu-class glutathione S-transferase in mouse spermatogenic cells.


:The fibrous sheath is a major cytoskeletal structure in the principal piece of the mammalian sperm flagellum. Two peptide sequences obtained from a tryptic digest of mouse fibrous sheath proteins exhibited high homology with mu-class glutathione S-transferases (GSTs). Using a DNA probe amplified from degenerate polymerase chain reaction (PCR) primers predicted from these two peptide sequences, a approximately 1.1 kb cDNA clone for fibrous sheath component 2 (Fsc2) was isolated which had 84% nucleic acid and 89% amino acid sequence identity with a previously reported mu-class human GST gene (hGSTM3; Campbell et al., 1990: J Biol Chem 265:4188-9193). Sequences corresponding to those of the two fibrous sheath peptides were present in the protein encoded by the Fsc2 cDNA. Northern analysis with the full length Fsc2 cDNA detected a approximately 1.1 kb mRNA in 12 of 15 somatic tissues examined, as well as in testis and isolated spermatogenic cells. However, 5'(nt--96 to 12) or 3'(nt 637 to 808) Fsc2 probes, containing mostly noncoding sequences, detected a approximately 1.1 kb mRNA abundant in testis and isolated spermatogenic cells, but absent or present at low levels in somatic tissues. Northern analysis with RNA from testes of mice of different postnatal ages and purified spermatogenic cell populations indicated that this transcript is first present during the meiotic phase of germ cell development. These results suggest that a previously unreported mu-class GST gene (mGSTM5.) is expressed at a specific time during the development of spermatogenic cells in the mouse. Immunoblot analysis indicated that a mu-class GST protein is associated with the fibrous sheath, suggesting that it becomes an integral part of the mouse sperm cytoskeleton.


Mol Reprod Dev


Fulcher KD,Welch JE,Klapper DG,O'Brien DA,Eddy EM




Has Abstract


1995-12-01 00:00:00












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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Arumugam K,Macnicol MC,Macnicol AM

    更新日期:2012-08-01 00:00:00

  • Red wine consumption may affect sperm biology: the effects of different concentrations of the phytoestrogen myricetin on human male gamete function.

    abstract::Myricetin is a natural flavonoid, particularly enriched in red wines, whose occurrence is widespread among plants. Despite extensive research, the beneficial effects of Myricetin on human health are still controversial. Here, we tested the estrogen-like effect of the phytoestrogen Myricetin on human ejaculated sperm b...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Aquila S,Santoro M,De Amicis F,Guido C,Bonofiglio D,Lanzino M,Cesario MG,Perrotta I,Sisci D,Morelli C

    更新日期:2013-02-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Kawase Y,Iwata T,Toyoda Y,Wakayama T,Yanagimachi R,Suzuki H

    更新日期:2001-09-01 00:00:00

  • Cleavage rate of haploid and diploid parthenogenetic mouse embryos during the preimplantation period.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Henery CC,Kaufman MH

    更新日期:1992-04-01 00:00:00

  • The effect of reactive oxygen species on motility parameters, DNA integrity, tyrosine phosphorylation and phosphatase activity of common carp (Cyprinus carpio L.) spermatozoa.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Gazo I,Shaliutina-Kolešová A,Dietrich MA,Linhartová P,Shaliutina O,Cosson J

    更新日期:2015-01-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Garcia MA,Meizel S

    更新日期:1996-12-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Sim BW,Cha JJ,Song BS,Kim JS,Yoon SB,Choi SA,Jeong KJ,Kim YH,Huh JW,Lee SR,Kim SH,Lee CS,Kim SU,Chang KT

    更新日期:2013-03-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Richoux V,Renard JP,Babinet C

    更新日期:1991-03-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Jones KL,King SS,Iqbal MJ

    更新日期:2004-02-01 00:00:00

  • Regulation of the ovine interferon-tau gene by a blastocyst-specific transcription factor, Cdx2.

    abstract::Expression of ovine interferon-tau (oIFNtau), a factor essential for the process of maternal recognition of pregnancy in ruminant ungulates, is restricted to the trophoblast. However, the molecular mechanisms by which oIFNtau expression is restricted to the trophectoderm have not been fully elucidated. The objective o...

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    pub_type: 杂志文章


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    更新日期:2006-05-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Feugang JM,Donnay I,Mermillod P,Marchandise J,Lequarre AS

    更新日期:2005-07-01 00:00:00

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    abstract::Mammalian reproductive processes involve spermatogenesis, which occurs in the testis, and fertilization, which takes place in the female genital tract. Fertilization is a successive, multistep, and extremely complicated event that usually includes sperm survival in the uterus, sperm migration through the uterotubal ju...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审


    authors: Xiong W,Wang Z,Shen C

    更新日期:2019-08-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Nagashima H,Kobayashi K,Yamakawa H,Ogawa S

    更新日期:1991-11-01 00:00:00

  • Early embryonic expression of the growth hormone family protein genes in the developing rainbow trout, Oncorhynchus mykiss.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Yang BY,Greene M,Chen TT

    更新日期:1999-06-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Dalbiès-Tran R,Papillier P,Pennetier S,Uzbekova S,Monget P

    更新日期:2005-08-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Gimenez-Bonafé P,Ribes E,Zamora MJ,Kasinsky HE,Chiva M

    更新日期:2002-07-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Lonergan P,Monaghan P,Rizos D,Boland MP,Gordon I

    更新日期:1994-01-01 00:00:00

  • Role of the major ecto-phosphoprotein in sperm flagellar motility using a cell electroporation method.

    abstract::Previous studies from our laboratory have identified MPS, a 100-kDa protein, as the major phosphoprotein substrate of caprine sperm ecto-cyclic AMP independent protein kinase. In this study the isolated (32)P-labelled MPS has been incorporated into mature caprine (Capra indicus) cauda-epididymal spermatozoa with the h...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Maiti A,Mishra KP,Majumder GC

    更新日期:2008-07-01 00:00:00

  • Effect of dibutyryl cAMP on the cAMP content, meiotic progression, and developmental potential of in vitro matured pre-pubertal and adult pig oocytes.

    abstract::Pre-pubertal pig oocytes display reduced developmental competence compared with adult oocytes following in vitro maturation (IVM). Exposure to dibutyryl cyclic adenosine monophosphate (dbcAMP) for the first 20 hr IVM improves development of pre-pubertal oocytes, suggesting that their cAMP content may be inadequate. Th...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Bagg MA,Nottle MB,Grupen CG,Armstrong DT

    更新日期:2006-10-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Lane M,Bavister BD

    更新日期:1999-12-01 00:00:00

  • Transient transmission of a transgene in mouse offspring following in vivo transfection of male germ cells.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Celebi C,Auvray P,Benvegnu T,Plusquellec D,Jégou B,Guillaudeux T

    更新日期:2002-08-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审


    authors: Baumann CG,Morris DG,Sreenan JM,Leese HJ

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  • The organic cation transporters (OCT1, OCT2, EMT) and the plasma membrane monoamine transporter (PMAT) show differential distribution and cyclic expression pattern in human endometrium and early pregnancy decidua.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Bottalico B,Noskova V,Pilka R,Larsson I,Domanski H,Casslén B,Hansson SR

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审


    authors: Jackowski S,Xu XX,Rock CO

    更新日期:1997-01-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Cohen J,Feldberg D

    更新日期:1991-09-01 00:00:00

  • Modulation of vascular cell behavior by transforming growth factors beta.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审


    authors: Madri JA,Bell L,Merwin JR

    更新日期:1992-06-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Ergenc AF,Li MW,Toner M,Biggers JD,Lloyd KC,Olgac N

    更新日期:2008-12-01 00:00:00

  • Reflections on E. E. Just, Black Apollo of Science, and the experiences of African American scientists.

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    journal_title:Molecular reproduction and development

    pub_type: 传,历史文章,杂志文章


    authors: Manning KR

    更新日期:2009-10-01 00:00:00

  • Sex differentiation and mRNA expression of P450c17, P450arom and AMH in gonads of the chicken.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Nishikimi H,Kansaku N,Saito N,Usami M,Ohno Y,Shimada K

    更新日期:2000-01-01 00:00:00

  • Presence and possible role of c-ras and nuclear (c-fos and c-jun) proto-oncogene products in preimplantation embryonic development in mice.

    abstract::The presence and possible role of products of nuclear (c-fos and c-jun) and c-ras proto-oncogenes were investigated in preimplantation embryonic development in mice. Polyclonal antibodies to c-fos or c-jun proto-oncogene products did not affect development of in vitro-cultured embryos from two-cell to morula or from m...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Ahmad K,Naz RK

    更新日期:1993-11-01 00:00:00