Identification of a unique mu-class glutathione S-transferase in mouse spermatogenic cells.


:The fibrous sheath is a major cytoskeletal structure in the principal piece of the mammalian sperm flagellum. Two peptide sequences obtained from a tryptic digest of mouse fibrous sheath proteins exhibited high homology with mu-class glutathione S-transferases (GSTs). Using a DNA probe amplified from degenerate polymerase chain reaction (PCR) primers predicted from these two peptide sequences, a approximately 1.1 kb cDNA clone for fibrous sheath component 2 (Fsc2) was isolated which had 84% nucleic acid and 89% amino acid sequence identity with a previously reported mu-class human GST gene (hGSTM3; Campbell et al., 1990: J Biol Chem 265:4188-9193). Sequences corresponding to those of the two fibrous sheath peptides were present in the protein encoded by the Fsc2 cDNA. Northern analysis with the full length Fsc2 cDNA detected a approximately 1.1 kb mRNA in 12 of 15 somatic tissues examined, as well as in testis and isolated spermatogenic cells. However, 5'(nt--96 to 12) or 3'(nt 637 to 808) Fsc2 probes, containing mostly noncoding sequences, detected a approximately 1.1 kb mRNA abundant in testis and isolated spermatogenic cells, but absent or present at low levels in somatic tissues. Northern analysis with RNA from testes of mice of different postnatal ages and purified spermatogenic cell populations indicated that this transcript is first present during the meiotic phase of germ cell development. These results suggest that a previously unreported mu-class GST gene (mGSTM5.) is expressed at a specific time during the development of spermatogenic cells in the mouse. Immunoblot analysis indicated that a mu-class GST protein is associated with the fibrous sheath, suggesting that it becomes an integral part of the mouse sperm cytoskeleton.


Mol Reprod Dev


Fulcher KD,Welch JE,Klapper DG,O'Brien DA,Eddy EM




Has Abstract


1995-12-01 00:00:00












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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Naito M,Sasaki E,Ohtaki M,Sakurai M

    更新日期:1994-02-01 00:00:00

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    journal_title:Molecular reproduction and development

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    authors: Zielak AE,Canty MJ,Forde N,Coussens PM,Smith GW,Lonergan P,Ireland JJ,Evans AC

    更新日期:2008-05-01 00:00:00

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    journal_title:Molecular reproduction and development

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    authors: Oliveira RL,Parent A,Cyr DG,Gregory M,Mandato CA,Smith CE,Hermo L

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Hunter RH,Petersen HH,Greve T

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    pub_type: 杂志文章,评审


    authors: Schatten H,Sun QY

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    pub_type: 杂志文章


    authors: Lane M,Bavister BD

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  • Differential expression of ornithine decarboxylase, poly(ADP)ribose polymerase, and mitochondrial mRNAs following testosterone administration to hypophysectomized rats.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Alcivar-Warren A,Trasler JM,Awoniyi CA,Zirkin BR,Hecht NB

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Sengupta A,Chakrabarti N,Sridaran R

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Tourte M,Besse C,Mounolou JC

    更新日期:1991-12-01 00:00:00

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Garcia MA,Meizel S

    更新日期:1996-12-01 00:00:00

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    journal_title:Molecular reproduction and development

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    authors: Parry RV,Barker PJ,Jones R

    更新日期:1992-09-01 00:00:00

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    authors: Kowalczyk AE,Kaczmarek MM,Schams D,Ziecik AJ

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    journal_title:Molecular reproduction and development

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Lozano JC,Schatt P,Vergé V,Gobinet J,Villey V,Peaucellier G

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Gazo I,Shaliutina-Kolešová A,Dietrich MA,Linhartová P,Shaliutina O,Cosson J

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审


    authors: LeRoith D,Kavsan VM,Koval AP,Roberts CT Jr

    更新日期:1993-08-01 00:00:00