Size-dependent isolation of primordial germ cells from avian species.

abstract：:To establish reliable criteria for the evaluation of nuclear donor embryos, we studied the effect of cell number and cell size of in vitro produced day 6 donor morulae on the rate of blastocyst formation following nuclear transfer to in vitro matured oocytes. In experiment 1, donor embryos were divided into three grou...

journal_title：Molecular reproduction and development

authors： Zakhartchenko V,Wolf E,Palma GA,Brem G

更新日期：1995-09-01 00:00:00

abstract：:Oocytes enucleated at metaphase II stage can support reprogramming of transferred nucleus and further developing to term. However, the first polar body in mice sometimes migrates away from the original place of expulsion, so the chromosomes of the oocyte will displace from the first polar body. Thus, it is not always ...

journal_title：Molecular reproduction and development

authors： Wang MK,Liu JL,Li GP,Lian L,Chen DY

更新日期：2001-04-01 00:00:00

abstract：:Demecolcine-induced enucleation (IE) of mouse oocytes has been shown to improve development to term of cloned mice. In this study, we characterized the kinetics and morphological progression of bovine oocytes subjected to IE, and evaluated their ability to support embryo development to the blastocyst stage after nucle...

journal_title：Molecular reproduction and development

authors： Russell DF,Ibáñez E,Albertini DF,Overström EW

更新日期：2005-10-01 00:00:00

abstract：:Various thiol compounds are known to improve cytoplasmic and/or nuclear maturation of oocytes in vitro. The present study examined the effects of two thiol compounds, cysteine (0.1, 0.5, and 1.0 mM) and cysteamine (50, 100, and 200 microM), on cytoplasmic and nuclear maturation of canine oocytes. Oocytes collected fro...

journal_title：Molecular reproduction and development

authors： Hossein MS,Kim MK,Jang G,Oh HJ,Koo O,Kim JJ,Kang SK,Lee BC,Hwang WS

更新日期：2007-09-01 00:00:00

abstract：:Fibulin-1 is a secreted protein associated with elastic matrix fibres and basement membranes. It plays a role in stabilizing blood vessels and can also regulate cell motility and invasiveness. We studied the regulation of the fibulin-1 gene in the rat and human endometrium, an organ where cyclic tissue remodeling and ...

journal_title：Molecular reproduction and development

authors： Haendler B,Yamanouchi H,Lessey BA,Chwalisz K,Hess-Stumpp H

更新日期：2004-07-01 00:00:00

abstract：:Progress in the understanding of early mammalian embryo development has been severely hampered by scarcity of study materials. To circumvent such a constraint, we have developed a strategy that involves a combination of in silico mining of new genes from expressed sequence tags (EST) databases and rapid determination ...

journal_title：Molecular reproduction and development

authors： Choo KB,Chen HH,Cheng WT,Chang HS,Wang M

更新日期：2001-07-01 00:00:00

abstract：:Homozygous deletion of the Lis1 gene (Lis1(-/-)) in mouse resulted in early embryonic lethality immediately after embryo implantation by an undefined mechanism. We seek to define the nature of this demise. LIS1 (pafah1b1) is a 46 kDa protein with seven tryptophan-aspartate (WD) repeats. It docks with many proteins and...

journal_title：Molecular reproduction and development

authors： Cahana A,Jin XL,Reiner O,Wynshaw-Boris A,O'Neill C

更新日期：2003-10-01 00:00:00

abstract：:Collagen is denatured in the gradual cervical ripening process during late pregnancy, already before the onset of final cervical ripening at parturition. Matrix Metallo Proteinases (MMPs) might be responsible for this process. To investigate the presence and potential function of MMPs at the different stages of the ri...

journal_title：Molecular reproduction and development

authors： van Engelen E,Breeveld-Dwarkasing VN,Taverne MA,Everts ME,van der Weijden GC,Rutten VP

更新日期：2008-11-01 00:00:00

abstract：:The homeobox-containing gene family plays a pivotal role in regulating, patterning, and axial morphogenesis in the developing embryo. But there is still very little known about the expression and function of these genes in mammalian oocytes and preimplantation stage embryos. In this study we have used degenerate prime...

journal_title：Molecular reproduction and development

authors： Ponsuksili S,Wimmers K,Adjaye J,Schellander K

更新日期：2001-11-01 00:00:00

abstract：:During early placental development, tumor suppressors and oncogenes work synergistically to regulate cell proliferation and differentiation in a restrained manner compared with the uncontrollable growth in cancer. One example of this partnership is the regulation of the oncofetal protein HMGA2 by BRCA1. BRCA1 forms a ...

journal_title：Molecular reproduction and development

authors： West RC,Russ JE,Bouma GJ,Winger QA

更新日期：2019-11-01 00:00:00

abstract：:The dysregulation of microRNAs (miRNAs) plays an important role in asthenozoospermia. This study evaluated the sperm microRNA-423-5p (miR-423-5p) expression in asthenozoospermia and normozoospermia, exploring the role of miR-423-5p in asthenozoospermia. Eighty participants were divided into asthenozoospermic (AZS, n =...

journal_title：Molecular reproduction and development

authors： Zhang R,Zuo Y,Cao S

更新日期：2021-01-28 00:00:00

abstract：:Altering incubation temperature during embryogenesis has an impact on chicken embryo growth, but the underlying molecular mechanisms are not understood; the present study was performed to address these changes. Broiler eggs were incubated at low (36.8°C), control (37.8°C), and high (38.8°C) temperatures between Embryo...

journal_title：Molecular reproduction and development

authors： Krischek C,Janisch S,Naraballobh W,Brunner R,Wimmers K,Wicke M

更新日期：2016-01-01 00:00:00

abstract：:Proteins in the seminal fluid of animals with internal fertilization effect numerous responses in mated females that impact both male and female fertility. Among these proteins is the highly represented class of proteolysis regulators (proteases and their inhibitors). Though proteolysis regulators have now been identi...

journal_title：Molecular reproduction and development

authors： Laflamme BA,Wolfner MF

更新日期：2013-02-01 00:00:00

abstract：:Successful pregnancy depends on the precise regulation of extravillous trophoblast cell invasion ability. MicroRNA-210-3p (miR-210), which is increased in the placenta of pre-eclampsia. Furthermore, miR-210 could inhibit trophoblasts invasion and might act as a serum biomarker for pre-eclampsia. Previous studies have ...

journal_title：Molecular reproduction and development

authors： Wang R,Liu W,Liu X,Liu X,Tao H,Wu D,Zhao Y,Zou L

更新日期：2019-07-01 00:00:00

abstract：:The influence of somatic cells on oocyte penetrability was studied during in vitro maturation. Four experiments were carried out. In the first, pig oocytes were fertilized in vitro immediately after collection (immature oocytes) or after being cultured for 44 hr with cumulus cells connected to the whole wall of the ex...

journal_title：Molecular reproduction and development

authors： Galeati G,Modina S,Lauria A,Mattioli M

更新日期：1991-05-01 00:00:00

abstract：:Insulators are located at the boundaries of differentially regulated genes and delimit their interactions by establishing independent chromatin structures. Recently, an insulator sequence has been found in the 5'-flanking region of arylsulfatase (ARS) gene from sea urchin. To investigate functional conservation of thi...

journal_title：Molecular reproduction and development

authors： Takada T,Iida K,Akasaka K,Yasue H,Torii R,Tsujimoto G,Taira M,Kimura H

更新日期：2000-11-01 00:00:00

abstract：:We aimed in this study to explore if sphingosine-1-phosphate (S1P) reduces apoptosis of primordial follicles during cryopreservation of human ovarian cortical samples. Ovarian cortical tissue fragments obtained from young patients who underwent laparoscopic excision of benign ovarian cysts were used for the experiment...

journal_title：Molecular reproduction and development

authors： Guzel Y,Bildik G,Dilege E,Oktem O

更新日期：2018-11-01 00:00:00

abstract：:Nuclear import of plasmid DNA mediated by a nuclear localization signal (NLS) derived from SV40 T antigen was investigated in a cell-free extract. In vitro assembled sea urchin male pronuclei were incubated in a 100,000g supernatant of a zebrafish fertilized egg lysate, together with fluorescently labeled plasmid DNA ...

journal_title：Molecular reproduction and development

authors： Collas P,Aleström P

更新日期：1996-12-01 00:00:00

abstract：:Myricetin is a natural flavonoid, particularly enriched in red wines, whose occurrence is widespread among plants. Despite extensive research, the beneficial effects of Myricetin on human health are still controversial. Here, we tested the estrogen-like effect of the phytoestrogen Myricetin on human ejaculated sperm b...

journal_title：Molecular reproduction and development

authors： Aquila S,Santoro M,De Amicis F,Guido C,Bonofiglio D,Lanzino M,Cesario MG,Perrotta I,Sisci D,Morelli C

更新日期：2013-02-01 00:00:00

abstract：:A quantitative primer extension method was used to measure the mass of histone gene transcripts in mature oocytes of the amphibian Xenopus laevis. The procedure, using a large excess of gene-specific oligonucleotide primer and continuous incorporation of a radiolabeled deoxynucleoside triphosphate precursor, is more s...

journal_title：Molecular reproduction and development

authors： Brashears-Macatee S,Hinkley C,Perry M

更新日期：1990-01-01 00:00:00

abstract：:Acrosomal reaction is an essential prerequisite to fertilization. The changes in lipid composition of sperm membranes cause fusion of the plasma and outer acrosomal membranes that results in the exocytosis of acrosomal contents. We report that both bull and rabbit spermatozoa contain a phosphatidylcholine-specific pho...

journal_title：Molecular reproduction and development

authors： Hinkovska-Galchev V,Srivastava PN

更新日期：1992-11-01 00:00:00

abstract：:While most cyclin-dependent kinases (CDKs) are involved in cell cycle control, CDK5 is mostly known for crucial functions in neurogenesis. However, we cloned sea urchin CDK5 from a two-cell stage cDNA library and found that the protein is present in eggs and embryos, up to the pluteus stage, but without associated kin...

journal_title：Molecular reproduction and development

authors： Lozano JC,Schatt P,Vergé V,Gobinet J,Villey V,Peaucellier G

更新日期：2010-05-01 00:00:00

abstract：:Three staining procedures to detect sperm acrosome integrity were compared via electron microscopy. Stains were applied to epididymal, freshly ejaculated, in vivo capacitated, and sonicated sperm cells in addition to spermatozoa displaying sequentially removed plasma and outer and inner acrosomal membranes. Sequential...

journal_title：Molecular reproduction and development

authors： McBride CE,Fayrer-Hosken RA,Srivastava PN,Brackett BG

更新日期：1990-05-01 00:00:00

abstract：:Infusion of seminal plasma in the uterus is known to elicit an instant inflammatory response in the porcine uterus, but whether or not it prepares a uterine immunological response to the presence of conceptuses is not well understood. Seminal plasma induced long-term modulatory effects and conceptus-induced immune cha...

journal_title：Molecular reproduction and development

authors： Jalali BM,Kitewska A,Wasielak M,Bodek G,Bogacki M

更新日期：2014-03-01 00:00:00

abstract：:Although placental transfer of maternal calcium (Ca(2+)) is a crucial process for fetal development, the biochemical mechanisms are not completely elucidated. Especially, mechanisms of syncytiotrophoblast Ca(2+) extrusion into fetal circulation remain to be established. In the current study we have investigated the ch...

journal_title：Molecular reproduction and development

authors： Moreau R,Daoud G,Masse A,Simoneau L,Lafond J

更新日期：2003-07-01 00:00:00

abstract：:Biochemical and immunoelectron microscopic methods have been used to analyze the distribution of actin in boar spermatozoa and its state of aggregation before and after acrosome reaction. F-actin was detected on sperm head and tail by electron microscopy using an improved phalloidin probe: incubation with a fluorescei...

journal_title：Molecular reproduction and development

authors： Castellani-Ceresa L,Brivio MF,Radaelli G

更新日期：1992-09-01 00:00:00

abstract：:It has been proposed that the viability of early mammalian embryos is associated with a metabolism that is "quiet" rather than "active" (Leese HJ, 2002:BioEssays 24:845-849). The data on which this hypothesis was based were largely drawn from measurements on the depletion and appearance of amino acids from the culture...

journal_title：Molecular reproduction and development

authors： Baumann CG,Morris DG,Sreenan JM,Leese HJ

更新日期：2007-10-01 00:00:00

abstract：:Previous studies from our laboratory have identified MPS, a 100-kDa protein, as the major phosphoprotein substrate of caprine sperm ecto-cyclic AMP independent protein kinase. In this study the isolated (32)P-labelled MPS has been incorporated into mature caprine (Capra indicus) cauda-epididymal spermatozoa with the h...

journal_title：Molecular reproduction and development

authors： Maiti A,Mishra KP,Majumder GC

更新日期：2008-07-01 00:00:00

abstract：:The formation of cell membrane following CSF-1 stimulation of a macrophage cell line is coordinated with cell cycle progression. The majority of membrane phospholipid accumulates during the S phase and results from cell-cycle dependent oscillations in the rates of phosphatidylcholine biosynthesis and degradation. Both...

journal_title：Molecular reproduction and development

authors： Jackowski S,Xu XX,Rock CO

更新日期：1997-01-01 00:00:00

abstract：:A method for assessment of the human sperm acrosome reaction is reported using fluorescein isothiocyanate (FITC)-conjugated Concanavalin A (ConA). The technique involved labelling prefixed spermatozoa, where only those spermatozoa that showed a complete loss of the acrosome bound FITC-ConA to the acrosomal region. Com...

journal_title：Molecular reproduction and development

authors： Holden CA,Hyne RV,Sathananthan AH,Trounson AO

更新日期：1990-03-01 00:00:00