Expression of homeobox-containing genes in cDNA libraries derived from cattle oocytes and preimplantation stage embryo.

Abstract:

:The homeobox-containing gene family plays a pivotal role in regulating, patterning, and axial morphogenesis in the developing embryo. But there is still very little known about the expression and function of these genes in mammalian oocytes and preimplantation stage embryos. In this study we have used degenerate primers corresponding to the highly conserved regions of Antennapedia class homeodomains as a rapid and an efficient method to survey bovine cDNA libraries derived from unfertilised oocytes, single 2-cell, 4-cell, 8-cell, morula, and blastocyst stage embryos for the presence of homeobox sequences. Our results provide, for the first time, evidence for the transcription of Hoxa3 and Hoxd1 in oocytes; Cdx1 and Cdx2 in the 2-cell; Cdx1, Hoxa1, Hoxd1, and Hoxd4 in the 4-cell; Cdx1, Hoxa1, and Hoxc9 in the 8-cell; Cdx2, Hoxb9, and Hoxc9 in the morula; Cdx2, Hoxb7, Hoxb9, and Hoxc9 in blastocyst stage cattle embryos. These are candidate genes for the developmental capacity of in vivo and in vitro produced bovine embryos.

journal_name

Mol Reprod Dev

authors

Ponsuksili S,Wimmers K,Adjaye J,Schellander K

doi

10.1002/mrd.1091

keywords:

subject

Has Abstract

pub_date

2001-11-01 00:00:00

pages

297-301

issue

3

eissn

1040-452X

issn

1098-2795

pii

10.1002/mrd.1091

journal_volume

60

pub_type

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