Abstract:
:Superovulated hamster oocytes were cryopreserved and thawed according to our carefully designed procedures. More than 90% (92 +/- 4%) of oocytes survived freezing and thawing. They were proven to be well conserved, showing excellent performance comparable to freshly ovulated oocytes in the human sperm penetration test (proportion of penetrated ova: 94.7% vs. 93.6%) and human sperm chromosome analysis (proportion of metaphasic ova: 81.8% vs. 83.6%). There was no statistically significant difference in the incidences of sperm chromosome aberrations between assays using fresh and frozen-thawed oocytes. In addition, there was no statistically significant increase of aberrations in female pronuclear (hamster) chromosomes. This freezing-thawing method was found to be reliable, yielding viable hamster oocytes of high quality.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Tateno H,Kamiguchi Y,Mikamo Kdoi
10.1002/mrd.1080330213keywords:
subject
Has Abstractpub_date
1992-10-01 00:00:00pages
202-9issue
2eissn
1040-452Xissn
1098-2795journal_volume
33pub_type
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journal_title:Molecular reproduction and development
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pub_type: 杂志文章
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pub_type: 杂志文章
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