Abstract:
:Altering incubation temperature during embryogenesis has an impact on chicken embryo growth, but the underlying molecular mechanisms are not understood; the present study was performed to address these changes. Broiler eggs were incubated at low (36.8°C), control (37.8°C), and high (38.8°C) temperatures between Embryonic Day (ED) 7 and 10 or ED 10 and 13, which cover critical periods of embryonic myogenesis. The embryos were then dissected immediately after treatment on ED 10 or 13 to assess body, liver, and heart weights as well as to analyze breast and leg muscle fibers for their mitochondrial respiratory activity (MRA). Breast muscle samples were additionally used to evaluate the activity of enzymes involved in energy metabolism and cell-cycle progression. ED-10 embryos incubated at 38.8°C showed elevated weights (body, liver, and heart), MRA, and activities of lactate dehydrogenase and cytochrome oxidase compared to the ED-10 embryos incubated at 36.8°C. Similarly, the ED-13 embryos incubated at 38.8°C showed elevated body weight, MRA, and activities of glycogen phosphorylase, phosphofructokinase, and cytochrome oxidase compared to their 36.8°C counterparts. Embryos incubated at the normal temperature (37.8°C), however, showed variable differences from those incubated at 38.8°C versus 36.8°C. Cell-cycle enzyme activities were not impacted by the different temperature treatments. Thus, an increase or decrease in the incubation temperature during embryonic broiler myogenesis results in altered embryo activity, muscle energy metabolism, and activity-dependent muscle growth.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Krischek C,Janisch S,Naraballobh W,Brunner R,Wimmers K,Wicke Mdoi
10.1002/mrd.22596subject
Has Abstractpub_date
2016-01-01 00:00:00pages
71-8issue
1eissn
1040-452Xissn
1098-2795journal_volume
83pub_type
杂志文章abstract::The cumulus cells that surround the mammalian oocyte become dispersed following the preovulatory surge of the pituitary gonadotropin, luteinizing hormone (LH). We have examined cumulus-oocyte complexes of PMSG-primed immature rats before and at 1, 2, 3, 4, 6, and 8 hr after injection of human chorionic gonadotropin (h...
journal_title:Molecular reproduction and development
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doi:10.1002/(SICI)1098-2795(199708)47:4<363::AID-MRD2>
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journal_title:Molecular reproduction and development
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更新日期:1994-05-01 00:00:00
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更新日期:1996-08-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10121
更新日期:2002-07-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20762
更新日期:2008-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 评论,杂志文章
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更新日期:2001-04-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420313
更新日期:1995-11-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080400306
更新日期:1995-03-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200001)55:1<104::AID-MRD14
更新日期:2000-01-01 00:00:00
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journal_title:Molecular reproduction and development
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doi:10.1002/mrd.21239
更新日期:2010-10-01 00:00:00
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journal_title:Molecular reproduction and development
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更新日期:2003-06-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330302
更新日期:1992-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00