The actions of in ovo cortisol on egg fertility, embryo development and the expression of growth-related genes in rainbow trout embryos, and the growth performance of juveniles.

abstract：:The genome of germline committed cells is thought to be protected by mechanisms of transcriptional silencing, posing a barrier to transgenesis using cultured germline cells. We found that selection for transgene integration into the primordial germ cell genome required that the transgenes be flanked by the chicken bet...

journal_title：Molecular reproduction and development

authors： Leighton PA,van de Lavoir MC,Diamond JH,Xia C,Etches RJ

更新日期：2008-07-01 00:00:00

abstract：:The objective of the present study was to investigate the ultrastructural consequences of vitrification of bovine oocytes at the metaphase II (MII) stage by the so-called "Open Pulled Straw" method. Oocytes were matured in vitro for 22 hr and cryopreserved by vitrification. After warming and additional 2 hr of culture...

journal_title：Molecular reproduction and development

authors： Hyttel P,Vajta G,Callesen H

更新日期：2000-05-01 00:00:00

abstract：:We have isolated and characterized a cDNA clone corresponding to a new member of bep (butanol, extracted, proteins) Paracentrotus lividus multigene family coding for cell surface proteins. The cDNA, called bep3, encodes a 370 amino acid protein and shares the same structural organization in the coding region with othe...

journal_title：Molecular reproduction and development

authors： Montana G,Romancino DP,di Carlo MD

更新日期：1996-05-01 00:00:00

abstract：:A novel gene Ggnbp1 was identified during yeast two-hybrid screening of gametogenetin protein 1 (GGN1)-interacting proteins. Ggnbp1 gene was found in mouse, rat, and human genomes but not in sequenced yeast, worms, fly, or fish genomes. Northern blotting analysis revealed that the gene was specifically expressed in th...

journal_title：Molecular reproduction and development

authors： Zhou Y,Zhao Q,Bishop CE,Huang P,Lu B

更新日期：2005-03-01 00:00:00

abstract：:The timing of the transition from maternal to zygotic control of development (MZT) and the initiation of transcription was studied in domestic cat embryos to determine if there is a temporal association between these phenomena and the development block observed in cat embryos fertilized in vitro. Embryos were derived ...

journal_title：Molecular reproduction and development

authors： Hoffert KA,Anderson GB,Wildt DE,Roth TL

更新日期：1997-10-01 00:00:00

abstract：:The aim of this study was to elucidate the relationship between protein tyrosine phosphorylation state and sperm characteristics in frozen-stored spermatozoa of Japanese Black bulls. The spermatozoa were washed with PBS containing polyvinyl alcohol and then incubated with cell-permeable cAMP analog cBiMPS to induce fl...

journal_title：Molecular reproduction and development

authors： Harayama H,Nishijima K,Murase T,Sakase M,Fukushima M

更新日期：2010-10-01 00:00:00

abstract：:Sry expression is essential for initiating male sex differentiation, and the expression occurs only during a restricted period in the developing gonad. It is thought that Sry is part of a pathway of genes that regulate sex determination. Although the interactions of several genes with Sry expression have been suggeste...

journal_title：Molecular reproduction and development

authors： Yokouchi K,Ito M,Nishino K,Yamanouchi K,Naito K,Suzawa M,Kato S,Hakamata Y,Endo H,Tojo H

更新日期：2003-04-01 00:00:00

abstract：:The red wolf (Canis rufus) is an endangered species with 194 individuals remaining in the wild and in various captive facilities. Breeding efforts at the Graham, WA site (Point Defiance Zoo and Aquarium) have involved artificial insemination with fresh or frozen semen in an effort to increase population and maximize t...

journal_title：Molecular reproduction and development

authors： Koehler JK,Platz CC Jr,Waddell W,Jones MH,Smith R,Behrns S

更新日期：1994-04-01 00:00:00

abstract：:Previously we demonstrated that lines of transgenic mice carrying the herpes simplex type 1 virus thymidine kinase (HSV1-tk) reporter gene are male-sterile. Ectopic transcription of the HSV1-tk reporter in the testis was initiated downstream of the normal translation initiation codon and truncated proteins consistent ...

journal_title：Molecular reproduction and development

authors： Ellison AR,Wallace H,al-Shawi R,Bishop JO

更新日期：1995-08-01 00:00:00

abstract：:Among Sicilian stick insects, two hybridogenetic complexes have been discovered: Bacillus rossius-grandii benazzii and B. rossius-grandii grandii, which also produce androgenetic offspring. The egg maturation of the former is analyzed here through DAPI fluorometry, which, besides the assessment of the meiotic stages, ...

journal_title：Molecular reproduction and development

authors： Tinti F,Scali V

更新日期：1992-11-01 00:00:00

abstract：:Exposed plasma membrane proteins were labeled with 125I before and after incubation of boar sperm under capacitating conditions. Labeled protein profiles were compared to the ability of the sperm to penetrate zona-free hamster ova. Quantitatively, the labeled sperm membrane proteins were primarily low Mr prior to capa...

journal_title：Molecular reproduction and development

authors： Berger T

更新日期：1990-11-01 00:00:00

abstract：:Inner cell masses (ICM) from in vitro produced day 8 or 9 bovine blastocysts were isolated by immunosurgery and cultured under different conditions in order to establish which of two feeder cell types and culture media were most efficient in supporting attachment and outgrowth of the bovine ICM cells. The efficiency o...

journal_title：Molecular reproduction and development

authors： Van Stekelenburg-Hamers AE,Van Achterberg TA,Rebel HG,Fléchon JE,Campbell KH,Weima SM,Mummery CL

更新日期：1995-04-01 00:00:00

abstract：:We have tested three parameters in sperm-mediated gene transfer assays with mice and pigs: (i) the epididymal versus ejaculated origin of sperm cells, (ii) the primary structure, and (iii) the amount of the challenging foreign DNA. We have found that the pVLCNhGH construct, of retrotransposon origin, causes a massive ...

journal_title：Molecular reproduction and development

authors： Sciamanna I,Piccoli S,Barberi L,Zaccagnini G,Magnano AR,Giordano R,Campedelli P,Hodgson C,Lorenzini R,Spadafora C

更新日期：2000-06-01 00:00:00

abstract：:Mitochondria play an important role in the integration and transmission of cell death signals mediated by the Bcl-2 family proteins. Experiments were conducted to determine whether the anti-apoptotic peptides BH4 domain of Bcl-xL (TAT-BH4) and Bax inhibitor peptide (BIP) suppresses heat stress (HS) injury in oocytes b...

journal_title：Molecular reproduction and development

authors： Soto P,Smith LC

更新日期：2009-07-01 00:00:00

abstract：:Communication between the oocyte and its somatic cells has been shown to be important in oocyte development. Here we examined how the oocyte may be involved in bovine cumulus cell expansion. Intact bovine cumulus oocyte complexes (COC) were obtained by puncturing antral follicles. From the intact COC, oocytectomised c...

journal_title：Molecular reproduction and development

authors： Ralph JH,Telfer EE,Wilmut I

更新日期：1995-10-01 00:00:00

abstract：:The spermatogonia of fish can be classified as being either undifferentiated type A spermatogonia or differentiated type B spermatogonia. Although type A spermatogonia, which contain spermatogonial stem cells, have been demonstrated to be a suitable material for germ cell transplantation, no molecular markers for dist...

journal_title：Molecular reproduction and development

authors： Yano A,von Schalburg K,Cooper G,Koop BF,Yoshizaki G

更新日期：2009-03-01 00:00:00

abstract：:Eggs of Ascidia ceratodes and Phallusia mammillata block polyspermy by releasing a phosphatidylinositol-linked glycosidase from the follicle cell and egg surface that binds to and blocks all unoccupied sperm binding sites on the vitelline coat. Release of this glycosidase is thought to be under the control of a membra...

journal_title：Molecular reproduction and development

authors： Robert LK,Lucio-Gough LM,Goode CA,Mckinney K,Lambert CC

更新日期：1999-09-01 00:00:00

abstract：:The effects of cryopreservation on the frequency and type of chromosomal abnormalities in human sperm were investigated. Employing a technique that enables direct visualization of human sperm chromosomes following in vitro penetration of hamster oocytes, sperm samples from 10 normal men were examined before and after ...

journal_title：Molecular reproduction and development

authors： Martin RH,Chernos JE,Rademaker AW

更新日期：1991-10-01 00:00:00

abstract：:Fully grown immature oocytes acquire the ability to be fertilized with sperm after meiotic maturation, which is finally accomplished by the formation and activation of the maturation-promoting factor (MPF). MPF is the complex of Cdc2 and cyclin B, and its function in promoting metaphase is common among species. The Mo...

journal_title：Molecular reproduction and development

authors： Yoshida N,Mita K,Yamashita M

更新日期：2000-09-01 00:00:00

abstract：:We report use of an in vitro assay (Barbato et al., 1998: Biol Reprod 58:686-699) to assess binding ability of cauda epididymal mouse sperm to a surrogate zona pellucida and effect of a synthetic peptide (Amann et al., 1999: J Androl 20: 42-46) on fertilization ability in in vitro fertilization (IVF) tests. Sperm from...

journal_title：Molecular reproduction and development

authors： Magargee SF,Cramer PG,Hammerstedt RH

更新日期：2000-12-01 00:00:00

abstract：:The present study investigated the effects of triiodothyronine (T3) on pituitary gonadotropin (GTH) subunits, thyroid stimulating hormone (TSH) β subunit, and growth hormone (GH) mRNA levels, as well as gonadal steroid secretion during different stages of reproduction in goldfish. Goldfish pituitary cells cultured wit...

journal_title：Molecular reproduction and development

authors： Allan ER,Habibi HR

更新日期：2012-09-01 00:00:00

abstract：:During early placental development, tumor suppressors and oncogenes work synergistically to regulate cell proliferation and differentiation in a restrained manner compared with the uncontrollable growth in cancer. One example of this partnership is the regulation of the oncofetal protein HMGA2 by BRCA1. BRCA1 forms a ...

journal_title：Molecular reproduction and development

authors： West RC,Russ JE,Bouma GJ,Winger QA

更新日期：2019-11-01 00:00:00

abstract：:Pannexins (Panxs) are channel-forming proteins that have homology to the invertebrate gap junction proteins, the innexins. These proteins form membrane channels implicated in ATP release. To evaluate the role of Panxs in the male reproductive tract, we investigated the distribution and regulation of Panx1 and 3 in the...

journal_title：Molecular reproduction and development

authors： Turmel P,Dufresne J,Hermo L,Smith CE,Penuela S,Laird DW,Cyr DG

更新日期：2011-02-01 00:00:00

abstract：:cAMP-dependent protein kinase A (PKA) plays important regulatory roles during mouse spermatogenesis. PKA-mediated signaling has been shown to regulate gene expression, chromatin condensation, capacitation, and motility during sperm development and behavior, although how PKA is regulated in spatiotemporal manners durin...

journal_title：Molecular reproduction and development

authors： Xu K,Qi H

更新日期：2014-07-01 00:00:00

abstract：:Embryonic stem (ES) cell lines are routinely derived from in vivo produced blastocysts. We investigated the efficiency of ES cells derivation from in vitro produced blastocysts either in monoculture or sequential culture. Zygotes from hybrid F1 B6D2 mice were cultured in vitro to the blastocyst stage in Potassium (K(+...

journal_title：Molecular reproduction and development

authors： Liu J,Schoonjans L,Tielens S,Speleman F,Cornelissen M,De Sutter P,Dhont M,Van der Elst J

更新日期：2006-08-01 00:00:00

abstract：:Homozygous deletion of the Lis1 gene (Lis1(-/-)) in mouse resulted in early embryonic lethality immediately after embryo implantation by an undefined mechanism. We seek to define the nature of this demise. LIS1 (pafah1b1) is a 46 kDa protein with seven tryptophan-aspartate (WD) repeats. It docks with many proteins and...

journal_title：Molecular reproduction and development

authors： Cahana A,Jin XL,Reiner O,Wynshaw-Boris A,O'Neill C

更新日期：2003-10-01 00:00:00

abstract：:Fragmentation occurs during early developmental stages of electrically activated oocytes and nuclear transfer (NT) embryos. It might contribute to the low developmental rate of porcine NT embryos. The present study was conducted to investigate whether the addition of sugars such as sorbitol or sucrose suppresses fragm...

journal_title：Molecular reproduction and development

authors： Im GS,Yang BS,Lai L,Liu Z,Hao Y,Prather RS

更新日期：2005-06-01 00:00:00

abstract：:The sequence of ionic changes involved in initiation of acrosomal exocytosis in capacitated mouse spermatozoa was investigated. Earlier studies demonstrated that a large influx of Na+ is required for exocytosis, this Na+ apparently being associated with an increase in intracellular pH (pHi) via an Na(+)-H+ exchanger. ...

journal_title：Molecular reproduction and development

authors： Fraser LR

更新日期：1993-11-01 00:00:00

abstract：:An in vitro superfusion method was used to test sex hormone release from different kinds of ovarian follicle (growing follicles, postovulatory follicles, and atretic follicles) in the lizard Podarcis sicula sicula. Sex hormone output changes with the stage of follicle evolution and sexual cycle. Previtellogenetic foll...

journal_title：Molecular reproduction and development

authors： Ciarcia G,Paolucci M,Di Fiore MM

更新日期：1993-07-01 00:00:00

abstract：:Varicocele is a prevalent pathology among infertile men. The mechanisms linking this condition to infertility, however, are poorly understood. Our previous work showed a relationship between sperm functional quality and the ability of spermatozoa to respond to capacitating conditions with increased membrane fluidity a...

journal_title：Molecular reproduction and development

authors： Buffone MG,Brugo-Olmedo S,Calamera JC,Verstraeten SV,Urrutia F,Grippo L,Corbetta JP,Doncel GF

更新日期：2006-12-01 00:00:00