Abstract：
:Inner cell masses (ICM) from in vitro produced day 8 or 9 bovine blastocysts were isolated by immunosurgery and cultured under different conditions in order to establish which of two feeder cell types and culture media were most efficient in supporting attachment and outgrowth of the bovine ICM cells. The efficiency of attachment and outgrowth of the ICM cells could be markedly improved when STO feeder cells were used instead of bovine uterus epithelial cells, and by using charcoal-stripped serum instead of normal serum to supplement the culture medium. More than 20 stable cell lines were obtained. Some of these lines were examined by immunofluorescence for developmentally regulated markers. From these results we conclude that the cell lines resemble epithelial cells, rather than pluripotent ICM cells. The developmental potential of cells of one of the lines was tested in the nuclear transfer assay. The cell line could support the initial development of enucleated oocytes, but none of the reconstructed embryos passed the eight-cell block.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Van Stekelenburg-Hamers AE,Van Achterberg TA,Rebel HG,Fléchon JE,Campbell KH,Weima SM,Mummery CLdoi
10.1002/mrd.1080400408subject
Has Abstractpub_date
1995-04-01 00:00:00pages
444-54issue
4eissn
1040-452Xissn
1098-2795journal_volume
40pub_type
杂志文章abstract：:The first indication that the insulin-like growth factor-II/mannose 6-phosphate receptor (IGF-II/M6PR) is developmentally regulated came from studies of the serum form of the receptor in the rat. By immunoblotting, the circulating form of the receptor, which was 10 kDa smaller than the tissue receptor, was high in 19 ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.1080350415
更新日期：1993-08-01 00:00:00
abstract：:Many methods for efficient production of transgenic animals for biomedical research have been developed. Despite great improvements in transgenesis rates resulting from the use of intracytoplasmic sperm injection (ICSI), the ICSI-based sperm-mediated gene-transfer (iSMGT) technique is still not optimal in terms of spe...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22158
更新日期：2013-03-01 00:00:00
abstract：:The aims of this study were to investigate steady-state level of Kit Ligand (KL) mRNA and its effects on in vitro survival and growth of caprine preantral follicles. RT-PCR was used to analyze caprine steady-state level of KL mRNA in primordial, primary, and secondary follicles, and in small (1-3 mm) and large (3-6 mm...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21138
更新日期：2010-03-01 00:00:00
abstract：:We have tested three parameters in sperm-mediated gene transfer assays with mice and pigs: (i) the epididymal versus ejaculated origin of sperm cells, (ii) the primary structure, and (iii) the amount of the challenging foreign DNA. We have found that the pVLCNhGH construct, of retrotransposon origin, causes a massive ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(200006)56:2+<301::AID-MRD2
更新日期：2000-06-01 00:00:00
abstract：:Centrosome reduction during spermiogenesis has been studied using anti-gamma-tubulin and anti-centrin antibodies and electron microscopy in nonhuman primates. Rhesus spermatids possess apparently normal centrosomes comprising a pair of centrioles associated with gamma-tubulin and centrin. However, they do not nucleate...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/1098-2795(200008)56:4<502::AID-MRD8>3.0.CO
更新日期：2000-08-01 00:00:00
abstract：:In this study we have examined the effect of insulin like growth factor I (IGF-I) and its interaction with gonadotropins in the presence or absence of granulosa cell coculture on in vitro oocyte maturation (IVM) and their subsequent embryonic development in buffalo. We also have examined the role of IGF-I alone or in ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199803)49:3<277::AID-MRD8>
更新日期：1998-03-01 00:00:00
abstract：:While most cyclin-dependent kinases (CDKs) are involved in cell cycle control, CDK5 is mostly known for crucial functions in neurogenesis. However, we cloned sea urchin CDK5 from a two-cell stage cDNA library and found that the protein is present in eggs and embryos, up to the pluteus stage, but without associated kin...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21165
更新日期：2010-05-01 00:00:00
abstract：:Formation and maintenance of testis cords during embryogenesis are essential for establishing testicular structure and function in adults. At least five genes (Wt1, Dhh, Sox8/Sox9, and Dax1) appear to be required for the maintenance of testis cord integrity in mice. Here, we report that RFX1 is specifically expressed ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22660
更新日期：2016-07-01 00:00:00
abstract：:Glycogen synthase kinase-3 (GSK-3) is a highly conserved serine/threonine protein kinase implicated in diverse cellular processes. Activity of GSK-3 is essential for meiotic chromatin segregation in oocytes, yet expression and/or function of GSK-3 have not been reported in mammalian preimplantation embryos. Objectives...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20495
更新日期：2007-02-01 00:00:00
abstract：:The role of extracellular matrix (ECM) in the differentiation of tissue types was examined in embryos of Strongylocentrotus purpuratus. We have examined the expression of various tissue-specific molecular markers after disrupting the ECM by culturing embryos in the presence of beta-aminoproprionitrile fumarate (BAPN),...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080290303
更新日期：1991-07-01 00:00:00
abstract：:The gonadotropin releasing hormone (GnRH) has long been considered as a neuropeptide involved in the control of the reproductive cycle. However, the presence of GnRH and its receptors in various tissues, including ovary and testis, suggests a role as autocrine/paracrine factor. In the present study, we report the expr...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1000
更新日期：2001-05-01 00:00:00
abstract：:In this study, we sought to determine the mechanism of early sex reversal in a teleost by applying 4 hr feminization treatments to XY (17α-ethynylestradiol 2000 μg L(-1) ) and YY (6500 μg L(-1) ) Nile tilapia embryos on the first day post-fertilization (dpf). We then searched for changes in the expression profiles of ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22436
更新日期：2014-12-01 00:00:00
abstract：:Three staining procedures to detect sperm acrosome integrity were compared via electron microscopy. Stains were applied to epididymal, freshly ejaculated, in vivo capacitated, and sonicated sperm cells in addition to spermatozoa displaying sequentially removed plasma and outer and inner acrosomal membranes. Sequential...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080260106
更新日期：1990-05-01 00:00:00
abstract：:The oviduct is a dynamic organ which modulates gamete physiology. Sperm-oviduct interaction provides the formation of a sperm storage reservoir and allows the selection of sperm with certain qualities in eutherian mammals. In sows, the oviductal sperm binding glycoprotein (SBG) has been proposed to be involved in sper...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20958
更新日期：2009-04-01 00:00:00
abstract：:During bovine embryogenesis, bovine growth hormone (bGH) contributes to proliferation, differentiation, and modulation of embryo metabolism. Pituitary-specific transcription factor-1 (Pit-1) is a transcription factor that binds to promoters of GH, prolactin (PRL), and thyroid-stimulating hormone-beta (TSHbeta) encodin...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10237
更新日期：2003-03-01 00:00:00
abstract：:Testis induction is associated with gonadal Sry and Sox9 expression in mammals, and with Sox9 expression in vertebrates where Sry is absent. In mammals, Sry might initiate testis induction by upregulating Sox9 expression; however, direct evidence supporting this hypothesis is lacking. Models of Sry-negative XX sex rev...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10317
更新日期：2003-08-01 00:00:00
abstract：:These experiments were designed to test the effects of an electrofusion and an electroporation pulse on bovine sperm-hamster egg development. In experiment 1, single motile sperm were injected into the perivitelline space of each egg. A 4,500 V/cm, 30 microseconds fusion pulse (FP) was applied while sperm-egg membrane...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080270212
更新日期：1990-10-01 00:00:00
abstract：:Increased oxidative stress is one of the main causes of poorly developed embryos in assisted reproductive technologies. Nicotinamide (NAM) has been shown to suppress reactive oxygen species (ROS) production through its potent antioxidative and anti-senescent effects. In the present study, we explored the effects of sh...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23417
更新日期：2020-10-01 00:00:00
abstract：:Enzyme activities were determined quantitatively in individual rat oocytes to study their energy metabolism during maturation. Low hexokinase activity and high activities of lactate dehydrogenase and enzymes in the phosphate pathway, i.e., glucose 6-P and 6-P gluconate dehydrogenases, were characteristic of immature o...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080330315
更新日期：1992-11-01 00:00:00
abstract：:Specification of primordial germ cells during early embryogenesis is a critical biological issue in reproduction and development. Yet, little is known in marine economic fish species. Vasa, a component of germ plasm, is the most-documented germ cell marker in teleosts. We isolated a full-length vasa cDNA (Smvas) from ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22120
更新日期：2012-11-01 00:00:00
abstract：:To study possible role and regulation of apoptosis occurred in primate endometrium, the expression of apoptosis-related molecules, Fas, FasL, B cell lymphoma/leukaemia-2 (Bcl-2), and Bax were analyzed in relation to occurrence of apoptosis and proliferation in the cycling endometrium of the rhesus monkey using immunoh...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20215
更新日期：2005-04-01 00:00:00
abstract：:Connexin 43 (Cx43), a gap junction protein expressed in differentiated granulosa cells, is necessary for normal follicular development. Cx43 expression and regulation by epidermal growth factor (EGF) were characterized in immature rabbit granulosa cells. Cx43 mRNA was expressed in the granulosa cells of primary follic...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10219
更新日期：2003-01-01 00:00:00
abstract：:Proteins in the seminal fluid of animals with internal fertilization effect numerous responses in mated females that impact both male and female fertility. Among these proteins is the highly represented class of proteolysis regulators (proteases and their inhibitors). Though proteolysis regulators have now been identi...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.22130
更新日期：2013-02-01 00:00:00
abstract：:Mouse egg activation, which includes release from meiotic metaphase II arrest, results from fertilization-induced increase in intracellular calcium concentration ([Ca2+]i). However, during egg activation caused by exposure to the protein synthesis inhibitor, cycloheximide, [Ca2+]i did not change. Although eggs fertili...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080410218
更新日期：1995-06-01 00:00:00
abstract：:The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22425
更新日期：2014-12-01 00:00:00
abstract：:The present study examined the effect of elevated Ca(2+) concentration in fusion/activation medium on the fusion and development of fetal fibroblast nuclear transfer (NT) porcine embryos. Frozen-thawed and serum starved fetal fibroblasts were transferred into the perivitelline space of enucleated oocytes. Cell fusion ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10110
更新日期：2002-04-01 00:00:00
abstract：:Remodeling and relaxation of the mouse pubic symphysis (PS) are central events in parturition. The mouse PS remodels in a hormone-controlled process that involves the modification of the fibrocartilage into an interpubic ligament (IpL), followed by its relaxation prior to parturition. It is recognized that nitric oxid...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22020
更新日期：2012-04-01 00:00:00
abstract：:This study was carried out to examine the participation of epidermal growth factor (EGF)-like peptides in the induction of germinal vesicle breakdown (GVB) in mouse cumulus cell-enclosed oocytes (CEO). The EGF-like peptide, amphiregulin (AR), dose-dependently stimulated meiotic resumption in CEO, but not denuded oocyt...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20781
更新日期：2008-01-01 00:00:00
abstract：:Sperm-mediated gene transfer in vertebrates has undergone various developments over the last few years, in different laboratories. In the present study, we microinjected a circular plasmid, carrying the lacZ reporter gene mixed with noncommercial cationic lipids, into the seminiferous tubules of anesthetized adult mic...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10143
更新日期：2002-08-01 00:00:00
abstract：:The murine intracisternal A particle (IAP) proviral elements are expressed at low levels in undifferentiated F9 embryonal carcinoma cells but are highly expressed when F9 cells are induced to differentiate into parietal endoderm-like cells. IAP elements are also expressed in parietal endoderm-like PYS-2 cells. We prev...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080410103
更新日期：1995-05-01 00:00:00
