Abstract:
:Testis induction is associated with gonadal Sry and Sox9 expression in mammals, and with Sox9 expression in vertebrates where Sry is absent. In mammals, Sry might initiate testis induction by upregulating Sox9 expression; however, direct evidence supporting this hypothesis is lacking. Models of Sry-negative XX sex reversal (XXSR), in which testes develop in the absence of Sry, could provide the link between Sry and Sox9 in testis induction. To define the stages at which testis determination occurs in the canine model, Sry and Sox9 expression were measured in normal urogenital ridges (UGR) and gonads by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Testicular Sry expression rose continuously during canine developmental ages comparable to human carnegie stages (CS) 16-18, with maximal expression at CS 18. Sox9 was expressed in both male and female canine UGR up to CS 17, at which time testis expression became tenfold greater than in the ovary. Although Sox9 was detected by qRT-PCR in ovaries and mesonephroi of both sexes, expression was detected only in canine testes by whole mount in situ hybridization (WMISH). The timing of Sry and Sox9 expression is consistent with a role in testis determination: Sry expression begins at CS 16 in testes, followed by upregulation of Sox9 expression at CS 17. The quantity and temporal and spatial patterns of Sry and Sox9 expression in normal canine gonads are similar to those in humans, sheep, and pigs. These studies should provide the basis for understanding the mechanism of testis induction in the canine model of Sry-negative XXSR.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Meyers-Wallen VNdoi
10.1002/mrd.10317keywords:
subject
Has Abstractpub_date
2003-08-01 00:00:00pages
373-81issue
4eissn
1040-452Xissn
1098-2795journal_volume
65pub_type
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更新日期:2011-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-09-01 00:00:00
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更新日期:2008-03-01 00:00:00
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更新日期:2017-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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