Abstract：
:In vitro studies have suggested that the Cables1 gene regulates epithelial cell proliferation, whereas other studies suggest a role in promoting neural differentiation. In efforts to clarify the functions of Cables1 in vivo, we conducted gain- and loss-of-function studies targeting its ortholog (cables1) in the zebrafish embryo. Similar to rodents, zebrafish cables1 mRNA expression is detected most robustly in embryonic neural tissues. Antisense knockdown of cables1 leads to increased numbers of apoptotic cells, particularly in brain tissue, in addition to a distinct behavioral phenotype, characterized by hyperactivity in response to stimulation. Apoptosis and the behavioral abnormality could be rescued by co-expression of a morpholino-resistant cables1 construct. Suppression of p53 expression in cables1 morphants partially rescued both apoptosis and the behavioral phenotype, suggesting that the phenotype of cables1 morphants is due in part to p53-dependent apoptosis. Alterations in the expression patterns of several neural transcription factors were observed in cables1 morphants during early neurulation, suggesting that cables1 is required for early neural differentiation. Ectopic overexpression of cables1 strongly disrupted embryonic morphogenesis, while overexpression of a cables1 mutant lacking the C-terminal cyclin box had little effect, suggesting functional importance of the cyclin box. Lastly, marked reductions in p35, but not Cdk5, were observed in cables1 morphants. Collectively, these data suggest that cables1 is important for neural differentiation during embryogenesis, in a mechanism that likely involves interactions with the Cdk5/p35 kinase pathway.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Groeneweg JW,White YA,Kokel D,Peterson RT,Zukerberg LR,Berin I,Rueda BR,Wood AWdoi
10.1002/mrd.21263subject
Has Abstractpub_date
2011-01-01 00:00:00pages
22-32issue
1eissn
1040-452Xissn
1098-2795journal_volume
78pub_type
杂志文章abstract：:The reproductive tract environment at conception programs the developmental trajectory of the embryo, sets the course of pregnancy, and impacts offspring phenotype and health. Despite the fundamental importance of this stage of reproduction, the rate-limiting regulatory mechanisms operating locally to control fertilit...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.22823
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abstract：:A method for assessment of the human sperm acrosome reaction is reported using fluorescein isothiocyanate (FITC)-conjugated Concanavalin A (ConA). The technique involved labelling prefixed spermatozoa, where only those spermatozoa that showed a complete loss of the acrosome bound FITC-ConA to the acrosomal region. Com...
journal_title：Molecular reproduction and development
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doi：10.1002/mrd.1080250306
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abstract：:Testis induction is associated with gonadal Sry and Sox9 expression in mammals, and with Sox9 expression in vertebrates where Sry is absent. In mammals, Sry might initiate testis induction by upregulating Sox9 expression; however, direct evidence supporting this hypothesis is lacking. Models of Sry-negative XX sex rev...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10317
更新日期：2003-08-01 00:00:00
abstract：:Previous studies from our laboratory have identified MPS, a 100-kDa protein, as the major phosphoprotein substrate of caprine sperm ecto-cyclic AMP independent protein kinase. In this study the isolated (32)P-labelled MPS has been incorporated into mature caprine (Capra indicus) cauda-epididymal spermatozoa with the h...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20854
更新日期：2008-07-01 00:00:00
abstract：:Basigin, a transmembrane glycoprotein belonging to the immunoglobulin superfamily, has been shown to be essential for fertilization and implantation. The aim of this study was to determine the expression and hormonal regulation of basigin gene in mouse uterus during the peri-implantation period. Basigin immunostaining...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10128
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20728
更新日期：2007-11-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20523
更新日期：2007-02-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22808
更新日期：2017-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(200005)56:1<80::AID-MRD10>
更新日期：2000-05-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080410405
更新日期：1995-08-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080320404
更新日期：1992-08-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1050
更新日期：2001-08-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/1098-2795(200010)57:2<109::AID-MRD1>3.0.CO
更新日期：2000-10-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199807)50:3<334::AID-MRD10
更新日期：1998-07-01 00:00:00
abstract：:Topoisomerase II (topo II) is an essential enzyme that alters DNA topology. This activity is important for a variety of chromosome functions including DNA replication, transcription, recombination, and chromosome condensation and segregation. Previously we localized topo II in mouse gametes and preimplantation embryos...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10015
更新日期：2002-03-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20781
更新日期：2008-01-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080400306
更新日期：1995-03-01 00:00:00
abstract：:Changes in the level of transcriptional activity in 32-cell stage morula nuclei were studied after blastomere electrofusion to enucleated oocytes. Nuclear transplant recipients were pulse labelled with 3H-uridine during cultivation in vitro, embryos were then fixed and processed for autoradiography and electron micros...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199602)43:2<135::AID-MRD1>
更新日期：1996-02-01 00:00:00
abstract：:Annexins are a family of Ca(2+)-binding proteins involved in the exocytotic process. The presence and the role of annexins in mammalian spermatozoa have not been well established. Two annexin-like proteins were obtained from guinea pig testis, a doublet of Mr 31-33 kD (p31/33) and a protein of Mr 50 kD (p50). Both pro...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199603)43:3<366::AID-MRD11
更新日期：1996-03-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1018
更新日期：2001-06-01 00:00:00
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doi：10.1002/(SICI)1098-2795(199907)53:3<294::AID-MRD5>
更新日期：1999-07-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21233
更新日期：2010-10-01 00:00:00
abstract：:Although the epididymal environment promotes the maturation and survival of spermatozoa, not all spermatozoa remain viable during passage through the epididymis. Does the epididymis has a protective mechanism(s) to segregate the viable sperm from defective spermatozoa? Previously, we identified 260/280 kDa oligomers (...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23438
更新日期：2020-12-01 00:00:00
abstract：:The internally fertilizing primitive frog Ascaphus truei (family Ascaphidae) from the Pacific Northwest is the only frog with an intromittent organ. The more advanced neobatrachian frog Eleutherodactylus coqui (family Leptodactylidae) from Puerto Rico has secondarily acquired internal fertilization but mates by cloaca...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20207
更新日期：2005-03-01 00:00:00
abstract：:Progesterone (P) has previously been shown to rapidly increase free intracellular calcium concentration ([Ca2+]i), and subsequently to initiate the acrosome reaction (AR) in capacitated human sperm. The present study used cytochemical analysis of the AR, and spectrofluorometric determination of sperm [Ca2+]i and intra...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199612)45:4<513::AID-MRD14
更新日期：1996-12-01 00:00:00
abstract：:Hyperactivated motility was studied in guinea pig spermatozoa. In the presence of the local anesthetic procaine, a high number of sperm cells (64%) showed hyperactivation when incubated in minimal culture medium with pyruvate, lactate, and glucose. Hyperactivated motility was dependent on glucose in the medium. Sperm ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080380309
更新日期：1994-07-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199709)48:1<45::AID-MRD6>3
更新日期：1997-09-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080300309
更新日期：1991-11-01 00:00:00
abstract：:The murine intracisternal A particle (IAP) proviral elements are expressed at low levels in undifferentiated F9 embryonal carcinoma cells but are highly expressed when F9 cells are induced to differentiate into parietal endoderm-like cells. IAP elements are also expressed in parietal endoderm-like PYS-2 cells. We prev...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080410103
更新日期：1995-05-01 00:00:00
abstract：:The sequence of ionic changes involved in initiation of acrosomal exocytosis in capacitated mouse spermatozoa was investigated. Earlier studies demonstrated that a large influx of Na+ is required for exocytosis, this Na+ apparently being associated with an increase in intracellular pH (pHi) via an Na(+)-H+ exchanger. ...
journal_title：Molecular reproduction and development
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doi：10.1002/mrd.1080360313
更新日期：1993-11-01 00:00:00
