Abstract:
:Basigin, a transmembrane glycoprotein belonging to the immunoglobulin superfamily, has been shown to be essential for fertilization and implantation. The aim of this study was to determine the expression and hormonal regulation of basigin gene in mouse uterus during the peri-implantation period. Basigin immunostaining and mRNA were strongly localized in luminal and glandular epithelium on day 1 of pregnancy and gradually decreased to a basal level from day 2-4 of pregnancy. Basigin mRNA expression in the sub-luminal stroma was first detected on day 3 of pregnancy and increased on day 4 of pregnancy. On day 5 of pregnancy, the expression of basigin protein and mRNA was only detected in the implanting embryos, and the luminal epithelium and sub-luminal stroma surrounding the embryos. A similar expression pattern of basigin was also induced in the delayed-implantation uterus which was activated by estrogen injection. On day 6-8 of pregnancy, although a basal level of basigin protein was detected in the secondary decidual zone, basigin mRNA expression was strongly seen in this location. Basigin mRNA was also highly expressed in the decidualized cells under artificial decidualization. Estrogen significantly stimulated basigin expression in the ovariectomized mouse uterus. A high level of basigin immunostaining and mRNA was also seen in proestrus and estrus uteri. These results suggest that basigin expression is closely related to mouse implantation and up-regulated by estrogen.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Xiao LJ,Chang H,Ding NZ,Ni H,Kadomatsu K,Yang ZMdoi
10.1002/mrd.10128keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
47-54issue
1eissn
1040-452Xissn
1098-2795journal_volume
63pub_type
杂志文章abstract::Fertilization causes mature oocytes or eggs to increase their concentrations of intracellular calcium ions (Ca²⁺) in all animals that have been examined, and such Ca²⁺ elevations, in turn, provide key activating signals that are required for non-parthenogenetic development. Several lines of evidence indicate that the ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.22222
更新日期:2013-10-01 00:00:00
abstract::Follicle-stimulating hormone (FSH) regulates folliculogenesis in the ovary and spermatogenesis in the testis via specific, high affinity membrane-bound receptors (FSHR). To assess the role of FSHR gene expression in regulating expression of FSHR protein in the plasma membrane, the effects of a porcine FSHR cDNA antise...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10310
更新日期:2003-06-01 00:00:00
abstract::Diandric and digynic triploid mouse embryos were isolated in the morning on day 10 of gestation. The embryos were separated from their extraembryonic membranes, and the latter were analysed cytogenetically by G-banding to establish the ploidy and sex chromosome constitution of these embryos. The diandric triploid embr...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080250409
更新日期:1990-04-01 00:00:00
abstract::We have tested three parameters in sperm-mediated gene transfer assays with mice and pigs: (i) the epididymal versus ejaculated origin of sperm cells, (ii) the primary structure, and (iii) the amount of the challenging foreign DNA. We have found that the pVLCNhGH construct, of retrotransposon origin, causes a massive ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200006)56:2+<301::AID-MRD2
更新日期:2000-06-01 00:00:00
abstract::Mouse half-morulae were cryopreserved less than or equal to 1, 3, 6, and 12 hr after bisection by the vitrification method using 25% glycerol and 25% 1,2-propanediol as cryoprotectant. The developmental rates of the frozen-thawed half-embryos to blastocysts in vitro were 77.8% (63/81), 82.0% (41/50), 92.1% (117/127), ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080300309
更新日期:1991-11-01 00:00:00
abstract::For mammalian organism, fertilization begins with species-specific recognition between sperm and egg, a process depending upon egg zona pellucida glycoproteins and putative sperm interacting protein(s). In mouse, zona pellucida glycoprotein ZP3 is believed to be the primary receptor for sperm and inducer of sperm acro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20705
更新日期:2007-10-01 00:00:00
abstract::The aim of the present series of experiments was to investigate the effect of the size of follicle from which the oocytes originate on their subsequent in vitro developmental ability. Ovarian follicles were isolated and grouped according to size (2-6 mm, > 6 mm). Primary oocytes were carefully liberated and grouped ac...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080370107
更新日期:1994-01-01 00:00:00
abstract::Post-acrosomal WW-domain binding protein (PAWP) is a signaling molecule located in the post-acrosomal sheath (PAS) of mammalian spermatozoa. We hypothesized that the proper integration of PAWP in the sperm PAS is reflective of bull-sperm quality and fertility. Cryopreserved semen samples from 298 sires of acceptable, ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22309
更新日期:2014-05-01 00:00:00
abstract::Connexin 43 (Cx43), a gap junction protein expressed in differentiated granulosa cells, is necessary for normal follicular development. Cx43 expression and regulation by epidermal growth factor (EGF) were characterized in immature rabbit granulosa cells. Cx43 mRNA was expressed in the granulosa cells of primary follic...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10219
更新日期:2003-01-01 00:00:00
abstract::Demecolcine-induced enucleation (IE) of mouse oocytes has been shown to improve development to term of cloned mice. In this study, we characterized the kinetics and morphological progression of bovine oocytes subjected to IE, and evaluated their ability to support embryo development to the blastocyst stage after nucle...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20356
更新日期:2005-10-01 00:00:00
abstract::Remodeling and relaxation of the mouse pubic symphysis (PS) are central events in parturition. The mouse PS remodels in a hormone-controlled process that involves the modification of the fibrocartilage into an interpubic ligament (IpL), followed by its relaxation prior to parturition. It is recognized that nitric oxid...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22020
更新日期:2012-04-01 00:00:00
abstract::Transcription factors inhibit or assist RNA polymerases in the initiation and maintenance of transcription; however, the cell specific expression and roles of transcription factors within bovine ovarian follicles during development are unknown. The aim of present study was to determine if the expression of transcripti...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20819
更新日期:2008-05-01 00:00:00
abstract::In this study cytoskeletal antigens common to brushtail possum and tammar wallaby spermatozoa were characterised using a monoclonal antibody (PSA-10). Using indirect immunofluorescence, the PSA-10 antibody detected antigens predominantly associated with the midpiece and principal piece of mature, permeabilised marsupi...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199808)50:4<461::AID-MRD10
更新日期:1998-08-01 00:00:00
abstract::The sequence of ionic changes involved in initiation of acrosomal exocytosis in capacitated mouse spermatozoa was investigated. Earlier studies demonstrated that a large influx of Na+ is required for exocytosis, this Na+ apparently being associated with an increase in intracellular pH (pHi) via an Na(+)-H+ exchanger. ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080360313
更新日期:1993-11-01 00:00:00
abstract::In the lizard Podarcis sicula, the major vitellogenin (VTG)-derived yolk proteins, lipovitellins and phosvitins, were extracted from the yolk globules of laid and fertilized eggs at different periods of incubation up to 44 days close to hatching. Embryonic development was almost over at this time. Yolk proteins were i...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.90018
更新日期:2002-11-01 00:00:00
abstract::Exposing day 5 bovine morulae to reactive oxygen species induces a delayed degeneration of some blastocysts on day 8 post-insemination (pi) but without affecting the blastocyst rates. The aim of this study was to characterize the resisting and the degenerating population of blastocysts. The kinetics of degeneration of...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20262
更新日期:2005-07-01 00:00:00
abstract::Lipopolysaccharide (LPS) induces germ cell apoptosis, but its mechanism of action is not clear. One possibility is that LPS regulates the expression of FAS ligand (FASLG) in Sertoli cells, which will then influence germ cell apoptosis. In this study, LPS reduced the viability of cultured, immature boar Sertoli cells i...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22534
更新日期:2015-11-01 00:00:00
abstract::This study was performed to investigate the effects of edaravone on hypoxia-induced trophoblast cell proliferation, apoptosis, and invasion. The trophoblast cell line HTR-8 (H8) was treated with cobalt chloride (CoCl2 ) with or without a 1-hr edaravone pretreatment, followed by assessment of intracellular reactive oxy...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22652
更新日期:2016-07-01 00:00:00
abstract::The present study has used methoxyacetic acid (MAA)-induced depletion of specific germ cell types in the rat and in situ hybridization with nonradioactive riboprobes to determine the stages of the spermatogenic cycle at which there is expression of the mRNA for the basic chromosomal protein transition protein 2 (TP2)....
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330404
更新日期:1992-12-01 00:00:00
abstract::Earlier work from this laboratory has determined that glucose plays an important role in the mechanisms regulating meiotic maturation in mammalian oocytes. In the current study, we have further explored the role of glucose in hormone-induced germinal vesicle breakdown (GVB) in an effort to better understand how glucos...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199605)44:1<121::AID-MRD14
更新日期:1996-05-01 00:00:00
abstract::Sequential culture and coculture are two methods of improving the development of preimplantation embryos in vitro. Direct comparison of the efficiency of these methods is limited. Proliferation and apoptosis determine the total number of blastomere in preimplantation embryo, which is a sensitive parameter for evaluati...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20048
更新日期:2004-05-01 00:00:00
abstract::The murine intracisternal A particle (IAP) proviral elements are expressed at low levels in undifferentiated F9 embryonal carcinoma cells but are highly expressed when F9 cells are induced to differentiate into parietal endoderm-like cells. IAP elements are also expressed in parietal endoderm-like PYS-2 cells. We prev...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080410103
更新日期:1995-05-01 00:00:00
abstract::Annexins are a family of Ca(2+)-binding proteins involved in the exocytotic process. The presence and the role of annexins in mammalian spermatozoa have not been well established. Two annexin-like proteins were obtained from guinea pig testis, a doublet of Mr 31-33 kD (p31/33) and a protein of Mr 50 kD (p50). Both pro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199603)43:3<366::AID-MRD11
更新日期:1996-03-01 00:00:00
abstract::Collagen is denatured in the gradual cervical ripening process during late pregnancy, already before the onset of final cervical ripening at parturition. Matrix Metallo Proteinases (MMPs) might be responsible for this process. To investigate the presence and potential function of MMPs at the different stages of the ri...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20908
更新日期:2008-11-01 00:00:00
abstract::Ejaculated boar spermatozoa exhibit two types of hyperactivation: full and non-full. Full-type hyperactivation is characterized by the asymmetrical bending of the entire middle piece-principal piece and a twisting/figure-eight-like trajectory, and can be induced by simple incubation with CaCl2 after preincubation with...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22921
更新日期:2017-11-01 00:00:00
abstract::The gonadotropin releasing hormone (GnRH) has long been considered as a neuropeptide involved in the control of the reproductive cycle. However, the presence of GnRH and its receptors in various tissues, including ovary and testis, suggests a role as autocrine/paracrine factor. In the present study, we report the expr...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1000
更新日期:2001-05-01 00:00:00
abstract::Members of the Pumilio (Pum) family of RNA-binding proteins act as translational repressors and are required for germ cell development and asymmetric division. We identified the chicken Pum1 and Pum2 genes and analyzed their expression patterns in various tissues. Comparative sequence analysis of the Pum1 and Pum2 pro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20765
更新日期:2008-01-01 00:00:00
abstract::Fragmentation occurs during early developmental stages of electrically activated oocytes and nuclear transfer (NT) embryos. It might contribute to the low developmental rate of porcine NT embryos. The present study was conducted to investigate whether the addition of sugars such as sorbitol or sucrose suppresses fragm...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20258
更新日期:2005-06-01 00:00:00
abstract::The objective of the present study was to determine whether glucocorticoid (GC) and its receptor (GC-R) are expressed in the porcine corpus luteum (CL), and whether GC influences porcine luteal hormone production. The gene expressions of 11beta-hydroxysteroid dehydrogenase type 1 (11-HSD1), type 2 (11-HSD2), GC-R, and...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20833
更新日期:2008-05-01 00:00:00
abstract::Activins, the dimeric polypeptides of inhibin beta-subunits, exhibit paracrine effects on cell proliferation, differentiation, and various other cell functions. The complex biological response to activin appears to involve multiple receptors. In the present study, we examined the isoform mRNA expression of both activi...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080380103
更新日期:1994-05-01 00:00:00