Differential expression of genes for transcription factors in theca and granulosa cells following selection of a dominant follicle in cattle.

abstract：:The localization of fibrillarin and nucleolin in the nuclei of mouse two-cell, four-cell, and eight-cell embryos has been studied using immunofluorescent staining with specific antibodies. In all of these cleavage stages, both antigens were associated exclusively with the peripheral region of the nucleolus precursor b...

journal_title：Molecular reproduction and development

authors： Baran V,Veselá J,Rehák P,Koppel J,Fléchon JE

更新日期：1995-03-01 00:00:00

abstract：:Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...

journal_title：Molecular reproduction and development

authors： Shankar S,Mohapatra B,Verma S,Selvi R,Jagadish N,Suri A

更新日期：2004-10-01 00:00:00

abstract：:RNA interference (RNAi) is an effective tool for studying gene function in oocytes, but no studies have targeted somatic cells of primary cultured cumulus cell-oocyte complexes (COCs). This is probably due to difficulty in introducing RNAi-inducing molecules, such as a short-hairpin RNA (shRNA) gene, into COCs by comm...

journal_title：Molecular reproduction and development

authors： Sugiura K,Su YQ,Eppig JJ

更新日期：2009-06-01 00:00:00

abstract：:The homeobox-containing gene family plays a pivotal role in regulating, patterning, and axial morphogenesis in the developing embryo. But there is still very little known about the expression and function of these genes in mammalian oocytes and preimplantation stage embryos. In this study we have used degenerate prime...

journal_title：Molecular reproduction and development

authors： Ponsuksili S,Wimmers K,Adjaye J,Schellander K

更新日期：2001-11-01 00:00:00

abstract：:The present study aims at quantification of gonadotropin releasing hormone (GnRH) by radioimmunoassay, relative expression of its mRNA by real-time PCR accompanied by its cellular localization in the rat ovary by immunonohistochemistry (IHC) during different time points of pregnancy. To determine the involvement of en...

journal_title：Molecular reproduction and development

authors： Sengupta A,Chakrabarti N,Sridaran R

更新日期：2008-06-01 00:00:00

abstract：:Germinal vesicle (GV) breakdown in mammalian oocytes is regulated by the activation of maturation promoting factor (MPF). We investigated a specific cdc2 kinase inhibitor, roscovitine, to maintain pig oocytes in the GV stage. Cumulus-oocyte complexes (COCs) were aspirated from slaughterhouse ovaries and cultured for 4...

journal_title：Molecular reproduction and development

authors： Ju JC,Tsay C,Ruan CW

更新日期：2003-04-01 00:00:00

abstract：:Hoechst 33342 (H342), in combination with ultraviolet (UV) irradiation, is frequently used to aid or confirm the enucleation of porcine oocytes in somatic cell nuclear transfer programs. The exposure of oocytes to H342 and UV irradiation has a deleterious effect on the development of in vitro-fertilized porcine oocyte...

journal_title：Molecular reproduction and development

authors： Gil MA,Maside C,Cuello C,Parrilla I,Vazquez JM,Roca J,Martinez EA

更新日期：2012-09-01 00:00:00

abstract：:Germ cells occupy a unique position in animal reproduction, development, and evolution. In sexually reproducing animals, only they can produce gametes and contribute genetically to subsequent generations. Nonetheless, germ line specification during embryogenesis is conceptually the same as the specification of any som...

journal_title：Molecular reproduction and development

authors： Ewen-Campen B,Schwager EE,Extavour CG

更新日期：2010-01-01 00:00:00

abstract：:We previously demonstrated that the progesterone-(P) initiated human sperm acrosome reaction (AR) was dependent on the presence of extracellular Na+ (Na(-)0). Moreover, Na(-)0 depletion resulted in a decreased cytosolic pH (pHi), suggesting involvement of a Na(+)-dependent pHi regulatory mechanism during the P-initiat...

journal_title：Molecular reproduction and development

authors： Garcia MA,Meizel S

更新日期：1999-02-01 00:00:00

abstract：:Plac1 is a recently identified, X-linked gene whose expression is restricted primarily to cells of the trophoblast lineage. It localizes to a chromosomal locus previously implicated in placental growth. We therefore sought to determine if Plac1 is necessary for placental and embryonic development by examining a mutant...

journal_title：Molecular reproduction and development

authors： Jackman SM,Kong X,Fant ME

更新日期：2012-08-01 00:00:00

abstract：:Tcp-11 is a candidate for a distorter gene within the t-complex on mouse chromosome 17; although t-complex genes appear to affect sperm function, relatively little is known about mechanisms whereby these genes might play a specific physiological role. We present evidence that the protein TCP-11 is found on the surface...

journal_title：Molecular reproduction and development

authors： Fraser LR,Hosseini R,Hanyalogou A,Talmor A,Dudley RK

更新日期：1997-11-01 00:00:00

abstract：:In mammals, Sry, Sox9, and M33 act as regulators at a chromatin level and promote the maturation of embryonic gonads into testes. Recently, it was shown that transcriptional regulation by DNA methylation plays crucial roles in gene expression during the differentiation and development of various cell types. To determi...

journal_title：Molecular reproduction and development

authors： Mizukami T,Kanai Y,Fujisawa M,Kanai-Azuma M,Kurohmaru M,Hayashi Y

更新日期：2008-06-01 00:00:00

abstract：:The goals of this work were to create germ-cell-stage-specific cDNA libraries from mouse spermatogenic cells and to employ a novel two-step genetic screen to identify gene sequences present during the critical meiotic stage of spermatogenesis. Highly enriched germ-cell fractions were prepared from adult and juvenile m...

journal_title：Molecular reproduction and development

authors： Caldwell KA,Wiltshire T,Handel MA

更新日期：1996-04-01 00:00:00

abstract：:The vascular cell responses to the type 1, 2, and 3 isoforms of transforming growth factor-beta (TGF-beta 1, TGF-beta 2, TGF-beta 3) were studied using bovine aortic endothelial (BAECs) and smooth muscle cells (BASMC3) as well as rat epididymal fat pad microvascular endothelia (RFCs). Three distinct bioassays indicate...

journal_title：Molecular reproduction and development

authors： Madri JA,Bell L,Merwin JR

更新日期：1992-06-01 00:00:00

abstract：:Myricetin is a natural flavonoid, particularly enriched in red wines, whose occurrence is widespread among plants. Despite extensive research, the beneficial effects of Myricetin on human health are still controversial. Here, we tested the estrogen-like effect of the phytoestrogen Myricetin on human ejaculated sperm b...

journal_title：Molecular reproduction and development

authors： Aquila S,Santoro M,De Amicis F,Guido C,Bonofiglio D,Lanzino M,Cesario MG,Perrotta I,Sisci D,Morelli C

更新日期：2013-02-01 00:00:00

abstract：:Exposure to nickel has been shown to cause damage to the testis in several animal models. It is not known if the testis expresses protein(s) that can bind nickel. To test this, we used a nickel-binding assay to isolate testicular nickel-binding proteins. We identified glutamate-ammonia ligase (GLUL) as a prominent nic...

journal_title：Molecular reproduction and development

authors： Sun Y,Ou Y,Cheng M,Ruan Y,van der Hoorn FA

更新日期：2011-02-01 00:00:00

abstract：:Collagen is denatured in the gradual cervical ripening process during late pregnancy, already before the onset of final cervical ripening at parturition. Matrix Metallo Proteinases (MMPs) might be responsible for this process. To investigate the presence and potential function of MMPs at the different stages of the ri...

journal_title：Molecular reproduction and development

authors： van Engelen E,Breeveld-Dwarkasing VN,Taverne MA,Everts ME,van der Weijden GC,Rutten VP

更新日期：2008-11-01 00:00:00

abstract：:The mouse metallothionein (MT) gene family consists of four known members (MT-I through IV) clustered on chromosome 8. Studies reported herein examine the expression and regulation of the MT-III and MT-IV genes in specific cell types in the maternal reproductive tract, developing embryo, and fetus known to express the...

journal_title：Molecular reproduction and development

authors： Liang L,Fu K,Lee DK,Sobieski RJ,Dalton T,Andrews GK

更新日期：1996-01-01 00:00:00

abstract：:Apoptosis is a well-established cellular mechanism for selective cell deletion during development. However, little is known about the expression of an apoptotic pathway and its role in determining the relative sensitivity of the early, pre-gastrula, avian embryo to stress-induced cell death. We examined the sensitivit...

journal_title：Molecular reproduction and development

authors： Muscarella DE,Rachlinski MK,Bloom SE

更新日期：1998-10-01 00:00:00

abstract：:The purposes of this study were to establish and to explore the biological basis of the chronic stress-induced premature ovarian failure (POF) model and to explore the therapeutic effects of the traditional Chinese medicine Muniziqi. Sexually matured female Sprague-Dawley rats were fed with spinach and cilantro in col...

journal_title：Molecular reproduction and development

authors： Keremu A,Yaoliwasi A,Tuerhong M,Kadeer N,Heyi,Yiming A,Yilike X

更新日期：2019-02-01 00:00:00

abstract：:Ran, a Ras-related GTPase, is required for transporting proteins in and out of the nucleus during interphase and for regulating the assembly of microtubules. cDNA cloning shows that rat testis, like mouse testis, expresses both somatic and testis-specific forms of Ran-GTPase. The presence of a homologous testis-specif...

journal_title：Molecular reproduction and development

authors： Kierszenbaum AL,Gil M,Rivkin E,Tres LL

更新日期：2002-09-01 00:00:00

abstract：:The completion of spermiogenesis requires condensation of the haploid spermatid genome. This task is accomplished in a gradual and relentless manner by first erasing the nucleosomal organization of chromatin while the DNA is protected by transient nuclear proteins TP1 and TP2. Then, the more permanent protamines come ...

journal_title：Molecular reproduction and development

authors： Kierszenbaum AL

更新日期：2001-04-01 00:00:00

abstract：:Meiotic progression requires the translation of maternal mRNAs in a strict temporal order. In isolated animal oocytes, translation of maternal mRNAs containing a cytoplasmic polyadenylation element (CPE), such as cyclin B, is activated by in vitro stimulation of meiotic resumption which induces phosphorylation of CPEB...

journal_title：Molecular reproduction and development

authors： Ochi H,Aoto S,Tachibana K,Hara M,Chiba K

更新日期：2016-01-01 00:00:00

abstract：:Izumo, a sperm membrane protein, is essential for gamete fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N-terminal domain for which neither the functions nor homologous sequences are known. In the present work we identified three novel proteins showing an N-terminal domain with significant homology t...

journal_title：Molecular reproduction and development

authors： Ellerman DA,Pei J,Gupta S,Snell WJ,Myles D,Primakoff P

更新日期：2009-12-01 00:00:00

abstract：:We have previously reported metabolic cooperation between Sertoli and peritubular myoid cells in terms of synthesis of one of the main testicular extracellular matrix (ECM) constituents, glycosaminoglycans (GAG). This study concerns Sertoli cell ECM-peritubular myoid cell interactions in terms of GAG synthesis. We hav...

journal_title：Molecular reproduction and development

authors： Raychoudhury SS,Blackshaw AW,Irving MG

更新日期：1993-06-01 00:00:00

abstract：:Homozygous deletion of the Lis1 gene (Lis1(-/-)) in mouse resulted in early embryonic lethality immediately after embryo implantation by an undefined mechanism. We seek to define the nature of this demise. LIS1 (pafah1b1) is a 46 kDa protein with seven tryptophan-aspartate (WD) repeats. It docks with many proteins and...

journal_title：Molecular reproduction and development

authors： Cahana A,Jin XL,Reiner O,Wynshaw-Boris A,O'Neill C

更新日期：2003-10-01 00:00:00

abstract：:Enzyme activities were determined quantitatively in individual rat oocytes to study their energy metabolism during maturation. Low hexokinase activity and high activities of lactate dehydrogenase and enzymes in the phosphate pathway, i.e., glucose 6-P and 6-P gluconate dehydrogenases, were characteristic of immature o...

journal_title：Molecular reproduction and development

authors： Tsutsumi O,Satoh K,Taketani Y,Kato T

更新日期：1992-11-01 00:00:00

abstract：:A porcine interleukin-6 (pIL-6) cDNA has been cloned from pig spleen cDNA library to provide information that would allow us to study IL-6 mRNA expression during pregnancy of several domestic Artiodactyla. The cDNA is 1058 bp long and with a single open reading frame that encodes a 212 amino acid polypeptide with 28-r...

journal_title：Molecular reproduction and development

authors： Mathialagan N,Bixby JA,Roberts RM

更新日期：1992-08-01 00:00:00

abstract：:Fresh cytosols extracted from unfertilized amphibian eggs contain a cytostatic factor (CSF) which arrests the cell cycle at metaphase when microinjected into cleaving blastomeres. This CSF is sensitive to Ca2+, and is designated primary CSF (1 degree CSF). During storage of Ca(2+)-containing cytosols at 2 degrees C, s...

journal_title：Molecular reproduction and development

authors： Zhang J,Masui Y

更新日期：1997-07-01 00:00:00

abstract：:The effect of two glycoproteins (estrus-associated glycoprotein [EGP] and tissue inhibitor of metalloproteinase [TIMP-1]) secreted by bovine oviduct cells on in vitro bovine embryo development was assessed. A first set of experiments was conducted to determine whether the embryotrophic activity of the bovine oviduct-c...

journal_title：Molecular reproduction and development

authors： Vansteenbrugge A,Van Langendonckt A,Massip A,Dessy F

更新日期：1997-04-01 00:00:00