Abstract：
:Homozygous deletion of the Lis1 gene (Lis1(-/-)) in mouse resulted in early embryonic lethality immediately after embryo implantation by an undefined mechanism. We seek to define the nature of this demise. LIS1 (pafah1b1) is a 46 kDa protein with seven tryptophan-aspartate (WD) repeats. It docks with many proteins and has been implicated in microtubular function, cell division, intercellular transport, and nuclear and cellular motility. Combined Western and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analyses showed that LIS1 expression from the blastocyst stage required new transcription from the embryonic genome. Consequently, the death of post-implantation embryos may not reflect the first time during development that LIS1 was required, rather, it may reflect the first time following depletion of gametic stores that its actions were essential. Following culture of blastocysts in vitro for 96 hr the inner cell mass (ICM) of null embryos were significantly smaller than ICM of wild-type siblings. Normal blastocyst outgrowths after 96-hr culture had high levels of LIS1 expression in the outer cells of developing ICM and extensive expression in trophoblast cells. Lis1(-/-) embryos had significantly smaller trophoblast nuclei than wild-type embryos. The results show that LIS1 expression is required for the continued normal development of the ICM and optimal trophoblast giant cell formation.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Cahana A,Jin XL,Reiner O,Wynshaw-Boris A,O'Neill Cdoi
10.1002/mrd.10339keywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
134-42issue
2eissn
1040-452Xissn
1098-2795journal_volume
66pub_type
杂志文章abstract：:Aluminum hydroxide gel (Al-gel), which is used as an adjuvant, can absorb macromolecules. We investigated the applicability of Al-gel to the sustained release of follicle-stimulating hormone (FSH) as a simplified method of superovulation (SOV) in rabbits. The responsiveness of rabbits to SOV by a single injection of F...
journal_title：Molecular reproduction and development
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pub_type： 杂志文章
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更新日期：1996-01-01 00:00:00
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更新日期：2008-06-01 00:00:00
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1002/mrd.20540
更新日期：2006-12-01 00:00:00
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pub_type： 传,历史文章,杂志文章
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更新日期：2009-10-01 00:00:00
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更新日期：1998-12-01 00:00:00
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更新日期：2001-03-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
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更新日期：1996-04-01 00:00:00
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pub_type： 杂志文章
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
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更新日期：2003-01-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1999-11-01 00:00:00
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更新日期：2016-06-01 00:00:00
