Abstract:
:The goals of this work were to create germ-cell-stage-specific cDNA libraries from mouse spermatogenic cells and to employ a novel two-step genetic screen to identify gene sequences present during the critical meiotic stage of spermatogenesis. Highly enriched germ-cell fractions were prepared from adult and juvenile mouse testes, and purity of these fractions was extensively analyzed by light and electron microscopy. Standard techniques were used to prepare cDNA libraries from populations of mixed leptotene and zygotene (L/Z) spermatocytes, pachytene (P) spermatocytes, and round spermatids. These libraries were analyzed with respect to representation of sequences from ubiquitously expressed genes, and from genes expressed at specific germ-cell stages as well as from genes expressed in testicular somatic cells. For the first step of the screening procedure, testicular cDNA was prepared from mutant mice carrying the T(X;11)38H chromosomal translocation that causes spermatogenic arrest at early meiotic prophase. This mixed cDNA probe was used to screen the libraries from L/Z and P spermatocytes to detect sequences failed to hybridize. The clones identified were characterized for ability to hybridize to various germ-cell-specific cDNAs to verify that they represented sequences present in normal spermatogenic meiotic cells. These clones were then subjected to a second screening with another mutant probe; this time the cDNA probe was from testes of sterile mice bearing the T(X;16)16H chromosomal translocation that causes spermatogenic arrest at late meiotic prophase. This screen identified 27 clones that were not represented in testicular cDNA from T38-bearing mice or from T16-bearing mice. These clones may represent sequences essential for normal completion of the genetic events of meiosis during spermatogenesis. Likewise, the secondary screen identified 19 clones that were not represented in testicular cDNA from T38-bearing mice but were represented in testicular cDNA of T16-bearing mice. These clones are thus gene sequences present in spermatogenic cells during the time from early meiotic prophase to mid-to-late prophase. This strategy represents the first use of genetic aberrations in differential screening to identify genes expressed at specific times during mammalian spermatogenesis.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Caldwell KA,Wiltshire T,Handel MAdoi
10.1002/(SICI)1098-2795(199604)43:4<403::AID-MRD1>subject
Has Abstractpub_date
1996-04-01 00:00:00pages
403-13issue
4eissn
1040-452Xissn
1098-2795pii
10.1002/(SICI)1098-2795(199604)43:4<403::AID-MRD1>journal_volume
43pub_type
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journal_title:Molecular reproduction and development
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pub_type: 杂志文章
doi:10.1002/mrd.22060
更新日期:2012-08-01 00:00:00
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doi:10.1002/mrd.1080350307
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更新日期:1998-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.22803
更新日期:2017-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20613
更新日期:2007-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199602)43:2<217::AID-MRD11
更新日期:1996-02-01 00:00:00
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doi:10.1002/mrd.22614
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pub_type: 杂志文章
doi:10.1002/mrd.1080400408
更新日期:1995-04-01 00:00:00
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更新日期:1995-01-01 00:00:00
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更新日期:2002-07-01 00:00:00
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更新日期:2018-02-01 00:00:00
abstract::cAMP-dependent protein kinase A (PKA) plays important regulatory roles during mouse spermatogenesis. PKA-mediated signaling has been shown to regulate gene expression, chromatin condensation, capacitation, and motility during sperm development and behavior, although how PKA is regulated in spatiotemporal manners durin...
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