Abstract：
:Enzyme activities were determined quantitatively in individual rat oocytes to study their energy metabolism during maturation. Low hexokinase activity and high activities of lactate dehydrogenase and enzymes in the phosphate pathway, i.e., glucose 6-P and 6-P gluconate dehydrogenases, were characteristic of immature oocytes. Hexokinase may be a rate-limiting enzyme that enables oocytes to use glucose as an energy source. During maturation, the activities of hexokinase, phosphofructokinase, and malate dehydrogenase increased significantly, suggesting that the glycolytic pathway, as well as the tricarboxylic acid cycle, developed as the first meiotic division proceeded. In contrast, the activities of glucose 6-P and 6-P gluconate dehydrogenases decreased in maturing oocytes. The observation that the enzyme pattern in mature oocytes resembles more closely that in somatic cells appears to be significant, especially in light of previous studies showing this developmental trend in preimplantation embryos.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Tsutsumi O,Satoh K,Taketani Y,Kato Tdoi
10.1002/mrd.1080330315keywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
333-7issue
3eissn
1040-452Xissn
1098-2795journal_volume
33pub_type
杂志文章abstract：:Mouse oocyte maturation inhibitory factors, on the basis of inhibitory activity of spontaneous germinal vesicle breakdown (GVBD) of denuded mouse oocytes in culture, were extracted and partially purified by reversed-phase resin adsorption and Sephadex G-100 and G-50 column chromatographies from the urine of pregnant w...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080340116
更新日期：1993-01-01 00:00:00
abstract：:In this study we have examined the effect of insulin like growth factor I (IGF-I) and its interaction with gonadotropins in the presence or absence of granulosa cell coculture on in vitro oocyte maturation (IVM) and their subsequent embryonic development in buffalo. We also have examined the role of IGF-I alone or in ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199803)49:3<277::AID-MRD8>
更新日期：1998-03-01 00:00:00
abstract：:During early placental development, tumor suppressors and oncogenes work synergistically to regulate cell proliferation and differentiation in a restrained manner compared with the uncontrollable growth in cancer. One example of this partnership is the regulation of the oncofetal protein HMGA2 by BRCA1. BRCA1 forms a ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23255
更新日期：2019-11-01 00:00:00
abstract：:In vitro embryo production is an established method for both humans and animals, but is fraught with inferior development and health issues in offspring born after in vitro fertilization procedures. Analysis of epigenetic changes caused by exposure to in vitro conditions should shed light on potential sources of these...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22922
更新日期：2017-12-01 00:00:00
abstract：:Expression of ovine interferon-tau (oIFNtau), a factor essential for the process of maternal recognition of pregnancy in ruminant ungulates, is restricted to the trophoblast. However, the molecular mechanisms by which oIFNtau expression is restricted to the trophectoderm have not been fully elucidated. The objective o...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20457
更新日期：2006-05-01 00:00:00
abstract：:The normal proto-oncogene c-fms encodes the macrophage growth factor (M-CSF) receptor involved in growth, survival, and differentiation along the monocyte-macrophage lineage of hematopoietic cell development. A major portion of our research concerns unraveling the temporal, molecular, and structural features that dete...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/(SICI)1098-2795(199701)46:1<96::AID-MRD15>
更新日期：1997-01-01 00:00:00
abstract：:Tektins are microtubule-associated cytoskeletal proteins that are expressed primarily in the male germ cell-lineage in centrioles and basal bodies and within ciliary and flagellar doublet microtubules. They are proposed to be important for axonemal architecture and microtubule stability in the sperm tail and in other ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20025
更新日期：2004-03-01 00:00:00
abstract：:We hypothesized that the testis-specific isoform of angiotensin-converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti-tACE antibody; (ii) characterize expression of tACE in bovine tes...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22790
更新日期：2017-05-01 00:00:00
abstract：:The sequence of ionic changes involved in initiation of acrosomal exocytosis in capacitated mouse spermatozoa was investigated. Earlier studies demonstrated that a large influx of Na+ is required for exocytosis, this Na+ apparently being associated with an increase in intracellular pH (pHi) via an Na(+)-H+ exchanger. ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080360313
更新日期：1993-11-01 00:00:00
abstract：:Copulation produces different stimuli in the female reproductive tract in camelids, which lead to ovulation. Expression of β-nerve growth factor (β-NGF) and its specific receptor, tropomyosin receptor kinase A (TrKA), was studied comparing the oviductal microenvironment of mated and nonmated llamas. β-NGF and TrKA wer...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23426
更新日期：2020-11-01 00:00:00
abstract：:Configuration of germinal vesicle (GV) chromatin has been studied and found correlated with the developmental competence of oocytes in several mammalian species. A common feature in the configuration of GV chromatin in the species studied so far is that the diffuse chromatin (the so called "NSN" pattern) condenses int...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20251
更新日期：2005-06-01 00:00:00
abstract：:Inner cell masses (ICM) from in vitro produced day 8 or 9 bovine blastocysts were isolated by immunosurgery and cultured under different conditions in order to establish which of two feeder cell types and culture media were most efficient in supporting attachment and outgrowth of the bovine ICM cells. The efficiency o...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080400408
更新日期：1995-04-01 00:00:00
abstract：:The sperm nucleus has a unique chromatin structure where the DNA is highly condensed and associated with specific proteins, the protamines. It is a nondividing cell which is also transcriptionally inactive. After fusion with an oocyte, the sperm nucleus undergoes decondensation and, in the same time, starts replicatio...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(200003)55:3<307::AID-MRD9>
更新日期：2000-03-01 00:00:00
abstract：:Studies of ovine interferon-tau (oIFNtau) gene regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, have been carried out, but a definitive mechanism for its spatial-temporal transcription has not been elucidated. Recently, specific binding regions for transcription factors AP-1 and...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10293
更新日期：2003-05-01 00:00:00
abstract：:Klinefelter syndrome (KS) is the most common genetic cause of human infertility, but the mechanism(s) responsible for its phenotype remain largely unknown. KS is associated with alterations in body composition and with a higher risk of developing metabolic diseases. We therefore hypothesized that KS men seeking fertil...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22608
更新日期：2016-03-01 00:00:00
abstract：:Carp hyosophorin (HSP) is purified from oocytes. It is a highly glycosylated protein (10% protein and 90% carbohydrate) of high molecular weight (>100 kDa) and is localized in the cortical granules of oocytes. During cortical reaction carp HSP is exocytosed into the perivitelline space and is rapidly cleaved to the lo...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199903)52:3<288::AID-MRD6>
更新日期：1999-03-01 00:00:00
abstract：:Previous work provided evidence of Na+/H+ exchanger activity in the apical domain of mouse trophectodermal plasma membranes that provides a route for entry of extracellular Na+ (Manejwala et al., 1989). This activity was hypothesized to contribute to the trans-trophectodermal Na+ flux that is required for blastocoel e...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199806)50:2<146::AID-MRD4>
更新日期：1998-06-01 00:00:00
abstract：:During implantation in mice, tissue inhibitor of matrix metalloproteinases-3 is believed to play a key role in inhibiting matrix metalloproteinase activity associated with embryo invasion and tissue remodeling. The first objective of this study was to quantitatively compare the steady-state mRNA levels of tissue inhib...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1018
更新日期：2001-06-01 00:00:00
abstract：:A high efficient and simple transgenic technology on mice and rabbits to transfect spermatozoa with exogenous DNA/DMSO complex to obtain transgenic offspring, which is namely called DMSO-sperm mediated gene transfer (SMGT). Mouse sperm could be either directly transfected via injection into testis or cultured in vitro...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20401
更新日期：2006-05-01 00:00:00
abstract：:Earlier work from this laboratory has determined that glucose plays an important role in the mechanisms regulating meiotic maturation in mammalian oocytes. In the current study, we have further explored the role of glucose in hormone-induced germinal vesicle breakdown (GVB) in an effort to better understand how glucos...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199605)44:1<121::AID-MRD14
更新日期：1996-05-01 00:00:00
abstract：:To establish reliable criteria for the evaluation of nuclear donor embryos, we studied the effect of cell number and cell size of in vitro produced day 6 donor morulae on the rate of blastocyst formation following nuclear transfer to in vitro matured oocytes. In experiment 1, donor embryos were divided into three grou...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080420107
更新日期：1995-09-01 00:00:00
abstract：:In this study, we sought to determine the mechanism of early sex reversal in a teleost by applying 4 hr feminization treatments to XY (17α-ethynylestradiol 2000 μg L(-1) ) and YY (6500 μg L(-1) ) Nile tilapia embryos on the first day post-fertilization (dpf). We then searched for changes in the expression profiles of ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22436
更新日期：2014-12-01 00:00:00
abstract：:Temporal pattern of expression of Cu/Zn and Mn superoxide dismutases (SODs) was investigated in bovine oocytes and embryos produced in vitro in two different culture conditions and in vivo after superovulation. SODs were examined at a transcriptional level in single oocytes and embryos by reverse transcriptase-polymer...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/1098-2795(200101)58:1<45::AID-MRD7>3.0.CO;
更新日期：2001-01-01 00:00:00
abstract：:Normal epididymal function, such as protein expression and secretion, is primarily regulated by testicular androgens and temperature. However, the role of spermatozoa in this critical process has never been studied. In order to determine whether sperm itself could regulate epididymal function, we have developed a cell...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20751
更新日期：2008-03-01 00:00:00
abstract：:We have cloned and sequenced the bovine NALP9 cDNA, which contains a 2991 bp long open reading frame with 76% homology to its human counterpart. The putative 996 amino acids protein presents the domain organization typical of the Nacht, Leucine rich repeat and Pyrin domain containing (NALP) family that includes the ma...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20298
更新日期：2005-08-01 00:00:00
abstract：:In vitro growth of preantral follicles has the potential to produce considerable numbers of competent oocytes for use in medicine, agriculture, and even wildlife conservation. The critical regulatory role of growth factors and hormones in the development of preantral follicles has been established. This study investig...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22131
更新日期：2013-01-01 00:00:00
abstract：:Homozygous deletion of the Lis1 gene (Lis1(-/-)) in mouse resulted in early embryonic lethality immediately after embryo implantation by an undefined mechanism. We seek to define the nature of this demise. LIS1 (pafah1b1) is a 46 kDa protein with seven tryptophan-aspartate (WD) repeats. It docks with many proteins and...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10339
更新日期：2003-10-01 00:00:00
abstract：:The frameworks (key mechanisms) of the self/non-self-discrimination systems that are found in various organisms have not been actively selected for, but have evolved by genetic drift such that the genetic frequency of random, advantageous mutations has increased within the genomes of these species by natural selection...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22134
更新日期：2013-01-01 00:00:00
abstract：:The production of chimeras, by use of cell transplantation, has proved to be highly valuable in studies of development by providing insights into cell fate, differentiation, and developmental potential. So far, chimeric honeybees have been created by nuclear transfer technologies. We have developed protocols to produc...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20435
更新日期：2006-04-01 00:00:00
abstract：:A quantitative primer extension method was used to measure the mass of histone gene transcripts in mature oocytes of the amphibian Xenopus laevis. The procedure, using a large excess of gene-specific oligonucleotide primer and continuous incorporation of a radiolabeled deoxynucleoside triphosphate precursor, is more s...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080250105
更新日期：1990-01-01 00:00:00
