Abstract:
:A quantitative primer extension method was used to measure the mass of histone gene transcripts in mature oocytes of the amphibian Xenopus laevis. The procedure, using a large excess of gene-specific oligonucleotide primer and continuous incorporation of a radiolabeled deoxynucleoside triphosphate precursor, is more sensitive and quantitative than primer extension assays employing end-labeled primers. It was determined that there are stoichiometric amounts, approximately 2 X 10(8) copies, of mRNA for each of the five major histone gene classes in mature Xenopus oocytes. These observations are consistent with a model whereby transcription of these genes is coordinately regulated in a cell cycle-independent manner during amphibian oogenesis.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Brashears-Macatee S,Hinkley C,Perry Mdoi
10.1002/mrd.1080250105subject
Has Abstractpub_date
1990-01-01 00:00:00pages
22-7issue
1eissn
1040-452Xissn
1098-2795journal_volume
25pub_type
杂志文章abstract::There are five methyl binding domain (MBD) proteins characterized by a methyl CpG-binding domain. Four MBD proteins (MeCP2 and MBDs 1-3) are linked to transcriptional repression and one (MBD4), to DNA repair. During preimplantation development, the embryo undergoes global demethylation following fertilization and sele...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20712
更新日期:2008-01-01 00:00:00
abstract::FGF-4 appears to be the first fibroblast growth factor (FGF) expressed during embryogenesis, and its expression is critical for early mammalian development. FGF-4 is expressed in the embryonic cell lines, F9, D3, and NT2/D1; but its expression in these cells is repressed upon differentiation. Transcription of the FGF-...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199711)48:3<301::AID-MRD1>
更新日期:1997-11-01 00:00:00
abstract::Remodeling and relaxation of the mouse pubic symphysis (PS) are central events in parturition. The mouse PS remodels in a hormone-controlled process that involves the modification of the fibrocartilage into an interpubic ligament (IpL), followed by its relaxation prior to parturition. It is recognized that nitric oxid...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22020
更新日期:2012-04-01 00:00:00
abstract::Inner cell masses (ICM) from in vitro produced day 8 or 9 bovine blastocysts were isolated by immunosurgery and cultured under different conditions in order to establish which of two feeder cell types and culture media were most efficient in supporting attachment and outgrowth of the bovine ICM cells. The efficiency o...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080400408
更新日期:1995-04-01 00:00:00
abstract::A number of cytokines and growth factors are known to modulate proliferation and differentiation of human endometrium. In this study, the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and VEGF receptors, fms-like tyrosine kinase (Flt1) and kinase insert domain-containi...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20104
更新日期:2004-08-01 00:00:00
abstract::Epigenetics includes the study of external factors that can influence the expression of genes by altering the accessibility of DNA through methylation. To investigate the epigenetic influence of season, sperm head shape, and semen storage on placental and fetal tissues, pregnancies were generated in the summer or wint...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23125
更新日期:2019-04-01 00:00:00
abstract::Decades worth of research have consistently shown the adverse effects of elevated temperatures on reproductive parameters of livestock species. The objective of this study was to evaluate the developmental and apoptotic responses of porcine in vitro fertilized (IVF) and parthenogenetically activated (PA) embryos heat ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20620
更新日期:2007-05-01 00:00:00
abstract::Estrogen plays an important role in the human reproductive system and its action is mediated mainly by two specific receptors: α (ERα) and β (ERβ). There are polymorphic variants in both ER genes, and studies showed their association with reproductive outcomes. We aimed to determine the distribution of ERα and ERβ gen...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21365
更新日期:2011-09-01 00:00:00
abstract::The gonadotropin releasing hormone (GnRH) has long been considered as a neuropeptide involved in the control of the reproductive cycle. However, the presence of GnRH and its receptors in various tissues, including ovary and testis, suggests a role as autocrine/paracrine factor. In the present study, we report the expr...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1000
更新日期:2001-05-01 00:00:00
abstract::Klinefelter syndrome (KS) is the most common genetic cause of human infertility, but the mechanism(s) responsible for its phenotype remain largely unknown. KS is associated with alterations in body composition and with a higher risk of developing metabolic diseases. We therefore hypothesized that KS men seeking fertil...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22608
更新日期:2016-03-01 00:00:00
abstract::An organized microtubular cytoskeleton was discovered in the cytoplasm of Xenopus laevis oocytes. The microtubules were observed in 10- to 30-micron cryostat sections by indirect immunoperoxidase labeling using an antibody to tubulin. A gradual extraction of cells with a nonionic detergent was essential for good penet...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080300410
更新日期:1991-12-01 00:00:00
abstract::DNA methylation/demethylation of donor genomes in recipient ooplasm after nuclear transfer occurs in a species-specific way. In cloned rabbit and bovine embryos, repetitive sequences maintain the donor-type methylation status, but typical demethylation of repetitive sequences takes place in cloned porcine embryos. To ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20421
更新日期:2006-03-01 00:00:00
abstract::Sequential culture and coculture are two methods of improving the development of preimplantation embryos in vitro. Direct comparison of the efficiency of these methods is limited. Proliferation and apoptosis determine the total number of blastomere in preimplantation embryo, which is a sensitive parameter for evaluati...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20048
更新日期:2004-05-01 00:00:00
abstract::In studies of somatic cell nuclear transfer (SCNT), the ability of factors within the oocyte to epigenetically reprogram transferred nuclei is essential for embryonic development of the clone to proceed. However, irregular patterns of X-chromosome inactivation, abnormal expression of imprinted genes, and genomic DNA h...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21306
更新日期:2011-05-01 00:00:00
abstract::Mouse egg activation, which includes release from meiotic metaphase II arrest, results from fertilization-induced increase in intracellular calcium concentration ([Ca2+]i). However, during egg activation caused by exposure to the protein synthesis inhibitor, cycloheximide, [Ca2+]i did not change. Although eggs fertili...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080410218
更新日期:1995-06-01 00:00:00
abstract::To establish reliable criteria for the evaluation of nuclear donor embryos, we studied the effect of cell number and cell size of in vitro produced day 6 donor morulae on the rate of blastocyst formation following nuclear transfer to in vitro matured oocytes. In experiment 1, donor embryos were divided into three grou...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420107
更新日期:1995-09-01 00:00:00
abstract::Tektins are microtubule-associated cytoskeletal proteins that are expressed primarily in the male germ cell-lineage in centrioles and basal bodies and within ciliary and flagellar doublet microtubules. They are proposed to be important for axonemal architecture and microtubule stability in the sperm tail and in other ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20025
更新日期:2004-03-01 00:00:00
abstract::Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the u...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22771
更新日期:2017-03-01 00:00:00
abstract::The mRNAs of the nuclear encoded genes, ornithine decarboxylase (ODCase) and poly(ADP)ribose polymerase (PADPRP), and the mitochondrial encoded genes, cytochrome oxidase I and II (COI and COII) and ATPase 6, are differentially expressed during spermatogenesis (Alcivar et al., 1989: Biol Reprod 41:1133; 1989: Dev Biol ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199603)43:3<283::AID-MRD1>
更新日期:1996-03-01 00:00:00
abstract::The objective of the present study was to investigate the ultrastructural consequences of vitrification of bovine oocytes at the metaphase II (MII) stage by the so-called "Open Pulled Straw" method. Oocytes were matured in vitro for 22 hr and cryopreserved by vitrification. After warming and additional 2 hr of culture...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200005)56:1<80::AID-MRD10>
更新日期:2000-05-01 00:00:00
abstract::Glycoprotein hormone receptors contain large extracellular domains encoded by multiple exons that can be alternatively spliced. Using human ovarian surface epithelium, we cloned two new splice variants of the human follicle-stimulating hormone receptor (FSH-R) gene, hFSH-R2 and hFSH-R3. The hFSH-R2 splice variant diff...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22227
更新日期:2013-11-01 00:00:00
abstract::Apoptosis is a well-established cellular mechanism for selective cell deletion during development. However, little is known about the expression of an apoptotic pathway and its role in determining the relative sensitivity of the early, pre-gastrula, avian embryo to stress-induced cell death. We examined the sensitivit...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199810)51:2<130::AID-MRD2>
更新日期:1998-10-01 00:00:00
abstract::Limited understanding of the importance of known pluripotency factors in pig embryonic stem cells (ESC) impedes the establishment and validation of porcine ESC lines. This study evaluated the expression of known mouse ESC and human ESC (hESC) pluripotency markers in in vivo inner cell mass (ICM) and in vitro-cultured ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20780
更新日期:2008-03-01 00:00:00
abstract::Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20164
更新日期:2004-10-01 00:00:00
abstract::The aim of this study was to elucidate the relationship between protein tyrosine phosphorylation state and sperm characteristics in frozen-stored spermatozoa of Japanese Black bulls. The spermatozoa were washed with PBS containing polyvinyl alcohol and then incubated with cell-permeable cAMP analog cBiMPS to induce fl...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21233
更新日期:2010-10-01 00:00:00
abstract::The plasmid DNA, pAcZ, containing Escherichia coli beta-galactosidase (lacZ) gene under the control of chicken beta-actin gene promoter was injected in a linearized form into the germinal disc of fertilized chick ova at the single-cell stage. The manipulated embryos were cultured by the method of Naito et al. (1990) u...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080370207
更新日期:1994-02-01 00:00:00
abstract::MicroRNAs (miRNAs) are small noncoding RNAs that orchestrate numerous cellular processes both under normal physiological conditions as well as in diseases. This review summarizes the functional roles and transcriptional regulation of the highly evolutionarily conserved miRNA, microRNA-31 (miR-31). miR-31 is an importa...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.22678
更新日期:2016-08-01 00:00:00
abstract::Formation and maintenance of testis cords during embryogenesis are essential for establishing testicular structure and function in adults. At least five genes (Wt1, Dhh, Sox8/Sox9, and Dax1) appear to be required for the maintenance of testis cord integrity in mice. Here, we report that RFX1 is specifically expressed ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22660
更新日期:2016-07-01 00:00:00
abstract::Pannexins (Panxs) are channel-forming proteins that have homology to the invertebrate gap junction proteins, the innexins. These proteins form membrane channels implicated in ATP release. To evaluate the role of Panxs in the male reproductive tract, we investigated the distribution and regulation of Panx1 and 3 in the...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21280
更新日期:2011-02-01 00:00:00
abstract::In general, maternal age affects the quality of oocytes and embryos. The present study aimed to examine the features and age-associated gene expression profiles of bovine oocytes and embryos as well as to discover possible countermeasures against age-associated events. Comprehensive gene expression assays of germinal ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22187
更新日期:2013-07-01 00:00:00