Abstract:
:Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the underlying biological mechanisms of porcine pluripotency. LIF-derived piPSCs were more successful than their FGF-derived counterparts in the generation of in vitro chimeras and in teratoma formation. When LIF piPSCs chimeras were transferred into surrogate sows and allowed to develop, only their prescence within the embryonic membranes could be detected. Whole-transcriptome analysis of the piPSCs and porcine neonatal fibroblasts showed that they clustered together, but apart from the two pluripotent cell populations of early porcine embryos, indicating incomplete reprogramming. Indeed, bioinformatic analysis of the pluripotency-related gene network of the LIF- versus FGF-derived piPSCs revealed that ZFP42 (REX1) expression was absent in both piPSC-like cells, whereas it was expressed in the porcine inner cell mass at Day 7/8. A second striking difference was the expression of ATOH1 in piPSC-like cells, which was absent in the inner cell mass. Moreover, our gene expression analyses plus correlation analyses of known pluripotency genes identified unique relationships between pluripotency genes in the inner cell mass, which are to some extent, in the piPSC-like cells. This deficiency in downstream gene activation and divergent gene expression may be underlie the inability to derive germ line-transmitting piPSCs, and provides unique insight into which genes are necessary to achieve fully reprogrammed piPSCs. 84: 229-245, 2017. © 2016 Wiley Periodicals, Inc.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Secher JO,Ceylan A,Mazzoni G,Mashayekhi K,Li T,Muenthaisong S,Nielsen TT,Li D,Li S,Petkov S,Cirera S,Luo Y,Thombs L,Kadarmideen HN,Dinnyes A,Bolund L,Roelen BA,Schmidt M,Callesen H,Hyttel P,Freude KKdoi
10.1002/mrd.22771subject
Has Abstractpub_date
2017-03-01 00:00:00pages
229-245issue
3eissn
1040-452Xissn
1098-2795journal_volume
84pub_type
杂志文章abstract::Under the influence of gonadotropins or growth factors, a close cooperation develops between cumulus cells and the oocyte that is implicated in transmitting signals involved in maintaining or releasing the meiotic arrest in the oocyte. While cyclic adenosine 5'-monophosphate (cAMP) is a key molecule in maintaining the...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.21332
更新日期:2011-10-01 00:00:00
abstract::In this study, we cloned and characterized the follicle stimulating hormone receptor (Fshr) and luteinizing hormone receptor (Lhr) cDNAs of pejerrey Odontesthes bonariensis, a species with temperature-dependent sex determination (TSD), and analyzed their expression in relation to Fshb and Lhb subunits during gonadogen...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21179
更新日期:2010-06-01 00:00:00
abstract::XIST is an X-linked, non-coding gene responsible for the cis induction of X-chromosome inactivation (XCI). Knockout of the XIST allele on an active X chromosome abolishes erroneous XCI and enhances the in vivo development of cloned mouse embryos by more than 10-fold. This study aimed to investigate whether a similar m...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22808
更新日期:2017-06-01 00:00:00
abstract::Fertilization causes mature oocytes or eggs to increase their concentrations of intracellular calcium ions (Ca²⁺) in all animals that have been examined, and such Ca²⁺ elevations, in turn, provide key activating signals that are required for non-parthenogenetic development. Several lines of evidence indicate that the ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.22222
更新日期:2013-10-01 00:00:00
abstract::Communication between the oocyte and its somatic cells has been shown to be important in oocyte development. Here we examined how the oocyte may be involved in bovine cumulus cell expansion. Intact bovine cumulus oocyte complexes (COC) were obtained by puncturing antral follicles. From the intact COC, oocytectomised c...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420214
更新日期:1995-10-01 00:00:00
abstract::These experiments were designed to test the effects of an electrofusion and an electroporation pulse on bovine sperm-hamster egg development. In experiment 1, single motile sperm were injected into the perivitelline space of each egg. A 4,500 V/cm, 30 microseconds fusion pulse (FP) was applied while sperm-egg membrane...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080270212
更新日期:1990-10-01 00:00:00
abstract::Following Northern analysis, GGT mRNA was found predominantly within the caput epididymides and kidney. The size of mRNAs for kidney, caput, corpus, and ductus deferens were 2.2, 2.3, 2.2, and 2.3 kb, respectively, whereas cauda showed a doublet of 2.2 and 2.3 kb. GGT transpeptidation and hydrolytic activity within ep...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280107
更新日期:1991-01-01 00:00:00
abstract::The expression of both inhibin alpha- and inhibin/activin beta A-subunit mRNA was examined in equine uteroplacental tissues collected during pregnancy (days 90 to 300). Northern blot analysis revealed that 5 transcripts (7.0, 4.1, 3.4, 2.6, 1.5 kb) of beta A-subunit were present, and the most abundantly expressed tran...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199708)47:4<363::AID-MRD2>
更新日期:1997-08-01 00:00:00
abstract::Insulators are located at the boundaries of differentially regulated genes and delimit their interactions by establishing independent chromatin structures. Recently, an insulator sequence has been found in the 5'-flanking region of arylsulfatase (ARS) gene from sea urchin. To investigate functional conservation of thi...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200011)57:3<232::AID-MRD4>3.0.CO
更新日期:2000-11-01 00:00:00
abstract::Myeloid cell leukemia-1 (MCL1), an anti-apoptotic member of the BCL2 family, is expressed abundantly in the testis. Previous characterization revealed that MCL1 is expressed exclusively in the Leydig cells in the mouse testis, yet what it does in these cells remains unknown. We therefore analyzed testosterone biosynth...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22614
更新日期:2016-03-01 00:00:00
abstract::The aim of the present study was to investigate the implication of protein kinase C (PKC) in the mouse egg activation process. We used OAG (1-oleoyl-2-acetyl-sn-glycerol) as a PKC activator, calphostin C as a specific PKC inhibitor, and the calcium ionophore A23187 as a standard parthenogenetic agent. The exposure of ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199710)48:2<292::AID-MRD18
更新日期:1997-10-01 00:00:00
abstract::Teleost fish exhibit remarkably diverse and plastic patterns of sexual development. One of the most fascinating modes of plasticity is functional sex change, which is widespread in marine fish including species of commercial importance; however, the regulatory mechanisms remain elusive. In this review, we explore such...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.22691
更新日期:2017-02-01 00:00:00
abstract::Glycoprotein hormone receptors contain large extracellular domains encoded by multiple exons that can be alternatively spliced. Using human ovarian surface epithelium, we cloned two new splice variants of the human follicle-stimulating hormone receptor (FSH-R) gene, hFSH-R2 and hFSH-R3. The hFSH-R2 splice variant diff...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22227
更新日期:2013-11-01 00:00:00
abstract::The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22425
更新日期:2014-12-01 00:00:00
abstract::The influence of somatic cells on oocyte penetrability was studied during in vitro maturation. Four experiments were carried out. In the first, pig oocytes were fertilized in vitro immediately after collection (immature oocytes) or after being cultured for 44 hr with cumulus cells connected to the whole wall of the ex...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080290107
更新日期:1991-05-01 00:00:00
abstract::Telomeres are specialized natural ends of eukaryotic chromosomes that, contrary to the ends of broken chromosomes, are stable and do not fuse with the ends of other chromosomes. In addition, telomeres protect chromosomal ends from degradation, facilitate completion of chromosomal DNA replication, and contribute to chr...
journal_title:Molecular reproduction and development
pub_type: 评论,杂志文章
doi:10.1002/1098-2795(200009)57:1<2::AID-MRD2>3.0.CO;2
更新日期:2000-09-01 00:00:00
abstract::Pigs have a unique, non-invasive epitheliochorial placenta where maternal and fetal layers lay in apposition. Indentation of fetal capillaries into the trophoblasts and maternal capillaries into the uterine epithelium reduce the distance between the fetal and maternal blood, ensuring nutrient transfer for proper conce...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.22847
更新日期:2017-09-01 00:00:00
abstract::In Ciona intestinalis, sperm penetration through the egg vitelline coat is an essential event of fertilization. We investigated whether trypsin- and chymotrypsin-like enzymes are involved in this event. Inhibitors and peptide substrates for chymotrypsin-like enzymes blocked the overall process of fertilization in a co...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080260405
更新日期:1990-08-01 00:00:00
abstract::The aim of the present study was to investigate the mechanism of androgen-mediated, prolonged sperm-storage in the female reproductive tract of the bat, Scotophilus heathii. The bat treated in vivo with flutamide, an androgen antagonist, showed loss of spermatozoa at the storage site, utero-tubal junction. Immunocytoc...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21328
更新日期:2011-07-01 00:00:00
abstract::Primordial germ cells (PGCs), the precursors of sperm or ova, could be used to generate transgenic animals and interspecies germ-line chimeras, which would facilitate the recovery of endangered species by making their access and manipulation in vitro easier. During early embryogenesis in avian species, PGCs are transp...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22802
更新日期:2017-06-01 00:00:00
abstract::A novel gene Ggnbp1 was identified during yeast two-hybrid screening of gametogenetin protein 1 (GGN1)-interacting proteins. Ggnbp1 gene was found in mouse, rat, and human genomes but not in sequenced yeast, worms, fly, or fish genomes. Northern blotting analysis revealed that the gene was specifically expressed in th...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20214
更新日期:2005-03-01 00:00:00
abstract::The objective of the present study was to investigate the ultrastructural consequences of vitrification of bovine oocytes at the metaphase II (MII) stage by the so-called "Open Pulled Straw" method. Oocytes were matured in vitro for 22 hr and cryopreserved by vitrification. After warming and additional 2 hr of culture...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200005)56:1<80::AID-MRD10>
更新日期:2000-05-01 00:00:00
abstract::It has been proposed that the viability of early mammalian embryos is associated with a metabolism that is "quiet" rather than "active" (Leese HJ, 2002:BioEssays 24:845-849). The data on which this hypothesis was based were largely drawn from measurements on the depletion and appearance of amino acids from the culture...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.20604
更新日期:2007-10-01 00:00:00
abstract::Ran, a Ras-related GTPase, is required for transporting proteins in and out of the nucleus during interphase and for regulating the assembly of microtubules. cDNA cloning shows that rat testis, like mouse testis, expresses both somatic and testis-specific forms of Ran-GTPase. The presence of a homologous testis-specif...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10164
更新日期:2002-09-01 00:00:00
abstract::The aim of this study was to elucidate the relationship between protein tyrosine phosphorylation state and sperm characteristics in frozen-stored spermatozoa of Japanese Black bulls. The spermatozoa were washed with PBS containing polyvinyl alcohol and then incubated with cell-permeable cAMP analog cBiMPS to induce fl...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21233
更新日期:2010-10-01 00:00:00
abstract::The internally fertilizing primitive frog Ascaphus truei (family Ascaphidae) from the Pacific Northwest is the only frog with an intromittent organ. The more advanced neobatrachian frog Eleutherodactylus coqui (family Leptodactylidae) from Puerto Rico has secondarily acquired internal fertilization but mates by cloaca...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20207
更新日期:2005-03-01 00:00:00
abstract::Sry expression is essential for initiating male sex differentiation, and the expression occurs only during a restricted period in the developing gonad. It is thought that Sry is part of a pathway of genes that regulate sex determination. Although the interactions of several genes with Sry expression have been suggeste...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10278
更新日期:2003-04-01 00:00:00
abstract::Mitogen-activated protein kinase (MAPK) plays a crucial role in meiotic maturation of mouse oocytes. In order to understand the mechanism by which MAPK regulates meiotic maturation, we examined the effects of the MAPK pathway inhibitor U0126 on microtubule organization, gamma-tubulin and nuclear mitotic apparatus prot...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20695
更新日期:2007-07-01 00:00:00
abstract::Oxygen consumption of preimplantation and early postimplantation mouse embryos has been measured using a novel noninvasive ultramicrofluorescence technique, based on an oil-soluble, nontoxic quaternary benzoid compound pyrene, whose fluorescence is quenched in the presence of oxygen. Pyruvate and glucose consumption, ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199608)44:4<476::AID-MRD7>
更新日期:1996-08-01 00:00:00
abstract::In the present study we analyse the nature and the functional significance of the spherical and fibrillo-granular structures appearing in the oocyte nucleus of the lizard Podarcis sicula, following the disappearance of the typical nucleolus. By LM and TEM approaches, we demonstrate that the fibrillo-granuli, containin...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199812)51:4<413::AID-MRD8>
更新日期:1998-12-01 00:00:00