Abstract:
:The gonadotropin releasing hormone (GnRH) has long been considered as a neuropeptide involved in the control of the reproductive cycle. However, the presence of GnRH and its receptors in various tissues, including ovary and testis, suggests a role as autocrine/paracrine factor. In the present study, we report the expression of the sGnRH-1 and sGnRH-2 genes encoding salmon GnRH in rainbow trout testis throughout testicular development and spermatogenesis. We demonstrate that both sGnRH mRNA are expressed prior of sexual differentiation. In adult, northern blot analysis indicates that sGnRH-2 transcripts are expressed in the testis at higher levels than sGnRH-1 messengers. Moreover, we observed that the expression of sGnRH-2, and not sGnRH-1, messengers was stage-dependent. sGnRH-2 mRNA expression decreases at the onset and progressively rebounds at the end of spermatogenesis. In addition, we demonstrate that a complex stage-dependent and differential splicing of the sGnRH-2 messengers occurs throughout spermatogenesis. We isolated five transcripts corresponding to sGnRH-2 messengers. Two of them may encode a novel and shortened GnRH-associated peptide containing 18 residues instead of 46. Our data provide new insight in the putative role of GnRH and GAP peptides as autocrine/paracrine factors of spermatogenesis.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Uzbekova S,Ferrière F,Guiguen Y,Bailhache T,Breton B,Lareyre JJdoi
10.1002/mrd.1000keywords:
subject
Has Abstractpub_date
2001-05-01 00:00:00pages
1-10issue
1eissn
1040-452Xissn
1098-2795journal_volume
59pub_type
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journal_title:Molecular reproduction and development
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更新日期:1990-02-01 00:00:00
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更新日期:2011-02-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
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更新日期:2018-03-01 00:00:00