Abstract：
:An exogenous supply of hematopoietic cytokines is essential for maintaining murine embryonic stem (ES) cells in a proliferative yet undifferentiated state. Recently, it was demonstrated that hematopoietic cytokines utilize the gp130 signal transduction pathway to maintain this phenotype, although their involvement toward maintaining porcine ES cell pluripotency has not been established. Therefore, the objective of this study was to determine the effectiveness of several heterologous hematopoietic cytokines at maintaining the isolated porcine inner cell masses (pICM) in an undifferentiated state. pICMs (day 7) were isolated by immunosurgery and cultured 4 days in one of six treatments: control medium, human leukemia inhibitory factor (hLIF; 1,000 mu/ml), human interleukin-6 (hIL-6; 100 ng/ml), hIL-6 + hIL-6 soluble receptor (hIL6 + sR; 100 ng/ml + 2.5 micrograms/ml), human oncostatin M (hOSM; 100 ng/ml), or rat ciliary neurotrophic factor (rCNTF; 100 ng/ml). All cytokines were prepared in Dulbecco's Modified Eagle's Medium/Ham's F-10 (1:1)-based medium. Morphology of pICMs was evaluated on a scale of 1 (fully undifferentiated) to 5 (fully differentiated) at 24-h intervals. Differentiation was significantly lower on day 2 for rCNTF vs. hLIF cultured pICMs (2.07 +/- 0.15 vs. 2.70 +/- 0.16; P < 0.05). Furthermore, addition of rCNTF gave the lowest overall mean differentiation score (2.53 +/- 0.15). However, none of the cytokines significantly delayed differentiation over controls for the 4-day culture period (P > 0.05). Since these heterologous cytokines were unable to inhibit differentiation, it is unlikely they will be beneficial towards isolating porcine ES cell lines under current conditions. Future work with homologous cytokines and dose effects may prove more beneficial.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Moore K,Piedrahita JAdoi
10.1002/(SICI)1098-2795(199610)45:2<139::AID-MRD5>subject
Has Abstractpub_date
1996-10-01 00:00:00pages
139-44issue
2eissn
1040-452Xissn
1098-2795pii
10.1002/(SICI)1098-2795(199610)45:2<139::AID-MRD5>journal_volume
45pub_type
杂志文章abstract：:Mitochondria play an important role in the integration and transmission of cell death signals mediated by the Bcl-2 family proteins. Experiments were conducted to determine whether the anti-apoptotic peptides BH4 domain of Bcl-xL (TAT-BH4) and Bax inhibitor peptide (BIP) suppresses heat stress (HS) injury in oocytes b...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20986
更新日期：2009-07-01 00:00:00
abstract：:A number of cytokines and growth factors are known to modulate proliferation and differentiation of human endometrium. In this study, the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and VEGF receptors, fms-like tyrosine kinase (Flt1) and kinase insert domain-containi...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20104
更新日期：2004-08-01 00:00:00
abstract：:Oocyte meiosis is arrested at prophase I by factors secreted from surrounding somatic cells after oocytes acquire meiotic competence at an early antral stage, and meiosis resumes in preovulatory follicles as a result of the luteinizing hormone (LH) surge. Recently, signaling by C-type natriuretic peptide (CNP) through...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22114
更新日期：2012-11-01 00:00:00
abstract：:The developmental pattern of expression of the Strongylocentrotus purpuratus CyIIIb actin gene was determined by RNA blot hybridizations carried out with a gene-specific probe and total embryonic RNA isolated from various stages of development. The results indicate that the CyIIIb mRNA is not detected in the maternal ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080010309
更新日期：1989-01-01 00:00:00
abstract：:Various thiol compounds are known to improve cytoplasmic and/or nuclear maturation of oocytes in vitro. The present study examined the effects of two thiol compounds, cysteine (0.1, 0.5, and 1.0 mM) and cysteamine (50, 100, and 200 microM), on cytoplasmic and nuclear maturation of canine oocytes. Oocytes collected fro...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20674
更新日期：2007-09-01 00:00:00
abstract：:Varicocele is a prevalent pathology among infertile men. The mechanisms linking this condition to infertility, however, are poorly understood. Our previous work showed a relationship between sperm functional quality and the ability of spermatozoa to respond to capacitating conditions with increased membrane fluidity a...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20611
更新日期：2006-12-01 00:00:00
abstract：:We have isolated and characterized a cDNA clone corresponding to a new member of bep (butanol, extracted, proteins) Paracentrotus lividus multigene family coding for cell surface proteins. The cDNA, called bep3, encodes a 370 amino acid protein and shares the same structural organization in the coding region with othe...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199605)44:1<36::AID-MRD4>3
更新日期：1996-05-01 00:00:00
abstract：:The present study was conducted to reveal effects of in ovo injection of nonsteroidal aromatase inhibitor (Fadrozole) or estradiol at day 3 of incubation on mRNA levels of P45017alphahydroxylase (P450c17), P450 aromatase (P450arom) and anti-Müllerian hormone (AMH) in the chicken gonads. The mRNA levels in the gonads a...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(200001)55:1<20::AID-MRD4>3
更新日期：2000-01-01 00:00:00
abstract：:The vascular cell responses to the type 1, 2, and 3 isoforms of transforming growth factor-beta (TGF-beta 1, TGF-beta 2, TGF-beta 3) were studied using bovine aortic endothelial (BAECs) and smooth muscle cells (BASMC3) as well as rat epididymal fat pad microvascular endothelia (RFCs). Three distinct bioassays indicate...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.1080320207
更新日期：1992-06-01 00:00:00
abstract：:Acrosomal reaction is an essential prerequisite to fertilization. The changes in lipid composition of sperm membranes cause fusion of the plasma and outer acrosomal membranes that results in the exocytosis of acrosomal contents. We report that both bull and rabbit spermatozoa contain a phosphatidylcholine-specific pho...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080330308
更新日期：1992-11-01 00:00:00
abstract：:Lactate produced by glycolysis in Sertoli cells (SCs) is the main energy substrate for developing germ cells and plays a vital role in spermatogenesis. MicroRNAs (miRNAs) function as posttranscriptional regulators of gene expression in biological processes. We have previously shown that hyperthermia (43°C, 30 min) pro...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23265
更新日期：2019-11-01 00:00:00
abstract：:Rainbow trout (Oncorhynchus mykiss) oocytes were incubated for 3 hr in ovarian fluid alone (CC), or cortisol-enriched ovarian fluid [100 or 1,000 ng ml(-1) (CL and CH, respectively)], after which they were fertilized; the growth and development of the embryos reared from these oocytes was monitored until first feed, a...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21239
更新日期：2010-10-01 00:00:00
abstract：:We have studied the presence of four sperm glycosidases, alpha-mannosidase, alpha-L- fucosidase and two beta-hexosaminidase isoforms, in 11 species of the genus Drosophila spanning approximately an evolutionary 60 MY period, and in Scaptodrosophila lebanonensis, belonging to the ancestor genus Scaptodrosophila. These ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20932
更新日期：2009-01-01 00:00:00
abstract：:We hypothesized that the testis-specific isoform of angiotensin-converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti-tACE antibody; (ii) characterize expression of tACE in bovine tes...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22790
更新日期：2017-05-01 00:00:00
abstract：:Fibulin-1 is a secreted protein associated with elastic matrix fibres and basement membranes. It plays a role in stabilizing blood vessels and can also regulate cell motility and invasiveness. We studied the regulation of the fibulin-1 gene in the rat and human endometrium, an organ where cyclic tissue remodeling and ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20079
更新日期：2004-07-01 00:00:00
abstract：:In mouse oocytes, meiotic induction by pharmacological activation of PRKA (adenosine monophosphate-activated protein kinase; formerly known as AMPK) or by hormones depends on stimulation of fatty acid oxidation (FAO). PRKA stimulates FAO by phosphorylating and inactivating acetyl CoA carboxylase (ACAC; formerly ACC), ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22505
更新日期：2015-09-01 00:00:00
abstract：:The homeobox-containing gene family plays a pivotal role in regulating, patterning, and axial morphogenesis in the developing embryo. But there is still very little known about the expression and function of these genes in mammalian oocytes and preimplantation stage embryos. In this study we have used degenerate prime...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1091
更新日期：2001-11-01 00:00:00
abstract：:Meiotic progression requires the translation of maternal mRNAs in a strict temporal order. In isolated animal oocytes, translation of maternal mRNAs containing a cytoplasmic polyadenylation element (CPE), such as cyclin B, is activated by in vitro stimulation of meiotic resumption which induces phosphorylation of CPEB...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22599
更新日期：2016-01-01 00:00:00
abstract：:Expression of ovine interferon-tau (oIFNtau) genes, essential for the maternal recognition of pregnancy in ruminant ungulates, is restricted to the trophoblast and is not detected in any other cell types or tissues. Substantial secretion of oIFNtau starts on day 12-13 of pregnancy (day 0 = day of estrus), reaches the ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20002
更新日期：2004-04-01 00:00:00
abstract：:Activins, the dimeric polypeptides of inhibin beta-subunits, exhibit paracrine effects on cell proliferation, differentiation, and various other cell functions. The complex biological response to activin appears to involve multiple receptors. In the present study, we examined the isoform mRNA expression of both activi...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080380103
更新日期：1994-05-01 00:00:00
abstract：:Exposure to nickel has been shown to cause damage to the testis in several animal models. It is not known if the testis expresses protein(s) that can bind nickel. To test this, we used a nickel-binding assay to isolate testicular nickel-binding proteins. We identified glutamate-ammonia ligase (GLUL) as a prominent nic...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21275
更新日期：2011-02-01 00:00:00
abstract：:DNA methylation/demethylation of donor genomes in recipient ooplasm after nuclear transfer occurs in a species-specific way. In cloned rabbit and bovine embryos, repetitive sequences maintain the donor-type methylation status, but typical demethylation of repetitive sequences takes place in cloned porcine embryos. To ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20421
更新日期：2006-03-01 00:00:00
abstract：:In this review, we discuss the expression, regulation, downstream mechanisms, and function of stress-induced stress enzymes in mammalian oocytes, peri-implantation embryos, and the stem cells derived from those embryos. Recent reports suggest that stress enzymes mediate developmental functions during early mammalian d...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.20787
更新日期：2008-04-01 00:00:00
abstract：:In this report we show that the first event of activation in the human oocyte, the Fertilization current (FC), is a slow transient outward current of 300 pA, which induces a gradual hyperpolarization of the plasma membrane from -20mV to -60mV, 60-120 min after insemination, followed by a repolarization to -20mV. Activ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080380212
更新日期：1994-06-01 00:00:00
abstract：:Previous studies have shown that in adult male mice, expression of the meg1 gene is restricted to meiotic and early postmeiotic testicular germ cells. We have now analyzed the expression of meg1 during postnatal testicular development and the comparable meiotic stages in the female. The 0.75 kb transcript for meg1 beg...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080380104
更新日期：1994-05-01 00:00:00
abstract：:The present studies were performed to establish the effects of the size and number of artificial holes produced in the zona pellucida (ZP) on hatching and trophoblast outgrowth in vitro. Limited partial zona dissection (PZD) produced small, narrow incisions, and zona drilling with acidic Tyrode's (AT) across a larger ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080300110
更新日期：1991-09-01 00:00:00
abstract：:Factors affecting the efficiency of producing transgenic rats by intracytoplasmic sperm injection (ICSI)-mediated DNA transfer were investigated. Epididymal spermatozoa from Sprague-Dawley (SD) rats were sonicated and/or frozen-thawed for cutting the tail and membrane disruption. The sperm heads were exposed for 1 min...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20223
更新日期：2005-04-01 00:00:00
abstract：:Artificial activation is required for successful intracytoplasmic sperm injection (ICSI) to induce haploidy pronuclear formation with extraction of second polar body. The present study showed that an additional treatment with Phorbol 12-myristate 13-acetate (PMA) followed by Ca(2+) ionophore treatment improved the rat...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20182
更新日期：2005-01-01 00:00:00
abstract：:Demecolcine-induced enucleation (IE) of mouse oocytes has been shown to improve development to term of cloned mice. In this study, we characterized the kinetics and morphological progression of bovine oocytes subjected to IE, and evaluated their ability to support embryo development to the blastocyst stage after nucle...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20356
更新日期：2005-10-01 00:00:00
abstract：:The objective of this study was to evaluate the effect of progesterone (P4), estradiol (E2), and cortisol (CO) at intraoviductal concentrations on bovine embryo development and quality in vitro. After fertilization of in vitro matured oocytes, zygotes were cultured for 8 days in synthetic oviductal fluid, supplemented...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23144
更新日期：2019-06-01 00:00:00
