Abstract:
:The developmental pattern of expression of the Strongylocentrotus purpuratus CyIIIb actin gene was determined by RNA blot hybridizations carried out with a gene-specific probe and total embryonic RNA isolated from various stages of development. The results indicate that the CyIIIb mRNA is not detected in the maternal pool, and, although the gene is activated at the early stages (about 10 hr postfertilization), considerable amounts of mRNA do not accumulate until well into the pluteus stage 3 days later. These results suggest either a post-transcriptional regulatory mechanism that governs early embryonic expression of the CyIIIb actin or a late embryonic transcriptional enhancement of this gene. We present here the complete nucleotide sequence of the CyIIIb gene, which lies within the 10,361 base pairs of the sequenced region. The entire transcription unit is 7,455 nt long and shares structural similarities with the other cytoskeletal-type actin genes from this sea urchin. Sequence comparisons of CyIIIb to the CyIIIa actin gene, to which it is linked, reveals extensive homology even in the introns. The deduced amino acid sequence of the CyIIIb actin shows five amino acid substitutions compared with the CyIIIa actin and nine when compared with the CyI, the endodermal embryonic cytoskeletal-type actin. Five out of these nine amino acid differences occur within a small peptide (position 257 to 267). The 5' flanking sequence of the CyIIIb gene shows a remarkable homology (approximately 75-80%) with the CyIIIa upstream region up to the position -200 and a lack of any obvious similarity further upstream. This observation suggests that the two genes possibly share some common regulatory factors.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Flytzanis CN,Bogosian EA,Niemeyer CCdoi
10.1002/mrd.1080010309subject
Has Abstractpub_date
1989-01-01 00:00:00pages
208-18issue
3eissn
1040-452Xissn
1098-2795journal_volume
1pub_type
杂志文章abstract::To investigate the decrease of transcription and to determine the localization of RNA molecules during spermiogenesis in the fern Scolopendrium, we have used the enzyme-gold electron microscope method. During cell differentiation, the labeling decreases over the cytoplasm and increases over the nucleus. In the latter,...
journal_title:Molecular reproduction and development
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