LIN28B regulates androgen receptor in human trophoblast cells through Let-7c.

abstract：:The neomycin-resistant gene (neo(r)) is probably the most commonly used selectable marker gene in gene targeting and gene transfection research. In this study, the neo(r) gene construct was introduced into in vitro cultured goat foetal fibroblast cells (IV-5), and the cells were selected with 900 microg/ml G418. The G...

journal_title：Molecular reproduction and development

authors： Zou X,Wang Y,Cheng Y,Yang Y,Ju H,Tang H,Shen Y,Mu Z,Xu S,Du M

更新日期：2002-02-01 00:00:00

abstract：:In the mammalian testis the cytoplasmic beta and gamma actins are expressed in all stages of germ-cell differentiation, whereas gamma enteric actin is expressed in germ cells solely in postmeiotic stages. Northern blot analysis of mouse testicular RNAs reveals actin mRNAs of about 2.1, 1.5, and 1.4 kB. The 2.1-kB mRNA...

journal_title：Molecular reproduction and development

authors： Gu W,Kwon YK,Hecht NB

更新日期：1996-06-01 00:00:00

abstract：:In this report we show that the first event of activation in the human oocyte, the Fertilization current (FC), is a slow transient outward current of 300 pA, which induces a gradual hyperpolarization of the plasma membrane from -20mV to -60mV, 60-120 min after insemination, followed by a repolarization to -20mV. Activ...

journal_title：Molecular reproduction and development

authors： Gianaroli L,Tosti E,Magli C,Iaccarino M,Ferraretti AP,Dale B

更新日期：1994-06-01 00:00:00

abstract：:These experiments were designed to test the effects of an electrofusion and an electroporation pulse on bovine sperm-hamster egg development. In experiment 1, single motile sperm were injected into the perivitelline space of each egg. A 4,500 V/cm, 30 microseconds fusion pulse (FP) was applied while sperm-egg membrane...

journal_title：Molecular reproduction and development

authors： Rickords LF,White KL,Wiltbank JN

更新日期：1990-10-01 00:00:00

abstract：:We previously showed that, during mouse oocyte maturation, specific maternal mRNAs (actins) are deadenylated, while others (hypoxanthine phosphoribosyltransferase:HPRT) are adenylated. As in other systems, these changes can be correlated with changes in translational activities. Maturation-specific polyadenylation in ...

journal_title：Molecular reproduction and development

authors： Paynton BV,Bachvarova R

更新日期：1994-02-01 00:00:00

abstract：:The aim of this study is to examine the effect of bovine oocyte maturation, fertilization or culture in vivo or in vitro on the proportion of oocytes reaching the blastocyst stage, and on blastocyst quality as measured by survival following vitrification. In Experiment 1, 4 groups of oocytes were used: (1) immature oo...

journal_title：Molecular reproduction and development

authors： Rizos D,Ward F,Duffy P,Boland MP,Lonergan P

更新日期：2002-02-01 00:00:00

abstract：:Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related ...

journal_title：Molecular reproduction and development

authors： Ma Q,Wang H,Guo R,Wang H,Ge Y,Ma J,Xue S,Han D

更新日期：2006-09-01 00:00:00

abstract：:To investigate the decrease of transcription and to determine the localization of RNA molecules during spermiogenesis in the fern Scolopendrium, we have used the enzyme-gold electron microscope method. During cell differentiation, the labeling decreases over the cytoplasm and increases over the nucleus. In the latter,...

journal_title：Molecular reproduction and development

authors： Rejon E,Bajon C,Blaize A,Robert D

更新日期：1988-01-01 00:00:00

abstract：:We have investigated the origin of the sperm motility inhibitor (SPMI) from boar seminal plasma. SPMI was measured by its capacity to inhibit the motility of demembranated spermatozoa and by an enzyme-linked immunosorbent assay (ELISA). Among the various reproductive and now reproductive tissues and fluids tested, onl...

journal_title：Molecular reproduction and development

authors： Iwamoto T,Tanaka H,Osada T,Shinagawa T,Osamura Y,Gagnon C

更新日期：1993-12-01 00:00:00

abstract：:Germ cells require communication with associated somatic cells for normal gametogenesis, as exemplified by an oocyte that interacts with granulosa cells via paracrine factors as well as gap junctions located at sites of contact between these two cell types. The objective of the present study was to define the mechanis...

journal_title：Molecular reproduction and development

authors： McGinnis LK,Kinsey WH

更新日期：2015-02-01 00:00:00

abstract：:A group of low Mr (16 kDa-23 kDa) glycoproteins on ejaculated boar spermatozoa have been shown to have high affinity for homologous zona pellucida glycoproteins (ZPGPs). These ZPGP binding proteins are derived from seminal plasma as shown by their absence from epididymal spermatozoa and their presence in seminal plasm...

journal_title：Molecular reproduction and development

authors： Parry RV,Barker PJ,Jones R

更新日期：1992-09-01 00:00:00

abstract：:Morphogenesis of the Eledone cirrhosa sperm nucleus, as studied by electron microscopic techniques, is compared with that of Octopus vulgaris. Both species of cephalopods belong to the family Octopodidae. The results indicate that extensive nuclear helicoidization during E. cirrhosa spermiogenesis is brought about by ...

journal_title：Molecular reproduction and development

authors： Gimenez-Bonafé P,Ribes E,Zamora MJ,Kasinsky HE,Chiva M

更新日期：2002-07-01 00:00:00

abstract：:We compared the results of intracytoplasmic sperm injection (ICSI) that leads to full term development of hybrid (B6C3F1 and B6D2F1) and inbred (C57BL/6) mouse embryos. Although fertilization and pre-implantation development of C57BL/6 eggs were similar to those of F1 hybrid eggs, post-implantation development of the ...

journal_title：Molecular reproduction and development

authors： Kawase Y,Iwata T,Toyoda Y,Wakayama T,Yanagimachi R,Suzuki H

更新日期：2001-09-01 00:00:00

abstract：:Fresh cytosols extracted from unfertilized amphibian eggs contain a cytostatic factor (CSF) which arrests the cell cycle at metaphase when microinjected into cleaving blastomeres. This CSF is sensitive to Ca2+, and is designated primary CSF (1 degree CSF). During storage of Ca(2+)-containing cytosols at 2 degrees C, s...

journal_title：Molecular reproduction and development

authors： Zhang J,Masui Y

更新日期：1997-07-01 00:00:00

abstract：:Embryonic stem (ES) cell lines are routinely derived from in vivo produced blastocysts. We investigated the efficiency of ES cells derivation from in vitro produced blastocysts either in monoculture or sequential culture. Zygotes from hybrid F1 B6D2 mice were cultured in vitro to the blastocyst stage in Potassium (K(+...

journal_title：Molecular reproduction and development

authors： Liu J,Schoonjans L,Tielens S,Speleman F,Cornelissen M,De Sutter P,Dhont M,Van der Elst J

更新日期：2006-08-01 00:00:00

abstract：:Dickkopf-like 1 (DkkL1) is related to the Dickkopf gene family, a group of proteins that are characterized as secreted antagonists of Wingless (Wnt) signal transduction proteins. DkkL1 mRNA is found in preimplantation mouse embryos and in developing neural tissue, but in adults it is found primarily in the testes. In ...

journal_title：Molecular reproduction and development

authors： Kohn MJ,Kaneko KJ,DePamphilis ML

更新日期：2005-08-01 00:00:00

abstract：:While most cyclin-dependent kinases (CDKs) are involved in cell cycle control, CDK5 is mostly known for crucial functions in neurogenesis. However, we cloned sea urchin CDK5 from a two-cell stage cDNA library and found that the protein is present in eggs and embryos, up to the pluteus stage, but without associated kin...

journal_title：Molecular reproduction and development

authors： Lozano JC,Schatt P,Vergé V,Gobinet J,Villey V,Peaucellier G

更新日期：2010-05-01 00:00:00

abstract：:A novel gene Ggnbp1 was identified during yeast two-hybrid screening of gametogenetin protein 1 (GGN1)-interacting proteins. Ggnbp1 gene was found in mouse, rat, and human genomes but not in sequenced yeast, worms, fly, or fish genomes. Northern blotting analysis revealed that the gene was specifically expressed in th...

journal_title：Molecular reproduction and development

authors： Zhou Y,Zhao Q,Bishop CE,Huang P,Lu B

更新日期：2005-03-01 00:00:00

abstract：:Annexins are a family of Ca(2+)-binding proteins involved in the exocytotic process. The presence and the role of annexins in mammalian spermatozoa have not been well established. Two annexin-like proteins were obtained from guinea pig testis, a doublet of Mr 31-33 kD (p31/33) and a protein of Mr 50 kD (p50). Both pro...

journal_title：Molecular reproduction and development

authors： Othón Hernández E,Roa-Espitia AL,Cruz Trejo J,Mújica A

更新日期：1996-03-01 00:00:00

abstract：:In this study, a combination of RNAi and endoscopic transfer to the oviduct of synchronized heifers has been used to investigate the effect of suppression of Cx43 and E-cadherin on the development, mRNA and protein expression of bovine blastocysts cultured in vitro or in vivo. In vitro matured and fertilized bovine zy...

journal_title：Molecular reproduction and development

authors： Tesfaye D,Lonergan P,Hoelker M,Rings F,Nganvongpanit K,Havlicek V,Besenfelder U,Jennen D,Tholen E,Schellander K

更新日期：2007-08-01 00:00:00

abstract：:Topoisomerase II (topo II) is an essential enzyme that alters DNA topology. This activity is important for a variety of chromosome functions including DNA replication, transcription, recombination, and chromosome condensation and segregation. Previously we localized topo II in mouse gametes and preimplantation embryos...

journal_title：Molecular reproduction and development

authors： St Pierre J,Wright DJ,Rowe TC,Wright SJ

更新日期：2002-03-01 00:00:00

abstract：:The present study has used methoxyacetic acid (MAA)-induced depletion of specific germ cell types in the rat and in situ hybridization with nonradioactive riboprobes to determine the stages of the spermatogenic cycle at which there is expression of the mRNA for the basic chromosomal protein transition protein 2 (TP2)....

journal_title：Molecular reproduction and development

authors： Saunders PT,Millar MR,Maguire SM,Sharpe RM

更新日期：1992-12-01 00:00:00

abstract：:The aims of this study were to investigate steady-state level of Kit Ligand (KL) mRNA and its effects on in vitro survival and growth of caprine preantral follicles. RT-PCR was used to analyze caprine steady-state level of KL mRNA in primordial, primary, and secondary follicles, and in small (1-3 mm) and large (3-6 mm...

journal_title：Molecular reproduction and development

authors： Celestino JJ,Bruno JB,Lima-Verde IB,Matos MH,Saraiva MV,Chaves RN,Martins FS,Almeida AP,Cunha RM,Lima LF,Name KP,Campello CC,Silva JR,Báo SN,Figueiredo JR

更新日期：2010-03-01 00:00:00

abstract：:We have studied the presence of four sperm glycosidases, alpha-mannosidase, alpha-L- fucosidase and two beta-hexosaminidase isoforms, in 11 species of the genus Drosophila spanning approximately an evolutionary 60 MY period, and in Scaptodrosophila lebanonensis, belonging to the ancestor genus Scaptodrosophila. These ...

journal_title：Molecular reproduction and development

authors： Intra J,Cenni F,Pavesi G,Pasini M,Perotti ME

更新日期：2009-01-01 00:00:00

abstract：:It is known that the hypothalamic-pituitary-gonadal axis participates in the sex change of hermaphrodite teleosts, and gonadal steroid hormones mediate this physiological process. The secretion of gonadal steroids is directly regulated by signaling pathways involving gonadotropins (GtHs) and gonadotropin receptors (Gt...

journal_title：Molecular reproduction and development

authors： Hu X,Liu X,Zhang H,Zhang Y,Li S,Sang Q,Wang Q,Luo W,Liu Q,Lu D,Meng Z,Lin H

更新日期：2011-06-01 00:00:00

abstract：:We have developed a novel reverse transcription-polymerase chain reaction (RT-PCR)-based approach for systematically quantifying in a single experiment the abundances of many different mRNAs in preimplantation mouse embryos. With this approach, the entire mRNA population from a small number of embryos is amplified whi...

journal_title：Molecular reproduction and development

authors： Rambhatla L,Patel B,Dhanasekaran N,Latham KE

更新日期：1995-07-01 00:00:00

abstract：:We hypothesized that the testis-specific isoform of angiotensin-converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti-tACE antibody; (ii) characterize expression of tACE in bovine tes...

journal_title：Molecular reproduction and development

authors： Ojaghi M,Kastelic J,Thundathil J

更新日期：2017-05-01 00:00:00

abstract：:Exposing day 5 bovine morulae to reactive oxygen species induces a delayed degeneration of some blastocysts on day 8 post-insemination (pi) but without affecting the blastocyst rates. The aim of this study was to characterize the resisting and the degenerating population of blastocysts. The kinetics of degeneration of...

journal_title：Molecular reproduction and development

authors： Feugang JM,Donnay I,Mermillod P,Marchandise J,Lequarre AS

更新日期：2005-07-01 00:00:00

abstract：:Copulation produces different stimuli in the female reproductive tract in camelids, which lead to ovulation. Expression of β-nerve growth factor (β-NGF) and its specific receptor, tropomyosin receptor kinase A (TrKA), was studied comparing the oviductal microenvironment of mated and nonmated llamas. β-NGF and TrKA wer...

journal_title：Molecular reproduction and development

authors： Sari LM,Zampini R,Argañaraz ME,Ratto MH,Apichela SA

更新日期：2020-11-01 00:00:00

abstract：:The mRNA translational control protein, Musashi, plays a critical role in cell fate determination through sequence-specific interactions with select target mRNAs. In proliferating stem cells, Musashi exerts repression of target mRNAs to promote cell cycle progression. During stem cell differentiation, Musashi target m...

journal_title：Molecular reproduction and development

authors： Arumugam K,Macnicol MC,Macnicol AM

更新日期：2012-08-01 00:00:00