Abstract:
:Embryonic stem (ES) cell lines are routinely derived from in vivo produced blastocysts. We investigated the efficiency of ES cells derivation from in vitro produced blastocysts either in monoculture or sequential culture. Zygotes from hybrid F1 B6D2 mice were cultured in vitro to the blastocyst stage in Potassium (K(+)) simplex optimised medium (KSOM) throughout or in KSOM and switched to COOK blastocyst medium on day 3 (KSOM-CBM). Blastocysts were explanted on a feeder layer of mitomycin C-inactivated murine embryonic fibroblasts (MEF) in TX-WES medium for ES cell derivation. Sequential KSOM-CBM resulted in improved blastocyst formation compared to KSOM monoculture. ES cells were obtained from 32.1% of explanted blastocsyts cultured in KSOM-CBM versus 18.4% in KSOM alone. ES cell lines were characterized by morphology, expression of SSEA-1, Oct-4 and alkaline phosphatase activity, and normal karyotype. These results indicate that in vitro culture systems to produce blastocysts can influence the efficiency of ES cell line derivation.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Liu J,Schoonjans L,Tielens S,Speleman F,Cornelissen M,De Sutter P,Dhont M,Van der Elst Jdoi
10.1002/mrd.20512subject
Has Abstractpub_date
2006-08-01 00:00:00pages
1017-21issue
8eissn
1040-452Xissn
1098-2795journal_volume
73pub_type
杂志文章abstract::We have developed a novel reverse transcription-polymerase chain reaction (RT-PCR)-based approach for systematically quantifying in a single experiment the abundances of many different mRNAs in preimplantation mouse embryos. With this approach, the entire mRNA population from a small number of embryos is amplified whi...
journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080290107
更新日期:1991-05-01 00:00:00
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更新日期:2014-07-01 00:00:00
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更新日期:1996-07-01 00:00:00
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