Effect of microinjection and two types of electrical stimuli on bovine sperm-hamster egg penetration.

abstract：:The goals of this work were to create germ-cell-stage-specific cDNA libraries from mouse spermatogenic cells and to employ a novel two-step genetic screen to identify gene sequences present during the critical meiotic stage of spermatogenesis. Highly enriched germ-cell fractions were prepared from adult and juvenile m...

journal_title：Molecular reproduction and development

authors： Caldwell KA,Wiltshire T,Handel MA

更新日期：1996-04-01 00:00:00

abstract：:Rainbow trout (Oncorhynchus mykiss) oocytes were incubated for 3 hr in ovarian fluid alone (CC), or cortisol-enriched ovarian fluid [100 or 1,000 ng ml(-1) (CL and CH, respectively)], after which they were fertilized; the growth and development of the embryos reared from these oocytes was monitored until first feed, a...

journal_title：Molecular reproduction and development

authors： Li M,Bureau DP,King WA,Leatherland JF

更新日期：2010-10-01 00:00:00

abstract：:Inhibin α (Inha) gene expression is regulated, in rat granulosa cells, via a cyclic 3',5'-adenosine monophosphate (AMP)-response element (CRE) found in a region of the promoter that is homologous to the human INHA promoter. We previously found that during in vitro cytotrophoblast differentiation, human INHA gene expre...

journal_title：Molecular reproduction and development

authors： Depoix CL,Debiève F,Hubinont C

更新日期：2014-11-01 00:00:00

abstract：:Cattle consuming endophyte-infected tall fescue grass have an associated reduction in circulating progesterone and reduced reproductive rates. In this study, commercially available rat microarrays were used to analyze the gene expression in luteal tissues from heifers fed endophyte-free fescue, endophyte-infected fesc...

journal_title：Molecular reproduction and development

authors： Jones KL,King SS,Iqbal MJ

更新日期：2004-02-01 00:00:00

abstract：:The aim of this study is to examine the effect of bovine oocyte maturation, fertilization or culture in vivo or in vitro on the proportion of oocytes reaching the blastocyst stage, and on blastocyst quality as measured by survival following vitrification. In Experiment 1, 4 groups of oocytes were used: (1) immature oo...

journal_title：Molecular reproduction and development

authors： Rizos D,Ward F,Duffy P,Boland MP,Lonergan P

更新日期：2002-02-01 00:00:00

abstract：:The present study examined the effect of elevated Ca(2+) concentration in fusion/activation medium on the fusion and development of fetal fibroblast nuclear transfer (NT) porcine embryos. Frozen-thawed and serum starved fetal fibroblasts were transferred into the perivitelline space of enucleated oocytes. Cell fusion ...

journal_title：Molecular reproduction and development

authors： Cheong HT,Park KW,Im GS,Lai L,Sun QY,Day BN,Prather RS

更新日期：2002-04-01 00:00:00

abstract：:Transcription factors inhibit or assist RNA polymerases in the initiation and maintenance of transcription; however, the cell specific expression and roles of transcription factors within bovine ovarian follicles during development are unknown. The aim of present study was to determine if the expression of transcripti...

journal_title：Molecular reproduction and development

authors： Zielak AE,Canty MJ,Forde N,Coussens PM,Smith GW,Lonergan P,Ireland JJ,Evans AC

更新日期：2008-05-01 00:00:00

abstract：:XIST is an X-linked, non-coding gene responsible for the cis induction of X-chromosome inactivation (XCI). Knockout of the XIST allele on an active X chromosome abolishes erroneous XCI and enhances the in vivo development of cloned mouse embryos by more than 10-fold. This study aimed to investigate whether a similar m...

journal_title：Molecular reproduction and development

authors： Yang Y,Wu D,Liu D,Shi J,Zhou R,He X,Quan J,Cai G,Zheng E,Wu Z,Li Z

更新日期：2017-06-01 00:00:00

abstract：:Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...

journal_title：Molecular reproduction and development

authors： Shankar S,Mohapatra B,Verma S,Selvi R,Jagadish N,Suri A

更新日期：2004-10-01 00:00:00

abstract：:Mitochondria play an important role in the integration and transmission of cell death signals mediated by the Bcl-2 family proteins. Experiments were conducted to determine whether the anti-apoptotic peptides BH4 domain of Bcl-xL (TAT-BH4) and Bax inhibitor peptide (BIP) suppresses heat stress (HS) injury in oocytes b...

journal_title：Molecular reproduction and development

authors： Soto P,Smith LC

更新日期：2009-07-01 00:00:00

abstract：:The production of chimeras, by use of cell transplantation, has proved to be highly valuable in studies of development by providing insights into cell fate, differentiation, and developmental potential. So far, chimeric honeybees have been created by nuclear transfer technologies. We have developed protocols to produc...

journal_title：Molecular reproduction and development

authors： Bergem M,Norberg K,Roseth A,Meuwissen T,Lien S,Aamodt RH

更新日期：2006-04-01 00:00:00

abstract：:Activation of bovine oocytes by experimental procedures that closely mimic normal fertilization and allow to obtain haploid oocytes is essential both for intracytoplasmic sperm injection (ICSI) and for nuclear transfer. Therefore, with the goal of producing haploid activated oocytes, this study evaluated whether bohem...

journal_title：Molecular reproduction and development

authors： Alberio R,Kubelka M,Zakhartchenko V,Hajdúch M,Wolf E,Motlik J

更新日期：2000-04-01 00:00:00

abstract：:Activins, the dimeric polypeptides of inhibin beta-subunits, exhibit paracrine effects on cell proliferation, differentiation, and various other cell functions. The complex biological response to activin appears to involve multiple receptors. In the present study, we examined the isoform mRNA expression of both activi...

journal_title：Molecular reproduction and development

authors： Wu TC,Jih MH,Wang L,Wan YJ

更新日期：1994-05-01 00:00:00

abstract：:In vitro culture of mammalian preimplantation embryos is associated with various developmental disorders such as retardation in development and cell damage. The molecular mechanisms underlying these processes are poorly understood. One of the possible reasons may be the unphysiologically high oxygen concentration used...

journal_title：Molecular reproduction and development

authors： Koerber S,Santos AN,Tetens F,Küchenhoff A,Fischer B

更新日期：1998-04-01 00:00:00

abstract：:In mammals, the nucleolus of full-grown oocyte is essential for embryonic development but not for oocyte maturation. In our study, the role of the growing oocyte nucleolus in oocyte maturation was examined by nucleolus removal and/or transfer into previously enucleolated, growing (around 100 µm in diameter) or full-gr...

journal_title：Molecular reproduction and development

authors： Kyogoku H,Ogushi S,Miyano T,Fulka J Jr

更新日期：2011-06-01 00:00:00

abstract：:Klinefelter syndrome (KS) is the most common genetic cause of human infertility, but the mechanism(s) responsible for its phenotype remain largely unknown. KS is associated with alterations in body composition and with a higher risk of developing metabolic diseases. We therefore hypothesized that KS men seeking fertil...

journal_title：Molecular reproduction and development

authors： Alves MG,Martins AD,Jarak I,Barros A,Silva J,Sousa M,Oliveira PF

更新日期：2016-03-01 00:00:00

abstract：:The presence and possible role of products of nuclear (c-fos and c-jun) and c-ras proto-oncogenes were investigated in preimplantation embryonic development in mice. Polyclonal antibodies to c-fos or c-jun proto-oncogene products did not affect development of in vitro-cultured embryos from two-cell to morula or from m...

journal_title：Molecular reproduction and development

authors： Ahmad K,Naz RK

更新日期：1993-11-01 00:00:00

abstract：:The effects of cryopreservation on the frequency and type of chromosomal abnormalities in human sperm were investigated. Employing a technique that enables direct visualization of human sperm chromosomes following in vitro penetration of hamster oocytes, sperm samples from 10 normal men were examined before and after ...

journal_title：Molecular reproduction and development

authors： Martin RH,Chernos JE,Rademaker AW

更新日期：1991-10-01 00:00:00

abstract：:In the equine, the zona pellucida (ZP) is the major barrier to successful in vitro fertilization. Therefore the aim of our studies was to analyze species-specific features of the equine ZP in regard to structure and glycoprotein ZPB and ZPC expression sites during oocyte development and embryogenesis. The equine ZP re...

journal_title：Molecular reproduction and development

authors： Kölle S,Dubois CS,Caillaud M,Lahuec C,Sinowatz F,Goudet G

更新日期：2007-07-01 00:00:00

abstract：:Insulators are located at the boundaries of differentially regulated genes and delimit their interactions by establishing independent chromatin structures. Recently, an insulator sequence has been found in the 5'-flanking region of arylsulfatase (ARS) gene from sea urchin. To investigate functional conservation of thi...

journal_title：Molecular reproduction and development

authors： Takada T,Iida K,Akasaka K,Yasue H,Torii R,Tsujimoto G,Taira M,Kimura H

更新日期：2000-11-01 00:00:00

abstract：:One of the hallmarks of mammalian sperm capacitation is the loss of cholesterol from the plasma membrane. Cholesterol has been associated with the formation of detergent insoluble membrane microdomains in many cell types, and sperm from several mammalian species have been shown to contain detergent-resistant membranes...

journal_title：Molecular reproduction and development

authors： Thaler CD,Thomas M,Ramalie JR

更新日期：2006-12-01 00:00:00

abstract：:The aim of this study was to examine the expression and regulation of peroxisome proliferator-activated receptor (PPAR) PPARdelta gene in mouse uterus during early pregnancy by in situ hybridization and immunohistochemistry. PPARdelta expression under pseudopregnancy, delayed implantation, hormonal treatment, and arti...

journal_title：Molecular reproduction and development

authors： Ding NZ,Teng CB,Ma H,Ni H,Ma XH,Xu LB,Yang ZM

更新日期：2003-11-01 00:00:00

abstract：:Proteins synthesized by DDK mice embryos were analyzed by 2D electrophoresis and a new egg-specific polypeptide, D14, was identified. The protein is characterized by its high rate of synthesis and electrophoretic properties (MW 36,500, pl greater than 8). The synthesis of D14 is strictly developmentally regulated: sta...

journal_title：Molecular reproduction and development

authors： Richoux V,Renard JP,Babinet C

更新日期：1991-03-01 00:00:00

abstract：:In mouse oocytes, meiotic induction by pharmacological activation of PRKA (adenosine monophosphate-activated protein kinase; formerly known as AMPK) or by hormones depends on stimulation of fatty acid oxidation (FAO). PRKA stimulates FAO by phosphorylating and inactivating acetyl CoA carboxylase (ACAC; formerly ACC), ...

journal_title：Molecular reproduction and development

authors： Valsangkar DS,Downs SM

更新日期：2015-09-01 00:00:00

abstract：:Triosephosphate isomerase 1 (TPI1) is a member of the glycolytic pathway, which is a critical source of energy for motility in mouse sperm. By immunoblotting, we detected two male, germ line-specific TPI1 bands (Mr 33,400 and 30,800) as well as the somatic-type band (Mr 27,700). Although all three bands were observed ...

journal_title：Molecular reproduction and development

authors： Ijiri TW,Vadnais ML,Lin AM,Huang AP,Cao W,Merdiushev T,Gerton GL

更新日期：2013-10-01 00:00:00

abstract：:Mouse half-morulae were cryopreserved less than or equal to 1, 3, 6, and 12 hr after bisection by the vitrification method using 25% glycerol and 25% 1,2-propanediol as cryoprotectant. The developmental rates of the frozen-thawed half-embryos to blastocysts in vitro were 77.8% (63/81), 82.0% (41/50), 92.1% (117/127), ...

journal_title：Molecular reproduction and development

authors： Nagashima H,Kobayashi K,Yamakawa H,Ogawa S

更新日期：1991-11-01 00:00:00

abstract：:The highly conserved Pumilio protein plays crucial roles in fertility of many organisms acting as a repressor of translation, and causing infertility when mutated. Although one of two human Pumilio homologs, PUMILIO2 is expressed mainly in the germ line, its role in mammalian germ cell development has not been reporte...

journal_title：Molecular reproduction and development

authors： Kusz K,Ginter-Matuszewska B,Ziolkowska K,Spik A,Bierla J,Jedrzejczak P,Latos-Bielenska A,Pawelczyk L,Jaruzelska J

更新日期：2007-06-01 00:00:00

abstract：:Human endometrial epithelium (EE) is composed of a multitude of proteins, amongst which those localized on the plasma membrane [plasma membrane proteins (PMPs)] are of critical relevance in the early stages of implantation. Evidence supports the key role of few PMPs in implantation. However, many remain unidentified, ...

journal_title：Molecular reproduction and development

authors： Kakar-Bhanot R,Brahmbhatt K,Kumar V,Suryawanshi AR,Srivastava S,Chaudhari U,Sachdeva G

更新日期：2020-01-01 00:00:00

abstract：:The developmental competence of bovine follicular oocytes that had been meiotically arrested with the phosphokinase inhibitor 6-dimethylaminopurine (6-DMAP) was studied. After 24 h in vitro culture with 2 mM 6-DMAP, 85 +/- 12% of the oocytes were at the germinal vesicle stage compared to 97 +/- 3% at the start of cult...

journal_title：Molecular reproduction and development

authors： Avery B,Hay-Schmidt A,Hyttel P,Greve T

更新日期：1998-07-01 00:00:00

abstract：:We previously demonstrated that the progesterone-(P) initiated human sperm acrosome reaction (AR) was dependent on the presence of extracellular Na+ (Na(-)0). Moreover, Na(-)0 depletion resulted in a decreased cytosolic pH (pHi), suggesting involvement of a Na(+)-dependent pHi regulatory mechanism during the P-initiat...

journal_title：Molecular reproduction and development

authors： Garcia MA,Meizel S

更新日期：1999-02-01 00:00:00