Abstract：
:We have developed a novel reverse transcription-polymerase chain reaction (RT-PCR)-based approach for systematically quantifying in a single experiment the abundances of many different mRNAs in preimplantation mouse embryos. With this approach, the entire mRNA population from a small number of embryos is amplified while preserving the relative abundance of each mRNA in the cDNA population. The cDNA is analyzed by quantitative hybridization to radiolabeled probes. The approach is very sensitive and provides reliable, quantitative data regarding changes in mRNA abundance. A major advantage of this method is that estimates of mRNA copy number can be obtained and compared between different mRNAs. With this approach, we analyzed the patterns of expression of nine G protein alpha subunit mRNAs (G alpha s, G alpha i, G alpha q, G alpha o, and G alpha 11-15) in oocytes, eggs, and preimplantation embryos from fertilization to the blastocyst stage. Six alpha subunit mRNAs were expressed at significant levels, all of which underwent significant temporal alterations in expression. The mRNAs encoding some alpha subunit types were expressed predominantly in the egg and 1-cell embryo, underwent sharp reductions during the 2-cell stage, and were re-expressed between the 8-cell and blastocyst stages. One alpha subunit mRNA increased in abundance at the early blastocyst stage. The possible significance of these alterations in G protein mRNA abundance to embryonic development is discussed.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Rambhatla L,Patel B,Dhanasekaran N,Latham KEdoi
10.1002/mrd.1080410306subject
Has Abstractpub_date
1995-07-01 00:00:00pages
314-24issue
3eissn
1040-452Xissn
1098-2795journal_volume
41pub_type
杂志文章abstract：:Inner cell masses (ICM) from in vitro produced day 8 or 9 bovine blastocysts were isolated by immunosurgery and cultured under different conditions in order to establish which of two feeder cell types and culture media were most efficient in supporting attachment and outgrowth of the bovine ICM cells. The efficiency o...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080400408
更新日期：1995-04-01 00:00:00
abstract：:A novel restriction fragment differential display (RFDD) RT-PCR has been used to compare patterns of mRNA expression in bovine oocytes matured in vitro in the presence (10%) or absence of fetal calf serum (FCS). Total RNA extracted from matured and denuded oocytes was processed using display Profile kit (Display Syste...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1010
更新日期：2001-05-01 00:00:00
abstract：:Hexokinase is the first enzyme in the glycolytic pathway and utilizes ATP to convert glucose to glucose-6-phosphate (G6P). We previously identified three variant transcripts of Hk1 that are expressed specifically in spermatogenic cells, have different 5' untranslated regions, and encode a protein (HK1S, spermatogenic ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20791
更新日期：2008-04-01 00:00:00
abstract：:The development of outbred mouse (CF1) zygotes in vitro has been studied using medium SOM in which the concentrations of NaCl (85, 105, 125 mM), glutamine (0, 1, 2 mM), and betaine (0, 1, 2 mM) were varied. The effects of the compounds were studied using a 3(3) factorial experimental arrangement. The inhibitory effect...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080340406
更新日期：1993-04-01 00:00:00
abstract：:The objective of this study was to examine the effect of treating pig oocytes during in vitro maturation (IVM) with a proteasome inhibitor, MG132, on oocyte maturation and embryonic development. In one series of experiments, oocytes from medium-sized follicles (3-8 mm in diameter) were untreated (MCO) or treated with ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21402
更新日期：2012-01-01 00:00:00
abstract：:We have previously shown that bovine oocytes parthenogenetically activated after 40 hours (hr) of in vitro maturation proceed through the cell cycle faster than those after 20 hr of maturation. In the present study, we used this model of different speed of nuclear progression to investigate the correlation of two hall...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10072
更新日期：2002-05-01 00:00:00
abstract：:We have examined the developmental pattern of prolactin receptor expression in the mouse by reverse transcription-polymerase chain reaction, in situ hybridization, and radioligand binding and have found two unexpected aspects of temporal regulation. First, high levels of prolactin receptor mRNA were detected in mouse ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199709)48:1<45::AID-MRD6>3
更新日期：1997-09-01 00:00:00
abstract：:Heat shock proteins (HSPs) are known to play an important role not only in various stress conditions such as exposure to heat shock, but also in normal development and/or differentiation. The role of small heat shock proteins such as HSP25 in early embryo development remains largely unknown. In the present study, we e...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1125
更新日期：2002-01-01 00:00:00
abstract：:Exposure to nickel has been shown to cause damage to the testis in several animal models. It is not known if the testis expresses protein(s) that can bind nickel. To test this, we used a nickel-binding assay to isolate testicular nickel-binding proteins. We identified glutamate-ammonia ligase (GLUL) as a prominent nic...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21275
更新日期：2011-02-01 00:00:00
abstract：:Fertile offspring from somatic cell nuclear transfer (SCNT) is the goal of most cloning laboratories. For this process to be successful, a number of events must occur correctly. First the donor nucleus must be in a state that is amenable to remodeling and subsequent genomic reprogramming. The nucleus must be introduce...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21242
更新日期：2010-12-01 00:00:00
abstract：:Teleost fish exhibit remarkably diverse and plastic patterns of sexual development. One of the most fascinating modes of plasticity is functional sex change, which is widespread in marine fish including species of commercial importance; however, the regulatory mechanisms remain elusive. In this review, we explore such...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.22691
更新日期：2017-02-01 00:00:00
abstract：:We report use of an in vitro assay (Barbato et al., 1998: Biol Reprod 58:686-699) to assess binding ability of cauda epididymal mouse sperm to a surrogate zona pellucida and effect of a synthetic peptide (Amann et al., 1999: J Androl 20: 42-46) on fertilization ability in in vitro fertilization (IVF) tests. Sperm from...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/1098-2795(200012)57:4<406::AID-MRD13>3.0.C
更新日期：2000-12-01 00:00:00
abstract：:Prolactin-like protein C (PLP-C) is a major rat placental protein which is expressed during the second half of pregnancy and belongs to the growth hormone-prolactin family. Here we report on the isolation of overlapping rat placental cDNAs which specify a transcript of 915 base pairs and predict a 205-amino acid trans...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080410207
更新日期：1995-06-01 00:00:00
abstract：:It has been proposed that the viability of early mammalian embryos is associated with a metabolism that is "quiet" rather than "active" (Leese HJ, 2002:BioEssays 24:845-849). The data on which this hypothesis was based were largely drawn from measurements on the depletion and appearance of amino acids from the culture...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.20604
更新日期：2007-10-01 00:00:00
abstract：:The purposes of this study were to establish and to explore the biological basis of the chronic stress-induced premature ovarian failure (POF) model and to explore the therapeutic effects of the traditional Chinese medicine Muniziqi. Sexually matured female Sprague-Dawley rats were fed with spinach and cilantro in col...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23092
更新日期：2019-02-01 00:00:00
abstract：:A polymorphism in the igf2 gene of chickens was identified using NlaIII (GenBank accession number AF218827). In some embryos, the igf2 alleles were expressed monoallelically from either maternal or paternal alleles. These data demonstrate that genomic imprinting is not confined to mammalian vertebrates and suggest tha...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/1098-2795(200007)56:3<345::AID-MRD3>3.0.CO
更新日期：2000-07-01 00:00:00
abstract：:Basigin, a transmembrane glycoprotein belonging to the immunoglobulin superfamily, has been shown to be essential for fertilization and implantation. The aim of this study was to determine the expression and hormonal regulation of basigin gene in mouse uterus during the peri-implantation period. Basigin immunostaining...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10128
更新日期：2002-09-01 00:00:00
abstract：:During implantation in mice, tissue inhibitor of matrix metalloproteinases-3 is believed to play a key role in inhibiting matrix metalloproteinase activity associated with embryo invasion and tissue remodeling. The first objective of this study was to quantitatively compare the steady-state mRNA levels of tissue inhib...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1018
更新日期：2001-06-01 00:00:00
abstract：:The objective of this study was to examine the effects of colcemid treatment during oocyte in vitro maturation (IVM) and embryonic development after parthenogenetic activation (PA) and somatic-cell nucleus transfer (SCNT) in pigs. Immature oocytes were treated with colcemid from 0 to 22, 38 to 42, or 0 to 22 hr follow...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22498
更新日期：2015-06-01 00:00:00
abstract：:In pigs, the morphology and cell number of in vitro-produced blastocysts are inferior to those of their in vivo counterparts. The objective of this study was to increase developmental competence and to gain an understanding of cell allocation in blastocysts derived from the aggregation of four-cell stage porcine embry...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20728
更新日期：2007-11-01 00:00:00
abstract：:In this study, we cloned and characterized the follicle stimulating hormone receptor (Fshr) and luteinizing hormone receptor (Lhr) cDNAs of pejerrey Odontesthes bonariensis, a species with temperature-dependent sex determination (TSD), and analyzed their expression in relation to Fshb and Lhb subunits during gonadogen...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21179
更新日期：2010-06-01 00:00:00
abstract：:The mRNA translational control protein, Musashi, plays a critical role in cell fate determination through sequence-specific interactions with select target mRNAs. In proliferating stem cells, Musashi exerts repression of target mRNAs to promote cell cycle progression. During stem cell differentiation, Musashi target m...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22060
更新日期：2012-08-01 00:00:00
abstract：:Testis induction is associated with gonadal Sry and Sox9 expression in mammals, and with Sox9 expression in vertebrates where Sry is absent. In mammals, Sry might initiate testis induction by upregulating Sox9 expression; however, direct evidence supporting this hypothesis is lacking. Models of Sry-negative XX sex rev...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10317
更新日期：2003-08-01 00:00:00
abstract：:The cytoplasmic beta-actin promoter, commonly used as strong promoter in many gene regulation studies, produces a pattern of male germ cell and preimplantation, embryonic gene expression in transgenic mice. In seven of ten expressing transgenic lines, a chicken beta-actin-lacZ fusion gene was expressed in adult testes...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080340202
更新日期：1993-02-01 00:00:00
abstract：:Tcp-11 is a candidate for a distorter gene within the t-complex on mouse chromosome 17; although t-complex genes appear to affect sperm function, relatively little is known about mechanisms whereby these genes might play a specific physiological role. We present evidence that the protein TCP-11 is found on the surface...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199711)48:3<375::AID-MRD11
更新日期：1997-11-01 00:00:00
abstract：:Artificial activation is required for successful intracytoplasmic sperm injection (ICSI) to induce haploidy pronuclear formation with extraction of second polar body. The present study showed that an additional treatment with Phorbol 12-myristate 13-acetate (PMA) followed by Ca(2+) ionophore treatment improved the rat...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20182
更新日期：2005-01-01 00:00:00
abstract：:Izumo, a sperm membrane protein, is essential for gamete fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N-terminal domain for which neither the functions nor homologous sequences are known. In the present work we identified three novel proteins showing an N-terminal domain with significant homology t...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21092
更新日期：2009-12-01 00:00:00
abstract：:Abnormal oocyte spindle due to the improper function of ooplasm is associated with female infertility of advanced maternal age. A possible way to overcome this problem is to transfer an oocyte germinal vesicle (GV) which contains genetic materials of a patient with a history of poor embryo development to the cytoplast...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/1098-2795(200102)58:2<180::AID-MRD7>3.0.CO
更新日期：2001-02-01 00:00:00
abstract：:Exposed plasma membrane proteins were labeled with 125I before and after incubation of boar sperm under capacitating conditions. Labeled protein profiles were compared to the ability of the sperm to penetrate zona-free hamster ova. Quantitatively, the labeled sperm membrane proteins were primarily low Mr prior to capa...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080270311
更新日期：1990-11-01 00:00:00
abstract：:The analysis of differences in gene expression, responding to cryopreservation may explain some of the observed differences in further development of the preimplantation stage embryos. The aim of this study was to create a link, for the first time, between morphological/developmental observations and gene activity cha...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20450
更新日期：2006-06-01 00:00:00
