Analysis of G protein alpha subunit mRNA abundance in preimplantation mouse embryos using a rapid, quantitative RT-PCR approach.

Abstract:

:We have developed a novel reverse transcription-polymerase chain reaction (RT-PCR)-based approach for systematically quantifying in a single experiment the abundances of many different mRNAs in preimplantation mouse embryos. With this approach, the entire mRNA population from a small number of embryos is amplified while preserving the relative abundance of each mRNA in the cDNA population. The cDNA is analyzed by quantitative hybridization to radiolabeled probes. The approach is very sensitive and provides reliable, quantitative data regarding changes in mRNA abundance. A major advantage of this method is that estimates of mRNA copy number can be obtained and compared between different mRNAs. With this approach, we analyzed the patterns of expression of nine G protein alpha subunit mRNAs (G alpha s, G alpha i, G alpha q, G alpha o, and G alpha 11-15) in oocytes, eggs, and preimplantation embryos from fertilization to the blastocyst stage. Six alpha subunit mRNAs were expressed at significant levels, all of which underwent significant temporal alterations in expression. The mRNAs encoding some alpha subunit types were expressed predominantly in the egg and 1-cell embryo, underwent sharp reductions during the 2-cell stage, and were re-expressed between the 8-cell and blastocyst stages. One alpha subunit mRNA increased in abundance at the early blastocyst stage. The possible significance of these alterations in G protein mRNA abundance to embryonic development is discussed.

journal_name

Mol Reprod Dev

authors

Rambhatla L,Patel B,Dhanasekaran N,Latham KE

doi

10.1002/mrd.1080410306

subject

Has Abstract

pub_date

1995-07-01 00:00:00

pages

314-24

issue

3

eissn

1040-452X

issn

1098-2795

journal_volume

41

pub_type

杂志文章
  • Direct evidence that the mouse sex-determining gene Sry is expressed in the somatic cells of male fetal gonads and in the germ cell line in the adult testis.

    abstract::Reverse transcriptase polymerase chain reaction (RT-PCR) analysis shows that Sry mRNA is expressed in male fetal urogenital ridges from 12.5 day p.c. embryos, but not in enriched populations of primordial germ cells from the same embryos, indicating that Sry is expressed in the somatic cells of the embryonal gonad at ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080340404

    authors: Rossi P,Dolci S,Albanesi C,Grimaldi P,Geremia R

    更新日期:1993-04-01 00:00:00

  • Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells.

    abstract::Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the u...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22771

    authors: Secher JO,Ceylan A,Mazzoni G,Mashayekhi K,Li T,Muenthaisong S,Nielsen TT,Li D,Li S,Petkov S,Cirera S,Luo Y,Thombs L,Kadarmideen HN,Dinnyes A,Bolund L,Roelen BA,Schmidt M,Callesen H,Hyttel P,Freude KK

    更新日期:2017-03-01 00:00:00

  • PLAC1 expression increases during trophoblast differentiation: evidence for regulatory interactions with the fibroblast growth factor-7 (FGF-7) axis.

    abstract::PLAC1 is a recently described, trophoblast-specific gene that localizes to a region of the X-chromosome important in placental development. Immunohistochemical analysis demonstrated that PLAC1 polypeptide localizes to the differentiated syncytiotrophoblast throughout gestation (8-41 weeks) as well as a small populatio...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20272

    authors: Massabbal E,Parveen S,Weisoly DL,Nelson DM,Smith SD,Fant M

    更新日期:2005-07-01 00:00:00

  • Aberrant methylation of the IL-12B promotor region contributes to the risk of developing ovarian endometriosis.

    abstract::Studies have shown that aberrant expression of IL-12p40, which is encoded by the interleukin-12B (IL-12B) gene, may be involved in the development of endometriosis. In this study, we investigated the role of aberrant methylation of the IL-12B promoter region and its associated expression in the development of ovarian ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.23139

    authors: Zhao W,Kang S,Zhao J,Wang L,Cao S,Li Y

    更新日期:2019-06-01 00:00:00

  • Haprin-deficient spermatozoa are incapable of in vitro fertilization.

    abstract::Haprin (TRIM36) is a ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. It is expressed in the testes in both mice and humans and is thought to be involved in spermiogenesis, the acrosome reaction, and fertilization. However, the functional role of Haprin i...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.23344

    authors: Aoki Y,Tsujimura A,Kaseda K,Okabe M,Tokuhiro K,Ohta T,O'Bryan MK,Okuda H,Kitamura K,Ogawa Y,Fujiki T,Wada M,Horie S,Nishimune Y,Tanaka H

    更新日期:2020-05-01 00:00:00

  • Nuclear transplantation in bovine embryo: fine structural and autoradiographic studies.

    abstract::Nucleolar fine structure, "blebbing" activity of nuclear envelope, and activation of heterogeneous nuclear RNA (hnRNA) synthesis were studied in bovine reconstructed embryos obtained by electrofusion of a single eight-cell blastomere with an enucleated oocyte. Developmental progress of nucleolar fine structure and hnR...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080290204

    authors: Kanka J,Fulka J Jr,Fulka J,Petr J

    更新日期:1991-06-01 00:00:00

  • Role of adiponectin in delayed embryonic development of the short-nosed fruit bat, Cynopterus sphinx.

    abstract::The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22425

    authors: Anuradha,Krishna A

    更新日期:2014-12-01 00:00:00

  • Follicle-oocyte atresia and temporal taphonomy in cold-stored domestic cat ovaries.

    abstract::In vitro oocyte maturation followed by in vitro fertilization (IVM/IVF) success in the domestic cat remains inferior to commonly studied livestock or laboratory species. The objectives here were (1) to histologically assess atresia status of freshly excised follicle/oocyte complexes, and (2) to evaluate taphonomic cha...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2795(199702)46:2<190::AID-MRD9>

    authors: Wood TC,Montali RJ,Wildt DE

    更新日期:1997-02-01 00:00:00

  • Evolution of octopod sperm I: comparison of nuclear morphogenesis in Eledone and Octopus.

    abstract::Morphogenesis of the Eledone cirrhosa sperm nucleus, as studied by electron microscopic techniques, is compared with that of Octopus vulgaris. Both species of cephalopods belong to the family Octopodidae. The results indicate that extensive nuclear helicoidization during E. cirrhosa spermiogenesis is brought about by ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.10124

    authors: Gimenez-Bonafé P,Ribes E,Zamora MJ,Kasinsky HE,Chiva M

    更新日期:2002-07-01 00:00:00

  • Suppression of translation during in vitro maturation of pig oocytes despite enhanced formation of cap-binding protein complex eIF4F and 4E-BP1 hyperphosphorylation.

    abstract::In this study, we document that the overall rate of protein synthesis decreases during in vitro maturation (IVM) of pig oocytes despite enhanced formation of the 5' cap structure eIF4F. Within somatic/interphase cells, formation of the eIF4F protein complex correlates very well with overall rates of protein translatio...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20368

    authors: Ellederova Z,Kovarova H,Melo-Sterza F,Livingstone M,Tomek W,Kubelka M

    更新日期:2006-01-01 00:00:00

  • Viable rabbits derived from reconstructed oocytes by germinal vesicle transfer after intracytoplasmic sperm injection (ICSI).

    abstract::Abnormal oocyte spindle due to the improper function of ooplasm is associated with female infertility of advanced maternal age. A possible way to overcome this problem is to transfer an oocyte germinal vesicle (GV) which contains genetic materials of a patient with a history of poor embryo development to the cytoplast...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/1098-2795(200102)58:2<180::AID-MRD7>3.0.CO

    authors: Li GP,Chen DY,Lian L,Sun QY,Wang MK,Liu JL,Li JS,Han ZM

    更新日期:2001-02-01 00:00:00

  • Molecular cloning and characterization of SRG-L, a novel mouse gene developmentally expressed in spermatogenic cells.

    abstract::Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20485

    authors: Ma Q,Wang H,Guo R,Wang H,Ge Y,Ma J,Xue S,Han D

    更新日期:2006-09-01 00:00:00

  • Developmental arrest induced in cleavage stage porcine embryos following microinjection of mRNA encoding Brahma (Smarca 2), a chromatin remodeling protein.

    abstract::Smarca 2 (Brahma) and Smarca 4 (Brahma related gene 1, BRG1) alternatively occupy the catalytic site of SWI/SNF chromatin remodeling complexes. Mammalian embryos undergo a dramatic amount of epigenetic remodeling during cleavage development, which plays key roles in regulating both gene transcription and the developme...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20710

    authors: Magnani L,Cabot RA

    更新日期:2007-10-01 00:00:00

  • Expression of Cu/Zn and Mn superoxide dismutases during bovine embryo development: influence of in vitro culture.

    abstract::Temporal pattern of expression of Cu/Zn and Mn superoxide dismutases (SODs) was investigated in bovine oocytes and embryos produced in vitro in two different culture conditions and in vivo after superovulation. SODs were examined at a transcriptional level in single oocytes and embryos by reverse transcriptase-polymer...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/1098-2795(200101)58:1<45::AID-MRD7>3.0.CO;

    authors: Lequarré AS,Feugang JM,Malhomme O,Donnay I,Massip A,Dessy F,Van Langendonckt A

    更新日期:2001-01-01 00:00:00

  • Bovine mater-like NALP9 is an oocyte marker gene.

    abstract::We have cloned and sequenced the bovine NALP9 cDNA, which contains a 2991 bp long open reading frame with 76% homology to its human counterpart. The putative 996 amino acids protein presents the domain organization typical of the Nacht, Leucine rich repeat and Pyrin domain containing (NALP) family that includes the ma...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20298

    authors: Dalbiès-Tran R,Papillier P,Pennetier S,Uzbekova S,Monget P

    更新日期:2005-08-01 00:00:00

  • Non-hormonal targets underlying endometriosis: A focus on molecular mechanisms.

    abstract::Endometriosis is regarded as a hormone-dependent disease. Current therapeutic approaches to treating this common gynecological disorder mainly depend on surgical and hormonal interventions, but the high rate of disease recurrence as well as the side effects related to such therapies make it difficult for patients to r...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审

    doi:10.1002/mrd.22493

    authors: Che XH,Chen YC,Chen CL,Ye XL,Zhu H

    更新日期:2015-06-01 00:00:00

  • BH4 peptide derived from Bcl-xL and Bax-inhibitor peptide suppresses apoptotic mitochondrial changes in heat stressed bovine oocytes.

    abstract::Mitochondria play an important role in the integration and transmission of cell death signals mediated by the Bcl-2 family proteins. Experiments were conducted to determine whether the anti-apoptotic peptides BH4 domain of Bcl-xL (TAT-BH4) and Bax inhibitor peptide (BIP) suppresses heat stress (HS) injury in oocytes b...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20986

    authors: Soto P,Smith LC

    更新日期:2009-07-01 00:00:00

  • Association of MPF, MAPK, and nuclear progression dynamics during activation of young and aged bovine oocytes.

    abstract::We have previously shown that bovine oocytes parthenogenetically activated after 40 hours (hr) of in vitro maturation proceed through the cell cycle faster than those after 20 hr of maturation. In the present study, we used this model of different speed of nuclear progression to investigate the correlation of two hall...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.10072

    authors: Tian XC,Lonergan P,Jeong BS,Evans AC,Yang X

    更新日期:2002-05-01 00:00:00

  • Heat stress, a serious threat to reproductive function in animals and humans.

    abstract::Global warming represents a major stressful environmental condition that compromises the reproductive efficiency of animals and humans via a rise of body temperature above its physiological homeothermic point (heat stress [HS]). The injuries caused by HS on reproductive function involves both male and female component...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审

    doi:10.1002/mrd.23123

    authors: Boni R

    更新日期:2019-10-01 00:00:00

  • Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): evidence for vasa localization at cleavage furrows in euteleostei.

    abstract::Specification of primordial germ cells during early embryogenesis is a critical biological issue in reproduction and development. Yet, little is known in marine economic fish species. Vasa, a component of germ plasm, is the most-documented germ cell marker in teleosts. We isolated a full-length vasa cDNA (Smvas) from ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22120

    authors: Lin F,Xu S,Ma D,Xiao Z,Zhao C,Xiao Y,Chi L,Liu Q,Li J

    更新日期:2012-11-01 00:00:00

  • Expression of homeobox-containing genes in cDNA libraries derived from cattle oocytes and preimplantation stage embryo.

    abstract::The homeobox-containing gene family plays a pivotal role in regulating, patterning, and axial morphogenesis in the developing embryo. But there is still very little known about the expression and function of these genes in mammalian oocytes and preimplantation stage embryos. In this study we have used degenerate prime...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1091

    authors: Ponsuksili S,Wimmers K,Adjaye J,Schellander K

    更新日期:2001-11-01 00:00:00

  • Mouse preimplantation embryo development in vitro: effect of sodium concentration in culture media on RNA synthesis and accumulation and gene expression.

    abstract::Results of previous studies indicate that culture of preimplantation mouse embryos in SOM medium containing 85 mM NaCl promotes better development in vitro, as well as supporting higher rates of protein synthesis, when compared to culture in SOM containing 125 mM NaCl (Anbari and Schultz, 1993, Mol Reprod Dev 35:24-28...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080380203

    authors: Ho Y,Doherty AS,Schultz RM

    更新日期:1994-06-01 00:00:00

  • Stage-dependent and alternative splicing of sGnRH messengers in rainbow trout testis during spermatogenesis.

    abstract::The gonadotropin releasing hormone (GnRH) has long been considered as a neuropeptide involved in the control of the reproductive cycle. However, the presence of GnRH and its receptors in various tissues, including ovary and testis, suggests a role as autocrine/paracrine factor. In the present study, we report the expr...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1000

    authors: Uzbekova S,Ferrière F,Guiguen Y,Bailhache T,Breton B,Lareyre JJ

    更新日期:2001-05-01 00:00:00

  • Reorganization of mouse sperm lipid rafts by capacitation.

    abstract::One of the hallmarks of mammalian sperm capacitation is the loss of cholesterol from the plasma membrane. Cholesterol has been associated with the formation of detergent insoluble membrane microdomains in many cell types, and sperm from several mammalian species have been shown to contain detergent-resistant membranes...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20540

    authors: Thaler CD,Thomas M,Ramalie JR

    更新日期:2006-12-01 00:00:00

  • The sensitive period for male-to-female sex reversal begins at the embryonic stage in the Nile tilapia and is associated with the sexual genotype.

    abstract::In this study, we sought to determine the mechanism of early sex reversal in a teleost by applying 4 hr feminization treatments to XY (17α-ethynylestradiol 2000 μg L(-1) ) and YY (6500 μg L(-1) ) Nile tilapia embryos on the first day post-fertilization (dpf). We then searched for changes in the expression profiles of ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22436

    authors: Gennotte V,Mélard C,D'Cotta H,Baroiller JF,Rougeot C

    更新日期:2014-12-01 00:00:00

  • Cyclin A2 is phosphorylated during the G2/M transition in mouse two-cell embryos.

    abstract::In the present study, we investigated the expression of cyclin A2 in mouse two-cell embryos to elucidate the role of cyclin A2 at the G2/M transition. Two forms of cyclin A2 on SDS-PAGE (an upper and a lower band) were detected in two-cell embryos synchronized at the M phase by nocodazole. To investigate the nature of...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.10366

    authors: Ohashi A,Imai H,Minami N

    更新日期:2003-12-01 00:00:00

  • Plac1 (placenta-specific 1) is essential for normal placental and embryonic development.

    abstract::Plac1 is a recently identified, X-linked gene whose expression is restricted primarily to cells of the trophoblast lineage. It localizes to a chromosomal locus previously implicated in placental growth. We therefore sought to determine if Plac1 is necessary for placental and embryonic development by examining a mutant...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22062

    authors: Jackman SM,Kong X,Fant ME

    更新日期:2012-08-01 00:00:00

  • Sex determination and gender expression: Reproductive investment in snails.

    abstract::Sex determination is generally seen as an issue of importance for separate-sexed organisms; however, when considering other sexual systems, such as hermaphroditism, sex allocation is a less-binary form of sex determination. As illustrated here, with examples from molluscs, this different vantage point can offer import...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审

    doi:10.1002/mrd.22662

    authors: Koene JM

    更新日期:2017-02-01 00:00:00

  • Isolation and characterization of permanent cell lines from inner cell mass cells of bovine blastocysts.

    abstract::Inner cell masses (ICM) from in vitro produced day 8 or 9 bovine blastocysts were isolated by immunosurgery and cultured under different conditions in order to establish which of two feeder cell types and culture media were most efficient in supporting attachment and outgrowth of the bovine ICM cells. The efficiency o...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080400408

    authors: Van Stekelenburg-Hamers AE,Van Achterberg TA,Rebel HG,Fléchon JE,Campbell KH,Weima SM,Mummery CL

    更新日期:1995-04-01 00:00:00

  • Centrosome reduction during Rhesus spermiogenesis: gamma-tubulin, centrin, and centriole degeneration.

    abstract::Centrosome reduction during spermiogenesis has been studied using anti-gamma-tubulin and anti-centrin antibodies and electron microscopy in nonhuman primates. Rhesus spermatids possess apparently normal centrosomes comprising a pair of centrioles associated with gamma-tubulin and centrin. However, they do not nucleate...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/1098-2795(200008)56:4<502::AID-MRD8>3.0.CO

    authors: Manandhar G,Schatten G

    更新日期:2000-08-01 00:00:00