Abstract：
:We have developed a novel reverse transcription-polymerase chain reaction (RT-PCR)-based approach for systematically quantifying in a single experiment the abundances of many different mRNAs in preimplantation mouse embryos. With this approach, the entire mRNA population from a small number of embryos is amplified while preserving the relative abundance of each mRNA in the cDNA population. The cDNA is analyzed by quantitative hybridization to radiolabeled probes. The approach is very sensitive and provides reliable, quantitative data regarding changes in mRNA abundance. A major advantage of this method is that estimates of mRNA copy number can be obtained and compared between different mRNAs. With this approach, we analyzed the patterns of expression of nine G protein alpha subunit mRNAs (G alpha s, G alpha i, G alpha q, G alpha o, and G alpha 11-15) in oocytes, eggs, and preimplantation embryos from fertilization to the blastocyst stage. Six alpha subunit mRNAs were expressed at significant levels, all of which underwent significant temporal alterations in expression. The mRNAs encoding some alpha subunit types were expressed predominantly in the egg and 1-cell embryo, underwent sharp reductions during the 2-cell stage, and were re-expressed between the 8-cell and blastocyst stages. One alpha subunit mRNA increased in abundance at the early blastocyst stage. The possible significance of these alterations in G protein mRNA abundance to embryonic development is discussed.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Rambhatla L,Patel B,Dhanasekaran N,Latham KEdoi
10.1002/mrd.1080410306subject
Has Abstractpub_date
1995-07-01 00:00:00pages
314-24issue
3eissn
1040-452Xissn
1098-2795journal_volume
41pub_type
杂志文章abstract：:The IGFs (IGF-I and IGF-II) are essential for normal mammalian growth and development. Their actions are mediated primarily by their interactions with the type I IGF receptor (IGF-I receptor), a transmembrane tyrosine kinase. The ligands and the IGF-I receptor are structurally related to insulin and to the insulin rec...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.1080350403
更新日期：1993-08-01 00:00:00
abstract：:Apoptosis is a well-established cellular mechanism for selective cell deletion during development. However, little is known about the expression of an apoptotic pathway and its role in determining the relative sensitivity of the early, pre-gastrula, avian embryo to stress-induced cell death. We examined the sensitivit...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199810)51:2<130::AID-MRD2>
更新日期：1998-10-01 00:00:00
abstract：:The present studies were performed to establish the effects of the size and number of artificial holes produced in the zona pellucida (ZP) on hatching and trophoblast outgrowth in vitro. Limited partial zona dissection (PZD) produced small, narrow incisions, and zona drilling with acidic Tyrode's (AT) across a larger ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080300110
更新日期：1991-09-01 00:00:00
abstract：:Tcp-11 is a candidate for a distorter gene within the t-complex on mouse chromosome 17; although t-complex genes appear to affect sperm function, relatively little is known about mechanisms whereby these genes might play a specific physiological role. We present evidence that the protein TCP-11 is found on the surface...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199711)48:3<375::AID-MRD11
更新日期：1997-11-01 00:00:00
abstract：:Differential expression of genes leads to variations in the phenotypes of X and Y sperm, although some differentially expressed gene products are shared through intercellular bridges. Genes differentially expressed in bovine X and Y sperm were identified by a combination of suppression subtractive hybridization (SSH),...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22386
更新日期：2014-10-01 00:00:00
abstract：:In this study, a combination of RNAi and endoscopic transfer to the oviduct of synchronized heifers has been used to investigate the effect of suppression of Cx43 and E-cadherin on the development, mRNA and protein expression of bovine blastocysts cultured in vitro or in vivo. In vitro matured and fertilized bovine zy...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20678
更新日期：2007-08-01 00:00:00
abstract：:Activation of the mammalian egg results in cortical reaction (CR), which is correlated with an increase in intracellular Ca2+ concentration and PKC activation. The CR is a gradual rather then an "all or none" response, and can be regulated by different concentrations of parthenogenetic activators. To evaluate the biol...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199811)51:3<295::AID-MRD9>
更新日期：1998-11-01 00:00:00
abstract：:In this review, we discuss the expression, regulation, downstream mechanisms, and function of stress-induced stress enzymes in mammalian oocytes, peri-implantation embryos, and the stem cells derived from those embryos. Recent reports suggest that stress enzymes mediate developmental functions during early mammalian d...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.20787
更新日期：2008-04-01 00:00:00
abstract：:Selection of transgenic embryos prior to embryo transfer is a means to increase the efficiency of transgenic livestock production. Among transgenic reporters, cytoplasmic expression of green fluorescent protein (GFP) has features that make it ideal for transgenic embryo selection. The primary objective of this study w...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199904)52:4<406::AID-MRD9>
更新日期：1999-04-01 00:00:00
abstract：:Sperm-mediated gene transfer in vertebrates has undergone various developments over the last few years, in different laboratories. In the present study, we microinjected a circular plasmid, carrying the lacZ reporter gene mixed with noncommercial cationic lipids, into the seminiferous tubules of anesthetized adult mic...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10143
更新日期：2002-08-01 00:00:00
abstract：:Forward-motility-stimulating factor (FMSF) is a protein, originally purified from bubaline serum, that promotes progressive motility of mature spermatozoa. FMSF binds to sperm surface receptors and activates transmembrane adenylyl cyclase (tmAC), causing a rise in intracellular cyclic AMP level ([cAMP]i) and subsequen...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22586
更新日期：2015-12-01 00:00:00
abstract：:Oocyte maturation is accompanied by differentiation of surrounding cumulus cells. These cells produce hyaluronic acid (HA) and its storage in intercellular spaces results in expansion of the cells. The cumulus cells also accumulate cyclooxygenase-2 (cox-2) during maturation. Both expansion and cox-2 storage are regula...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10265
更新日期：2003-05-01 00:00:00
abstract：:The murine intracisternal A particle (IAP) proviral elements are expressed at low levels in undifferentiated F9 embryonal carcinoma cells but are highly expressed when F9 cells are induced to differentiate into parietal endoderm-like cells. IAP elements are also expressed in parietal endoderm-like PYS-2 cells. We prev...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080410103
更新日期：1995-05-01 00:00:00
abstract：:The genome of germline committed cells is thought to be protected by mechanisms of transcriptional silencing, posing a barrier to transgenesis using cultured germline cells. We found that selection for transgene integration into the primordial germ cell genome required that the transgenes be flanked by the chicken bet...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20859
更新日期：2008-07-01 00:00:00
abstract：:Hexokinase is the first enzyme in the glycolytic pathway and utilizes ATP to convert glucose to glucose-6-phosphate (G6P). We previously identified three variant transcripts of Hk1 that are expressed specifically in spermatogenic cells, have different 5' untranslated regions, and encode a protein (HK1S, spermatogenic ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20791
更新日期：2008-04-01 00:00:00
abstract：:Infusion of seminal plasma in the uterus is known to elicit an instant inflammatory response in the porcine uterus, but whether or not it prepares a uterine immunological response to the presence of conceptuses is not well understood. Seminal plasma induced long-term modulatory effects and conceptus-induced immune cha...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22297
更新日期：2014-03-01 00:00:00
abstract：:Global warming represents a major stressful environmental condition that compromises the reproductive efficiency of animals and humans via a rise of body temperature above its physiological homeothermic point (heat stress [HS]). The injuries caused by HS on reproductive function involves both male and female component...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.23123
更新日期：2019-10-01 00:00:00
abstract：:This study was carried out to examine the participation of epidermal growth factor (EGF)-like peptides in the induction of germinal vesicle breakdown (GVB) in mouse cumulus cell-enclosed oocytes (CEO). The EGF-like peptide, amphiregulin (AR), dose-dependently stimulated meiotic resumption in CEO, but not denuded oocyt...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20781
更新日期：2008-01-01 00:00:00
abstract：:Treatment of in vitro matured bovine oocytes with colcemid results in a membrane protrusion that contains maternal chromosomes, which can be easily removed by aspiration. Four experiments were designed to evaluate the overall and temporal effects of conditioned medium (CM) by bovine cumulus cells on development of nuc...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20071
更新日期：2004-06-01 00:00:00
abstract：:RNase MRP is a ribonucleoprotein endoribonuclease found predominantly in nucleoli, but which has been associated with mitochondria and mitochondrial RNA processing. In order to analyze the intracellular localization of specific RNA components of ribonucleoproteins of this type, a whole-mount method for in situ hybridi...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080420313
更新日期：1995-11-01 00:00:00
abstract：:Embryonic stem (ES) cell lines are routinely derived from in vivo produced blastocysts. We investigated the efficiency of ES cells derivation from in vitro produced blastocysts either in monoculture or sequential culture. Zygotes from hybrid F1 B6D2 mice were cultured in vitro to the blastocyst stage in Potassium (K(+...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20512
更新日期：2006-08-01 00:00:00
abstract：:Epigenetics includes the study of external factors that can influence the expression of genes by altering the accessibility of DNA through methylation. To investigate the epigenetic influence of season, sperm head shape, and semen storage on placental and fetal tissues, pregnancies were generated in the summer or wint...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23125
更新日期：2019-04-01 00:00:00
abstract：:We have cloned and sequenced cDNAs encoding autoantigen 1 (AA1), a testis-specific protein and the major autoantigen of the guinea pig sperm acrosome. The cDNA predicts a precursor protein of 244 amino acids including a 21 amino acid hydrophobic, secretory signal sequence. The mature polypeptide is predicted to have a...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199606)44:2<221::AID-MRD11
更新日期：1996-06-01 00:00:00
abstract：:Studies of ovine interferon-tau (oIFNtau) gene regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, have been carried out, but a definitive mechanism for its spatial-temporal transcription has not been elucidated. Recently, specific binding regions for transcription factors AP-1 and...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10293
更新日期：2003-05-01 00:00:00
abstract：:The aim of the present study was to investigate the mechanism of androgen-mediated, prolonged sperm-storage in the female reproductive tract of the bat, Scotophilus heathii. The bat treated in vivo with flutamide, an androgen antagonist, showed loss of spermatozoa at the storage site, utero-tubal junction. Immunocytoc...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21328
更新日期：2011-07-01 00:00:00
abstract：:The present study was conducted to reveal effects of in ovo injection of nonsteroidal aromatase inhibitor (Fadrozole) or estradiol at day 3 of incubation on mRNA levels of P45017alphahydroxylase (P450c17), P450 aromatase (P450arom) and anti-Müllerian hormone (AMH) in the chicken gonads. The mRNA levels in the gonads a...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(200001)55:1<20::AID-MRD4>3
更新日期：2000-01-01 00:00:00
abstract：:The frameworks (key mechanisms) of the self/non-self-discrimination systems that are found in various organisms have not been actively selected for, but have evolved by genetic drift such that the genetic frequency of random, advantageous mutations has increased within the genomes of these species by natural selection...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22134
更新日期：2013-01-01 00:00:00
abstract：:Mammalian reproductive processes involve spermatogenesis, which occurs in the testis, and fertilization, which takes place in the female genital tract. Fertilization is a successive, multistep, and extremely complicated event that usually includes sperm survival in the uterus, sperm migration through the uterotubal ju...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.23180
更新日期：2019-08-01 00:00:00
abstract：:A method for assessment of the human sperm acrosome reaction is reported using fluorescein isothiocyanate (FITC)-conjugated Concanavalin A (ConA). The technique involved labelling prefixed spermatozoa, where only those spermatozoa that showed a complete loss of the acrosome bound FITC-ConA to the acrosomal region. Com...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080250306
更新日期：1990-03-01 00:00:00
abstract：:Aluminum hydroxide gel (Al-gel), which is used as an adjuvant, can absorb macromolecules. We investigated the applicability of Al-gel to the sustained release of follicle-stimulating hormone (FSH) as a simplified method of superovulation (SOV) in rabbits. The responsiveness of rabbits to SOV by a single injection of F...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20672
更新日期：2007-09-01 00:00:00
