Abstract:
:In this study, we cloned and characterized the follicle stimulating hormone receptor (Fshr) and luteinizing hormone receptor (Lhr) cDNAs of pejerrey Odontesthes bonariensis, a species with temperature-dependent sex determination (TSD), and analyzed their expression in relation to Fshb and Lhb subunits during gonadogenesis at temperatures producing only females (17 degrees C, FPT), both sexes (25 degrees C, MixPT), and only males (29 degrees C, MPT). The pejerrey Fshr cDNA had 3,069 bp for a mature protein of 694 amino acids (aa) and a signal peptide of 22 aa; the Lhr cDNA had 2,936 bp for a mature protein of 676 aa and a signal peptide of 25 aa. With the exception of Lhr in fish at the MPT, all genes showed significant increases and/or peaks of expression before histological differentiation of the gonads regardless of temperature. Larvae at the FPT had lower Fshb and Lhb but higher Lhr expression during the TSD period than those at the MPT; a clear pattern could not be ascertained for Fshr. At the MixPT, Fshb, Lhb, and Lhr mRNA increased in approximately half of the fish during TSD and sex differentiation and the sex ratio was 55.2% male. Based on the above results, it is suggested that animals with high Fshb and Lhb and low Lhr values represent putative males. These evidences, together with other studies, suggest that temperature may signal through the pituitary (differential expression of Fshb and Lhb) down to the gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis during the TSD process of pejerrey.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Shinoda T,Miranda LA,Okuma K,Hattori RS,Fernandino JI,Yoshizaki G,Somoza GM,Strüssmann CAdoi
10.1002/mrd.21179subject
Has Abstractpub_date
2010-06-01 00:00:00pages
521-32issue
6eissn
1040-452Xissn
1098-2795journal_volume
77pub_type
杂志文章abstract::Previous studies have shown that in adult male mice, expression of the meg1 gene is restricted to meiotic and early postmeiotic testicular germ cells. We have now analyzed the expression of meg1 during postnatal testicular development and the comparable meiotic stages in the female. The 0.75 kb transcript for meg1 beg...
journal_title:Molecular reproduction and development
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更新日期:1996-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1994-07-01 00:00:00
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
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