Abstract:
:Exposure to nickel has been shown to cause damage to the testis in several animal models. It is not known if the testis expresses protein(s) that can bind nickel. To test this, we used a nickel-binding assay to isolate testicular nickel-binding proteins. We identified glutamate-ammonia ligase (GLUL) as a prominent nickel-binding protein by mass spectrometry. Protein analysis and reverse transcriptase polymerase chain reaction showed that GLUL is expressed in the testis, predominantly in interstitial cells. We determined that GLUL has a higher affinity for nickel than for its regular co-factor manganese. We produced an enzymatically active, recombinant GLUL protein. Upon binding, nickel interferes with the manganese-catalyzed enzymatic activity of recombinant GLUL protein. We also determined that GLUL activity in testes of animals exposed to nickel sulfate is reduced. Our results identify testicular GLUL as the first testicular protein shown to be affected by nickel exposure.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Sun Y,Ou Y,Cheng M,Ruan Y,van der Hoorn FAdoi
10.1002/mrd.21275subject
Has Abstractpub_date
2011-02-01 00:00:00pages
104-15issue
2eissn
1040-452Xissn
1098-2795journal_volume
78pub_type
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journal_title:Molecular reproduction and development
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