Abstract:
:In the present study, we investigated the expression of cyclin A2 in mouse two-cell embryos to elucidate the role of cyclin A2 at the G2/M transition. Two forms of cyclin A2 on SDS-PAGE (an upper and a lower band) were detected in two-cell embryos synchronized at the M phase by nocodazole. To investigate the nature of this shift, embryos synchronized at the M phase were treated with alkaline phosphatase (AP). The upper band of cyclin A2 was fainter in AP-treated embryos than in nontreated embryos. This result indicates that cyclin A2 in mouse two-cell embryos is phosphorylated and the band on SDS-PAGE shifts up during the G2/M transition. In addition, we examined the sequential expression of cyclin A2 in two-cell blocked embryos after OA treatment. The upper band of cyclin A2 was first detected at 2 hr after the treatment, corresponding to the timing of Cdc2 kinase activation. In two-cell embryos after removal from nocodazole treatment, the phosphorylated form of cyclin A2 protein decreased abruptly just before cytokinesis. These results suggest that the mechanism of cyclin A2 degradation in mouse two-cell embryos may be different from that in somatic cells.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Ohashi A,Imai H,Minami Ndoi
10.1002/mrd.10366keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
343-8issue
4eissn
1040-452Xissn
1098-2795journal_volume
66pub_type
杂志文章abstract::We hypothesized that the testis-specific isoform of angiotensin-converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti-tACE antibody; (ii) characterize expression of tACE in bovine tes...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22790
更新日期:2017-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 评论,杂志文章
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更新日期:2001-04-01 00:00:00
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更新日期:2000-03-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10396
更新日期:2004-02-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1990-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080400408
更新日期:1995-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.21328
更新日期:2011-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199712)48:4<458::AID-MRD6>
更新日期:1997-12-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330308
更新日期:1992-11-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22131
更新日期:2013-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420303
更新日期:1995-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.21263
更新日期:2011-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23125
更新日期:2019-04-01 00:00:00
abstract::Fertilization in Bufo arenarum requires the sperm to penetrate the egg envelopes. The incubation of isolated vitelline envelopes with sperm induces the acrosome reaction, releasing proteases and glycosidases to the media. In the present work N-acetyl-beta-D-glucosaminidase, beta-D-galactosidase, beta-D-glucosidase, al...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200010)57:2<194::AID-MRD11>3.0.C
更新日期:2000-10-01 00:00:00
abstract::Meiotic progression requires the translation of maternal mRNAs in a strict temporal order. In isolated animal oocytes, translation of maternal mRNAs containing a cytoplasmic polyadenylation element (CPE), such as cyclin B, is activated by in vitro stimulation of meiotic resumption which induces phosphorylation of CPEB...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2016-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1998-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199907)53:3<294::AID-MRD5>
更新日期:1999-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200006)56:2+<301::AID-MRD2
更新日期:2000-06-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080360212
更新日期:1993-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200012)57:4<406::AID-MRD13>3.0.C
更新日期:2000-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22586
更新日期:2015-12-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199902)52:2<189::AID-MRD10
更新日期:1999-02-01 00:00:00