Block of CDK1-dependent polyadenosine elongation of Cyclin B mRNA in metaphase-i-arrested starfish oocytes is released by intracellular pH elevation upon spawning.

Abstract:

:Meiotic progression requires the translation of maternal mRNAs in a strict temporal order. In isolated animal oocytes, translation of maternal mRNAs containing a cytoplasmic polyadenylation element (CPE), such as cyclin B, is activated by in vitro stimulation of meiotic resumption which induces phosphorylation of CPEB (CPE-binding protein) and elongation of their polyadenosine (poly(A)) tails; whether or not this model can be applied in vivo to oocytes arrested at metaphase of meiosis I in ovaries is unknown. In this study, we found that active CDK1 (cyclin-dependent kinase 1) phosphorylated CPEB in ovarian oocytes arrested at metphase I in the starfish body cavity, but phosphorylation of CPEB was not sufficient for elongation of cyclin B poly(A) tails. Immediately after spawning, however, mRNA was polyadenylated, suggesting that an increase in intracellular pH (pHi ) upon spawning triggers the elongation of poly(A) tails. Using a cell-free system made from maturing oocytes at metaphase I, we demonstrated that polyadenylation was indeed suppressed at pH below 7.0. These results suggest that a pH-sensitive process, functioning after CPEB phosphorylation, is blocked under physiologically low pHi (<7.0) in metaphase-I-arrested oocytes. The increase in pHi (>7.0) that occurs after spawning triggers polyadenylation of cyclin B mRNA and progression into meiosis II.

journal_name

Mol Reprod Dev

authors

Ochi H,Aoto S,Tachibana K,Hara M,Chiba K

doi

10.1002/mrd.22599

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

79-87

issue

1

eissn

1040-452X

issn

1098-2795

journal_volume

83

pub_type

杂志文章
  • DNA hypomethylation of karyoplasts for bovine nuclear transplantation.

    abstract::The objective of this research was to evaluate if DNA hypomethylation in cells used as karyoplasts would improve development of bovine nuclear transplantation (NT) embryos. DNA from serum-fed (SF), serum-starved (SS), and 1, or 5 microM 5-azacytidine (5-aza-CR) treated cells was digested with a methylation sensitive e...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1079

    authors: Jones KL,Hill J,Shin TY,Lui L,Westhusin M

    更新日期:2001-10-01 00:00:00

  • Developmentally regulated expression during gametogenesis of the murine gene meg1 suggests a role in meiosis.

    abstract::Previous studies have shown that in adult male mice, expression of the meg1 gene is restricted to meiotic and early postmeiotic testicular germ cells. We have now analyzed the expression of meg1 during postnatal testicular development and the comparable meiotic stages in the female. The 0.75 kb transcript for meg1 beg...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080380104

    authors: Don J,Winer MA,Wolgemuth DJ

    更新日期:1994-05-01 00:00:00

  • Nuclear localization signal of SV40 T antigen directs import of plasmid DNA into sea urchin male pronuclei in vitro.

    abstract::Nuclear import of plasmid DNA mediated by a nuclear localization signal (NLS) derived from SV40 T antigen was investigated in a cell-free extract. In vitro assembled sea urchin male pronuclei were incubated in a 100,000g supernatant of a zebrafish fertilized egg lysate, together with fluorescently labeled plasmid DNA ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2795(199612)45:4<431::AID-MRD4>

    authors: Collas P,Aleström P

    更新日期:1996-12-01 00:00:00

  • Ran, a GTP-binding protein involved in nucleocytoplasmic transport and microtubule nucleation, relocates from the manchette to the centrosome region during rat spermiogenesis.

    abstract::Ran, a Ras-related GTPase, is required for transporting proteins in and out of the nucleus during interphase and for regulating the assembly of microtubules. cDNA cloning shows that rat testis, like mouse testis, expresses both somatic and testis-specific forms of Ran-GTPase. The presence of a homologous testis-specif...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.10164

    authors: Kierszenbaum AL,Gil M,Rivkin E,Tres LL

    更新日期:2002-09-01 00:00:00

  • Molecular cloning and characterization of a mouse nuclear orphan receptor expressed in embryos and testes.

    abstract::A mouse cDNA encoding a putative DNA-binding protein of the zinc-finger type was isolated from an E8.5 mouse embryonic cDNA library. Sequence comparison revealed a high degree of homology between this mouse cDNA and the human and rat orphan receptor Tr2-11 isolated from prostate cDNA libraries. This transcript was det...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2795(199607)44:3<305::AID-MRD4>

    authors: Lee CH,Chang L,Wei LN

    更新日期:1996-07-01 00:00:00

  • Spermatozoa lacking acrosin protein show delayed fertilization.

    abstract::Acrosin (ACR), a serine proteinase located in the acrosome of the sperm, has been presumed to be involved in the recognition and binding of the sperm to the zona pellucida of the ovum and the sperm penetration through the zona pellucida. To examine the function of acrosin in vivo, we have generated mice carrying a mut...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2795(199703)46:3<370::AID-MRD16

    authors: Adham IM,Nayernia K,Engel W

    更新日期:1997-03-01 00:00:00

  • A freezing and thawing method of hamster oocytes designed for both the penetration test and chromosome assay of human spermatozoa.

    abstract::Superovulated hamster oocytes were cryopreserved and thawed according to our carefully designed procedures. More than 90% (92 +/- 4%) of oocytes survived freezing and thawing. They were proven to be well conserved, showing excellent performance comparable to freshly ovulated oocytes in the human sperm penetration test...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080330213

    authors: Tateno H,Kamiguchi Y,Mikamo K

    更新日期:1992-10-01 00:00:00

  • Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele.

    abstract::Varicocele is a prevalent pathology among infertile men. The mechanisms linking this condition to infertility, however, are poorly understood. Our previous work showed a relationship between sperm functional quality and the ability of spermatozoa to respond to capacitating conditions with increased membrane fluidity a...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20611

    authors: Buffone MG,Brugo-Olmedo S,Calamera JC,Verstraeten SV,Urrutia F,Grippo L,Corbetta JP,Doncel GF

    更新日期:2006-12-01 00:00:00

  • Transferrin overexpression alters testicular function in aged mice.

    abstract::Many studies have shown a correlation between transferrin (Tf) concentration and sperm yield in several mammalian species. We have used transgenic mice expressing human Tf (hTf) to investigate if overexpression of Tf increases the efficiency of mouse spermatogenesis. We demonstrated that a 36% increase of Tf does not ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20523

    authors: Lécureuil C,Staub C,Fouchécourt S,Maurel MC,Fontaine I,Martinat N,Gauthier C,Daudignon A,Delaleu B,Sow A,Jégou B,Guillou F

    更新日期:2007-02-01 00:00:00

  • Association of MPF, MAPK, and nuclear progression dynamics during activation of young and aged bovine oocytes.

    abstract::We have previously shown that bovine oocytes parthenogenetically activated after 40 hours (hr) of in vitro maturation proceed through the cell cycle faster than those after 20 hr of maturation. In the present study, we used this model of different speed of nuclear progression to investigate the correlation of two hall...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.10072

    authors: Tian XC,Lonergan P,Jeong BS,Evans AC,Yang X

    更新日期:2002-05-01 00:00:00

  • The sensitive period for male-to-female sex reversal begins at the embryonic stage in the Nile tilapia and is associated with the sexual genotype.

    abstract::In this study, we sought to determine the mechanism of early sex reversal in a teleost by applying 4 hr feminization treatments to XY (17α-ethynylestradiol 2000 μg L(-1) ) and YY (6500 μg L(-1) ) Nile tilapia embryos on the first day post-fertilization (dpf). We then searched for changes in the expression profiles of ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22436

    authors: Gennotte V,Mélard C,D'Cotta H,Baroiller JF,Rougeot C

    更新日期:2014-12-01 00:00:00

  • Izumo is part of a multiprotein family whose members form large complexes on mammalian sperm.

    abstract::Izumo, a sperm membrane protein, is essential for gamete fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N-terminal domain for which neither the functions nor homologous sequences are known. In the present work we identified three novel proteins showing an N-terminal domain with significant homology t...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.21092

    authors: Ellerman DA,Pei J,Gupta S,Snell WJ,Myles D,Primakoff P

    更新日期:2009-12-01 00:00:00

  • MicroRNA regulation of immune events at conception.

    abstract::The reproductive tract environment at conception programs the developmental trajectory of the embryo, sets the course of pregnancy, and impacts offspring phenotype and health. Despite the fundamental importance of this stage of reproduction, the rate-limiting regulatory mechanisms operating locally to control fertilit...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审

    doi:10.1002/mrd.22823

    authors: Robertson SA,Zhang B,Chan H,Sharkey DJ,Barry SC,Fullston T,Schjenken JE

    更新日期:2017-09-01 00:00:00

  • In silico mining of EST databases for novel pre-implantation embryo-specific zinc finger protein genes.

    abstract::Progress in the understanding of early mammalian embryo development has been severely hampered by scarcity of study materials. To circumvent such a constraint, we have developed a strategy that involves a combination of in silico mining of new genes from expressed sequence tags (EST) databases and rapid determination ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1029

    authors: Choo KB,Chen HH,Cheng WT,Chang HS,Wang M

    更新日期:2001-07-01 00:00:00

  • Cycle-dependent endometrial expression and hormonal regulation of the fibulin-1 gene.

    abstract::Fibulin-1 is a secreted protein associated with elastic matrix fibres and basement membranes. It plays a role in stabilizing blood vessels and can also regulate cell motility and invasiveness. We studied the regulation of the fibulin-1 gene in the rat and human endometrium, an organ where cyclic tissue remodeling and ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20079

    authors: Haendler B,Yamanouchi H,Lessey BA,Chwalisz K,Hess-Stumpp H

    更新日期:2004-07-01 00:00:00

  • Lipovitellins and phosvitins of the fertilized eggs during embryo growth in the oviparous lizard Podarcis sicula.

    abstract::In the lizard Podarcis sicula, the major vitellogenin (VTG)-derived yolk proteins, lipovitellins and phosvitins, were extracted from the yolk globules of laid and fertilized eggs at different periods of incubation up to 44 days close to hatching. Embryonic development was almost over at this time. Yolk proteins were i...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.90018

    authors: Romano M,Rosanova P,Anteo C,Limatola E

    更新日期:2002-11-01 00:00:00

  • Cloning of a novel rat placental prolactin-like protein C-related cDNA.

    abstract::Prolactin-like protein C (PLP-C) is a major rat placental protein which is expressed during the second half of pregnancy and belongs to the growth hormone-prolactin family. Here we report on the isolation of overlapping rat placental cDNAs which specify a transcript of 915 base pairs and predict a 205-amino acid trans...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080410207

    authors: Conliffe PR,Simmen RC,Buhi WC,Shiverick KT

    更新日期:1995-06-01 00:00:00

  • MCL1 is a key regulator of steroidogenesis in mouse Leydig cells.

    abstract::Myeloid cell leukemia-1 (MCL1), an anti-apoptotic member of the BCL2 family, is expressed abundantly in the testis. Previous characterization revealed that MCL1 is expressed exclusively in the Leydig cells in the mouse testis, yet what it does in these cells remains unknown. We therefore analyzed testosterone biosynth...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22614

    authors: Guang-Yu L,Hai-Yan L,Ji-Hong L,Yun-Cong M,Xue-Lian D,Chun-Yu L,Wen-Yong S

    更新日期:2016-03-01 00:00:00

  • Expression of activin receptor II and IIB mRNA isoforms in mouse reproductive organs and oocytes.

    abstract::Activins, the dimeric polypeptides of inhibin beta-subunits, exhibit paracrine effects on cell proliferation, differentiation, and various other cell functions. The complex biological response to activin appears to involve multiple receptors. In the present study, we examined the isoform mRNA expression of both activi...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080380103

    authors: Wu TC,Jih MH,Wang L,Wan YJ

    更新日期:1994-05-01 00:00:00

  • Heat stress-induced apoptosis in porcine in vitro fertilized and parthenogenetic preimplantation-stage embryos.

    abstract::Decades worth of research have consistently shown the adverse effects of elevated temperatures on reproductive parameters of livestock species. The objective of this study was to evaluate the developmental and apoptotic responses of porcine in vitro fertilized (IVF) and parthenogenetically activated (PA) embryos heat ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20620

    authors: Isom SC,Prather RS,Rucker EB 3rd

    更新日期:2007-05-01 00:00:00

  • Expression of prostatic secretory protein (PSP)-like protein in porcine corpus luteum: isolation and characterization of a new gene encoding PSP94-like protein.

    abstract::cDNA clones encoding stage-and/or space-specific genes in porcine ovarian follicles have been investigated by differential screening of cDNA libraries of granulosa cells and corpus luteum. A clone corresponding to mRNA preferentially expressed in the functional stage of corpus luteum was found to encode a protein simi...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080420204

    authors: Tanaka T,Itahana K,Andoh N,Takeya T,Sato E

    更新日期:1995-10-01 00:00:00

  • Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection.

    abstract::Recently, mice and embryonic stem (ES) cells with allelic polymorphisms have been used extensively in the field of genetics and developmental biology. In this study, we examined whether intersubspecific hybrid mice and ES cells with these genotypes can be efficiently produced by intracytoplasmic sperm injection (ICSI)...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20612

    authors: Shinmen A,Honda A,Ohkawa M,Hirose M,Ogonuki N,Yuzuriha M,Miki H,Mochida K,Inoue K,Abe K,Ito M,Ogura A

    更新日期:2007-09-01 00:00:00

  • Aberrant methylation of the IL-12B promotor region contributes to the risk of developing ovarian endometriosis.

    abstract::Studies have shown that aberrant expression of IL-12p40, which is encoded by the interleukin-12B (IL-12B) gene, may be involved in the development of endometriosis. In this study, we investigated the role of aberrant methylation of the IL-12B promoter region and its associated expression in the development of ovarian ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.23139

    authors: Zhao W,Kang S,Zhao J,Wang L,Cao S,Li Y

    更新日期:2019-06-01 00:00:00

  • Efficient and simple production of transgenic mice and rabbits using the new DMSO-sperm mediated exogenous DNA transfer method.

    abstract::A high efficient and simple transgenic technology on mice and rabbits to transfect spermatozoa with exogenous DNA/DMSO complex to obtain transgenic offspring, which is namely called DMSO-sperm mediated gene transfer (SMGT). Mouse sperm could be either directly transfected via injection into testis or cultured in vitro...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20401

    authors: Shen W,Li L,Pan Q,Min L,Dong H,Deng J

    更新日期:2006-05-01 00:00:00

  • Centrosome reduction during Rhesus spermiogenesis: gamma-tubulin, centrin, and centriole degeneration.

    abstract::Centrosome reduction during spermiogenesis has been studied using anti-gamma-tubulin and anti-centrin antibodies and electron microscopy in nonhuman primates. Rhesus spermatids possess apparently normal centrosomes comprising a pair of centrioles associated with gamma-tubulin and centrin. However, they do not nucleate...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/1098-2795(200008)56:4<502::AID-MRD8>3.0.CO

    authors: Manandhar G,Schatten G

    更新日期:2000-08-01 00:00:00

  • Estrogen receptor- and aromatase-deficient mice provide insight into the roles of estrogen within the ovary and uterus.

    abstract::Estrogen receptor (ER)- (alpha, beta, and both alpha and beta) and aromatase (Ar) knockout (KO) mice have been created to assess the biological effects of estrogens. This review article discusses the ovarian and uterine phenotypes of these mice. The data obtained have confirmed some older inferences about how the ster...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审

    doi:10.1002/mrd.1039

    authors: Rosenfeld CS,Roberts RM,Lubahn DB

    更新日期:2001-07-01 00:00:00

  • Sperm-specific AKAP3 is a dual-specificity anchoring protein that interacts with both protein kinase a regulatory subunits via conserved N-terminal amphipathic peptides.

    abstract::cAMP-dependent protein kinase A (PKA) plays important regulatory roles during mouse spermatogenesis. PKA-mediated signaling has been shown to regulate gene expression, chromatin condensation, capacitation, and motility during sperm development and behavior, although how PKA is regulated in spatiotemporal manners durin...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22329

    authors: Xu K,Qi H

    更新日期:2014-07-01 00:00:00

  • Factors affecting production of transgenic rats by ICSI-mediated DNA transfer: effects of sonication and freeze-thawing of spermatozoa, rat strains for sperm and oocyte donors, and different constructs of exogenous DNA.

    abstract::Factors affecting the efficiency of producing transgenic rats by intracytoplasmic sperm injection (ICSI)-mediated DNA transfer were investigated. Epididymal spermatozoa from Sprague-Dawley (SD) rats were sonicated and/or frozen-thawed for cutting the tail and membrane disruption. The sperm heads were exposed for 1 min...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20223

    authors: Hirabayashi M,Kato M,Ishikawa A,Kaneko R,Yagi T,Hochi S

    更新日期:2005-04-01 00:00:00

  • Repulsive guidance molecules (RGMs) and neogenin in bone morphogenetic protein (BMP) signaling.

    abstract::Bone morphogenetic proteins (BMPs) belong to the transforming growth factor-beta (TGFβ) superfamily. BMPs mediate a highly conserved signal transduction cascade through the type-I and type-II serine/threonine kinase receptors and intracellular Smad proteins, which regulate multiple developmental and homeostatic proces...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审

    doi:10.1002/mrd.22199

    authors: Tian C,Liu J

    更新日期:2013-09-01 00:00:00

  • Interspecies nuclear transfer reveals that demethylation of specific repetitive sequences is determined by recipient ooplasm but not by donor intrinsic property in cloned embryos.

    abstract::DNA methylation/demethylation of donor genomes in recipient ooplasm after nuclear transfer occurs in a species-specific way. In cloned rabbit and bovine embryos, repetitive sequences maintain the donor-type methylation status, but typical demethylation of repetitive sequences takes place in cloned porcine embryos. To ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20421

    authors: Chen T,Zhang YL,Jiang Y,Liu JH,Schatten H,Chen DY,Sun QY

    更新日期:2006-03-01 00:00:00