Abstract:
:The completion of spermiogenesis requires condensation of the haploid spermatid genome. This task is accomplished in a gradual and relentless manner by first erasing the nucleosomal organization of chromatin while the DNA is protected by transient nuclear proteins TP1 and TP2. Then, the more permanent protamines come into play to stabilize the spermatid genome until fertilization occurs. Mice lacking TPI manage to produce relatively structurally normal sperm, although fertility is reduced and chromatin condensation is abnormal despite the compensatory expression of TP2. TP1 and TP2 appear to have the house-keeping function of reestablishing continuity when chromatin breaks take place during the remodeling process. DNA single-strand breaks are frequently observed when spermiogenesis is half completed. There is a temporal relationship between TP1 and DNA breaks: TP1 nuclear levels increase and the frequency of DNA breaks become less prominent as spermiogenesis is reaching completion. TP1 seems to hold the broken ends together until an as-yet-unidentified ligase bridges the gap.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Kierszenbaum ALdoi
10.1002/1098-2795(20010401)58:4<357::AID-MRD1>3.0.keywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
357-8issue
4eissn
1040-452Xissn
1098-2795pii
10.1002/1098-2795(20010401)58:4<357::AID-MRD1>3.0.journal_volume
58pub_type
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