Abstract:
:Several proteins with phosphorylated tyrosine residues have been shown to be closely involved in the control meiotic nuclear division. We identified a 42-kD protein in pig oocytes, using a polyclonal antibody to a synthetic phosphotyrosine construct that increases significantly in amount after 12 hr of maturation culture, and is discretely localized to condensing and condensed chromosomes. However, since microinjection of the antibody into oocytes blocks spindle formation, the role of this protein appears to be at that stage rather than directly in chromosome condensation. Specificity of action of the 42-kD protein indicates that it may be a phosphorylation-dependent component necessary for successful spindle assembly.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Miyano T,Moor RM,Wooding FB,Shiroo Mdoi
10.1002/(SICI)1098-2795(199607)44:3<408::AID-MRD14subject
Has Abstractpub_date
1996-07-01 00:00:00pages
408-16issue
3eissn
1040-452Xissn
1098-2795pii
10.1002/(SICI)1098-2795(199607)44:3<408::AID-MRD14journal_volume
44pub_type
杂志文章abstract::Myeloid cell leukemia-1 (MCL1), an anti-apoptotic member of the BCL2 family, is expressed abundantly in the testis. Previous characterization revealed that MCL1 is expressed exclusively in the Leydig cells in the mouse testis, yet what it does in these cells remains unknown. We therefore analyzed testosterone biosynth...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22614
更新日期:2016-03-01 00:00:00
abstract::Acrosin (ACR), a serine proteinase located in the acrosome of the sperm, has been presumed to be involved in the recognition and binding of the sperm to the zona pellucida of the ovum and the sperm penetration through the zona pellucida. To examine the function of acrosin in vivo, we have generated mice carrying a mut...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199703)46:3<370::AID-MRD16
更新日期:1997-03-01 00:00:00
abstract::The process of chromatin condensation during spermiogenesis in Rana tigerina is similar to the heterochromatization in somatic cells, where 30 nm fibers are coalesced together into a dense mass in spermatozoa without changing their initial size and nucleosomal organization. This conclusion was supported by the finding...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20191
更新日期:2005-02-01 00:00:00
abstract::This report is part of a continuing study in which we employ monoclonal antibodies to membrane domains and internal organelles of rat spermatozoa in order to trace events during maturation, capacitation, fertilization, and early development. In the present study, we have used immunocytochemistry at the light and EM le...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080370304
更新日期:1994-03-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is the most common endocrinological pathology among women of reproductive age, and is characterized by abnormalities in ovarian angiogenesis, among other features. Consistent with this association, follicular fluid (FF) concentration and ovarian expression of vascular endothelial growt...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22343
更新日期:2014-08-01 00:00:00
abstract::Mitogen-activated protein kinase (MAPK) plays a crucial role in meiotic maturation of mouse oocytes. In order to understand the mechanism by which MAPK regulates meiotic maturation, we examined the effects of the MAPK pathway inhibitor U0126 on microtubule organization, gamma-tubulin and nuclear mitotic apparatus prot...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20695
更新日期:2007-07-01 00:00:00
abstract::The nuclear matrix is thought to be responsible for DNA organization, DNA replication, RNA synthesis, and RNA processing. We have looked for the presence of nuclear matrix antigens during early mouse embryogenesis. Antibodies to peripheral and interior antigens (P1, Pl1, Pl2, and lamin B) were used to immunolocalize n...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080320304
更新日期:1992-07-01 00:00:00
abstract::It has been reported that the MRL/MpJ mouse strain shows several unique phenotypes, including rapid wound healing, inherent collagen disease, heat shock-resistant spermatocytes, and metaphase-specific apoptosis (Msa) in the testis. In the present study, we found the genetic mutation associated with Msa by chromosomal ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10208
更新日期:2003-02-01 00:00:00
abstract::Previous work provided evidence of Na+/H+ exchanger activity in the apical domain of mouse trophectodermal plasma membranes that provides a route for entry of extracellular Na+ (Manejwala et al., 1989). This activity was hypothesized to contribute to the trans-trophectodermal Na+ flux that is required for blastocoel e...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199806)50:2<146::AID-MRD4>
更新日期:1998-06-01 00:00:00
abstract::Mammalian reproductive processes involve spermatogenesis, which occurs in the testis, and fertilization, which takes place in the female genital tract. Fertilization is a successive, multistep, and extremely complicated event that usually includes sperm survival in the uterus, sperm migration through the uterotubal ju...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.23180
更新日期:2019-08-01 00:00:00
abstract::In the present study propidium iodide was used as a fluorescent dye to stain DNA of cells of hamster testicular origin and fluorescent intensities were analyzed by flow cytometry. We used hamster testicular cells from the first spermatogenic wave to observe the consecutive appearance of the different types of cells du...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200002)55:2<205::AID-MRD10
更新日期:2000-02-01 00:00:00
abstract::Morphogenesis of the Eledone cirrhosa sperm nucleus, as studied by electron microscopic techniques, is compared with that of Octopus vulgaris. Both species of cephalopods belong to the family Octopodidae. The results indicate that extensive nuclear helicoidization during E. cirrhosa spermiogenesis is brought about by ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10124
更新日期:2002-07-01 00:00:00
abstract::The objective of this study was to evaluate the effect of progesterone (P4), estradiol (E2), and cortisol (CO) at intraoviductal concentrations on bovine embryo development and quality in vitro. After fertilization of in vitro matured oocytes, zygotes were cultured for 8 days in synthetic oviductal fluid, supplemented...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23144
更新日期:2019-06-01 00:00:00
abstract::Gonadotropin-Releasing Hormones (GnRHs) are decapeptides well known to regulate the reproductive cycle. They are expressed not only in the brain, but also in other tissues including the gonads. It is believed that they may be involved in the endocrine and paracrine regulation of the reproductive cycle. To date, two fo...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10065
更新日期:2002-05-01 00:00:00
abstract::The present study has used methoxyacetic acid (MAA)-induced depletion of specific germ cell types in the rat and in situ hybridization with nonradioactive riboprobes to determine the stages of the spermatogenic cycle at which there is expression of the mRNA for the basic chromosomal protein transition protein 2 (TP2)....
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330404
更新日期:1992-12-01 00:00:00
abstract::Inhibin α (Inha) gene expression is regulated, in rat granulosa cells, via a cyclic 3',5'-adenosine monophosphate (AMP)-response element (CRE) found in a region of the promoter that is homologous to the human INHA promoter. We previously found that during in vitro cytotrophoblast differentiation, human INHA gene expre...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22421
更新日期:2014-11-01 00:00:00
abstract::In poikilothermic vertebrates, sex determination is sometimes influenced by environmental factors such as temperature. However, little is known about the molecular mechanisms underlying environmental sex determination. The medaka (Oryzias latipes) is a teleost fish with an XX/XY sex determination system. Recently, it ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21203
更新日期:2010-08-01 00:00:00
abstract::Lactate produced by glycolysis in Sertoli cells (SCs) is the main energy substrate for developing germ cells and plays a vital role in spermatogenesis. MicroRNAs (miRNAs) function as posttranscriptional regulators of gene expression in biological processes. We have previously shown that hyperthermia (43°C, 30 min) pro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23265
更新日期:2019-11-01 00:00:00
abstract::The objective of the present study was to determine whether glucocorticoid (GC) and its receptor (GC-R) are expressed in the porcine corpus luteum (CL), and whether GC influences porcine luteal hormone production. The gene expressions of 11beta-hydroxysteroid dehydrogenase type 1 (11-HSD1), type 2 (11-HSD2), GC-R, and...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20833
更新日期:2008-05-01 00:00:00
abstract::Decades worth of research have consistently shown the adverse effects of elevated temperatures on reproductive parameters of livestock species. The objective of this study was to evaluate the developmental and apoptotic responses of porcine in vitro fertilized (IVF) and parthenogenetically activated (PA) embryos heat ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20620
更新日期:2007-05-01 00:00:00
abstract::Endometriosis is defined as endometrial tissue outside of the uterine cavity. The pathogenesis of this common disease remains poorly understood. However, the implantation and invasion of the viable cells from retrograde menstruation into the peritoneum is a widely accepted theory. To date, the mechanisms by which cell...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10121
更新日期:2002-07-01 00:00:00
abstract::Successful pregnancy depends on the precise regulation of extravillous trophoblast cell invasion ability. MicroRNA-210-3p (miR-210), which is increased in the placenta of pre-eclampsia. Furthermore, miR-210 could inhibit trophoblasts invasion and might act as a serum biomarker for pre-eclampsia. Previous studies have ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23154
更新日期:2019-07-01 00:00:00
abstract::Hoxa10, a homeodomain transcription factor, is dynamically expressed in adult uterine endometrium where it is necessary for embryo implantation. Endometrial Hoxa10 expression is driven by estrogen and progesterone. High levels of endometrial Hoxa10 expression coincide with high progesterone levels and development of e...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20013
更新日期:2004-01-01 00:00:00
abstract::The highly conserved Pumilio protein plays crucial roles in fertility of many organisms acting as a repressor of translation, and causing infertility when mutated. Although one of two human Pumilio homologs, PUMILIO2 is expressed mainly in the germ line, its role in mammalian germ cell development has not been reporte...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20683
更新日期:2007-06-01 00:00:00
abstract::Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20164
更新日期:2004-10-01 00:00:00
abstract::Annexins are a family of Ca(2+)-binding proteins involved in the exocytotic process. The presence and the role of annexins in mammalian spermatozoa have not been well established. Two annexin-like proteins were obtained from guinea pig testis, a doublet of Mr 31-33 kD (p31/33) and a protein of Mr 50 kD (p50). Both pro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199603)43:3<366::AID-MRD11
更新日期:1996-03-01 00:00:00
abstract::Connexin 43 (Cx43), a gap junction protein expressed in differentiated granulosa cells, is necessary for normal follicular development. Cx43 expression and regulation by epidermal growth factor (EGF) were characterized in immature rabbit granulosa cells. Cx43 mRNA was expressed in the granulosa cells of primary follic...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10219
更新日期:2003-01-01 00:00:00
abstract::Germinal vesicle (GV) breakdown in mammalian oocytes is regulated by the activation of maturation promoting factor (MPF). We investigated a specific cdc2 kinase inhibitor, roscovitine, to maintain pig oocytes in the GV stage. Cumulus-oocyte complexes (COCs) were aspirated from slaughterhouse ovaries and cultured for 4...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10234
更新日期:2003-04-01 00:00:00
abstract::The mussel Mytilus edulis can be used as model to study the molecular basis of reproductive isolation because this species maintains its species integrity, despite of hybridizing in zones of contact with the closely related species M. trossulus or M. galloprovincialis. This study uses selective antibody production by ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20916
更新日期:2009-01-01 00:00:00
abstract::We hypothesized that the testis-specific isoform of angiotensin-converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti-tACE antibody; (ii) characterize expression of tACE in bovine tes...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22790
更新日期:2017-05-01 00:00:00