Abstract：
:Decades worth of research have consistently shown the adverse effects of elevated temperatures on reproductive parameters of livestock species. The objective of this study was to evaluate the developmental and apoptotic responses of porcine in vitro fertilized (IVF) and parthenogenetically activated (PA) embryos heat stressed at the late 1-cell stage. Embryos were heat stressed (HS) at 42 degrees C for 9 hr starting 22 hr after insemination or artificial activation stimulus. Non heat-stressed (NHS) control embryos were maintained at 39 degrees C for the duration of the experiments. TUNEL staining on Day 5 of development demonstrated that heat stress elicited a significant apoptotic response in IVF embryos (45.6% of HS embryos and 26.7% of NHS embryos were apoptotic; P<0.05), but not in PA embryos (51.1% and 39.9% for HS and NHS embryos, respectively; P>0.1). And, while IVF embryos were highly susceptible to heat-induced developmental perturbations (20.6% and 8.8% development to blastocyst for NHS and HS embryos, respectively; P<0.05), elevated temperatures did not affect blastocyst rates in PA embryos (22.2% for NHS PA embryos and 21.2% for HS PA embryos; P>0.1). These findings indicate that, as in other systems studied, IVF pig embryos are directly affected adversely by heat stress conditions. Parthenogenetic embryos, though, appear to be surprisingly tolerant of the elevated temperatures. The differences between IVF and PA embryos in their response to heat stress warrants further investigation.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Isom SC,Prather RS,Rucker EB 3rddoi
10.1002/mrd.20620subject
Has Abstractpub_date
2007-05-01 00:00:00pages
574-81issue
5eissn
1040-452Xissn
1098-2795journal_volume
74pub_type
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2012-11-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080270212
更新日期：1990-10-01 00:00:00
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更新日期：2007-01-01 00:00:00
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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更新日期：2007-03-01 00:00:00
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pub_type： 杂志文章
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
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更新日期：2014-07-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
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更新日期：1990-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/mrd.1080420204
更新日期：1995-10-01 00:00:00
