Production, characterization, and immunocytochemical applications of monoclonal antibodies to human sperm protamines.

abstract：:Fully grown immature oocytes acquire the ability to be fertilized with sperm after meiotic maturation, which is finally accomplished by the formation and activation of the maturation-promoting factor (MPF). MPF is the complex of Cdc2 and cyclin B, and its function in promoting metaphase is common among species. The Mo...

journal_title：Molecular reproduction and development

authors： Yoshida N,Mita K,Yamashita M

更新日期：2000-09-01 00:00:00

abstract：:The present study aims at quantification of gonadotropin releasing hormone (GnRH) by radioimmunoassay, relative expression of its mRNA by real-time PCR accompanied by its cellular localization in the rat ovary by immunonohistochemistry (IHC) during different time points of pregnancy. To determine the involvement of en...

journal_title：Molecular reproduction and development

authors： Sengupta A,Chakrabarti N,Sridaran R

更新日期：2008-06-01 00:00:00

abstract：:In newt testis, spermatocytes never appear during winter, because secondary spermatogonia die by apoptosis just before meiosis. In the current study, we examined the effect of low temperatures on spermatogenesis. Incubation of newts at low temperatures (8, 12, 15 degrees C) induced defects in spermatogenesis in a temp...

journal_title：Molecular reproduction and development

authors： Yazawa T,Nakayama Y,Fujimoto K,Matsuda Y,Abe K,Kitano T,Abé S,Yamamoto T

更新日期：2003-09-01 00:00:00

abstract：:Altering incubation temperature during embryogenesis has an impact on chicken embryo growth, but the underlying molecular mechanisms are not understood; the present study was performed to address these changes. Broiler eggs were incubated at low (36.8°C), control (37.8°C), and high (38.8°C) temperatures between Embryo...

journal_title：Molecular reproduction and development

authors： Krischek C,Janisch S,Naraballobh W,Brunner R,Wimmers K,Wicke M

更新日期：2016-01-01 00:00:00

abstract：:Ovulated rat oocytes undergo spontaneous activation during in vitro culture. After extrusion of the second polar body, they do not enter interphase but are arrested again in next metaphase-like stage (M III arrest). The present study demonstrates that puromycin and chloral hydrate can trigger transition to interphase ...

journal_title：Molecular reproduction and development

authors： Zernicka-Goetz M

更新日期：1991-02-01 00:00:00

abstract：:The mouse metallothionein (MT) gene family consists of four known members (MT-I through IV) clustered on chromosome 8. Studies reported herein examine the expression and regulation of the MT-III and MT-IV genes in specific cell types in the maternal reproductive tract, developing embryo, and fetus known to express the...

journal_title：Molecular reproduction and development

authors： Liang L,Fu K,Lee DK,Sobieski RJ,Dalton T,Andrews GK

更新日期：1996-01-01 00:00:00

abstract：:Fibulin-1 is a secreted protein associated with elastic matrix fibres and basement membranes. It plays a role in stabilizing blood vessels and can also regulate cell motility and invasiveness. We studied the regulation of the fibulin-1 gene in the rat and human endometrium, an organ where cyclic tissue remodeling and ...

journal_title：Molecular reproduction and development

authors： Haendler B,Yamanouchi H,Lessey BA,Chwalisz K,Hess-Stumpp H

更新日期：2004-07-01 00:00:00

abstract：:A group of low Mr (16 kDa-23 kDa) glycoproteins on ejaculated boar spermatozoa have been shown to have high affinity for homologous zona pellucida glycoproteins (ZPGPs). These ZPGP binding proteins are derived from seminal plasma as shown by their absence from epididymal spermatozoa and their presence in seminal plasm...

journal_title：Molecular reproduction and development

authors： Parry RV,Barker PJ,Jones R

更新日期：1992-09-01 00:00:00

abstract：:Global warming represents a major stressful environmental condition that compromises the reproductive efficiency of animals and humans via a rise of body temperature above its physiological homeothermic point (heat stress [HS]). The injuries caused by HS on reproductive function involves both male and female component...

journal_title：Molecular reproduction and development

authors： Boni R

更新日期：2019-10-01 00:00:00

abstract：:In the dasyurid marsupial, Sminthopsis crassicaudata, the mature spermatozoon has an inner homogeneous (C1) and a peripheral indented (C2) region. Using DNase-gold conjugates, and biotinylated genomic DNA probes, DNA was found to occur in both C1 and C2 regions. The morphogenesis of the spermatozoon nucleus was invest...

journal_title：Molecular reproduction and development

authors： Soon LL,Breed WG

更新日期：1996-02-01 00:00:00

abstract：:Fresh cytosols extracted from unfertilized amphibian eggs contain a cytostatic factor (CSF) which arrests the cell cycle at metaphase when microinjected into cleaving blastomeres. This CSF is sensitive to Ca2+, and is designated primary CSF (1 degree CSF). During storage of Ca(2+)-containing cytosols at 2 degrees C, s...

journal_title：Molecular reproduction and development

authors： Zhang J,Masui Y

更新日期：1997-07-01 00:00:00

abstract：:Primordial germ cells (PGCs) are useful for producing transgenic chickens and preserving genetic material in avian species. In this study, we investigated the in vitro differentiation potential of chicken PGCs into different cell types. For differentiation into adipocytes, chicken PGCs were cultured for 21 days in ind...

journal_title：Molecular reproduction and development

authors： Li BC,Tian ZQ,Sun M,Xu Q,Wang XY,Qin YR,Xu F,Gao B,Wang KH,Sun HC,Chen GH

更新日期：2010-09-01 00:00:00

abstract：:A full-length cDNA encoding the bovine transforming growth factor beta (TGF-beta) receptor type I (bT beta R-I) was isolated from a placenta cDNA library. The deduced protein sequence of 499 residues contains a single transmembrane domain, a cysteinerich extracellular domain, and an intracellular kinase domain with pr...

journal_title：Molecular reproduction and development

authors： Roelen BA,Van Eijk MJ,Van Rooijen MA,Bevers MM,Larson JH,Lewin HA,Mummery CL

更新日期：1998-01-01 00:00:00

abstract：:Topoisomerase II (topo II) is an essential enzyme that alters DNA topology. This activity is important for a variety of chromosome functions including DNA replication, transcription, recombination, and chromosome condensation and segregation. Previously we localized topo II in mouse gametes and preimplantation embryos...

journal_title：Molecular reproduction and development

authors： St Pierre J,Wright DJ,Rowe TC,Wright SJ

更新日期：2002-03-01 00:00:00

abstract：:Post-acrosomal WW-domain binding protein (PAWP) is a signaling molecule located in the post-acrosomal sheath (PAS) of mammalian spermatozoa. We hypothesized that the proper integration of PAWP in the sperm PAS is reflective of bull-sperm quality and fertility. Cryopreserved semen samples from 298 sires of acceptable, ...

journal_title：Molecular reproduction and development

authors： Kennedy CE,Krieger KB,Sutovsky M,Xu W,Vargovič P,Didion BA,Ellersieck MR,Hennessy ME,Verstegen J,Oko R,Sutovsky P

更新日期：2014-05-01 00:00:00

abstract：:Tektins are microtubule-associated cytoskeletal proteins that are expressed primarily in the male germ cell-lineage in centrioles and basal bodies and within ciliary and flagellar doublet microtubules. They are proposed to be important for axonemal architecture and microtubule stability in the sperm tail and in other ...

journal_title：Molecular reproduction and development

authors： Roy A,Yan W,Burns KH,Matzuk MM

更新日期：2004-03-01 00:00:00

abstract：:Decades worth of research have consistently shown the adverse effects of elevated temperatures on reproductive parameters of livestock species. The objective of this study was to evaluate the developmental and apoptotic responses of porcine in vitro fertilized (IVF) and parthenogenetically activated (PA) embryos heat ...

journal_title：Molecular reproduction and development

authors： Isom SC,Prather RS,Rucker EB 3rd

更新日期：2007-05-01 00:00:00

abstract：:Plac1 is a recently identified, X-linked gene whose expression is restricted primarily to cells of the trophoblast lineage. It localizes to a chromosomal locus previously implicated in placental growth. We therefore sought to determine if Plac1 is necessary for placental and embryonic development by examining a mutant...

journal_title：Molecular reproduction and development

authors： Jackman SM,Kong X,Fant ME

更新日期：2012-08-01 00:00:00

abstract：:To establish reliable criteria for the evaluation of nuclear donor embryos, we studied the effect of cell number and cell size of in vitro produced day 6 donor morulae on the rate of blastocyst formation following nuclear transfer to in vitro matured oocytes. In experiment 1, donor embryos were divided into three grou...

journal_title：Molecular reproduction and development

authors： Zakhartchenko V,Wolf E,Palma GA,Brem G

更新日期：1995-09-01 00:00:00

abstract：:The dysregulation of microRNAs (miRNAs) plays an important role in asthenozoospermia. This study evaluated the sperm microRNA-423-5p (miR-423-5p) expression in asthenozoospermia and normozoospermia, exploring the role of miR-423-5p in asthenozoospermia. Eighty participants were divided into asthenozoospermic (AZS, n =...

journal_title：Molecular reproduction and development

authors： Zhang R,Zuo Y,Cao S

更新日期：2021-01-28 00:00:00

abstract：:Forward-motility-stimulating factor (FMSF) is a protein, originally purified from bubaline serum, that promotes progressive motility of mature spermatozoa. FMSF binds to sperm surface receptors and activates transmembrane adenylyl cyclase (tmAC), causing a rise in intracellular cyclic AMP level ([cAMP]i) and subsequen...

journal_title：Molecular reproduction and development

authors： Dey S,Roy D,Majumder GC,Mukherjee B,Bhattacharyya D

更新日期：2015-12-01 00:00:00

abstract：:The expression of both inhibin alpha- and inhibin/activin beta A-subunit mRNA was examined in equine uteroplacental tissues collected during pregnancy (days 90 to 300). Northern blot analysis revealed that 5 transcripts (7.0, 4.1, 3.4, 2.6, 1.5 kb) of beta A-subunit were present, and the most abundantly expressed tran...

journal_title：Molecular reproduction and development

authors： Yamanouchi K,Hirasawa K,Hasegawa T,Ikeda A,Chang KT,Matsuyama S,Nishihara M,Miyazawa K,Sawasaki T,Tojo H,Tachi C,Takahashi M

更新日期：1997-08-01 00:00:00

abstract：:To study possible role and regulation of apoptosis occurred in primate endometrium, the expression of apoptosis-related molecules, Fas, FasL, B cell lymphoma/leukaemia-2 (Bcl-2), and Bax were analyzed in relation to occurrence of apoptosis and proliferation in the cycling endometrium of the rhesus monkey using immunoh...

journal_title：Molecular reproduction and development

authors： Wei P,Jin X,Tao SX,Han CS,Liu YX

更新日期：2005-04-01 00:00:00

abstract：:Pigs have a unique, non-invasive epitheliochorial placenta where maternal and fetal layers lay in apposition. Indentation of fetal capillaries into the trophoblasts and maternal capillaries into the uterine epithelium reduce the distance between the fetal and maternal blood, ensuring nutrient transfer for proper conce...

journal_title：Molecular reproduction and development

authors： Bidarimath M,Tayade C

更新日期：2017-09-01 00:00:00

abstract：:Semen analysis lacks a functional component and best identifies extreme cases of infertility. The ganglioside GM1 is known to have functional roles during capacitation and acrosome exocytosis. Here, we assessed whether GM1 localization patterns (Cap-Score™) correspond with male fertility in different settings: Study 1...

journal_title：Molecular reproduction and development

authors： Cardona C,Neri QV,Simpson AJ,Moody MA,Ostermeier GC,Seaman EK,Paniza T,Rosenwaks Z,Palermo GD,Travis AJ

更新日期：2017-05-01 00:00:00

abstract：:Irregular uterine bleeding is a major side effect of long-acting progestogen-only contraceptives in women, and is the primary reason women discontinue their use. In this study, a mouse model of endometrial breakdown was established using a subcutaneous progesterone implant to understand how irregular bleeding begins. ...

journal_title：Molecular reproduction and development

authors： Zhang GH,Cui LJ,Li AY,Zhang JP,Liu Y,Zhao JS,Xu XB,He B,Wang JD,Chu L,Li YF

更新日期：2016-09-01 00:00:00

abstract：:The cytoplasmic beta-actin promoter, commonly used as strong promoter in many gene regulation studies, produces a pattern of male germ cell and preimplantation, embryonic gene expression in transgenic mice. In seven of ten expressing transgenic lines, a chicken beta-actin-lacZ fusion gene was expressed in adult testes...

journal_title：Molecular reproduction and development

authors： Sands AT,Hansen TN,Demayo FJ,Stanley LA,Xin L,Schwartz RJ

更新日期：1993-02-01 00:00:00

abstract：:We have previously described a hamster sperm glycoprotein, P26h, which is implicated in the cascade of events occurring during the interaction between mature spermatozoa and the oocyte's zona pellucida. The P26h is acquired on the acrosomal cap of the spermatozoon during its maturation arising within the epididymis. L...

journal_title：Molecular reproduction and development

authors： Lamontagne N,Légaré C,Gaudreault C,Sullivan R

更新日期：2001-08-01 00:00:00

abstract：:The expression of stem cell factor (SCF) mRNA and protein was examined in the postnatal rat testis. Northern blot analysis of total RNA from 1-4 days postpartum (dpp) testes showed none or barely detectable levels of the approximately 6.5 kb SCF transcript. At 5 dpp, there was a striking elevation in this mRNA, a timi...

journal_title：Molecular reproduction and development

authors： Munsie M,Schlatt S,deKretser DM,Loveland KL

更新日期：1997-05-01 00:00:00

abstract：:Epigenetics includes the study of external factors that can influence the expression of genes by altering the accessibility of DNA through methylation. To investigate the epigenetic influence of season, sperm head shape, and semen storage on placental and fetal tissues, pregnancies were generated in the summer or wint...

journal_title：Molecular reproduction and development

authors： Rempel LA,Krautkramer MM,Parrish JJ,Miles JR

更新日期：2019-04-01 00:00:00